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The map label for this gene is hoxA [H]

Identifier: 73542497

GI number: 73542497

Start: 3083051

End: 3084058

Strand: Reverse

Name: hoxA [H]

Synonym: Reut_A2812

Alternate gene names: 73542497

Gene position: 3084058-3083051 (Counterclockwise)

Preceding gene: 73542498

Following gene: 73542491

Centisome position: 81.02

GC content: 66.47

Gene sequence:

>1008_bases
ATGAGCGAACCGAATGCCACCCAGGCACCACCCCCGGCGATTCTGTTCGTCGACGACGAAGCCACAGCCGTCAAATACTT
CCATCGCGCGATCGGCGCCCTGGCCCCGGTGGTGACGGCCGGCTCCGTGGAGGAGGGCAAGACGCTGCTCGAGGCGCACG
CCGACAGCCTCGCGGTGCTGGTGTCGGACCAGCGCATGCCCGGCGAGTACGGCAATGAACTGCTGCGCCATGCGCGCGAG
CGCTATCCGCATATCGTGCGGATCCTGACCACGGCATACTCGGAACTCGACCAGACCGTGGAAGCGGTCAACCAGGGCCA
GATCCACCGCTACATCAAAAAGCCGTGGGACATCACCGCGCTGCGCATGGAACTGAAGCAGGCGCTGGAGATGGCCGGCC
TGCGCAAGGAGCGCGACCAACTCGTGCGCGAAAAGCTGAGCGTGCTGCAGACGCAGACCGTGGCAACGCGCATCGGGATG
ATTCACGCGCTGTGCGCGAGCCTGATTGGCCCTGGCCGTTTCCAGCCCGTGGAAACCTACCTGGCCGCAACCGAATTGGC
GGGCGCGCGCAATGCCGAGCCTGACTGGCAGCGTATGGACTACGCCGACCTTGTGCGCGCCGAGAGCGAACGCAGCGGCA
GCTTCGGCCATGCGGTAGCGACGCAGCTTGCGGGTTTGCGCGGACGCTATGCGGGGCGCGGCGCCGCGGACGCGGTACAG
GTCATTGCCGAATCGCTCGGCGATGGTACCGTGCGCCGCGACGGCGAAGCGGTGGTATGGACCTCGCCGTCTGCGCTCAG
CGAGTTCGTGGCGAAGCCGGTTGGTGAAGCGGTGTCGGCCCAGCATGCGACATGGCTGGCGTGCCTGCTGTGGCTCGAGG
AAGTCGGTGGCGCGCTGCGCTTCGCGCGAGAAGGCGATGCCATTGCGTGCCGTGTCGGCGAACGCGCGGCCAGCTTTTCG
ACGGACCGTCTGGCGATCTGGATCGAGCGGTTTTCTGAATTGGCTTGA

Upstream 100 bases:

>100_bases
CGTTTGGCGGCGGCATCCGGATCGCCTCAGCGTCCGGAGCCGGCACGACGGTGACGCTGGACTTCCCAAATTTCAAGAAT
CGAATGCACAGGAGTGACCG

Downstream 100 bases:

>100_bases
GCGACTTGCTACCGCGGGCAACAAAAAAGCGCACCGGCTGGTGCGCTTTTTTATTTGGACATCAGGCCGGCTTCAGGCCT
GCGCCCCGAAGTTCGGATCA

Product: response regulator receiver

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSEPNATQAPPPAILFVDDEATAVKYFHRAIGALAPVVTAGSVEEGKTLLEAHADSLAVLVSDQRMPGEYGNELLRHARE
RYPHIVRILTTAYSELDQTVEAVNQGQIHRYIKKPWDITALRMELKQALEMAGLRKERDQLVREKLSVLQTQTVATRIGM
IHALCASLIGPGRFQPVETYLAATELAGARNAEPDWQRMDYADLVRAESERSGSFGHAVATQLAGLRGRYAGRGAADAVQ
VIAESLGDGTVRRDGEAVVWTSPSALSEFVAKPVGEAVSAQHATWLACLLWLEEVGGALRFAREGDAIACRVGERAASFS
TDRLAIWIERFSELA

Sequences:

>Translated_335_residues
MSEPNATQAPPPAILFVDDEATAVKYFHRAIGALAPVVTAGSVEEGKTLLEAHADSLAVLVSDQRMPGEYGNELLRHARE
RYPHIVRILTTAYSELDQTVEAVNQGQIHRYIKKPWDITALRMELKQALEMAGLRKERDQLVREKLSVLQTQTVATRIGM
IHALCASLIGPGRFQPVETYLAATELAGARNAEPDWQRMDYADLVRAESERSGSFGHAVATQLAGLRGRYAGRGAADAVQ
VIAESLGDGTVRRDGEAVVWTSPSALSEFVAKPVGEAVSAQHATWLACLLWLEEVGGALRFAREGDAIACRVGERAASFS
TDRLAIWIERFSELA
>Mature_334_residues
SEPNATQAPPPAILFVDDEATAVKYFHRAIGALAPVVTAGSVEEGKTLLEAHADSLAVLVSDQRMPGEYGNELLRHARER
YPHIVRILTTAYSELDQTVEAVNQGQIHRYIKKPWDITALRMELKQALEMAGLRKERDQLVREKLSVLQTQTVATRIGMI
HALCASLIGPGRFQPVETYLAATELAGARNAEPDWQRMDYADLVRAESERSGSFGHAVATQLAGLRGRYAGRGAADAVQV
IAESLGDGTVRRDGEAVVWTSPSALSEFVAKPVGEAVSAQHATWLACLLWLEEVGGALRFAREGDAIACRVGERAASFST
DRLAIWIERFSELA

Specific function: Probable member of the two-component regulatory system involved in the regulation of the hydrogenase activity. HoxA is probably phosphorylated by a sensory component (which could be hoxX) and then acts in conjunction with sigma-54 as a transcriptional act

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=103, Percent_Identity=38.8349514563107, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 36529; Mature: 36398

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPNATQAPPPAILFVDDEATAVKYFHRAIGALAPVVTAGSVEEGKTLLEAHADSLAVL
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEE
VSDQRMPGEYGNELLRHARERYPHIVRILTTAYSELDQTVEAVNQGQIHRYIKKPWDITA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LRMELKQALEMAGLRKERDQLVREKLSVLQTQTVATRIGMIHALCASLIGPGRFQPVETY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LAATELAGARNAEPDWQRMDYADLVRAESERSGSFGHAVATQLAGLRGRYAGRGAADAVQ
HHHHHHCCCCCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
VIAESLGDGTVRRDGEAVVWTSPSALSEFVAKPVGEAVSAQHATWLACLLWLEEVGGALR
HHHHHHCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAREGDAIACRVGERAASFSTDRLAIWIERFSELA
HHCCCCEEEEECCHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEPNATQAPPPAILFVDDEATAVKYFHRAIGALAPVVTAGSVEEGKTLLEAHADSLAVL
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEE
VSDQRMPGEYGNELLRHARERYPHIVRILTTAYSELDQTVEAVNQGQIHRYIKKPWDITA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LRMELKQALEMAGLRKERDQLVREKLSVLQTQTVATRIGMIHALCASLIGPGRFQPVETY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LAATELAGARNAEPDWQRMDYADLVRAESERSGSFGHAVATQLAGLRGRYAGRGAADAVQ
HHHHHHCCCCCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
VIAESLGDGTVRRDGEAVVWTSPSALSEFVAKPVGEAVSAQHATWLACLLWLEEVGGALR
HHHHHHCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAREGDAIACRVGERAASFSTDRLAIWIERFSELA
HHCCCCEEEEECCHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2001989; 12948488 [H]