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The map label for this gene is phnJ [H]

Identifier: 73538019

GI number: 73538019

Start: 830602

End: 831483

Strand: Direct

Name: phnJ [H]

Synonym: Reut_B4189

Alternate gene names: 73538019

Gene position: 830602-831483 (Clockwise)

Preceding gene: 73538018

Following gene: 73538020

Centisome position: 30.47

GC content: 64.4

Gene sequence:

>882_bases
ATGAGTCAGACCAACGCCACCGTCGCCCGCGACGACCACTACAACTTTGGCTACCTGGACGAATCAACCAAGCGCATGCT
GCGCCGTGCGCTGCTGAAGGCCGTGGCGATTCCCGGCTACCAGGTCCCGTTCGGCAGCCGCGAAATGCCGCTGCCGTATG
GCTGGGGCACGGGCGGCATCCAGGTGACGGCCGCCATCATCGGCACGCAGGACGTGCTCAAGGTGATCGACCAGGGTTCG
GACGACACCACCAACGCCATCAACATCCGCCGCTTCTTCGGCCGCGTGACCGGCGTCAAGACGACCGAGCGTACCGCTGA
AGCGAGCATCATTCAGACGCGCCACCGCATTCCCGAGTCGCCGCTGCGCGCCGGGCAGGTCATGGTGTTCCAGGTGCCGA
TTCCCGAACCGCTGCGTTGGCTGGAGCCTAGCGAGCGCGAGACGCGCACCATGCACGCGCTGGCCGAATATGGCTCGATG
CACGTCAAGCTCTACGAAGACATCGCCCATCACGGCCATATCGCCACGACCTACGACTATCCGGTGATCGTCAACCAGCG
CTACATGATGCGTCCGTCGCCGATCCCGAAGTTCGACAACCCGAAGCTCGACCGCAGCCCCGCGCTGATGCTGTTCGGCG
CCGGGCGCGAGAAGCGCGTCTATGCGGTGCCGCCTTACACCTCGGTGAAGAGCCTCGATTTCGTCGACCATCCGTTCACC
GTCGAGACATGGAGCAGCTGCTGCGCCCAGTGCGGCTCGACCGACAGCTTTCTTGACGAGATCATTACCGATGATGCCGG
CACGCGCATGTTCGTGTGCTCCGACACGGAATACTGCGGCGACCGCGTTGCCGCGCAGCGGGCCGAAGGAGCCGCACAAT
GA

Upstream 100 bases:

>100_bases
AACTGCTGCGCCGCCTGCGCACCGAGCAGGAAGCCGCGGGTACGGAAGCTGCTCCTGCCGCCGCGGTATCGAGTATCCAG
CCACAAGAGGAGACGCTGGC

Downstream 100 bases:

>100_bases
GCGCCACCCCGCTGCTTTCCGTCCGCAACCTCACGCGCACCTGGGACGGCGTGCATGGCTGCCATGATGTGAGCTTCGAC
CTCTATCCCGGCGAAGTACT

Product: phosphonate metabolism PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSQTNATVARDDHYNFGYLDESTKRMLRRALLKAVAIPGYQVPFGSREMPLPYGWGTGGIQVTAAIIGTQDVLKVIDQGS
DDTTNAINIRRFFGRVTGVKTTERTAEASIIQTRHRIPESPLRAGQVMVFQVPIPEPLRWLEPSERETRTMHALAEYGSM
HVKLYEDIAHHGHIATTYDYPVIVNQRYMMRPSPIPKFDNPKLDRSPALMLFGAGREKRVYAVPPYTSVKSLDFVDHPFT
VETWSSCCAQCGSTDSFLDEIITDDAGTRMFVCSDTEYCGDRVAAQRAEGAAQ

Sequences:

>Translated_293_residues
MSQTNATVARDDHYNFGYLDESTKRMLRRALLKAVAIPGYQVPFGSREMPLPYGWGTGGIQVTAAIIGTQDVLKVIDQGS
DDTTNAINIRRFFGRVTGVKTTERTAEASIIQTRHRIPESPLRAGQVMVFQVPIPEPLRWLEPSERETRTMHALAEYGSM
HVKLYEDIAHHGHIATTYDYPVIVNQRYMMRPSPIPKFDNPKLDRSPALMLFGAGREKRVYAVPPYTSVKSLDFVDHPFT
VETWSSCCAQCGSTDSFLDEIITDDAGTRMFVCSDTEYCGDRVAAQRAEGAAQ
>Mature_292_residues
SQTNATVARDDHYNFGYLDESTKRMLRRALLKAVAIPGYQVPFGSREMPLPYGWGTGGIQVTAAIIGTQDVLKVIDQGSD
DTTNAINIRRFFGRVTGVKTTERTAEASIIQTRHRIPESPLRAGQVMVFQVPIPEPLRWLEPSERETRTMHALAEYGSMH
VKLYEDIAHHGHIATTYDYPVIVNQRYMMRPSPIPKFDNPKLDRSPALMLFGAGREKRVYAVPPYTSVKSLDFVDHPFTV
ETWSSCCAQCGSTDSFLDEIITDDAGTRMFVCSDTEYCGDRVAAQRAEGAAQ

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=273, Percent_Identity=74.3589743589744, Blast_Score=442, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 32786; Mature: 32654

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTNATVARDDHYNFGYLDESTKRMLRRALLKAVAIPGYQVPFGSREMPLPYGWGTGGI
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCE
QVTAAIIGTQDVLKVIDQGSDDTTNAINIRRFFGRVTGVKTTERTAEASIIQTRHRIPES
EEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
PLRAGQVMVFQVPIPEPLRWLEPSERETRTMHALAEYGSMHVKLYEDIAHHGHIATTYDY
CCCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHCCCEEEEECC
PVIVNQRYMMRPSPIPKFDNPKLDRSPALMLFGAGREKRVYAVPPYTSVKSLDFVDHPFT
CEEECCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCC
VETWSSCCAQCGSTDSFLDEIITDDAGTRMFVCSDTEYCGDRVAAQRAEGAAQ
HHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQTNATVARDDHYNFGYLDESTKRMLRRALLKAVAIPGYQVPFGSREMPLPYGWGTGGI
CCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCE
QVTAAIIGTQDVLKVIDQGSDDTTNAINIRRFFGRVTGVKTTERTAEASIIQTRHRIPES
EEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
PLRAGQVMVFQVPIPEPLRWLEPSERETRTMHALAEYGSMHVKLYEDIAHHGHIATTYDY
CCCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHCCCEEEEECC
PVIVNQRYMMRPSPIPKFDNPKLDRSPALMLFGAGREKRVYAVPPYTSVKSLDFVDHPFT
CEEECCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCC
VETWSSCCAQCGSTDSFLDEIITDDAGTRMFVCSDTEYCGDRVAAQRAEGAAQ
HHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]