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The map label for this gene is gcl [H]

Identifier: 73542974

GI number: 73542974

Start: 3599196

End: 3600980

Strand: Direct

Name: gcl [H]

Synonym: Reut_A3291

Alternate gene names: 73542974

Gene position: 3599196-3600980 (Clockwise)

Preceding gene: 73542972

Following gene: 73542975

Centisome position: 94.55

GC content: 65.1

Gene sequence:

>1785_bases
ATGGCAAAAATGAGAGCAATCGACGCGGCCATCGCGGTGCTGGAGAAGGAAGGCGTGACCACGGCGTTCGGCGTGCCGGG
CGCGGCGATCAACCCGTTCTACTCGGCGATGCGCAAGTCGGGCAACATCAGCCACGTGCTGGCCCGCCACGTCGAAGGCG
CCTCGCACATGGCCGAAGGCTACACCCGTGCCGAGCCGGGCAATATCGGCGTCTGCGTCGGTACCTCGGGCCCGGCTGGC
ACCGACATGATCACCGGCCTGTACTCGGCCTGGGCGGATTCGATCCCCATTCTCTGCATTACCGGCCAGGCGCCGCGCGC
CCGCCTGTACAAGGAAGATTTCCAGGCCGTCGATATCGAATCGATCGCCAAGCCCGTCACCAAGTGGGCCGTGACCGTGC
GTGAGCCGGCCCTGGTGCCGCAAGTGTTCCAGCAAGCGTTCCACATCATGCGTTCGGGCCGTCCGGGTCCGGTGCTGATC
GACCTGCCGTTCGACGTGCAGGTGGCCGAGATCGAATTCGATGTCGAGACCTACCAGTCGCTGCAGCCGTACAAGCCGGC
CGCCTCGCGCGCCCAGATCGAGAAGGCCATCGCCATGCTCAACGCGGCCGAGCGCCCGCTGATCGTCTGCGGCGGCGGCG
TGATCAACGCTGACGCGTCCGATCTGCTGGTCGAGTTCGCCGAGCTGGTCAACGTGCCGGTGGTGCCGACCCTGATGGGC
TGGGGCGTGCTCGCCGACGACCACCCGCTGCAAGCCGGCATGGTCGGCCTGCAGACCTCGCACCGCTATGGCAACGCCAC
GCTGCTCGCCTCGGACTTCGTGATGGGCATCGGCAACCGCTGGGCCAACCGCCACACCGGCAGCGTCGACGTCTACACCA
AGGGCCGCAAGTTTGTGCACGTCGATATCGAACCCACCCAGATCGGCCGCGTGTTCGGCCCGGATCTGGGCATCGTGTCG
GACGCCAAGGCTGCGCTGGAACTGTTCGTGGAAGTCGCCCGCGAAATGAAGATGGCCGGCCGCCTGCCGTGCCGCAAGTC
GTGGGTTGCCGATGTGCAGAAGCGCCGCCGCACCATGCAGCGCAAGAGCGATTTCGACAACGTGCCGGTCAAGCCGCAGC
GCGTGTACCGCGAGATGAACCAGTACTTCCCTCGCGACGTGCGCTACGTCAGCACGATCGGCCTGTCGCAGATCGCCGCG
GCGCAGTTCCTGTCGGTCAACGAGCCGCGCCACTGGATCAACTGCGGCCAGGCTGGCCCGCTGGGCTGGACCATTCCCGC
CGCCATCGGCGTGAAGACCGCATCGCCGAACTCGGACGTGGTGGCGATCTCGGGCGACTACGACTTCCAGTTCATGATCG
AAGAACTGGCCGTGGCCGCGCAGTTCAAGGTGCCGTACATCCACGTGGTGGTGAACAACTCCTACCTCGGCCTGATCCGT
CAGGCACAGCGCGGCTTCGAGATGGATTACTGCGTGCAGCTCGCGTTCGACAACATCAACGCGCCGGAACTGAACGGCTA
CGGCGTGGACCACGTCAAGGTCGTCGAAGGCCTGGGCTGCAAGGCGATCCGCGTGTTCAAGCCGGAAGACATCGCGCCGG
CCTTCGCCGAAGCGCGTGACCTGATGGCCGAGTTCTCGGTGCCGGTGGTGGTCGAAGTGATCCTGGAGCGCGTGACCAAC
ATCGCAATGGGCACCGAAATCGACAACATCAACGAGTTCGAGCCGATCGAAGACATCGAGGACGCCGACGAGGCCATCCT
GAACGAAGAAGTGGAAACGGCGTAA

Upstream 100 bases:

>100_bases
CAGGTATCGAATCAACTGATCTCGCTTCCCTTTATCCGACACCCGTTGCGGGAATAGGATCAACTCCAACGGAAAACCCA
CCCCACCTGGAGACAAGATC

Downstream 100 bases:

>100_bases
GCAAGCCTCCAAACGCGGCGCGGGCGACAGGCCCGCGCTGCTGTCAGCGCCCCCTGTTTTTGCAGCAGAAACGAAAGCAG
TAGCGGCTCCGCACCGGCGG

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MAKMRAIDAAIAVLEKEGVTTAFGVPGAAINPFYSAMRKSGNISHVLARHVEGASHMAEGYTRAEPGNIGVCVGTSGPAG
TDMITGLYSAWADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPQVFQQAFHIMRSGRPGPVLI
DLPFDVQVAEIEFDVETYQSLQPYKPAASRAQIEKAIAMLNAAERPLIVCGGGVINADASDLLVEFAELVNVPVVPTLMG
WGVLADDHPLQAGMVGLQTSHRYGNATLLASDFVMGIGNRWANRHTGSVDVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVEVAREMKMAGRLPCRKSWVADVQKRRRTMQRKSDFDNVPVKPQRVYREMNQYFPRDVRYVSTIGLSQIAA
AQFLSVNEPRHWINCGQAGPLGWTIPAAIGVKTASPNSDVVAISGDYDFQFMIEELAVAAQFKVPYIHVVVNNSYLGLIR
QAQRGFEMDYCVQLAFDNINAPELNGYGVDHVKVVEGLGCKAIRVFKPEDIAPAFAEARDLMAEFSVPVVVEVILERVTN
IAMGTEIDNINEFEPIEDIEDADEAILNEEVETA

Sequences:

>Translated_594_residues
MAKMRAIDAAIAVLEKEGVTTAFGVPGAAINPFYSAMRKSGNISHVLARHVEGASHMAEGYTRAEPGNIGVCVGTSGPAG
TDMITGLYSAWADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPQVFQQAFHIMRSGRPGPVLI
DLPFDVQVAEIEFDVETYQSLQPYKPAASRAQIEKAIAMLNAAERPLIVCGGGVINADASDLLVEFAELVNVPVVPTLMG
WGVLADDHPLQAGMVGLQTSHRYGNATLLASDFVMGIGNRWANRHTGSVDVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVEVAREMKMAGRLPCRKSWVADVQKRRRTMQRKSDFDNVPVKPQRVYREMNQYFPRDVRYVSTIGLSQIAA
AQFLSVNEPRHWINCGQAGPLGWTIPAAIGVKTASPNSDVVAISGDYDFQFMIEELAVAAQFKVPYIHVVVNNSYLGLIR
QAQRGFEMDYCVQLAFDNINAPELNGYGVDHVKVVEGLGCKAIRVFKPEDIAPAFAEARDLMAEFSVPVVVEVILERVTN
IAMGTEIDNINEFEPIEDIEDADEAILNEEVETA
>Mature_593_residues
AKMRAIDAAIAVLEKEGVTTAFGVPGAAINPFYSAMRKSGNISHVLARHVEGASHMAEGYTRAEPGNIGVCVGTSGPAGT
DMITGLYSAWADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPQVFQQAFHIMRSGRPGPVLID
LPFDVQVAEIEFDVETYQSLQPYKPAASRAQIEKAIAMLNAAERPLIVCGGGVINADASDLLVEFAELVNVPVVPTLMGW
GVLADDHPLQAGMVGLQTSHRYGNATLLASDFVMGIGNRWANRHTGSVDVYTKGRKFVHVDIEPTQIGRVFGPDLGIVSD
AKAALELFVEVAREMKMAGRLPCRKSWVADVQKRRRTMQRKSDFDNVPVKPQRVYREMNQYFPRDVRYVSTIGLSQIAAA
QFLSVNEPRHWINCGQAGPLGWTIPAAIGVKTASPNSDVVAISGDYDFQFMIEELAVAAQFKVPYIHVVVNNSYLGLIRQ
AQRGFEMDYCVQLAFDNINAPELNGYGVDHVKVVEGLGCKAIRVFKPEDIAPAFAEARDLMAEFSVPVVVEVILERVTNI
AMGTEIDNINEFEPIEDIEDADEAILNEEVETA

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.5583038869258, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI21361361, Length=600, Percent_Identity=23.8333333333333, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1786717, Length=578, Percent_Identity=79.4117647058823, Blast_Score=961, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=542, Percent_Identity=33.0258302583026, Blast_Score=315, Evalue=8e-87,
Organism=Escherichia coli, GI1790104, Length=477, Percent_Identity=35.0104821802935, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=25.5009107468124, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1788716, Length=480, Percent_Identity=27.2916666666667, Blast_Score=139, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=556, Percent_Identity=25.8992805755396, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17531299, Length=518, Percent_Identity=23.7451737451737, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17531301, Length=518, Percent_Identity=23.7451737451737, Blast_Score=108, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=593, Percent_Identity=31.0286677908938, Blast_Score=269, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6320816, Length=565, Percent_Identity=23.716814159292, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323163, Length=493, Percent_Identity=21.2981744421907, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323073, Length=534, Percent_Identity=20.2247191011236, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19922626, Length=490, Percent_Identity=26.1224489795918, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64929; Mature: 64798

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAIDAAIAVLEKEGVTTAFGVPGAAINPFYSAMRKSGNISHVLARHVEGASHMAEG
CCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCC
YTRAEPGNIGVCVGTSGPAGTDMITGLYSAWADSIPILCITGQAPRARLYKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPQVFQQAFHIMRSGRPGPVLIDLPFDVQVAEIEFDVETYQS
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECHHHHHC
LQPYKPAASRAQIEKAIAMLNAAERPLIVCGGGVINADASDLLVEFAELVNVPVVPTLMG
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCEECCCHHHHHHHHHHHHCCCCHHHHHC
WGVLADDHPLQAGMVGLQTSHRYGNATLLASDFVMGIGNRWANRHTGSVDVYTKGRKFVH
CCEECCCCCCHHCEEEEECCCCCCCEEEEHHHHHHHHCCHHCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVEVAREMKMAGRLPCRKSWVADVQKRRRTMQ
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKSDFDNVPVKPQRVYREMNQYFPRDVRYVSTIGLSQIAAAQFLSVNEPRHWINCGQAGP
HHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHCCCCCHHHCCCCCCCC
LGWTIPAAIGVKTASPNSDVVAISGDYDFQFMIEELAVAAQFKVPYIHVVVNNSYLGLIR
CCEECHHHHEEEECCCCCCEEEEECCCCHHHHHHHHHHHHEECCCEEEEEECCCHHHHHH
QAQRGFEMDYCVQLAFDNINAPELNGYGVDHVKVVEGLGCKAIRVFKPEDIAPAFAEARD
HHHHCCCHHHEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
LMAEFSVPVVVEVILERVTNIAMGTEIDNINEFEPIEDIEDADEAILNEEVETA
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKMRAIDAAIAVLEKEGVTTAFGVPGAAINPFYSAMRKSGNISHVLARHVEGASHMAEG
CCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCC
YTRAEPGNIGVCVGTSGPAGTDMITGLYSAWADSIPILCITGQAPRARLYKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPQVFQQAFHIMRSGRPGPVLIDLPFDVQVAEIEFDVETYQS
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECHHHHHC
LQPYKPAASRAQIEKAIAMLNAAERPLIVCGGGVINADASDLLVEFAELVNVPVVPTLMG
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCEECCCHHHHHHHHHHHHCCCCHHHHHC
WGVLADDHPLQAGMVGLQTSHRYGNATLLASDFVMGIGNRWANRHTGSVDVYTKGRKFVH
CCEECCCCCCHHCEEEEECCCCCCCEEEEHHHHHHHHCCHHCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVEVAREMKMAGRLPCRKSWVADVQKRRRTMQ
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKSDFDNVPVKPQRVYREMNQYFPRDVRYVSTIGLSQIAAAQFLSVNEPRHWINCGQAGP
HHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHCCCCCHHHCCCCCCCC
LGWTIPAAIGVKTASPNSDVVAISGDYDFQFMIEELAVAAQFKVPYIHVVVNNSYLGLIR
CCEECHHHHEEEECCCCCCEEEEECCCCHHHHHHHHHHHHEECCCEEEEEECCCHHHHHH
QAQRGFEMDYCVQLAFDNINAPELNGYGVDHVKVVEGLGCKAIRVFKPEDIAPAFAEARD
HHHHCCCHHHEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
LMAEFSVPVVVEVILERVTNIAMGTEIDNINEFEPIEDIEDADEAILNEEVETA
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]