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The map label for this gene is dmlA [H]

Identifier: 73537946

GI number: 73537946

Start: 755204

End: 756367

Strand: Reverse

Name: dmlA [H]

Synonym: Reut_B4115

Alternate gene names: 73537946

Gene position: 756367-755204 (Counterclockwise)

Preceding gene: 73537949

Following gene: 73537945

Centisome position: 27.74

GC content: 65.64

Gene sequence:

>1164_bases
ATGATCCGCGCGCGGTTTACCGGCATCCTCGATCGCGTCCAGACCACCAGACACACCAGACCCGAGCGAGACACCGCCAT
GACCAAACCATCTCCAGCTTCGTTCCGGATTGCCGCCATTGCCGGCGACGGCATCGGCAACGAAGTCCTCCCCGAAGGGC
TGCGCGTCGTCGAAGCCGCCGCGCGCAAGTTCGACCTGCTCATTGAAGTGCGCCACTTCGAATGGGCCAACTGCGACTAT
TACCTGCGCCACGGCAAGATGATGCCGGACGACTGGAAGCAGCAGCTCGACGGCTTCGACGCCATCTACTTCGGCGCCGT
CGGCTGGCCCGACAAGGTACCCGACCACATCTCGCTGTGGGGCTCTCTGCTCAAGTTCCGCCGCGAATTCGACCAGTACG
TTAACCTGCGCCCCGTGCGCCTGCTGCCTGGCGTGCCCTGCCCGCTGGCCGGCAAGAAGCCCGGCGACATCGACTTCTAC
GTGGTCCGCGAGAACACCGAGGGCGAGTACAGTTCCGTCGGCGGCCGCATGTACGAAGGCACCGAGCGCGAGCTGGTGAT
GCAGCAGTCGATCTTCAGCCGCCACGGCACCGACCGCATCCTGAAGTACGCGTTCGAACTCGCGCAATCCCGTCCCCGCA
AAAAGCTTACGTCGGCGACCAAGTCCAACGGCATTGCCGTCAGCATGCCGTGGTGGGACGAGCGCACCGCCGCGATGGGG
GCGCAGTACCCGGAGATTGAATGGGACAGCCAGCACATCGACATCCTGTGCGCCCGCTTCGTGCTGCAGCCCGAGCGCTT
TGACGTGGTGGTGGCCTCCAACCTGTTCGGCGACCTGCTCTCCGACCTCGGCCCGGCGTGCACCGGCACCATCGGCCTGG
CAGGTTCGGCGAACCTGAACCCGGAACGCAAGTTCCCGTCGCTGTTCGAACCAGTGCACGGCTCGGCGCCGGACATCTTC
GGCAAGCAGATCGCCAACCCCATCGGCATGATCTGGTCGGGCGCGATGATGCTGGACTTCCTGGGCGGTGAAGCCGGACG
CCAGGCACATGACGCGATCCTCGCCGCGATCGAGACCGTGTTGCGCGAAGGTCCGCTGACGCCGGATGCCGGCGGCAAGG
CCGGCACGAGCGACGTGGGCAAGGCCATCGCCGAAGCGGTCTGA

Upstream 100 bases:

>100_bases
ACGAAGCTGGCGCGCTGCGCGACCAGGCAGAACACCCGCAGGTCTTCCAGTGGGGGCAGATTATTCACGATTCGTGTTTC
CTGTATCCACGCTTGCGCGG

Downstream 100 bases:

>100_bases
GGCCGCTCGCGCCGCGGCGTGACGCACGATTCGCCTCGCGAGTCGTTTCCCGTGGCGCACGACCATCCCTTCGTCACATA
AGTAATTTTTTTCCGCGAGG

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MIRARFTGILDRVQTTRHTRPERDTAMTKPSPASFRIAAIAGDGIGNEVLPEGLRVVEAAARKFDLLIEVRHFEWANCDY
YLRHGKMMPDDWKQQLDGFDAIYFGAVGWPDKVPDHISLWGSLLKFRREFDQYVNLRPVRLLPGVPCPLAGKKPGDIDFY
VVRENTEGEYSSVGGRMYEGTERELVMQQSIFSRHGTDRILKYAFELAQSRPRKKLTSATKSNGIAVSMPWWDERTAAMG
AQYPEIEWDSQHIDILCARFVLQPERFDVVVASNLFGDLLSDLGPACTGTIGLAGSANLNPERKFPSLFEPVHGSAPDIF
GKQIANPIGMIWSGAMMLDFLGGEAGRQAHDAILAAIETVLREGPLTPDAGGKAGTSDVGKAIAEAV

Sequences:

>Translated_387_residues
MIRARFTGILDRVQTTRHTRPERDTAMTKPSPASFRIAAIAGDGIGNEVLPEGLRVVEAAARKFDLLIEVRHFEWANCDY
YLRHGKMMPDDWKQQLDGFDAIYFGAVGWPDKVPDHISLWGSLLKFRREFDQYVNLRPVRLLPGVPCPLAGKKPGDIDFY
VVRENTEGEYSSVGGRMYEGTERELVMQQSIFSRHGTDRILKYAFELAQSRPRKKLTSATKSNGIAVSMPWWDERTAAMG
AQYPEIEWDSQHIDILCARFVLQPERFDVVVASNLFGDLLSDLGPACTGTIGLAGSANLNPERKFPSLFEPVHGSAPDIF
GKQIANPIGMIWSGAMMLDFLGGEAGRQAHDAILAAIETVLREGPLTPDAGGKAGTSDVGKAIAEAV
>Mature_387_residues
MIRARFTGILDRVQTTRHTRPERDTAMTKPSPASFRIAAIAGDGIGNEVLPEGLRVVEAAARKFDLLIEVRHFEWANCDY
YLRHGKMMPDDWKQQLDGFDAIYFGAVGWPDKVPDHISLWGSLLKFRREFDQYVNLRPVRLLPGVPCPLAGKKPGDIDFY
VVRENTEGEYSSVGGRMYEGTERELVMQQSIFSRHGTDRILKYAFELAQSRPRKKLTSATKSNGIAVSMPWWDERTAAMG
AQYPEIEWDSQHIDILCARFVLQPERFDVVVASNLFGDLLSDLGPACTGTIGLAGSANLNPERKFPSLFEPVHGSAPDIF
GKQIANPIGMIWSGAMMLDFLGGEAGRQAHDAILAAIETVLREGPLTPDAGGKAGTSDVGKAIAEAV

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=358, Percent_Identity=30.1675977653631, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4758582, Length=369, Percent_Identity=29.5392953929539, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI28178838, Length=350, Percent_Identity=28.8571428571429, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI28178816, Length=364, Percent_Identity=25.8241758241758, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI28178821, Length=360, Percent_Identity=25.8333333333333, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI28178819, Length=209, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=75.2808988764045, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=36.0881542699724, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1787381, Length=382, Percent_Identity=27.2251308900524, Blast_Score=108, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17550882, Length=369, Percent_Identity=28.4552845528455, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71986051, Length=258, Percent_Identity=32.5581395348837, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=359, Percent_Identity=25.9052924791086, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=266, Percent_Identity=29.6992481203008, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322097, Length=369, Percent_Identity=36.5853658536585, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6324709, Length=370, Percent_Identity=29.4594594594595, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6319830, Length=367, Percent_Identity=31.0626702997275, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6324291, Length=386, Percent_Identity=27.979274611399, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24643268, Length=361, Percent_Identity=32.6869806094183, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24643270, Length=361, Percent_Identity=32.6869806094183, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24661184, Length=354, Percent_Identity=31.0734463276836, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI161078635, Length=266, Percent_Identity=31.5789473684211, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI161078637, Length=266, Percent_Identity=31.5789473684211, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI161078633, Length=266, Percent_Identity=31.5789473684211, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24650122, Length=266, Percent_Identity=31.5789473684211, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI161078639, Length=266, Percent_Identity=31.5789473684211, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI281362242, Length=358, Percent_Identity=28.7709497206704, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648872, Length=358, Percent_Identity=28.7709497206704, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI20130355, Length=266, Percent_Identity=25.9398496240602, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 42765; Mature: 42765

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRARFTGILDRVQTTRHTRPERDTAMTKPSPASFRIAAIAGDGIGNEVLPEGLRVVEAA
CCCHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
ARKFDLLIEVRHFEWANCDYYLRHGKMMPDDWKQQLDGFDAIYFGAVGWPDKVPDHISLW
HHHHHEEEEEECCCCCCCHHHEECCCCCCHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
GSLLKFRREFDQYVNLRPVRLLPGVPCPLAGKKPGDIDFYVVRENTEGEYSSVGGRMYEG
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCCHHHCCCEECCC
TERELVMQQSIFSRHGTDRILKYAFELAQSRPRKKLTSATKSNGIAVSMPWWDERTAAMG
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCHHHHHC
AQYPEIEWDSQHIDILCARFVLQPERFDVVVASNLFGDLLSDLGPACTGTIGLAGSANLN
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCHHCCCEECCCCCCCC
PERKFPSLFEPVHGSAPDIFGKQIANPIGMIWSGAMMLDFLGGEAGRQAHDAILAAIETV
CCCCCCHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LREGPLTPDAGGKAGTSDVGKAIAEAV
HHHCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MIRARFTGILDRVQTTRHTRPERDTAMTKPSPASFRIAAIAGDGIGNEVLPEGLRVVEAA
CCCHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
ARKFDLLIEVRHFEWANCDYYLRHGKMMPDDWKQQLDGFDAIYFGAVGWPDKVPDHISLW
HHHHHEEEEEECCCCCCCHHHEECCCCCCHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
GSLLKFRREFDQYVNLRPVRLLPGVPCPLAGKKPGDIDFYVVRENTEGEYSSVGGRMYEG
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCCHHHCCCEECCC
TERELVMQQSIFSRHGTDRILKYAFELAQSRPRKKLTSATKSNGIAVSMPWWDERTAAMG
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCHHHHHC
AQYPEIEWDSQHIDILCARFVLQPERFDVVVASNLFGDLLSDLGPACTGTIGLAGSANLN
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCHHCCCEECCCCCCCC
PERKFPSLFEPVHGSAPDIFGKQIANPIGMIWSGAMMLDFLGGEAGRQAHDAILAAIETV
CCCCCCHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LREGPLTPDAGGKAGTSDVGKAIAEAV
HHHCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]