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The map label for this gene is mscL

Identifier: 73542778

GI number: 73542778

Start: 3405732

End: 3406166

Strand: Direct

Name: mscL

Synonym: Reut_A3094

Alternate gene names: 73542778

Gene position: 3405732-3406166 (Clockwise)

Preceding gene: 73542772

Following gene: 73542780

Centisome position: 89.47

GC content: 55.4

Gene sequence:

>435_bases
ATGAGCATGATGTCGGAGTTCAAGACCTTTGCAATGCGCGGCAACGTGATCGACCTTGCGGTCGGCGTAATCATTGGCGC
GGCATTTGGAAAAATCGTCGATTCGGTGGTCAATGACCTGATCATGCCGGTCATCGGGCGTATCGTCGGCAAGCTGGATT
TCTCGAATATGTTTGTTACCTTGGCCGAACCGCCTCCCGGTACGCCCATGACGCTTGACGCGCTGAAAAAAGCTGGTGTG
CCCGTGTTTGCTTACGGGAATTTTCTGACTATCGTTGTCAACTTCGTGATTTTGGCCTTCATCATTTTCCTGATGGTGCG
GGCATTCAATAAGATGCGTGCGGAAGAGCCGGCCCCGGCAGAGCCACCGCCGCCGCCGGAGGATATCGTCCTGCTGCGTG
AAATCCGCGACAGCCTGAAAACCCGCCAGCCCTGA

Upstream 100 bases:

>100_bases
CAAGCCCGATAGCCGCATGCAAGCTTGCGTTCCGCGCATTGATGCGGCAGGCTTCTGAGCTTGAAACGTTCATTGCTTGC
AACAATAAGGAGAATGCCCC

Downstream 100 bases:

>100_bases
ACCGGCAAAACGTCGGAAGCAGGATTCTGACAAGACAACGGCCCCGATGGGGGCCGTTTTTTTTGACATGCGTCAAACAG
GATTGGGAATATCGATGAAG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MSMMSEFKTFAMRGNVIDLAVGVIIGAAFGKIVDSVVNDLIMPVIGRIVGKLDFSNMFVTLAEPPPGTPMTLDALKKAGV
PVFAYGNFLTIVVNFVILAFIIFLMVRAFNKMRAEEPAPAEPPPPPEDIVLLREIRDSLKTRQP

Sequences:

>Translated_144_residues
MSMMSEFKTFAMRGNVIDLAVGVIIGAAFGKIVDSVVNDLIMPVIGRIVGKLDFSNMFVTLAEPPPGTPMTLDALKKAGV
PVFAYGNFLTIVVNFVILAFIIFLMVRAFNKMRAEEPAPAEPPPPPEDIVLLREIRDSLKTRQP
>Mature_143_residues
SMMSEFKTFAMRGNVIDLAVGVIIGAAFGKIVDSVVNDLIMPVIGRIVGKLDFSNMFVTLAEPPPGTPMTLDALKKAGVP
VFAYGNFLTIVVNFVILAFIIFLMVRAFNKMRAEEPAPAEPPPPPEDIVLLREIRDSLKTRQP

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=143, Percent_Identity=46.8531468531469, Blast_Score=123, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_CUPPJ (Q46WM9)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_297298.1
- ProteinModelPortal:   Q46WM9
- SMR:   Q46WM9
- GeneID:   3609685
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A3094
- NMPDR:   fig|264198.3.peg.2250
- HOGENOM:   HBG727604
- OMA:   IQLLTEI
- ProtClustDB:   PRK13952
- BioCyc:   REUT264198:REUT_A3094-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15748; Mature: 15617

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x115aa9c8)-; HASH(0x12cfeb04)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMMSEFKTFAMRGNVIDLAVGVIIGAAFGKIVDSVVNDLIMPVIGRIVGKLDFSNMFVT
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
LAEPPPGTPMTLDALKKAGVPVFAYGNFLTIVVNFVILAFIIFLMVRAFNKMRAEEPAPA
ECCCCCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EPPPPPEDIVLLREIRDSLKTRQP
CCCCCHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure 
SMMSEFKTFAMRGNVIDLAVGVIIGAAFGKIVDSVVNDLIMPVIGRIVGKLDFSNMFVT
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
LAEPPPGTPMTLDALKKAGVPVFAYGNFLTIVVNFVILAFIIFLMVRAFNKMRAEEPAPA
ECCCCCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EPPPPPEDIVLLREIRDSLKTRQP
CCCCCHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA