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The map label for this gene is phnI [H]

Identifier: 73538018

GI number: 73538018

Start: 829469

End: 830605

Strand: Direct

Name: phnI [H]

Synonym: Reut_B4188

Alternate gene names: 73538018

Gene position: 829469-830605 (Clockwise)

Preceding gene: 73538017

Following gene: 73538019

Centisome position: 30.43

GC content: 66.75

Gene sequence:

>1137_bases
ATGTACGTAGCCGTCAAGGGTGGCGAGCGCGCTATCCTCAATTCCTATCAGATGCTCGACGCCTATCGCCGCGGCGATAC
CGCGGTTCCTGAACTGACGCTGGCCCAGATCCGCGAACAAATGCCCCTGGCCGTCTCGCGCGTGATGGCGGAAGGCTCGC
TGTACGACCCGAACCTGGCCGCTCTGGCACTCAAGCAGGCTGCGGGGGACCAGATCGAAGCGATCTTCCTGCTGCGCGCC
TACCGCACCACGCTGCCGCGCTTTGGCTACACGGTGCCGGTCGATACCGGCAGGATGCGCCTGGAACGCCGGATCTCGTC
GACGTTCAAGGATGTCCCTGGCGGTCAGGTACTTGGCCCGACGTACGACTACACGCAGCGCCTGCTCGATTTCTCGCTCG
AAGCCGAGCGCGATCCGGCTCCGCTGGCGCCGTCGCCGGAGCCGCTGGCGTCCGACATGCCGCGCGTGACGGCGCTTCTC
GAAGCCGACGAACTGGTCGAGGCCGATGCCATTCCGCCGGGCGACCCGGCTCCCCCCGACCTGACCCGCGAACCGCCGAC
GCTGCCGGCCGGCCGCGCGGCACGCCTGCAGAACCTTGCGCGCGGGGACGAAGGTTTCCTGTTGTCGATGGGCTATTCCA
CGCAGCGCGGCTACGGCAACTCGCACCCGTTCGCGGCGGAAATCCGCTACGGCACTGCCGAAGTCGAGCTGTTCGTGGAG
GAACTGGGCTTTGCCATCACCATCGGCGAGGTCGAGCTGACCGAGTGCCAGATGGTGAGCCAGTTCACCGGCAACGCCAA
CGAGGCGCCGCGCTTCACGCGTGGCTACGGGCTGGTGTTCGGCTACGGCGAACGCAAGGCCATGTCGATGGCGCTGGCCG
ACCGCGCCATGCGTGCGGCAGAACTTGGCGAAGCGGCCGACGCCCCGGCCAATGACGTGGAATTCATGCTCTACCACAGC
GACAACGTCGAGGCCTCCGGCTTCGTGCAGCACCTGAAGCTGCCCCATTACGTGGACTTCCAGGCCAACCTGGAACTGCT
GCGCCGCCTGCGCACCGAGCAGGAAGCCGCGGGTACGGAAGCTGCTCCTGCCGCCGCGGTATCGAGTATCCAGCCACAAG
AGGAGACGCTGGCATGA

Upstream 100 bases:

>100_bases
CGCGACAACCATGCGGTGTTCCCGCTCGGCGTCGATCTGCTGCTGACCAGCGGCGAGCAATTCTGCGCCCTGGCGCGCAC
CACGCAGCTGGAGGACTGAG

Downstream 100 bases:

>100_bases
GTCAGACCAACGCCACCGTCGCCCGCGACGACCACTACAACTTTGGCTACCTGGACGAATCAACCAAGCGCATGCTGCGC
CGTGCGCTGCTGAAGGCCGT

Product: phosphonate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MYVAVKGGERAILNSYQMLDAYRRGDTAVPELTLAQIREQMPLAVSRVMAEGSLYDPNLAALALKQAAGDQIEAIFLLRA
YRTTLPRFGYTVPVDTGRMRLERRISSTFKDVPGGQVLGPTYDYTQRLLDFSLEAERDPAPLAPSPEPLASDMPRVTALL
EADELVEADAIPPGDPAPPDLTREPPTLPAGRAARLQNLARGDEGFLLSMGYSTQRGYGNSHPFAAEIRYGTAEVELFVE
ELGFAITIGEVELTECQMVSQFTGNANEAPRFTRGYGLVFGYGERKAMSMALADRAMRAAELGEAADAPANDVEFMLYHS
DNVEASGFVQHLKLPHYVDFQANLELLRRLRTEQEAAGTEAAPAAAVSSIQPQEETLA

Sequences:

>Translated_378_residues
MYVAVKGGERAILNSYQMLDAYRRGDTAVPELTLAQIREQMPLAVSRVMAEGSLYDPNLAALALKQAAGDQIEAIFLLRA
YRTTLPRFGYTVPVDTGRMRLERRISSTFKDVPGGQVLGPTYDYTQRLLDFSLEAERDPAPLAPSPEPLASDMPRVTALL
EADELVEADAIPPGDPAPPDLTREPPTLPAGRAARLQNLARGDEGFLLSMGYSTQRGYGNSHPFAAEIRYGTAEVELFVE
ELGFAITIGEVELTECQMVSQFTGNANEAPRFTRGYGLVFGYGERKAMSMALADRAMRAAELGEAADAPANDVEFMLYHS
DNVEASGFVQHLKLPHYVDFQANLELLRRLRTEQEAAGTEAAPAAAVSSIQPQEETLA
>Mature_378_residues
MYVAVKGGERAILNSYQMLDAYRRGDTAVPELTLAQIREQMPLAVSRVMAEGSLYDPNLAALALKQAAGDQIEAIFLLRA
YRTTLPRFGYTVPVDTGRMRLERRISSTFKDVPGGQVLGPTYDYTQRLLDFSLEAERDPAPLAPSPEPLASDMPRVTALL
EADELVEADAIPPGDPAPPDLTREPPTLPAGRAARLQNLARGDEGFLLSMGYSTQRGYGNSHPFAAEIRYGTAEVELFVE
ELGFAITIGEVELTECQMVSQFTGNANEAPRFTRGYGLVFGYGERKAMSMALADRAMRAAELGEAADAPANDVEFMLYHS
DNVEASGFVQHLKLPHYVDFQANLELLRRLRTEQEAAGTEAAPAAAVSSIQPQEETLA

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3626

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnI family [H]

Homologues:

Organism=Escherichia coli, GI1790537, Length=354, Percent_Identity=58.7570621468927, Blast_Score=410, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008773 [H]

Pfam domain/function: PF05861 PhnI [H]

EC number: NA

Molecular weight: Translated: 41248; Mature: 41248

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVAVKGGERAILNSYQMLDAYRRGDTAVPELTLAQIREQMPLAVSRVMAEGSLYDPNLA
CEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCHH
ALALKQAAGDQIEAIFLLRAYRTTLPRFGYTVPVDTGRMRLERRISSTFKDVPGGQVLGP
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCEECC
TYDYTQRLLDFSLEAERDPAPLAPSPEPLASDMPRVTALLEADELVEADAIPPGDPAPPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LTREPPTLPAGRAARLQNLARGDEGFLLSMGYSTQRGYGNSHPFAAEIRYGTAEVELFVE
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHH
ELGFAITIGEVELTECQMVSQFTGNANEAPRFTRGYGLVFGYGERKAMSMALADRAMRAA
HCCCEEEECCEEHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHH
ELGEAADAPANDVEFMLYHSDNVEASGFVQHLKLPHYVDFQANLELLRRLRTEQEAAGTE
HHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHCCCC
AAPAAAVSSIQPQEETLA
CCCHHHHHCCCCHHHHCC
>Mature Secondary Structure
MYVAVKGGERAILNSYQMLDAYRRGDTAVPELTLAQIREQMPLAVSRVMAEGSLYDPNLA
CEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCHH
ALALKQAAGDQIEAIFLLRAYRTTLPRFGYTVPVDTGRMRLERRISSTFKDVPGGQVLGP
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCEECC
TYDYTQRLLDFSLEAERDPAPLAPSPEPLASDMPRVTALLEADELVEADAIPPGDPAPPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LTREPPTLPAGRAARLQNLARGDEGFLLSMGYSTQRGYGNSHPFAAEIRYGTAEVELFVE
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHH
ELGFAITIGEVELTECQMVSQFTGNANEAPRFTRGYGLVFGYGERKAMSMALADRAMRAA
HCCCEEEECCEEHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHH
ELGEAADAPANDVEFMLYHSDNVEASGFVQHLKLPHYVDFQANLELLRRLRTEQEAAGTE
HHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHCCCC
AAPAAAVSSIQPQEETLA
CCCHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]