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The map label for this gene is citH [H]

Identifier: 73542971

GI number: 73542971

Start: 3595615

End: 3596910

Strand: Direct

Name: citH [H]

Synonym: Reut_A3288

Alternate gene names: 73542971

Gene position: 3595615-3596910 (Clockwise)

Preceding gene: 73542967

Following gene: 73542972

Centisome position: 94.46

GC content: 67.36

Gene sequence:

>1296_bases
ATGAACGACGCCACCCTCGCCGCCGGCAGCGGCACGATTGCCAAGCCGGCGCGACCGCAAAGCCAGTTCCGCCTGATCGC
CGCCTGTTCGCTCGGCAATGCGCTCGAGATGTTCGACTTCACCGTCTACAGCTTCTTCTCGCTGCTGATTGGCAAGCTGT
TCTTCCCGTCCGACAACCCATACGGCTCGCTGCTGCTGGCTGTCGCAACCTTCGGCATCGGCTTCGTGATGCGCCCGCTC
GGTGGTGTGGTGATCGGCAGCTATGCCGACCGCCGTGGCCGCAAGGCCGCCATGACGCTGACCATCGGCCTGATGGTCGC
CGGCACGCTGTGCATCGCGCTCGCGCCGCCGTACGCATCGGCTGGCGTGTTCGGCTCGCTGATGATCCTGGCCGGGCGCC
TGCTGCAGGGCTTCTCGCTCGGCGGCGAGATCGGCGCATCGACGGCCATGCTGATGGAGTCCGGCGGCGTGCGCGGCCGC
GGCTGGCGCGTGAGCTGGCAGCTTGGCAGCCAGGGCATCTCGGCGATGCTGGGTGCGCTCACAGGTGCGAGCCTGTATGC
ACTGCTGCCGACCGAATCGCTCGAAAGCTGGGGCTGGCGCCTGCCGTTCCTGGTGGGCCTGCTGATCGCGCCCGTGGGCC
TCTACATCCGCGCCAATCTCGACGAAACGCACCAGGCCGACGAACATGCGCCGAGTCCGCTCGGCGAGCTGTTCCGCAAC
CACTGGGCGACGGTCGTGAAAGGCATCCTGGCCACCACGGCCGGCACCGCGACGATGTACCTGGTGGTCTTCTTCATGCC
GACCTACATGATCCGCGTGCTCAAGATGCCGCCGTCGCTGTCGTTCCTGTCCGGCTGCGTGACGGGGCTCACGCTGACGG
TGGTGTCGCTCGTCGCCGGCCGCCTCGCTGACCGCCTGACGAACCGCAAGCCGCTGGTGGTCGCATCGCTCGTCTTCAGC
ATGCTCGCCGTGTATCCCGCGTTCTGGCTGATCAACGCGCATCCGAGCGTGCCGCTGGTGCTGTGCCTGTCGGCGCTGCT
GACCGCCGGCATCAATCTCGGCACGACGCCAATGTTCCTGATGATGCTGGAAATGCTGCCCGCCGGCGTGCGCGCCAGCG
GCCTGTCGGTCATCTACAGCGTCGGCGTGACCGTGTTCGGCGGCTCGTCGCAATTTATCGTCACGTGGCTGCTCTCGCGC
ACCGGCAACCCGCTGTCGCCGGCGTGGTACATGCTGGTCTGCGGCGCCATCACACTGATCGCCGTGCTCAGCATCCGCGA
ACGCCGCGCGCACTGA

Upstream 100 bases:

>100_bases
CAGTCATCGCCACCCCATGGCCAGCGCGGATTCCCACCAACCGGCAAGGAACAAGACCGGCCCCGCGTGCGCCTCCTACC
CACCAGTTCGTGGAGACCGC

Downstream 100 bases:

>100_bases
TTTCCACCATTCATCCGGAGACCCGATCCATGACTCGCGCACCCACGCTCAAGCCGTTCCAGTACCTGCCGCACCTGCTG
CCGAATATCAGCATCGACGC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNDATLAAGSGTIAKPARPQSQFRLIAACSLGNALEMFDFTVYSFFSLLIGKLFFPSDNPYGSLLLAVATFGIGFVMRPL
GGVVIGSYADRRGRKAAMTLTIGLMVAGTLCIALAPPYASAGVFGSLMILAGRLLQGFSLGGEIGASTAMLMESGGVRGR
GWRVSWQLGSQGISAMLGALTGASLYALLPTESLESWGWRLPFLVGLLIAPVGLYIRANLDETHQADEHAPSPLGELFRN
HWATVVKGILATTAGTATMYLVVFFMPTYMIRVLKMPPSLSFLSGCVTGLTLTVVSLVAGRLADRLTNRKPLVVASLVFS
MLAVYPAFWLINAHPSVPLVLCLSALLTAGINLGTTPMFLMMLEMLPAGVRASGLSVIYSVGVTVFGGSSQFIVTWLLSR
TGNPLSPAWYMLVCGAITLIAVLSIRERRAH

Sequences:

>Translated_431_residues
MNDATLAAGSGTIAKPARPQSQFRLIAACSLGNALEMFDFTVYSFFSLLIGKLFFPSDNPYGSLLLAVATFGIGFVMRPL
GGVVIGSYADRRGRKAAMTLTIGLMVAGTLCIALAPPYASAGVFGSLMILAGRLLQGFSLGGEIGASTAMLMESGGVRGR
GWRVSWQLGSQGISAMLGALTGASLYALLPTESLESWGWRLPFLVGLLIAPVGLYIRANLDETHQADEHAPSPLGELFRN
HWATVVKGILATTAGTATMYLVVFFMPTYMIRVLKMPPSLSFLSGCVTGLTLTVVSLVAGRLADRLTNRKPLVVASLVFS
MLAVYPAFWLINAHPSVPLVLCLSALLTAGINLGTTPMFLMMLEMLPAGVRASGLSVIYSVGVTVFGGSSQFIVTWLLSR
TGNPLSPAWYMLVCGAITLIAVLSIRERRAH
>Mature_431_residues
MNDATLAAGSGTIAKPARPQSQFRLIAACSLGNALEMFDFTVYSFFSLLIGKLFFPSDNPYGSLLLAVATFGIGFVMRPL
GGVVIGSYADRRGRKAAMTLTIGLMVAGTLCIALAPPYASAGVFGSLMILAGRLLQGFSLGGEIGASTAMLMESGGVRGR
GWRVSWQLGSQGISAMLGALTGASLYALLPTESLESWGWRLPFLVGLLIAPVGLYIRANLDETHQADEHAPSPLGELFRN
HWATVVKGILATTAGTATMYLVVFFMPTYMIRVLKMPPSLSFLSGCVTGLTLTVVSLVAGRLADRLTNRKPLVVASLVFS
MLAVYPAFWLINAHPSVPLVLCLSALLTAGINLGTTPMFLMMLEMLPAGVRASGLSVIYSVGVTVFGGSSQFIVTWLLSR
TGNPLSPAWYMLVCGAITLIAVLSIRERRAH

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=424, Percent_Identity=30.6603773584906, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788942, Length=446, Percent_Identity=32.0627802690583, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788292, Length=411, Percent_Identity=29.683698296837, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=31.002331002331, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87082231, Length=426, Percent_Identity=26.5258215962441, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87082404, Length=378, Percent_Identity=25.9259259259259, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45694; Mature: 45694

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDATLAAGSGTIAKPARPQSQFRLIAACSLGNALEMFDFTVYSFFSLLIGKLFFPSDNP
CCCCEEECCCCCCCCCCCCHHHHEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
YGSLLLAVATFGIGFVMRPLGGVVIGSYADRRGRKAAMTLTIGLMVAGTLCIALAPPYAS
HHHHHHHHHHHHHHHHHHHHCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
AGVFGSLMILAGRLLQGFSLGGEIGASTAMLMESGGVRGRGWRVSWQLGSQGISAMLGAL
HHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHH
TGASLYALLPTESLESWGWRLPFLVGLLIAPVGLYIRANLDETHQADEHAPSPLGELFRN
HCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHCEEEECCCCHHHCCCCCCCCHHHHHHHH
HWATVVKGILATTAGTATMYLVVFFMPTYMIRVLKMPPSLSFLSGCVTGLTLTVVSLVAG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RLADRLTNRKPLVVASLVFSMLAVYPAFWLINAHPSVPLVLCLSALLTAGINLGTTPMFL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCHHHHH
MMLEMLPAGVRASGLSVIYSVGVTVFGGSSQFIVTWLLSRTGNPLSPAWYMLVCGAITLI
HHHHHCCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AVLSIRERRAH
HHHHHHHHCCC
>Mature Secondary Structure
MNDATLAAGSGTIAKPARPQSQFRLIAACSLGNALEMFDFTVYSFFSLLIGKLFFPSDNP
CCCCEEECCCCCCCCCCCCHHHHEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
YGSLLLAVATFGIGFVMRPLGGVVIGSYADRRGRKAAMTLTIGLMVAGTLCIALAPPYAS
HHHHHHHHHHHHHHHHHHHHCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
AGVFGSLMILAGRLLQGFSLGGEIGASTAMLMESGGVRGRGWRVSWQLGSQGISAMLGAL
HHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHH
TGASLYALLPTESLESWGWRLPFLVGLLIAPVGLYIRANLDETHQADEHAPSPLGELFRN
HCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHCEEEECCCCHHHCCCCCCCCHHHHHHHH
HWATVVKGILATTAGTATMYLVVFFMPTYMIRVLKMPPSLSFLSGCVTGLTLTVVSLVAG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RLADRLTNRKPLVVASLVFSMLAVYPAFWLINAHPSVPLVLCLSALLTAGINLGTTPMFL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCHHHHH
MMLEMLPAGVRASGLSVIYSVGVTVFGGSSQFIVTWLLSRTGNPLSPAWYMLVCGAITLI
HHHHHCCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AVLSIRERRAH
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]