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The map label for this gene is gapA [H]

Identifier: 73542525

GI number: 73542525

Start: 3112518

End: 3113516

Strand: Reverse

Name: gapA [H]

Synonym: Reut_A2840

Alternate gene names: 73542525

Gene position: 3113516-3112518 (Counterclockwise)

Preceding gene: 73542526

Following gene: 73542523

Centisome position: 81.79

GC content: 62.36

Gene sequence:

>999_bases
ATGACCATCAAGATCGGCATCAACGGCTTCGGCCGCATCGGGCGCATGGTATTCCGTGCAGCCGCCGCCAACTTCAAGGA
CATCGAAGTCGTTGCCATCAACGACCTGCTCGAGCCCGACTACCTCGCGTACATGCTGAAGTACGACTCGGTGCACGGCC
GTTTCGACGGTGAAGTGTCGGTCGACGGCAACACGCTGGTCGTCAACGGCAAGAAGATCCGCCTGACCGCCGTCAAGGAT
CCGGCCGAGCTGAAGTGGGGCGAAGTCGGCGCCGACGTGGTGATCGAGTCGACCGGCATCTTCCTGACCAAGGAAGGCGC
GCAGAAGCACATCGACGCGGGCGCCAAGAAGGTGATCATGTCGGCACCGTCGAAGGACGACACCCCGATGTTCGTGTACG
GCGTGAACCACGAAACGTACAAGGGCGAAGCGATCATCTCGAACGCTTCGTGCACCACGAACTGCCTGGCACCGGTTGCC
AAGGTGCTGAACGACAAGTGGGGCATCAAGCGCGGCCTGATGACCACCGTGCACGCTGCCACCGCCACGCAGAAGACCGT
CGACGGCCCGTCCAACAAGGACTGGCGCGGCGGCCGCGGCATCCTGGAAAACATCATCCCGTCGTCGACGGGCGCCGCCA
AGGCCGTGGGCGTGGTGATTCCGCAGCTGAACAAGAAGCTGACCGGCATGTCGTTCCGCGTGCCGACTTCGGACGTGTCC
GTGGTCGACCTGACCGTCGAACTGGAAAAGTCGGCGTCGTACGAAGAAATCTGCGCCGAGATGAAGGCCCAGAGCCAGGG
CGCGCTGAAGGGCGTGCTGGGCTACACCGAAGACAAGGTTGTTGCCACGGACTTCCGCGGCGATGCACGCACCTCGATCT
TCGACGCTGAAGCCGGCATCGCGCTGGACGGCACCTTTATCAAGGTCGTGAGCTGGTACGACAACGAGTGGGGCTACTCG
AACAAGTGCCTGGAAATGGCACGCGTGGTGGCCAAGTAA

Upstream 100 bases:

>100_bases
GAGAACGTCGTGCGGACCGTCAAGGACACGCTGCAATAAGAGTCGCGGCGGACAACCCGGCGCCCCAGTTTTCTAGCATC
AGGCATTTCAGGAGATAGAC

Downstream 100 bases:

>100_bases
TCCGGCTTGCCTGTCGTGAAAAAACGCGCCTTCGGGCGCGTTTTTCTTTGGGCGGGCGTCAAGCTACGCATCAGCCGGCG
GGCCGTGCCGCGAGCGGCCG

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTIKIGINGFGRIGRMVFRAAAANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVSVDGNTLVVNGKKIRLTAVKD
PAELKWGEVGADVVIESTGIFLTKEGAQKHIDAGAKKVIMSAPSKDDTPMFVYGVNHETYKGEAIISNASCTTNCLAPVA
KVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
VVDLTVELEKSASYEEICAEMKAQSQGALKGVLGYTEDKVVATDFRGDARTSIFDAEAGIALDGTFIKVVSWYDNEWGYS
NKCLEMARVVAK

Sequences:

>Translated_332_residues
MTIKIGINGFGRIGRMVFRAAAANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVSVDGNTLVVNGKKIRLTAVKD
PAELKWGEVGADVVIESTGIFLTKEGAQKHIDAGAKKVIMSAPSKDDTPMFVYGVNHETYKGEAIISNASCTTNCLAPVA
KVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
VVDLTVELEKSASYEEICAEMKAQSQGALKGVLGYTEDKVVATDFRGDARTSIFDAEAGIALDGTFIKVVSWYDNEWGYS
NKCLEMARVVAK
>Mature_331_residues
TIKIGINGFGRIGRMVFRAAAANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVSVDGNTLVVNGKKIRLTAVKDP
AELKWGEVGADVVIESTGIFLTKEGAQKHIDAGAKKVIMSAPSKDDTPMFVYGVNHETYKGEAIISNASCTTNCLAPVAK
VLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVSV
VDLTVELEKSASYEEICAEMKAQSQGALKGVLGYTEDKVVATDFRGDARTSIFDAEAGIALDGTFIKVVSWYDNEWGYSN
KCLEMARVVAK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=333, Percent_Identity=60.0600600600601, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI7657116, Length=329, Percent_Identity=56.2310030395137, Blast_Score=386, Evalue=1e-107,
Organism=Escherichia coli, GI1788079, Length=332, Percent_Identity=70.7831325301205, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1789295, Length=335, Percent_Identity=39.4029850746269, Blast_Score=254, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17534677, Length=336, Percent_Identity=61.3095238095238, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17534679, Length=336, Percent_Identity=61.3095238095238, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32566163, Length=335, Percent_Identity=60.8955223880597, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17568413, Length=335, Percent_Identity=60.8955223880597, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322409, Length=332, Percent_Identity=62.0481927710843, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321631, Length=332, Percent_Identity=61.7469879518072, Blast_Score=414, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322468, Length=332, Percent_Identity=61.4457831325301, Blast_Score=412, Evalue=1e-116,
Organism=Drosophila melanogaster, GI17933600, Length=327, Percent_Identity=59.9388379204893, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI18110149, Length=327, Percent_Identity=59.9388379204893, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI85725000, Length=327, Percent_Identity=59.6330275229358, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI22023983, Length=327, Percent_Identity=59.6330275229358, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI19922412, Length=324, Percent_Identity=61.1111111111111, Blast_Score=393, Evalue=1e-109,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35779; Mature: 35648

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKIGINGFGRIGRMVFRAAAANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVS
CEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHEEEEEECCCCCCCCCEEE
VDGNTLVVNGKKIRLTAVKDPAELKWGEVGADVVIESTGIFLTKEGAQKHIDAGAKKVIM
ECCCEEEEECCEEEEEEECCCCCCCCCCCCCEEEEECCCEEEECCCCHHHHCCCCCEEEE
SAPSKDDTPMFVYGVNHETYKGEAIISNASCTTNCLAPVAKVLNDKWGIKRGLMTTVHAA
ECCCCCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
HCCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHEEEEEECCCCCCCCCCEEECCCCCCE
VVDLTVELEKSASYEEICAEMKAQSQGALKGVLGYTEDKVVATDFRGDARTSIFDAEAGI
EEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEECCCCE
ALDGTFIKVVSWYDNEWGYSNKCLEMARVVAK
EECCEEEEEEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TIKIGINGFGRIGRMVFRAAAANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVS
EEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHEEEEEECCCCCCCCCEEE
VDGNTLVVNGKKIRLTAVKDPAELKWGEVGADVVIESTGIFLTKEGAQKHIDAGAKKVIM
ECCCEEEEECCEEEEEEECCCCCCCCCCCCCEEEEECCCEEEECCCCHHHHCCCCCEEEE
SAPSKDDTPMFVYGVNHETYKGEAIISNASCTTNCLAPVAKVLNDKWGIKRGLMTTVHAA
ECCCCCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVS
HCCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHEEEEEECCCCCCCCCCEEECCCCCCE
VVDLTVELEKSASYEEICAEMKAQSQGALKGVLGYTEDKVVATDFRGDARTSIFDAEAGI
EEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEECCCCE
ALDGTFIKVVSWYDNEWGYSNKCLEMARVVAK
EECCEEEEEEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852; 8366033 [H]