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The map label for this gene is hipO [H]

Identifier: 73542972

GI number: 73542972

Start: 3596940

End: 3598154

Strand: Direct

Name: hipO [H]

Synonym: Reut_A3289

Alternate gene names: 73542972

Gene position: 3596940-3598154 (Clockwise)

Preceding gene: 73542971

Following gene: 73542974

Centisome position: 94.49

GC content: 65.35

Gene sequence:

>1215_bases
ATGACTCGCGCACCCACGCTCAAGCCGTTCCAGTACCTGCCGCACCTGCTGCCGAATATCAGCATCGACGCCGAGACCTT
CGTCGGCATCCGCCGCCAGATCCACTCGCATCCCGAACTCGGTTTCGAGGTCGGCGCGACCAGCAAGCTCGTGGCCACTC
TGCTGAGCGAATGGGGCTACGAAGTGCATACCGGTATCGGCAAGAGCGGCGTGGTCGGACAGTTGAAGCGCGGCACCGGC
AAGCGCCGTCTCGGCATCCGTGCCGACATGGACGCGCTGCCGGTCATCGAAGCCACGGGGCTACCCTATGCGAGCCAGCT
GCACGGCAAGATGCATGCGTGCGGCCATGATGGTCATACGGCGATCCTGCTCGCTGCTGCGAAGGCAATCGCCGACCATC
CCGATTTCGACGGCACGCTGAACCTGATCTTCCAGCCCGACGAGGAGAACCTGTGCGGCGCGCGCGCAATGATCGAGGAT
GGATTGTTCGAACGCTTCCCGTGCGATGCGGTGTTCGCGCTGCACAACATGCCGGGTGTGCCGGCGGGCACGTTCCGCGT
GCTGCCCGGGCCGGTCAGCCTGTCGTCCGATGTGGCTGACGTGACCATCAAGGGCGTGGGTGGCCACGGTGCCATGCCGC
ACCGCGCGCGCGATCCGATCGCTGCGTCGGCGGCGATCGTCACTGCGCTTCAGACCGTGGTCGCGCGCAATGTCGCGCCT
GATGACACGGCGGTGGTGTCGGTCGGCTTCATTCGTGGAGGCGCTACGCATAACGTGATTCCCGAGTCCGTGACGATCGG
ACTGAACGTGCGCGCGGGACGTCCGGAAACGCGTGCAATGGTCGAGCAGCGCATCCGCGAAATCGTGTCGCTGACAGCGC
AGGCGCATGGTGTGGAAGCGCAGATCGATTACCGGCAGCTCACGCCGCCGATGGTCAATGCCGAAGCGGAGACGCAGCTC
GCGCAGCGCGTGTGTGCGGAACTGGTCGGTGCGGAGAATGTGGTCACGCAGGCGCCAAAGGGTCTGAACGGGAGCGAGGA
CTTCGCGTGGATGCTGAATCAGGTGCCGGGGTGCTATCTGATCCTGGGCAACGGTGAAGGGGAGTTCGGCGGGTGCATGG
TGCACAACCCGGGGTACGACTTCAACGATGCCGTGCTGCCGCTCGGTGCAGCGGCGTGGGTGAGGCTGACGCAGGCGTAC
CTGGCTGCGCAGTGA

Upstream 100 bases:

>100_bases
GGTACATGCTGGTCTGCGGCGCCATCACACTGATCGCCGTGCTCAGCATCCGCGAACGCCGCGCGCACTGATTTCCACCA
TTCATCCGGAGACCCGATCC

Downstream 100 bases:

>100_bases
ATCCGCCGCTTCTTACTGTGCGGGGGCCGGCTCGGTCGATGGTGCCAGGCCCGCGCGCTTCGCGCCCAGCCGCGCGTTGA
TCTCCGCCGCTTCCGCAAGC

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTRAPTLKPFQYLPHLLPNISIDAETFVGIRRQIHSHPELGFEVGATSKLVATLLSEWGYEVHTGIGKSGVVGQLKRGTG
KRRLGIRADMDALPVIEATGLPYASQLHGKMHACGHDGHTAILLAAAKAIADHPDFDGTLNLIFQPDEENLCGARAMIED
GLFERFPCDAVFALHNMPGVPAGTFRVLPGPVSLSSDVADVTIKGVGGHGAMPHRARDPIAASAAIVTALQTVVARNVAP
DDTAVVSVGFIRGGATHNVIPESVTIGLNVRAGRPETRAMVEQRIREIVSLTAQAHGVEAQIDYRQLTPPMVNAEAETQL
AQRVCAELVGAENVVTQAPKGLNGSEDFAWMLNQVPGCYLILGNGEGEFGGCMVHNPGYDFNDAVLPLGAAAWVRLTQAY
LAAQ

Sequences:

>Translated_404_residues
MTRAPTLKPFQYLPHLLPNISIDAETFVGIRRQIHSHPELGFEVGATSKLVATLLSEWGYEVHTGIGKSGVVGQLKRGTG
KRRLGIRADMDALPVIEATGLPYASQLHGKMHACGHDGHTAILLAAAKAIADHPDFDGTLNLIFQPDEENLCGARAMIED
GLFERFPCDAVFALHNMPGVPAGTFRVLPGPVSLSSDVADVTIKGVGGHGAMPHRARDPIAASAAIVTALQTVVARNVAP
DDTAVVSVGFIRGGATHNVIPESVTIGLNVRAGRPETRAMVEQRIREIVSLTAQAHGVEAQIDYRQLTPPMVNAEAETQL
AQRVCAELVGAENVVTQAPKGLNGSEDFAWMLNQVPGCYLILGNGEGEFGGCMVHNPGYDFNDAVLPLGAAAWVRLTQAY
LAAQ
>Mature_403_residues
TRAPTLKPFQYLPHLLPNISIDAETFVGIRRQIHSHPELGFEVGATSKLVATLLSEWGYEVHTGIGKSGVVGQLKRGTGK
RRLGIRADMDALPVIEATGLPYASQLHGKMHACGHDGHTAILLAAAKAIADHPDFDGTLNLIFQPDEENLCGARAMIEDG
LFERFPCDAVFALHNMPGVPAGTFRVLPGPVSLSSDVADVTIKGVGGHGAMPHRARDPIAASAAIVTALQTVVARNVAPD
DTAVVSVGFIRGGATHNVIPESVTIGLNVRAGRPETRAMVEQRIREIVSLTAQAHGVEAQIDYRQLTPPMVNAEAETQLA
QRVCAELVGAENVVTQAPKGLNGSEDFAWMLNQVPGCYLILGNGEGEFGGCMVHNPGYDFNDAVLPLGAAAWVRLTQAYL
AAQ

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=442, Percent_Identity=26.4705882352941, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42898; Mature: 42766

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAPTLKPFQYLPHLLPNISIDAETFVGIRRQIHSHPELGFEVGATSKLVATLLSEWGY
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHCCC
EVHTGIGKSGVVGQLKRGTGKRRLGIRADMDALPVIEATGLPYASQLHGKMHACGHDGHT
EEECCCCCCCCHHHHHCCCCCCEECEECCCCCCCEEEECCCCCHHHHCCHHHCCCCCCCH
AILLAAAKAIADHPDFDGTLNLIFQPDEENLCGARAMIEDGLFERFPCDAVFALHNMPGV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHCCCHHHHHHHCCCCC
PAGTFRVLPGPVSLSSDVADVTIKGVGGHGAMPHRARDPIAASAAIVTALQTVVARNVAP
CCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DDTAVVSVGFIRGGATHNVIPESVTIGLNVRAGRPETRAMVEQRIREIVSLTAQAHGVEA
CCCEEEEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEE
QIDYRQLTPPMVNAEAETQLAQRVCAELVGAENVVTQAPKGLNGSEDFAWMLNQVPGCYL
EECHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCCCEEE
ILGNGEGEFGGCMVHNPGYDFNDAVLPLGAAAWVRLTQAYLAAQ
EEECCCCCCCCEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRAPTLKPFQYLPHLLPNISIDAETFVGIRRQIHSHPELGFEVGATSKLVATLLSEWGY
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHCCC
EVHTGIGKSGVVGQLKRGTGKRRLGIRADMDALPVIEATGLPYASQLHGKMHACGHDGHT
EEECCCCCCCCHHHHHCCCCCCEECEECCCCCCCEEEECCCCCHHHHCCHHHCCCCCCCH
AILLAAAKAIADHPDFDGTLNLIFQPDEENLCGARAMIEDGLFERFPCDAVFALHNMPGV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHCCCHHHHHHHCCCCC
PAGTFRVLPGPVSLSSDVADVTIKGVGGHGAMPHRARDPIAASAAIVTALQTVVARNVAP
CCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DDTAVVSVGFIRGGATHNVIPESVTIGLNVRAGRPETRAMVEQRIREIVSLTAQAHGVEA
CCCEEEEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEE
QIDYRQLTPPMVNAEAETQLAQRVCAELVGAENVVTQAPKGLNGSEDFAWMLNQVPGCYL
EECHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCCCEEE
ILGNGEGEFGGCMVHNPGYDFNDAVLPLGAAAWVRLTQAYLAAQ
EEECCCCCCCCEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]