BacMap

Definition	Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence.
Accession	NC_007348
Length	2,726,152

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The map label for this gene is crt [H]

Identifier: 73538090

GI number: 73538090

Start: 917661

End: 918431

Strand: Direct

Name: crt [H]

Synonym: Reut_B4260

Alternate gene names: 73538090

Gene position: 917661-918431 (Clockwise)

Preceding gene: 73538089

Following gene: 73538091

Centisome position: 33.66

GC content: 67.19

Gene sequence:

>771_bases
ATGATTGAATTTTCCTTGGACGGGCATATCGCCCGTCTCACGCTGAGCCGCCCGCCCGCCAATGCATTCACCGCAGAGGG
CCTGCAGCAATTGCAGGACCTGATTGCGAAGATCGACGCGAATCCGGAAGTCCGCGCCGTTGTCATCACCGGCGCGGGTG
ATCGCTTCTTCAGCGCGGGCGCCGACCTGAACGGCTTCGCCGAAGGCGACCGCGCCCATGCACGTGTGATGGCACAGCGC
TTCGGCAGTGCCTTCGAGGCGCTGCAGAATGCGCGGCCGCTCGTGATTGCCGCGATCAACGGCTATGCCATGGGCGGCGG
ACTGGAATGCGCGCTCGCCTGCGATGTGCGCATCGCGGAGGAGCAGGCGCAGATGGCACTGCCCGAAGCCGCCGTGGGGC
TGTTGCCATGCGGCTGTGGCACGCAAACCCTGCCGTGGTTGGTCGGCGAAGGCTGGGCCAAGCGGATGATCCTGACCAAT
GAGCGTGTGGATGCGGCCACGGCGCTGCGCATCGGACTGGTGGAAGAGGTGGTGCCGCGCGGCAAGGCGCTGGACACTGC
ACTGGCCATGGCCGAACGCGCGTGCGCCGTCAGTCCGCGCGCGGCGGCCTACAGTAAGCAGCTTGTGCACCTGTCGCGCC
AGGGCGTGCCGCGCCAGGCAGCGTTTGCGCTGGAGCGCGAGCGCTTTGTCGACCTGTTCGACGGCGAGGACCAGCGCGAA
GGCGTCAATGCCTTCCTGCAGAAGCGCGCGCCGCAGTGGCGCAATGCGTAG

Upstream 100 bases:

>100_bases
GATCTATCAGGCGATGAGTCAGGCCGGCGATGGCCAGCTCGATTTCTCCGGCGTGATCCGGCAATACCAGCCCAAGTCCG
GGGCCTGACGGAGGCGACTG

Downstream 100 bases:

>100_bases
GAGCGCATCATGCGACTGACACCTGAAATCGCGCCGGGCAGTTCACTGGCCGACACCGAGCCCGAAGTCCTGTTTCAGGT
CATCAACCGCGTCGGGTTGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MIEFSLDGHIARLTLSRPPANAFTAEGLQQLQDLIAKIDANPEVRAVVITGAGDRFFSAGADLNGFAEGDRAHARVMAQR
FGSAFEALQNARPLVIAAINGYAMGGGLECALACDVRIAEEQAQMALPEAAVGLLPCGCGTQTLPWLVGEGWAKRMILTN
ERVDAATALRIGLVEEVVPRGKALDTALAMAERACAVSPRAAAYSKQLVHLSRQGVPRQAAFALERERFVDLFDGEDQRE
GVNAFLQKRAPQWRNA

Sequences:

>Translated_256_residues
MIEFSLDGHIARLTLSRPPANAFTAEGLQQLQDLIAKIDANPEVRAVVITGAGDRFFSAGADLNGFAEGDRAHARVMAQR
FGSAFEALQNARPLVIAAINGYAMGGGLECALACDVRIAEEQAQMALPEAAVGLLPCGCGTQTLPWLVGEGWAKRMILTN
ERVDAATALRIGLVEEVVPRGKALDTALAMAERACAVSPRAAAYSKQLVHLSRQGVPRQAAFALERERFVDLFDGEDQRE
GVNAFLQKRAPQWRNA
>Mature_256_residues
MIEFSLDGHIARLTLSRPPANAFTAEGLQQLQDLIAKIDANPEVRAVVITGAGDRFFSAGADLNGFAEGDRAHARVMAQR
FGSAFEALQNARPLVIAAINGYAMGGGLECALACDVRIAEEQAQMALPEAAVGLLPCGCGTQTLPWLVGEGWAKRMILTN
ERVDAATALRIGLVEEVVPRGKALDTALAMAERACAVSPRAAAYSKQLVHLSRQGVPRQAAFALERERFVDLFDGEDQRE
GVNAFLQKRAPQWRNA

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=32.4, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI68989263, Length=194, Percent_Identity=37.1134020618557, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=30.3846153846154, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI20127408, Length=189, Percent_Identity=32.2751322751323, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4502327, Length=238, Percent_Identity=28.9915966386555, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI37594471, Length=223, Percent_Identity=30.4932735426009, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI37594469, Length=187, Percent_Identity=32.620320855615, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI70995211, Length=219, Percent_Identity=31.5068493150685, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI213417737, Length=179, Percent_Identity=31.2849162011173, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI157694516, Length=179, Percent_Identity=31.2849162011173, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI62530384, Length=253, Percent_Identity=24.5059288537549, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI261878539, Length=91, Percent_Identity=43.956043956044, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI295842266, Length=251, Percent_Identity=23.5059760956175, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157694520, Length=155, Percent_Identity=30.3225806451613, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787659, Length=243, Percent_Identity=41.1522633744856, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=34.6303501945525, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI1788682, Length=190, Percent_Identity=38.9473684210526, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788597, Length=255, Percent_Identity=27.843137254902, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790281, Length=194, Percent_Identity=29.8969072164948, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082183, Length=248, Percent_Identity=25.8064516129032, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=31.2977099236641, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=33.0645161290323, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI25145438, Length=260, Percent_Identity=33.0769230769231, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17540714, Length=209, Percent_Identity=31.5789473684211, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=232, Percent_Identity=28.0172413793103, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=221, Percent_Identity=28.5067873303167, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=190, Percent_Identity=30.5263157894737, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17508951, Length=190, Percent_Identity=30.5263157894737, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=214, Percent_Identity=28.5046728971963, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=190, Percent_Identity=30.5263157894737, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=223, Percent_Identity=25.5605381165919, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=249, Percent_Identity=26.9076305220884, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=186, Percent_Identity=30.6451612903226, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=203, Percent_Identity=26.1083743842365, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=30.241935483871, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=30.241935483871, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=30.7392996108949, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922422, Length=247, Percent_Identity=24.2914979757085, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21357171, Length=255, Percent_Identity=29.8039215686275, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24650670, Length=187, Percent_Identity=32.620320855615, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583165, Length=207, Percent_Identity=26.0869565217391, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19920382, Length=228, Percent_Identity=28.0701754385965, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19921018, Length=206, Percent_Identity=27.1844660194175, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921000, Length=171, Percent_Identity=34.5029239766082, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24583077, Length=171, Percent_Identity=34.5029239766082, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24583079, Length=171, Percent_Identity=34.5029239766082, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550169, Length=215, Percent_Identity=27.906976744186, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24654903, Length=159, Percent_Identity=27.0440251572327, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 27496; Mature: 27496

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFSLDGHIARLTLSRPPANAFTAEGLQQLQDLIAKIDANPEVRAVVITGAGDRFFSAG
CEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHEECC
ADLNGFAEGDRAHARVMAQRFGSAFEALQNARPLVIAAINGYAMGGGLECALACDVRIAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCEEEEEECEEEHH
EQAQMALPEAAVGLLPCGCGTQTLPWLVGEGWAKRMILTNERVDAATALRIGLVEEVVPR
HHHHHHCCHHHHHEEECCCCCCCCCHHHCCCHHHHEEEECCCCCHHHHHHHHHHHHHHCC
GKALDTALAMAERACAVSPRAAAYSKQLVHLSRQGVPRQAAFALERERFVDLFDGEDQRE
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHH
GVNAFLQKRAPQWRNA
HHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIEFSLDGHIARLTLSRPPANAFTAEGLQQLQDLIAKIDANPEVRAVVITGAGDRFFSAG
CEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHEECC
ADLNGFAEGDRAHARVMAQRFGSAFEALQNARPLVIAAINGYAMGGGLECALACDVRIAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCEEEEEECEEEHH
EQAQMALPEAAVGLLPCGCGTQTLPWLVGEGWAKRMILTNERVDAATALRIGLVEEVVPR
HHHHHHCCHHHHHEEECCCCCCCCCHHHCCCHHHHEEEECCCCCHHHHHHHHHHHHHHCC
GKALDTALAMAERACAVSPRAAAYSKQLVHLSRQGVPRQAAFALERERFVDLFDGEDQRE
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHH
GVNAFLQKRAPQWRNA
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]