Click here to switch to the map view.

The map label for this gene is rspA [H]

Identifier: 73537953

GI number: 73537953

Start: 761424

End: 762614

Strand: Reverse

Name: rspA [H]

Synonym: Reut_B4122

Alternate gene names: 73537953

Gene position: 762614-761424 (Counterclockwise)

Preceding gene: 73537956

Following gene: 73537952

Centisome position: 27.97

GC content: 59.87

Gene sequence:

>1191_bases
ATGCGTATTGAGCACTTTGAGACCATTTCCTGCGATGCAGGGTGGCGAAACTACTACTTCCTGAAAGTGGTGACATCGGA
CGGAATCGTCGGCTGGAGCGAATATGACGAATCGTTCGGGCCGCCGGGTCTGACCGCTGTCATCGAGCAACTAGGGGCGC
GGTTGGTCGGGAAATCGGTGTATGACCACGAAGAGGCATATGGCCGGCTTGCGGCATCCCTGCGCCCCGCTCCCTACGGG
CTCAGCGCCGAGGCCATTGGGGCAATCGAGAATGCGCTGCTCGACGCGAAAGCCAAATCGCTGGGGTTACCGGTCTATCA
ATTGCTGGGTGGCAAGCACCGGGGCAGCATCCCCATCTATTGGTCTCACTGCGCAACCTGGCGAATCAGCCACCCAAACC
TCTACCCACCGGCGATCGTCGATCTGGACGGCGTCAAGAAGGCTGGCGAGGACGCACGCAAATCCGGGATTTCCGCTCTC
AAGACCAATCTCTTTATCAATGAAAACGGCAAGCCGCGACAGTGGATGGCCGGCTTCGGCTCGCCCTACGCGCCGTCGCT
CAATGTGGACAAGGCATTGCTGCGCAATCTGCGTGCGCACCTCGAAGCGCTGCGCGATGGTGCGGGTCCGGACGTCGACA
TCCTGGTGGACCTGAATTTCAACGCGCGTACAGAGGGCTACCTCTCGCTCATCAACGCCATCAAGGACTTCGACCTCTTC
TGGGTAGAACTCGATATCTACAATCCGGACGCCATGGCGTACATCCGCCAACGGAGCCACGCACCGATTGCAGCGTGCGA
AACGCTCTTTGGCATCCGCCAGTTCCTGCCCTACTTCCAGGCGCAGTCAATGGATGTGGCCATCGTCGACGCAGTCTGGA
ATGGTGTGTGGCAAGCCATGAAGATCGCCAGCGTGGCAGACGCCTTTGACGTCAACATCGCCCCGCACAACTTCTATAGC
CACCTGTCGACCATGATGAACGTCCATTTCGCGGCGGCGGTTCCAAACCTGCGCGTGATGGAAGTTGACGTCGATCGTCT
GGCCTGGGACGACCAGTTGTTCACCCGTACGCCTGAGATCGTGAATGGTGCCATTCAAGTTCCGGACGCGCCGGGATGGG
GCTGCGACCCCATCGAAGAGGCCCTGCGCGCCCATCCGCCGCAGGTTCGTCGCAGCCATCTTGGTCTGTGA

Upstream 100 bases:

>100_bases
ACCTCGGGTTTACCTTAGTTGCCTCCCGGCATTGCTCCATCTAGGCTTCGTGTTAATTGTTAACAATTATTTGTTGTTTG
TTGTCACGGACGAAAGAACC

Downstream 100 bases:

>100_bases
GCCCAACCGCTGGCGCACGGGGAATCGATGCCAAGGGCGGGAATGCTTTTCCCCGTCGCGCCTGATTGCCACCTACTACT
TCCAGTCAGCTTTCGAGGCG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MRIEHFETISCDAGWRNYYFLKVVTSDGIVGWSEYDESFGPPGLTAVIEQLGARLVGKSVYDHEEAYGRLAASLRPAPYG
LSAEAIGAIENALLDAKAKSLGLPVYQLLGGKHRGSIPIYWSHCATWRISHPNLYPPAIVDLDGVKKAGEDARKSGISAL
KTNLFINENGKPRQWMAGFGSPYAPSLNVDKALLRNLRAHLEALRDGAGPDVDILVDLNFNARTEGYLSLINAIKDFDLF
WVELDIYNPDAMAYIRQRSHAPIAACETLFGIRQFLPYFQAQSMDVAIVDAVWNGVWQAMKIASVADAFDVNIAPHNFYS
HLSTMMNVHFAAAVPNLRVMEVDVDRLAWDDQLFTRTPEIVNGAIQVPDAPGWGCDPIEEALRAHPPQVRRSHLGL

Sequences:

>Translated_396_residues
MRIEHFETISCDAGWRNYYFLKVVTSDGIVGWSEYDESFGPPGLTAVIEQLGARLVGKSVYDHEEAYGRLAASLRPAPYG
LSAEAIGAIENALLDAKAKSLGLPVYQLLGGKHRGSIPIYWSHCATWRISHPNLYPPAIVDLDGVKKAGEDARKSGISAL
KTNLFINENGKPRQWMAGFGSPYAPSLNVDKALLRNLRAHLEALRDGAGPDVDILVDLNFNARTEGYLSLINAIKDFDLF
WVELDIYNPDAMAYIRQRSHAPIAACETLFGIRQFLPYFQAQSMDVAIVDAVWNGVWQAMKIASVADAFDVNIAPHNFYS
HLSTMMNVHFAAAVPNLRVMEVDVDRLAWDDQLFTRTPEIVNGAIQVPDAPGWGCDPIEEALRAHPPQVRRSHLGL
>Mature_396_residues
MRIEHFETISCDAGWRNYYFLKVVTSDGIVGWSEYDESFGPPGLTAVIEQLGARLVGKSVYDHEEAYGRLAASLRPAPYG
LSAEAIGAIENALLDAKAKSLGLPVYQLLGGKHRGSIPIYWSHCATWRISHPNLYPPAIVDLDGVKKAGEDARKSGISAL
KTNLFINENGKPRQWMAGFGSPYAPSLNVDKALLRNLRAHLEALRDGAGPDVDILVDLNFNARTEGYLSLINAIKDFDLF
WVELDIYNPDAMAYIRQRSHAPIAACETLFGIRQFLPYFQAQSMDVAIVDAVWNGVWQAMKIASVADAFDVNIAPHNFYS
HLSTMMNVHFAAAVPNLRVMEVDVDRLAWDDQLFTRTPEIVNGAIQVPDAPGWGCDPIEEALRAHPPQVRRSHLGL

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=392, Percent_Identity=25, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 43828; Mature: 43828

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEHFETISCDAGWRNYYFLKVVTSDGIVGWSEYDESFGPPGLTAVIEQLGARLVGKSV
CCCCCCEEEECCCCCCEEEEEEEEECCCEECHHHHHHCCCCCCHHHHHHHHHHHHHHCHH
YDHEEAYGRLAASLRPAPYGLSAEAIGAIENALLDAKAKSLGLPVYQLLGGKHRGSIPIY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEE
WSHCATWRISHPNLYPPAIVDLDGVKKAGEDARKSGISALKTNLFINENGKPRQWMAGFG
ECCCEEEEECCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHEEEEECCCCCCHHHHHCCC
SPYAPSLNVDKALLRNLRAHLEALRDGAGPDVDILVDLNFNARTEGYLSLINAIKDFDLF
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCEEE
WVELDIYNPDAMAYIRQRSHAPIAACETLFGIRQFLPYFQAQSMDVAIVDAVWNGVWQAM
EEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHH
KIASVADAFDVNIAPHNFYSHLSTMMNVHFAAAVPNLRVMEVDVDRLAWDDQLFTRTPEI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCHHHHCCHHH
VNGAIQVPDAPGWGCDPIEEALRAHPPQVRRSHLGL
HCCEEECCCCCCCCCCHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MRIEHFETISCDAGWRNYYFLKVVTSDGIVGWSEYDESFGPPGLTAVIEQLGARLVGKSV
CCCCCCEEEECCCCCCEEEEEEEEECCCEECHHHHHHCCCCCCHHHHHHHHHHHHHHCHH
YDHEEAYGRLAASLRPAPYGLSAEAIGAIENALLDAKAKSLGLPVYQLLGGKHRGSIPIY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEE
WSHCATWRISHPNLYPPAIVDLDGVKKAGEDARKSGISALKTNLFINENGKPRQWMAGFG
ECCCEEEEECCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHEEEEECCCCCCHHHHHCCC
SPYAPSLNVDKALLRNLRAHLEALRDGAGPDVDILVDLNFNARTEGYLSLINAIKDFDLF
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCEEE
WVELDIYNPDAMAYIRQRSHAPIAACETLFGIRQFLPYFQAQSMDVAIVDAVWNGVWQAM
EEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHH
KIASVADAFDVNIAPHNFYSHLSTMMNVHFAAAVPNLRVMEVDVDRLAWDDQLFTRTPEI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCHHHHCCHHH
VNGAIQVPDAPGWGCDPIEEALRAHPPQVRRSHLGL
HCCEEECCCCCCCCCCHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]