BacMap

Definition	Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence.
Accession	NC_007348
Length	2,726,152

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The map label for this gene is phnK [H]

Identifier: 73538020

GI number: 73538020

Start: 831480

End: 832256

Strand: Direct

Name: phnK [H]

Synonym: Reut_B4190

Alternate gene names: 73538020

Gene position: 831480-832256 (Clockwise)

Preceding gene: 73538019

Following gene: 73538021

Centisome position: 30.5

GC content: 66.92

Gene sequence:

>777_bases
ATGAGCGCCACCCCGCTGCTTTCCGTCCGCAACCTCACGCGCACCTGGGACGGCGTGCATGGCTGCCATGATGTGAGCTT
CGACCTCTATCCCGGCGAAGTACTGTGCGTTGTCGGCGAATCCGGCTCGGGCAAGAGCACGCTGCTGCACGCGCTGTCGA
TGCAGGCGCCGCCGCAGCGCGGCAGTGTCAGCTACGACATGCGCGAAGCCGGTCTGACCGACCTGGCCACGCTGTCGAGC
GCGCGCATGCGCCTGCTGGCGCGTACCGACTGGGGCTTCGTGCGCCAGCATGCGCGGGACGGCCTGCGCATGCAGGTCAG
CGCCGGCGCCAATATCGCCGAGCGGCTGATGGCTGTCGGCGAGCGCCACTACGGCGACTTGCGCGAGATTGCCGGCGCGT
GGCTCGAGAAGATGGGAATCGACCTGGGCCGCCTGGATGATGTGCCGACCACGTTCTCGGGCGGCATGCAGCAGCGCCTG
CAGATCGCGCGCAACCTGGTCACGCATCCGCGGCTGGTCTTCATGGACGAGCCGACGGCATCGCTTGACGTCTCAGTGCA
GGCACGCCTGCTCGACTTGCTGCGAAGGCTGGTGACAGATCTCGATCTCGCCGCCATCCTGGTCACGCACGACCTCGCCG
TGGCCCGTTTGCTCGCGCACCGCACGCTGGTGATGCAAGGCGGCCGCGTGGTCGAGCAAGGCCTGACCGACCAGATTCTC
GACGATCCGCAGCATCCCTATACGCAGTTGCTGGTTTCGTCCATCCTGCAAGGCTGA

Upstream 100 bases:

>100_bases
AGATCATTACCGATGATGCCGGCACGCGCATGTTCGTGTGCTCCGACACGGAATACTGCGGCGACCGCGTTGCCGCGCAG
CGGGCCGAAGGAGCCGCACA

Downstream 100 bases:

>100_bases
CCATGACACAAGCCGCACCCAAGCAAACGATGATCGAGGTCCGTGGCCTCGGCAAGATGTTCACGCTGCACAACCAGGGC
GGCATCCGGCTGCCGGTCTT

Product: phosphonate C-P lyase system protein PhnK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSATPLLSVRNLTRTWDGVHGCHDVSFDLYPGEVLCVVGESGSGKSTLLHALSMQAPPQRGSVSYDMREAGLTDLATLSS
ARMRLLARTDWGFVRQHARDGLRMQVSAGANIAERLMAVGERHYGDLREIAGAWLEKMGIDLGRLDDVPTTFSGGMQQRL
QIARNLVTHPRLVFMDEPTASLDVSVQARLLDLLRRLVTDLDLAAILVTHDLAVARLLAHRTLVMQGGRVVEQGLTDQIL
DDPQHPYTQLLVSSILQG

Sequences:

>Translated_258_residues
MSATPLLSVRNLTRTWDGVHGCHDVSFDLYPGEVLCVVGESGSGKSTLLHALSMQAPPQRGSVSYDMREAGLTDLATLSS
ARMRLLARTDWGFVRQHARDGLRMQVSAGANIAERLMAVGERHYGDLREIAGAWLEKMGIDLGRLDDVPTTFSGGMQQRL
QIARNLVTHPRLVFMDEPTASLDVSVQARLLDLLRRLVTDLDLAAILVTHDLAVARLLAHRTLVMQGGRVVEQGLTDQIL
DDPQHPYTQLLVSSILQG
>Mature_257_residues
SATPLLSVRNLTRTWDGVHGCHDVSFDLYPGEVLCVVGESGSGKSTLLHALSMQAPPQRGSVSYDMREAGLTDLATLSSA
RMRLLARTDWGFVRQHARDGLRMQVSAGANIAERLMAVGERHYGDLREIAGAWLEKMGIDLGRLDDVPTTFSGGMQQRLQ
IARNLVTHPRLVFMDEPTASLDVSVQARLLDLLRRLVTDLDLAAILVTHDLAVARLLAHRTLVMQGGRVVEQGLTDQILD
DPQHPYTQLLVSSILQG

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates. Probably responsible for energy coupling to the transport system [H]

COG id: COG4107

COG function: function code P; ABC-type phosphonate transport system, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI21536376, Length=230, Percent_Identity=25.6521739130435, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI156105685, Length=235, Percent_Identity=30.2127659574468, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI157502203, Length=243, Percent_Identity=26.7489711934156, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157502201, Length=243, Percent_Identity=26.7489711934156, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI150417984, Length=245, Percent_Identity=27.7551020408163, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI42741659, Length=224, Percent_Identity=29.4642857142857, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI48994997, Length=253, Percent_Identity=66.403162055336, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI145693107, Length=262, Percent_Identity=35.1145038167939, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789962, Length=249, Percent_Identity=36.144578313253, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788506, Length=260, Percent_Identity=38.4615384615385, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1787500, Length=243, Percent_Identity=35.8024691358025, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789963, Length=233, Percent_Identity=35.6223175965665, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788225, Length=264, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787758, Length=244, Percent_Identity=33.6065573770492, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI48994883, Length=259, Percent_Identity=34.3629343629344, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87081791, Length=263, Percent_Identity=30.4182509505703, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87081834, Length=211, Percent_Identity=36.4928909952607, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787759, Length=259, Percent_Identity=31.6602316602317, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786872, Length=253, Percent_Identity=31.2252964426877, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1786398, Length=255, Percent_Identity=27.4509803921569, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789032, Length=235, Percent_Identity=29.7872340425532, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1789672, Length=254, Percent_Identity=26.7716535433071, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1787089, Length=231, Percent_Identity=32.4675324675325, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787548, Length=262, Percent_Identity=29.3893129770992, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787547, Length=257, Percent_Identity=28.7937743190661, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787105, Length=233, Percent_Identity=33.9055793991416, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1789864, Length=255, Percent_Identity=27.843137254902, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787029, Length=238, Percent_Identity=27.3109243697479, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788761, Length=239, Percent_Identity=29.2887029288703, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788644, Length=256, Percent_Identity=29.296875, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789890, Length=237, Percent_Identity=32.0675105485232, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789891, Length=241, Percent_Identity=29.4605809128631, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790525, Length=249, Percent_Identity=26.9076305220884, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787370, Length=261, Percent_Identity=26.4367816091954, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788210, Length=240, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1790467, Length=235, Percent_Identity=28.0851063829787, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786703, Length=227, Percent_Identity=33.920704845815, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786563, Length=230, Percent_Identity=30, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786345, Length=241, Percent_Identity=28.2157676348548, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790162, Length=256, Percent_Identity=27.34375, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789873, Length=206, Percent_Identity=29.126213592233, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081709, Length=243, Percent_Identity=24.2798353909465, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1786253, Length=227, Percent_Identity=28.1938325991189, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI87082267, Length=251, Percent_Identity=25.4980079681275, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787712, Length=254, Percent_Identity=26.3779527559055, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790190, Length=213, Percent_Identity=27.2300469483568, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790534, Length=223, Percent_Identity=29.5964125560538, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787792, Length=241, Percent_Identity=27.3858921161826, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788897, Length=236, Percent_Identity=28.3898305084746, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787112, Length=248, Percent_Identity=29.4354838709677, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787164, Length=205, Percent_Identity=32.1951219512195, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI48995001, Length=226, Percent_Identity=27.8761061946903, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786319, Length=237, Percent_Identity=26.5822784810127, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789991, Length=228, Percent_Identity=26.7543859649123, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789593, Length=243, Percent_Identity=27.5720164609054, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788450, Length=244, Percent_Identity=25, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87082268, Length=240, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790544, Length=246, Percent_Identity=26.4227642276423, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786981, Length=248, Percent_Identity=29.0322580645161, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788002, Length=234, Percent_Identity=27.3504273504274, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=235, Percent_Identity=26.8085106382979, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25146777, Length=221, Percent_Identity=28.5067873303167, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71984940, Length=221, Percent_Identity=27.1493212669683, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115534520, Length=230, Percent_Identity=25.2173913043478, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322640, Length=245, Percent_Identity=25.7142857142857, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641565, Length=225, Percent_Identity=29.3333333333333, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21357907, Length=226, Percent_Identity=26.1061946902655, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28574744, Length=170, Percent_Identity=31.1764705882353, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24659289, Length=216, Percent_Identity=27.7777777777778, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI85726357, Length=239, Percent_Identity=25.5230125523013, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28574533, Length=216, Percent_Identity=27.3148148148148, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI116007184, Length=234, Percent_Identity=23.0769230769231, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI221500365, Length=234, Percent_Identity=23.0769230769231, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- InterPro:   IPR012700
- InterPro:   IPR013563 [H]

Pfam domain/function: PF00005 ABC_tran; PF08352 oligo_HPY [H]

EC number: NA

Molecular weight: Translated: 28310; Mature: 28179

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATPLLSVRNLTRTWDGVHGCHDVSFDLYPGEVLCVVGESGSGKSTLLHALSMQAPPQR
CCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCC
GSVSYDMREAGLTDLATLSSARMRLLARTDWGFVRQHARDGLRMQVSAGANIAERLMAVG
CCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHH
ERHYGDLREIAGAWLEKMGIDLGRLDDVPTTFSGGMQQRLQIARNLVTHPRLVFMDEPTA
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCEEEEEECCCC
SLDVSVQARLLDLLRRLVTDLDLAAILVTHDLAVARLLAHRTLVMQGGRVVEQGLTDQIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHH
DDPQHPYTQLLVSSILQG
CCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SATPLLSVRNLTRTWDGVHGCHDVSFDLYPGEVLCVVGESGSGKSTLLHALSMQAPPQR
CCCCCHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCC
GSVSYDMREAGLTDLATLSSARMRLLARTDWGFVRQHARDGLRMQVSAGANIAERLMAVG
CCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHH
ERHYGDLREIAGAWLEKMGIDLGRLDDVPTTFSGGMQQRLQIARNLVTHPRLVFMDEPTA
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCEEEEEECCCC
SLDVSVQARLLDLLRRLVTDLDLAAILVTHDLAVARLLAHRTLVMQGGRVVEQGLTDQIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHH
DDPQHPYTQLLVSSILQG
CCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]