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The map label for this gene is mdtG [H]

Identifier: 73537949

GI number: 73537949

Start: 757160

End: 758410

Strand: Reverse

Name: mdtG [H]

Synonym: Reut_B4118

Alternate gene names: 73537949

Gene position: 758410-757160 (Counterclockwise)

Preceding gene: 73537950

Following gene: 73537946

Centisome position: 27.82

GC content: 63.39

Gene sequence:

>1251_bases
ATGCCGAATCCCATCATCACCATCTCCGCCCGTGAAAGCCACTCGGGGGCCAACTACGCGGACCGCAACGCCTCGTACTG
GAAGCGCAACCTGGCCGTGGCGACTTTCGGGTCATTTACCACGCTGGTCAGTCTCAGCATGCTGTTGCCGTTCCTGCCAC
TCTATGTGGAGCAGCTTGGCATCACGGCGCAGAACGAGATCGTCCAATGGTCCGGCGTCGCCTTTGGCGTGACCTTTCTG
GCAACGGCCGTGACCGCGCCGCTATGGGGGCGACTGGCCGATCGCTTTGGGCGCAAGCCCATGCTGGTTCGCGCGGCGGT
GGGCATGGCCATCGTCATGTCGATGATCGGCCTGGCGCATAACATCTACGAACTCGTCGCCCTGCGTTTTGCGGCAGGCC
TCATCGGCGGCTATGCTTCGGCGGCCACCGTCATGATTGGCACGCAGGCGCCGGCCGAACGCGCGGGATGGGCGCTAGGC
GTGCTGTCCAGTGGCGCACTGGTGGGCAATCTGGTTGGTCCGCTTGTCGGCGGCCTGCTGCCGAACCTGATCGGCATCCG
CGGCACCTTTTTCGCGGGTGGCGCGATGATCTCGGTAGCCGCGCTGCTGACCATATTTCTGGTACGCGAGGACTTCAGGC
GACCGGCGAAAAGGACATTGTCCCATGAGGCTATAGCGGATAGGCACGAGCGCAGTTCGCGCGTTCCGCTGGTCGCGTTG
CTCGTTACAGCGACCATGGTGCTGTTTTCCAACATGTCGATCGAGCCAATTATCACCGTCTACATCGGTCAACTCGGCGT
ACCCCGTGCGCAACTATCGACCATTGCCGGCATCGTCATGGCGAGCTCGGCGCTCGGCAGCGTCTTGACGGCTTCCCGGC
TCGGCGCGCTGGCTGACCGCCTCGGGGCCTGGCGCGTGATCATCGGCTGCCTGGTCGCCACTGCTGTCGTCATGATTCCG
CAGGCGTTCGTCACAAACTGGTGGACTCTCGCTATTTTGCGTGGCCTCATGGGGATGACGGTAGCTGGATTGCTGCCAGC
GATCGGCAAGCTTGTGCGGCAGGCAACAAAGGAACACCAGTCAGGCAAGATGCTCGGCTACCTGCAGTCGGCACAGTTTG
CGGGCCAGGTGACCGGTCCGCTGATCGGTGGCCAGATCGGCGTGCATGTCGGCATGCGCGCGGTGTTCTTCGTCACCAGT
GCGGTGCTGCTGGGCTGCGCCGCTTTCGACCGCTGGGCGAAAACGCGGTGA

Upstream 100 bases:

>100_bases
GTCCGCCTTGCTCTGGCGGCAATCGGAGGATGGCGCGCTGATCTTGTGGCATGTCCGACCAGGACGTGATCTCGGTCCAC
CGATCGCGAGCTTTCTTGCC

Downstream 100 bases:

>100_bases
CTGAGCCCAGGTGGCGTTGTGCAATCCGAACGCGGGCGCTTCAACTTCGCCGGCCGTGATCAGCCAGCGCACATCGCCAG
CGCGTAGTACGACGCGCCGT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MPNPIITISARESHSGANYADRNASYWKRNLAVATFGSFTTLVSLSMLLPFLPLYVEQLGITAQNEIVQWSGVAFGVTFL
ATAVTAPLWGRLADRFGRKPMLVRAAVGMAIVMSMIGLAHNIYELVALRFAAGLIGGYASAATVMIGTQAPAERAGWALG
VLSSGALVGNLVGPLVGGLLPNLIGIRGTFFAGGAMISVAALLTIFLVREDFRRPAKRTLSHEAIADRHERSSRVPLVAL
LVTATMVLFSNMSIEPIITVYIGQLGVPRAQLSTIAGIVMASSALGSVLTASRLGALADRLGAWRVIIGCLVATAVVMIP
QAFVTNWWTLAILRGLMGMTVAGLLPAIGKLVRQATKEHQSGKMLGYLQSAQFAGQVTGPLIGGQIGVHVGMRAVFFVTS
AVLLGCAAFDRWAKTR

Sequences:

>Translated_416_residues
MPNPIITISARESHSGANYADRNASYWKRNLAVATFGSFTTLVSLSMLLPFLPLYVEQLGITAQNEIVQWSGVAFGVTFL
ATAVTAPLWGRLADRFGRKPMLVRAAVGMAIVMSMIGLAHNIYELVALRFAAGLIGGYASAATVMIGTQAPAERAGWALG
VLSSGALVGNLVGPLVGGLLPNLIGIRGTFFAGGAMISVAALLTIFLVREDFRRPAKRTLSHEAIADRHERSSRVPLVAL
LVTATMVLFSNMSIEPIITVYIGQLGVPRAQLSTIAGIVMASSALGSVLTASRLGALADRLGAWRVIIGCLVATAVVMIP
QAFVTNWWTLAILRGLMGMTVAGLLPAIGKLVRQATKEHQSGKMLGYLQSAQFAGQVTGPLIGGQIGVHVGMRAVFFVTS
AVLLGCAAFDRWAKTR
>Mature_415_residues
PNPIITISARESHSGANYADRNASYWKRNLAVATFGSFTTLVSLSMLLPFLPLYVEQLGITAQNEIVQWSGVAFGVTFLA
TAVTAPLWGRLADRFGRKPMLVRAAVGMAIVMSMIGLAHNIYELVALRFAAGLIGGYASAATVMIGTQAPAERAGWALGV
LSSGALVGNLVGPLVGGLLPNLIGIRGTFFAGGAMISVAALLTIFLVREDFRRPAKRTLSHEAIADRHERSSRVPLVALL
VTATMVLFSNMSIEPIITVYIGQLGVPRAQLSTIAGIVMASSALGSVLTASRLGALADRLGAWRVIIGCLVATAVVMIPQ
AFVTNWWTLAILRGLMGMTVAGLLPAIGKLVRQATKEHQSGKMLGYLQSAQFAGQVTGPLIGGQIGVHVGMRAVFFVTSA
VLLGCAAFDRWAKTR

Specific function: Confers resistance to fosfomycin and deoxycholate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtG (TC 2.A.1.2.20) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=390, Percent_Identity=39.4871794871795, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI87082404, Length=355, Percent_Identity=25.9154929577465, Blast_Score=82, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6319871, Length=153, Percent_Identity=28.1045751633987, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43913; Mature: 43781

Theoretical pI: Translated: 11.38; Mature: 11.38

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNPIITISARESHSGANYADRNASYWKRNLAVATFGSFTTLVSLSMLLPFLPLYVEQLG
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ITAQNEIVQWSGVAFGVTFLATAVTAPLWGRLADRFGRKPMLVRAAVGMAIVMSMIGLAH
CCHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
NIYELVALRFAAGLIGGYASAATVMIGTQAPAERAGWALGVLSSGALVGNLVGPLVGGLL
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCEEHHHHHCCHHHHHHHHHHHHHHH
PNLIGIRGTFFAGGAMISVAALLTIFLVREDFRRPAKRTLSHEAIADRHERSSRVPLVAL
HHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LVTATMVLFSNMSIEPIITVYIGQLGVPRAQLSTIAGIVMASSALGSVLTASRLGALADR
HHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAWRVIIGCLVATAVVMIPQAFVTNWWTLAILRGLMGMTVAGLLPAIGKLVRQATKEHQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGKMLGYLQSAQFAGQVTGPLIGGQIGVHVGMRAVFFVTSAVLLGCAAFDRWAKTR
CCCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PNPIITISARESHSGANYADRNASYWKRNLAVATFGSFTTLVSLSMLLPFLPLYVEQLG
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ITAQNEIVQWSGVAFGVTFLATAVTAPLWGRLADRFGRKPMLVRAAVGMAIVMSMIGLAH
CCHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
NIYELVALRFAAGLIGGYASAATVMIGTQAPAERAGWALGVLSSGALVGNLVGPLVGGLL
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCEEHHHHHCCHHHHHHHHHHHHHHH
PNLIGIRGTFFAGGAMISVAALLTIFLVREDFRRPAKRTLSHEAIADRHERSSRVPLVAL
HHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LVTATMVLFSNMSIEPIITVYIGQLGVPRAQLSTIAGIVMASSALGSVLTASRLGALADR
HHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAWRVIIGCLVATAVVMIPQAFVTNWWTLAILRGLMGMTVAGLLPAIGKLVRQATKEHQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGKMLGYLQSAQFAGQVTGPLIGGQIGVHVGMRAVFFVTSAVLLGCAAFDRWAKTR
CCCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA