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The map label for this gene is ilvE [H]

Identifier: 73538112

GI number: 73538112

Start: 939157

End: 940251

Strand: Direct

Name: ilvE [H]

Synonym: Reut_B4282

Alternate gene names: 73538112

Gene position: 939157-940251 (Clockwise)

Preceding gene: 73538111

Following gene: 73538114

Centisome position: 34.45

GC content: 65.66

Gene sequence:

>1095_bases
ATGAGCGCTGACAACCAATTGCAGTTCACCGTGGAACGTCACCCCAACCCCACGCCGGCCGACAAGATCGCGGCTGCGCT
GGTCGACCCTGCCTTCGGACGCGTGTTCACCGACCACATGGTCACAATCCGCTGGACCGAAGGGCGCGGTTGGCATGACG
CCAAGGTCGAAGCGCGCCGTCCATTCCAGATCGATCCCGCCTGTGCCGTCCTGCACTATGCGCAGGAAATCTTTGAGGGC
ATGAAGGCCTATCGCGGCGCCGACGGCAAGATTTCGCTGTTCCGCCCGCAGGAAAACGCCAAGCGCTTCCGTGCGTCGGC
CGCGCGCATGTCGATGGCCGACCTCCCTGAGGCCGAGTTCCTGCGCGCTGTCGAAGCGCTCGTCGACATCGACCGCGACT
GGATCCCCGGCGGCGACGGCAGCCTGTACCTGCGCCCGTTCATGTTTGCTGCCGAGAACTTCCTCGGCGTGCGCGCCGCG
AACGAATACGTGTTCTGCGTAATCGCCTGCCCGGTCGGCCCGTACTTCAAGGGTGGCAAGTCGGCGGTTACGGTTTGGGT
GTCCGACCAGTACACGCGCGCCGCGCCCGGCGGCACCGGCGCGGCCAAGTGCGGCGGCAACTATGCGGCCAGCCTGATCG
CACAGACCGAAGCTGCACAGAAGGGCTGCGACCAGGTGGTCTTCCTTGACGCCGCGGAACACCGCTGGATCGAGGAACTC
GGCGGCATGAACGTGTTCTTCGTGATGGATGACGGCTCGCTGCTGACGCCGCCGCTGTCCGGCACGATCTTGCCCGGCAT
CACGCGCGCATCGGTGATCGAACTGGCCCGCCATGAAGGCATCAAGGTGTCGGAAACCCCGTACGCGTTCGAGGCGTGGC
AAGCCGATGCCGCGAGCGGCCGCGTGAAGGAAACGTTCGCCTGCGGCACCGCCGCCGTGGTGACTGCCATCGGCACGGTG
CGCCATGCCGGCGGTGAATTCAACATCGGCAATGGCGGCGAAGGCGAAGTCACGAAGCGCCTGCGTGCGCTGCTGACCGG
TATCCAGCGCGGCAAGGAAGCAGATACCTTCGGCTGGGTTCACCACATCGCCTGA

Upstream 100 bases:

>100_bases
GCGCGAATATCGCCCCTTTCCGCACCCGCCCGGGACTCCCTTGCATGATTTGCACCGGAAAAACTGAAATCCCTGTCCGA
AGACGAAGAGACACAGAAAC

Downstream 100 bases:

>100_bases
GGCCGGCCCCCTGCCCGTTCGTGACCCGAGCTGACCGGGTCACGAACGCGCCAGCAACCCGGGCAGCGAGGACACCAGCA
GCACGACGCCGGACGCAGTG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSADNQLQFTVERHPNPTPADKIAAALVDPAFGRVFTDHMVTIRWTEGRGWHDAKVEARRPFQIDPACAVLHYAQEIFEG
MKAYRGADGKISLFRPQENAKRFRASAARMSMADLPEAEFLRAVEALVDIDRDWIPGGDGSLYLRPFMFAAENFLGVRAA
NEYVFCVIACPVGPYFKGGKSAVTVWVSDQYTRAAPGGTGAAKCGGNYAASLIAQTEAAQKGCDQVVFLDAAEHRWIEEL
GGMNVFFVMDDGSLLTPPLSGTILPGITRASVIELARHEGIKVSETPYAFEAWQADAASGRVKETFACGTAAVVTAIGTV
RHAGGEFNIGNGGEGEVTKRLRALLTGIQRGKEADTFGWVHHIA

Sequences:

>Translated_364_residues
MSADNQLQFTVERHPNPTPADKIAAALVDPAFGRVFTDHMVTIRWTEGRGWHDAKVEARRPFQIDPACAVLHYAQEIFEG
MKAYRGADGKISLFRPQENAKRFRASAARMSMADLPEAEFLRAVEALVDIDRDWIPGGDGSLYLRPFMFAAENFLGVRAA
NEYVFCVIACPVGPYFKGGKSAVTVWVSDQYTRAAPGGTGAAKCGGNYAASLIAQTEAAQKGCDQVVFLDAAEHRWIEEL
GGMNVFFVMDDGSLLTPPLSGTILPGITRASVIELARHEGIKVSETPYAFEAWQADAASGRVKETFACGTAAVVTAIGTV
RHAGGEFNIGNGGEGEVTKRLRALLTGIQRGKEADTFGWVHHIA
>Mature_363_residues
SADNQLQFTVERHPNPTPADKIAAALVDPAFGRVFTDHMVTIRWTEGRGWHDAKVEARRPFQIDPACAVLHYAQEIFEGM
KAYRGADGKISLFRPQENAKRFRASAARMSMADLPEAEFLRAVEALVDIDRDWIPGGDGSLYLRPFMFAAENFLGVRAAN
EYVFCVIACPVGPYFKGGKSAVTVWVSDQYTRAAPGGTGAAKCGGNYAASLIAQTEAAQKGCDQVVFLDAAEHRWIEELG
GMNVFFVMDDGSLLTPPLSGTILPGITRASVIELARHEGIKVSETPYAFEAWQADAASGRVKETFACGTAAVVTAIGTVR
HAGGEFNIGNGGEGEVTKRLRALLTGIQRGKEADTFGWVHHIA

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=347, Percent_Identity=39.7694524495677, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI38176287, Length=347, Percent_Identity=39.7694524495677, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI296010904, Length=331, Percent_Identity=39.8791540785499, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI50658084, Length=361, Percent_Identity=35.4570637119114, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI296010902, Length=331, Percent_Identity=34.7432024169184, Blast_Score=197, Evalue=9e-51,
Organism=Homo sapiens, GI296010900, Length=331, Percent_Identity=34.7432024169184, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI258614015, Length=287, Percent_Identity=36.5853658536585, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI48994963, Length=324, Percent_Identity=33.641975308642, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17568601, Length=305, Percent_Identity=40.655737704918, Blast_Score=227, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI17565728, Length=341, Percent_Identity=38.1231671554252, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6322608, Length=362, Percent_Identity=36.7403314917127, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6322002, Length=361, Percent_Identity=37.1191135734072, Blast_Score=225, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24641779, Length=330, Percent_Identity=41.5151515151515, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39383; Mature: 39252

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADNQLQFTVERHPNPTPADKIAAALVDPAFGRVFTDHMVTIRWTEGRGWHDAKVEARR
CCCCCEEEEEEECCCCCCCHHHHHHHHHCHHHHHHEECCEEEEEEECCCCCCCCCCCCCC
PFQIDPACAVLHYAQEIFEGMKAYRGADGKISLFRPQENAKRFRASAARMSMADLPEAEF
CEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHH
LRAVEALVDIDRDWIPGGDGSLYLRPFMFAAENFLGVRAANEYVFCVIACPVGPYFKGGK
HHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHEEEECCCEEEEEEECCCCCCCCCCC
SAVTVWVSDQYTRAAPGGTGAAKCGGNYAASLIAQTEAAQKGCDQVVFLDAAEHRWIEEL
EEEEEEEECCEEECCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHH
GGMNVFFVMDDGSLLTPPLSGTILPGITRASVIELARHEGIKVSETPYAFEAWQADAASG
CCCEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCHHHHCCCCCCC
RVKETFACGTAAVVTAIGTVRHAGGEFNIGNGGEGEVTKRLRALLTGIQRGKEADTFGWV
CCHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
HHIA
HHCC
>Mature Secondary Structure 
SADNQLQFTVERHPNPTPADKIAAALVDPAFGRVFTDHMVTIRWTEGRGWHDAKVEARR
CCCCEEEEEEECCCCCCCHHHHHHHHHCHHHHHHEECCEEEEEEECCCCCCCCCCCCCC
PFQIDPACAVLHYAQEIFEGMKAYRGADGKISLFRPQENAKRFRASAARMSMADLPEAEF
CEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHH
LRAVEALVDIDRDWIPGGDGSLYLRPFMFAAENFLGVRAANEYVFCVIACPVGPYFKGGK
HHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHEEEECCCEEEEEEECCCCCCCCCCC
SAVTVWVSDQYTRAAPGGTGAAKCGGNYAASLIAQTEAAQKGCDQVVFLDAAEHRWIEEL
EEEEEEEECCEEECCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHH
GGMNVFFVMDDGSLLTPPLSGTILPGITRASVIELARHEGIKVSETPYAFEAWQADAASG
CCCEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCHHHHCCCCCCC
RVKETFACGTAAVVTAIGTVRHAGGEFNIGNGGEGEVTKRLRALLTGIQRGKEADTFGWV
CCHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
HHIA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]