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The map label for this gene is yafC [H]

Identifier: 73537956

GI number: 73537956

Start: 764519

End: 765430

Strand: Reverse

Name: yafC [H]

Synonym: Reut_B4125

Alternate gene names: 73537956

Gene position: 765430-764519 (Counterclockwise)

Preceding gene: 73537962

Following gene: 73537953

Centisome position: 28.08

GC content: 61.4

Gene sequence:

>912_bases
ATGAAACGGCAATTCGATGACCTCCAACTAGGCAGCATCGAGCTGTTCTGCCTTGCCGCCGAACTCGGAAGCTTCACCGC
CGCCGCGGTGGCCGCGAGCGTGACGCCAGCAGCGGTCAGCCGCACCGTGCAAAGGCTGGAAGAACGCCTCGGCGCGCGCC
TCTTCGTGCGAACCACACGTCAGATTCGCCTGACCGATGAAGGACGGGTCTATTTCGAACAGAGCCGTGCCGCATTGGCG
CAGTTGGTTGACGCGGAACGGCAGATCAGCGGCCAGCAAGCCGCGCCGGCAGGACGGCTTCGTATCAGCCTGCCAACGCC
TTATGCACACTATCGCGTGCTGCCTGTGTTGCCTGAATTTCGGTCGCTGTACCCTGAAGTCGAAATCGACGTGCACATCA
GCAACCGCAACGTGGACTTTGCCGATGACACCTACGACCTCTCCGTGCGCGGGCGCGCACCGGACGATTCGAATCTGATC
GCACGCAAACTGGAAGATGCGGAGCTGGTTGTCGTGGCGACGCCCGAATATCTGCGGCGCGCCGGCAAGCCAGAGTCGCC
GGACGACCTGCCCAGGCATGAGTGCATTCAGTTCGAACGCCCCAGCAGCGGCCGCAGGATCCCATGGACGTTCCAGGTCA
ACGACGAGGACACGGATATCATGACGGCCGGCTCTTACGCAGCCTCCGAAGATATTCTCGGTGGCGCGACATTGGTGCGT
GCCGGCGCAGGCCTGTTCCAGACTTACCGTTTTGTCGTGGAACAGGATTTGCGGGACGGCACGCTCGTGGAAGTGCTGCA
AGAATTTGGCGGCAGTACCCGGCCTTTCGTGCTGCTCTACCCTTCCGCACGCTACGTCACACGCCGGGTCCGCGCATTCG
TCGATTTCCTGGTCGATAAATTCTCAGCATAA

Upstream 100 bases:

>100_bases
TCTCGCTTGCGGTGGCACGACAACATCCAGTCGAGCATTACGGATGTCGCTACAGCCACCGGCCCGCCATTACCAAGATG
CCTCCGACAGAAACCAGAAC

Downstream 100 bases:

>100_bases
CGGCATGCCCTCACGACCGGTCGCCGAAACCTCAATTGTAGAATGCAGGAATTAGCTTAAGCTGATCGGATTGTTTCTTG
TCATGATTGATGAAGAGCGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKRQFDDLQLGSIELFCLAAELGSFTAAAVAASVTPAAVSRTVQRLEERLGARLFVRTTRQIRLTDEGRVYFEQSRAALA
QLVDAERQISGQQAAPAGRLRISLPTPYAHYRVLPVLPEFRSLYPEVEIDVHISNRNVDFADDTYDLSVRGRAPDDSNLI
ARKLEDAELVVVATPEYLRRAGKPESPDDLPRHECIQFERPSSGRRIPWTFQVNDEDTDIMTAGSYAASEDILGGATLVR
AGAGLFQTYRFVVEQDLRDGTLVEVLQEFGGSTRPFVLLYPSARYVTRRVRAFVDFLVDKFSA

Sequences:

>Translated_303_residues
MKRQFDDLQLGSIELFCLAAELGSFTAAAVAASVTPAAVSRTVQRLEERLGARLFVRTTRQIRLTDEGRVYFEQSRAALA
QLVDAERQISGQQAAPAGRLRISLPTPYAHYRVLPVLPEFRSLYPEVEIDVHISNRNVDFADDTYDLSVRGRAPDDSNLI
ARKLEDAELVVVATPEYLRRAGKPESPDDLPRHECIQFERPSSGRRIPWTFQVNDEDTDIMTAGSYAASEDILGGATLVR
AGAGLFQTYRFVVEQDLRDGTLVEVLQEFGGSTRPFVLLYPSARYVTRRVRAFVDFLVDKFSA
>Mature_303_residues
MKRQFDDLQLGSIELFCLAAELGSFTAAAVAASVTPAAVSRTVQRLEERLGARLFVRTTRQIRLTDEGRVYFEQSRAALA
QLVDAERQISGQQAAPAGRLRISLPTPYAHYRVLPVLPEFRSLYPEVEIDVHISNRNVDFADDTYDLSVRGRAPDDSNLI
ARKLEDAELVVVATPEYLRRAGKPESPDDLPRHECIQFERPSSGRRIPWTFQVNDEDTDIMTAGSYAASEDILGGATLVR
AGAGLFQTYRFVVEQDLRDGTLVEVLQEFGGSTRPFVLLYPSARYVTRRVRAFVDFLVDKFSA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=33.5664335664336, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=34.1216216216216, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=28.5223367697594, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=28.2828282828283, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1789440, Length=280, Percent_Identity=28.9285714285714, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787589, Length=299, Percent_Identity=28.4280936454849, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=27.2108843537415, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=27.4247491638796, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788706, Length=179, Percent_Identity=31.2849162011173, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786448, Length=186, Percent_Identity=28.494623655914, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI157672245, Length=119, Percent_Identity=36.9747899159664, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790011, Length=298, Percent_Identity=24.8322147651007, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788481, Length=294, Percent_Identity=27.2108843537415, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082132, Length=300, Percent_Identity=23.6666666666667, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787601, Length=181, Percent_Identity=23.2044198895028, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693105, Length=124, Percent_Identity=29.0322580645161, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33904; Mature: 33904

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQFDDLQLGSIELFCLAAELGSFTAAAVAASVTPAAVSRTVQRLEERLGARLFVRTTR
CCCCCCCCCCCCEEEEEEEEHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEECE
QIRLTDEGRVYFEQSRAALAQLVDAERQISGQQAAPAGRLRISLPTPYAHYRVLPVLPEF
EEEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHEEEEECCHHH
RSLYPEVEIDVHISNRNVDFADDTYDLSVRGRAPDDSNLIARKLEDAELVVVATPEYLRR
HHHCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCEEEEECHHHHHH
AGKPESPDDLPRHECIQFERPSSGRRIPWTFQVNDEDTDIMTAGSYAASEDILGGATLVR
CCCCCCCCCCCHHHHEECCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCHHCCCHHHHH
AGAGLFQTYRFVVEQDLRDGTLVEVLQEFGGSTRPFVLLYPSARYVTRRVRAFVDFLVDK
HCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
FSA
HCC
>Mature Secondary Structure
MKRQFDDLQLGSIELFCLAAELGSFTAAAVAASVTPAAVSRTVQRLEERLGARLFVRTTR
CCCCCCCCCCCCEEEEEEEEHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEECE
QIRLTDEGRVYFEQSRAALAQLVDAERQISGQQAAPAGRLRISLPTPYAHYRVLPVLPEF
EEEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHEEEEECCHHH
RSLYPEVEIDVHISNRNVDFADDTYDLSVRGRAPDDSNLIARKLEDAELVVVATPEYLRR
HHHCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCEEEEECHHHHHH
AGKPESPDDLPRHECIQFERPSSGRRIPWTFQVNDEDTDIMTAGSYAASEDILGGATLVR
CCCCCCCCCCCHHHHEECCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCHHCCCHHHHH
AGAGLFQTYRFVVEQDLRDGTLVEVLQEFGGSTRPFVLLYPSARYVTRRVRAFVDFLVDK
HCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
FSA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]