BacMap

Definition	Ralstonia eutropha JMP134 chromosome 1, complete sequence.
Accession	NC_007347
Length	3,806,533

Click here to switch to the map view.

The map label for this gene is caiD [C]

Identifier: 73542772

GI number: 73542772

Start: 3400305

End: 3401093

Strand: Direct

Name: caiD [C]

Synonym: Reut_A3088

Alternate gene names: 73542772

Gene position: 3400305-3401093 (Clockwise)

Preceding gene: 73542771

Following gene: 73542778

Centisome position: 89.33

GC content: 64.89

Gene sequence:

>789_bases
ATGACCATGCGAGACAACATGAGCACCGTTACCTATGAAGTCCGCGACCGCGTCGCGGAAATCCTGCTGGACAGCCCGCC
CGTCAACGCCCTGAACGAAGGCATGATCGATGCGCTGCTGGACGCCCTGCACCGTGCGGCGAAGGACGATGCCGTACGCG
CGGTCATCCTCGGCAGCGCCGTGCCACGGCGCTTTTGCGCGGGCCTGCAACTCGACGCGCTGCACGGTGCATCGCCACAG
CGCGCGTACCAGTTGGTCGACAAGCTTTACAGCGGCTTGAACGAAGCGCAGTTCCGGCTGGGCAAGCCATCGATTGCGGC
AGTGGGCGGCACGGCGCGCGGCGGCGGCATGACGCTGGCGATTCACTGCGACATGATCGTCGCCGCCGATACCGCAACCT
TCGGCTATCCGGAGATCGACGTCGGCGTCACGCCCGCGATCCATTACACGCACCTGCCCCGCATCATCGGCCGCCATCGC
GCATTCGATCTGCTGTTCACGGGCCGCTCGTTCGGCGCCGATGAAGCCATGTCGCTCGGGCTGGTCAATCGCATCGTCCC
CGAAGCCGACGTCATGTGCGAAGCGCGTGCCCTCGCGCAGACGTTCTGCGACAAGTCCCCGGTCGTGATGCGGATGGGAC
GCACGGCATTCCTGCGCGCCAATGACAGCGACTACCGCCGCGGCATCGCTACGGCAGTGGAATCGTTCGGCAATGTCTTT
GCCACCGACGATGCCAGGGAAGGCATATCCGCATTTGTGGAGAAGCGCAAGCCGTACTGGCAGGCGTGA

Upstream 100 bases:

>100_bases
GCTATGACAACGATGCTATCGCCGCGTTGCATGCGAGCGGCGTGGTGGCAACCACGGGCCACACGGAATCCAACACGCCG
ACGCAACAGCGCGCCGCGGC

Downstream 100 bases:

>100_bases
ACACCGTCCGGGCGTCAGCGGTTATGGCCTCACGCCCTGCTTGTTCCGCGGCGCCGAATCGGCCGCAAAAACGACAAGGG
GTTACAAGCCAAATGGCGTG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTMRDNMSTVTYEVRDRVAEILLDSPPVNALNEGMIDALLDALHRAAKDDAVRAVILGSAVPRRFCAGLQLDALHGASPQ
RAYQLVDKLYSGLNEAQFRLGKPSIAAVGGTARGGGMTLAIHCDMIVAADTATFGYPEIDVGVTPAIHYTHLPRIIGRHR
AFDLLFTGRSFGADEAMSLGLVNRIVPEADVMCEARALAQTFCDKSPVVMRMGRTAFLRANDSDYRRGIATAVESFGNVF
ATDDAREGISAFVEKRKPYWQA

Sequences:

>Translated_262_residues
MTMRDNMSTVTYEVRDRVAEILLDSPPVNALNEGMIDALLDALHRAAKDDAVRAVILGSAVPRRFCAGLQLDALHGASPQ
RAYQLVDKLYSGLNEAQFRLGKPSIAAVGGTARGGGMTLAIHCDMIVAADTATFGYPEIDVGVTPAIHYTHLPRIIGRHR
AFDLLFTGRSFGADEAMSLGLVNRIVPEADVMCEARALAQTFCDKSPVVMRMGRTAFLRANDSDYRRGIATAVESFGNVF
ATDDAREGISAFVEKRKPYWQA
>Mature_261_residues
TMRDNMSTVTYEVRDRVAEILLDSPPVNALNEGMIDALLDALHRAAKDDAVRAVILGSAVPRRFCAGLQLDALHGASPQR
AYQLVDKLYSGLNEAQFRLGKPSIAAVGGTARGGGMTLAIHCDMIVAADTATFGYPEIDVGVTPAIHYTHLPRIIGRHRA
FDLLFTGRSFGADEAMSLGLVNRIVPEADVMCEARALAQTFCDKSPVVMRMGRTAFLRANDSDYRRGIATAVESFGNVFA
TDDAREGISAFVEKRKPYWQA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=262, Percent_Identity=34.3511450381679, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=29.9595141700405, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI4502327, Length=238, Percent_Identity=33.6134453781513, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI68989263, Length=193, Percent_Identity=34.1968911917098, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI150378541, Length=249, Percent_Identity=30.1204819277108, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI20127408, Length=190, Percent_Identity=30, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=28.4046692607004, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI25453481, Length=270, Percent_Identity=25.9259259259259, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI51538573, Length=270, Percent_Identity=25.9259259259259, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4757966, Length=234, Percent_Identity=28.2051282051282, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI51538581, Length=234, Percent_Identity=28.2051282051282, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI62530384, Length=246, Percent_Identity=26.0162601626016, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI4757968, Length=216, Percent_Identity=29.6296296296296, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI49355787, Length=216, Percent_Identity=29.6296296296296, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221307494, Length=215, Percent_Identity=29.3023255813954, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221136756, Length=215, Percent_Identity=29.3023255813954, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221136753, Length=215, Percent_Identity=29.3023255813954, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI116235487, Length=172, Percent_Identity=33.1395348837209, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI295842266, Length=243, Percent_Identity=25.5144032921811, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI45643119, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI260275230, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI260274832, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=33.969465648855, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=33.7164750957854, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=29.7709923664122, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082183, Length=247, Percent_Identity=27.1255060728745, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=199, Percent_Identity=29.1457286432161, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=29.7709923664122, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=26.8421052631579, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=34.4, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=31.7269076305221, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560910, Length=236, Percent_Identity=32.2033898305085, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=212, Percent_Identity=27.8301886792453, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=249, Percent_Identity=30.9236947791165, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=213, Percent_Identity=27.2300469483568, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=263, Percent_Identity=26.2357414448669, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=207, Percent_Identity=31.4009661835749, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=218, Percent_Identity=29.8165137614679, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=23.1660231660232, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=187, Percent_Identity=26.7379679144385, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=187, Percent_Identity=26.7379679144385, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25144276, Length=187, Percent_Identity=26.7379679144385, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=29.0155440414508, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=35.1778656126482, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI20129971, Length=260, Percent_Identity=32.6923076923077, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24653477, Length=260, Percent_Identity=32.6923076923077, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI19922422, Length=245, Percent_Identity=30.2040816326531, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21357171, Length=242, Percent_Identity=27.6859504132231, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19920382, Length=251, Percent_Identity=26.2948207171315, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24583165, Length=255, Percent_Identity=23.921568627451, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24650670, Length=230, Percent_Identity=24.7826086956522, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28464; Mature: 28333

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMRDNMSTVTYEVRDRVAEILLDSPPVNALNEGMIDALLDALHRAAKDDAVRAVILGSA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VPRRFCAGLQLDALHGASPQRAYQLVDKLYSGLNEAQFRLGKPSIAAVGGTARGGGMTLA
HHHHHHCCCCEEHHCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCEEEECCCCCCCCEEEE
IHCDMIVAADTATFGYPEIDVGVTPAIHYTHLPRIIGRHRAFDLLFTGRSFGADEAMSLG
EEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHH
LVNRIVPEADVMCEARALAQTFCDKSPVVMRMGRTAFLRANDSDYRRGIATAVESFGNVF
HHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHCCEE
ATDDAREGISAFVEKRKPYWQA
ECCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TMRDNMSTVTYEVRDRVAEILLDSPPVNALNEGMIDALLDALHRAAKDDAVRAVILGSA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VPRRFCAGLQLDALHGASPQRAYQLVDKLYSGLNEAQFRLGKPSIAAVGGTARGGGMTLA
HHHHHHCCCCEEHHCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCEEEECCCCCCCCEEEE
IHCDMIVAADTATFGYPEIDVGVTPAIHYTHLPRIIGRHRAFDLLFTGRSFGADEAMSLG
EEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHH
LVNRIVPEADVMCEARALAQTFCDKSPVVMRMGRTAFLRANDSDYRRGIATAVESFGNVF
HHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHCCEE
ATDDAREGISAFVEKRKPYWQA
ECCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA