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The map label for this gene is phcA [H]

Identifier: 73542523

GI number: 73542523

Start: 3110581

End: 3111567

Strand: Reverse

Name: phcA [H]

Synonym: Reut_A2838

Alternate gene names: 73542523

Gene position: 3111567-3110581 (Counterclockwise)

Preceding gene: 73542525

Following gene: 73542521

Centisome position: 81.74

GC content: 63.02

Gene sequence:

>987_bases
ATGGTGAACGTGGACACCAAGCTTCTTGTGATTTTCACCGAACTGCTGAGCAAGCGGAACGCGACCTATGTCGCGGAGAA
AATGCACATGACGGCCCCGGCCGTGTCGCATTCGCTTGGCCGGCTGCGCGAGATCTTCGACGATCCGCTTTTCATCCGTG
TGCCGCACGGGCTGACCCCGACGCCCCGCGCGCTCGAACTTGGCCCCAAGATCCGCGACATGCTGGACCTGTGGTCGTCG
ATCAATGAAGGCGATGCCGACAATTTCGATCCGGCGATCGCCACGGGCACGCTGAGCATCGGCTTTGCCGCGGAACTGGG
CGACACGGTGTTCAACCGTTTCGTGCTGCGCATCAAGCAGCTGGCACCGGAACTGCATATCAAGCTGGTCGAATCCCATT
CGTGGGAAACCGATGTGGCTTCGATGCGCGCGAACGAACTCGACCTGGCTTTTTCGCCGTTCCCGACGCGGCATCCGGAA
ATCGTCGAGGAGATGGTGACGTCGCTGAGCCTGTGGGTGTGCGCACGCAAGAACCATCCGCTGCTGAAAGGCCAGTGCAC
GCTGGAGCAATACCTCGACTGTGGCCATATCTTCATGGCGCATTCGGGCGGCGCGGGCCGGCCGGCTCCGTCGCTGATCC
CGCTCGACTACGCATTGCAGCAGCGAGGGCTCAAGCGCAACGCGACGCTGACCGTCCATTCGTGGCGTGCGCAGGCCGAG
CTGGCCGCGCAGACCGACATGATCTTCACGGTCAACGCACTGACCAAGGACATGGCCTGCGAGACCTACGGCCTCAAGGC
GTCTCCGCTGCCGGCCGAGCTCAACACCACAGTCGGTCTGAACATGTTCTGGCACCGCAGCCGCAATACGCACCCGATGC
TGGTGTGGGCGCGCGGCCTGTTCCGACAGGTAGTGGGCGAATTCGTCGGCATGCCGGCCGCACGGCAGCTGCGTATGGTT
GCCGAGCAGGACGTCGAAACCCCCTGA

Upstream 100 bases:

>100_bases
TTTCCACTTACACTAATAACGAGCCGTTGATATCTGATGTGCAGCCAGTGCAAGTCGCGGGGCCATCTAAGATCGGAATA
AATCGGAACAGGGCCTGATC

Downstream 100 bases:

>100_bases
TCGCAGCCTCAGGCTCATTCGGACCCAAGAAAAAACCGGCGCACAGGCGCCGGTTTTTTCTTGCTGCCATCCCGCCGCGC
ACGAGCGGTGGCCGGCTTAT

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: Virulence genes transcriptional regulator phcA [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MVNVDTKLLVIFTELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTPTPRALELGPKIRDMLDLWSS
INEGDADNFDPAIATGTLSIGFAAELGDTVFNRFVLRIKQLAPELHIKLVESHSWETDVASMRANELDLAFSPFPTRHPE
IVEEMVTSLSLWVCARKNHPLLKGQCTLEQYLDCGHIFMAHSGGAGRPAPSLIPLDYALQQRGLKRNATLTVHSWRAQAE
LAAQTDMIFTVNALTKDMACETYGLKASPLPAELNTTVGLNMFWHRSRNTHPMLVWARGLFRQVVGEFVGMPAARQLRMV
AEQDVETP

Sequences:

>Translated_328_residues
MVNVDTKLLVIFTELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTPTPRALELGPKIRDMLDLWSS
INEGDADNFDPAIATGTLSIGFAAELGDTVFNRFVLRIKQLAPELHIKLVESHSWETDVASMRANELDLAFSPFPTRHPE
IVEEMVTSLSLWVCARKNHPLLKGQCTLEQYLDCGHIFMAHSGGAGRPAPSLIPLDYALQQRGLKRNATLTVHSWRAQAE
LAAQTDMIFTVNALTKDMACETYGLKASPLPAELNTTVGLNMFWHRSRNTHPMLVWARGLFRQVVGEFVGMPAARQLRMV
AEQDVETP
>Mature_328_residues
MVNVDTKLLVIFTELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTPTPRALELGPKIRDMLDLWSS
INEGDADNFDPAIATGTLSIGFAAELGDTVFNRFVLRIKQLAPELHIKLVESHSWETDVASMRANELDLAFSPFPTRHPE
IVEEMVTSLSLWVCARKNHPLLKGQCTLEQYLDCGHIFMAHSGGAGRPAPSLIPLDYALQQRGLKRNATLTVHSWRAQAE
LAAQTDMIFTVNALTKDMACETYGLKASPLPAELNTTVGLNMFWHRSRNTHPMLVWARGLFRQVVGEFVGMPAARQLRMV
AEQDVETP

Specific function: Regulates the transcription of one or more of the genes involved in virulence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=286, Percent_Identity=24.1258741258741, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790147, Length=316, Percent_Identity=26.8987341772152, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081766, Length=307, Percent_Identity=22.4755700325733, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36605; Mature: 36605

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNVDTKLLVIFTELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTP
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
TPRALELGPKIRDMLDLWSSINEGDADNFDPAIATGTLSIGFAAELGDTVFNRFVLRIKQ
CCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEECEEEECHHHHHHHHHHHHHHHHHHH
LAPELHIKLVESHSWETDVASMRANELDLAFSPFPTRHPEIVEEMVTSLSLWVCARKNHP
HCHHHEEEEECCCCCCHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHEEECCCCC
LLKGQCTLEQYLDCGHIFMAHSGGAGRPAPSLIPLDYALQQRGLKRNATLTVHSWRAQAE
CEECCCCHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCHHHHH
LAAQTDMIFTVNALTKDMACETYGLKASPLPAELNTTVGLNMFWHRSRNTHPMLVWARGL
HHHHCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEHHHHHH
FRQVVGEFVGMPAARQLRMVAEQDVETP
HHHHHHHHHCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVNVDTKLLVIFTELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTP
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
TPRALELGPKIRDMLDLWSSINEGDADNFDPAIATGTLSIGFAAELGDTVFNRFVLRIKQ
CCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEECEEEECHHHHHHHHHHHHHHHHHHH
LAPELHIKLVESHSWETDVASMRANELDLAFSPFPTRHPEIVEEMVTSLSLWVCARKNHP
HCHHHEEEEECCCCCCHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHEEECCCCC
LLKGQCTLEQYLDCGHIFMAHSGGAGRPAPSLIPLDYALQQRGLKRNATLTVHSWRAQAE
CEECCCCHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCHHHHH
LAAQTDMIFTVNALTKDMACETYGLKASPLPAELNTTVGLNMFWHRSRNTHPMLVWARGL
HHHHCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEHHHHHH
FRQVVGEFVGMPAARQLRMVAEQDVETP
HHHHHHHHHCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8366033; 10913109; 11823852 [H]