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The map label for this gene is hupR1 [H]

Identifier: 73542498

GI number: 73542498

Start: 3084055

End: 3085188

Strand: Reverse

Name: hupR1 [H]

Synonym: Reut_A2813

Alternate gene names: 73542498

Gene position: 3085188-3084055 (Counterclockwise)

Preceding gene: 73542499

Following gene: 73542497

Centisome position: 81.05

GC content: 66.49

Gene sequence:

>1134_bases
ATGACAGAAGCCCAGCAGGCACGGCCCGCCGTGCTCTACGTCGACGATGAAGATATGGCGCGCAAGTACTTTGCGCGCGC
CGTCGGCAACGAGTACGAAGTGCTGACCGCCGCCGGCGCCGACGAAGCCATCGGCATGCTGCGTGCCGCCGGTACGCGTA
CGGTCATCCTGGTGACCGACTTCCGCATGCCGGGGCGCGACGGCGGGGACCTGCTGCGCCAGGTGGCCGAGGAATTCCCC
CAGATCGTGCGCATCCTCGTCACGGCCTATGCCGACAAGGACGTGCTGCTGCAGACTGTCAATTCCGGCGAGGTGTTCCG
CATCCTGGAGAAGCCGCTCAGCGTCGGCGATGTGCGCAACGTGCTGCGCCTGGCCGTGGAGCGCTCCCGCGAGCGGACCC
TGCGGCAGCAGCGCCTGATGGCCATCGACGAGACGCTGGCGTTCCTGGCCCATGAACTGAACACGCCGCTGGCGGCCATC
GCCAATTTCGCGCGCGGTATCGAAGGCCGCGTGGCAGCCGAATACAGTGCCCAGCGGCAGGGCGAGATCGGACAGGCAGC
CACCGCGATGCACGACAACGCGCAGTATTGCCTCGCGGTGTTGTCGTCGTTCCTGCAATCCGTGCGCAGCAGCGCGGGCA
GCGCGCCGACGCGTCAGGAGCCCGCCGCCGACGTGAGCGCGGGCGCGCTGGTCGCATCGCTGCTCGATACCTATCCGTTT
GCCGGCGATCAGCGCAACTGGGTACAGGTTGAGATGCACGGCGACTTCCCGGTACAGACCTTGCCCAACTGCGTGGCGCT
GGTGCTGTCGTCGGTGATGAGCAATGCGCTGCGCGCACTCGGCGACACCAGTGTGCCAAACCTGCGCTTTGTCGTGGTCG
CGCAGCCGCGGCAGGAGATCCGCATCAGCGACAACGGTCCGGGGATTCCGCCCGAAGTGATGGACCGCCTGCTCGTGGAT
CCCGTCACTACTCACGCGGGGGCCGGCGGCAGTGGCCTCGGCATGATTTTCTGCAACCGCGTCATGCAGTCGTTTGGCGG
CGGCATCCGGATCGCCTCAGCGTCCGGAGCCGGCACGACGGTGACGCTGGACTTCCCAAATTTCAAGAATCGAATGCACA
GGAGTGACCGATGA

Upstream 100 bases:

>100_bases
GCGGTGATCGAACGGCATGGCGGCCAGCTCATCGTCGAGAGCGAATTCGGCCACTGGGCCGCCTTCAGCTTCGACCTGCC
GCGCGCGGAGTGACACGGCC

Downstream 100 bases:

>100_bases
GCGAACCGAATGCCACCCAGGCACCACCCCCGGCGATTCTGTTCGTCGACGACGAAGCCACAGCCGTCAAATACTTCCAT
CGCGCGATCGGCGCCCTGGC

Product: response regulator receiver sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTEAQQARPAVLYVDDEDMARKYFARAVGNEYEVLTAAGADEAIGMLRAAGTRTVILVTDFRMPGRDGGDLLRQVAEEFP
QIVRILVTAYADKDVLLQTVNSGEVFRILEKPLSVGDVRNVLRLAVERSRERTLRQQRLMAIDETLAFLAHELNTPLAAI
ANFARGIEGRVAAEYSAQRQGEIGQAATAMHDNAQYCLAVLSSFLQSVRSSAGSAPTRQEPAADVSAGALVASLLDTYPF
AGDQRNWVQVEMHGDFPVQTLPNCVALVLSSVMSNALRALGDTSVPNLRFVVVAQPRQEIRISDNGPGIPPEVMDRLLVD
PVTTHAGAGGSGLGMIFCNRVMQSFGGGIRIASASGAGTTVTLDFPNFKNRMHRSDR

Sequences:

>Translated_377_residues
MTEAQQARPAVLYVDDEDMARKYFARAVGNEYEVLTAAGADEAIGMLRAAGTRTVILVTDFRMPGRDGGDLLRQVAEEFP
QIVRILVTAYADKDVLLQTVNSGEVFRILEKPLSVGDVRNVLRLAVERSRERTLRQQRLMAIDETLAFLAHELNTPLAAI
ANFARGIEGRVAAEYSAQRQGEIGQAATAMHDNAQYCLAVLSSFLQSVRSSAGSAPTRQEPAADVSAGALVASLLDTYPF
AGDQRNWVQVEMHGDFPVQTLPNCVALVLSSVMSNALRALGDTSVPNLRFVVVAQPRQEIRISDNGPGIPPEVMDRLLVD
PVTTHAGAGGSGLGMIFCNRVMQSFGGGIRIASASGAGTTVTLDFPNFKNRMHRSDR
>Mature_376_residues
TEAQQARPAVLYVDDEDMARKYFARAVGNEYEVLTAAGADEAIGMLRAAGTRTVILVTDFRMPGRDGGDLLRQVAEEFPQ
IVRILVTAYADKDVLLQTVNSGEVFRILEKPLSVGDVRNVLRLAVERSRERTLRQQRLMAIDETLAFLAHELNTPLAAIA
NFARGIEGRVAAEYSAQRQGEIGQAATAMHDNAQYCLAVLSSFLQSVRSSAGSAPTRQEPAADVSAGALVASLLDTYPFA
GDQRNWVQVEMHGDFPVQTLPNCVALVLSSVMSNALRALGDTSVPNLRFVVVAQPRQEIRISDNGPGIPPEVMDRLLVDP
VTTHAGAGGSGLGMIFCNRVMQSFGGGIRIASASGAGTTVTLDFPNFKNRMHRSDR

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=28.2700421940928, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=27.1255060728745, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: 2.7.3.- [C]

Molecular weight: Translated: 40729; Mature: 40598

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAQQARPAVLYVDDEDMARKYFARAVGNEYEVLTAAGADEAIGMLRAAGTRTVILVTD
CCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCEEEEEEE
FRMPGRDGGDLLRQVAEEFPQIVRILVTAYADKDVLLQTVNSGEVFRILEKPLSVGDVRN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCCCHHHHHHHCCCCHHHHHH
VLRLAVERSRERTLRQQRLMAIDETLAFLAHELNTPLAAIANFARGIEGRVAAEYSAQRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHC
GEIGQAATAMHDNAQYCLAVLSSFLQSVRSSAGSAPTRQEPAADVSAGALVASLLDTYPF
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
AGDQRNWVQVEMHGDFPVQTLPNCVALVLSSVMSNALRALGDTSVPNLRFVVVAQPRQEI
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHE
RISDNGPGIPPEVMDRLLVDPVTTHAGAGGSGLGMIFCNRVMQSFGGGIRIASASGAGTT
EECCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCE
VTLDFPNFKNRMHRSDR
EEEECCCHHHHHHCCCC
>Mature Secondary Structure 
TEAQQARPAVLYVDDEDMARKYFARAVGNEYEVLTAAGADEAIGMLRAAGTRTVILVTD
CCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCEEEEEEE
FRMPGRDGGDLLRQVAEEFPQIVRILVTAYADKDVLLQTVNSGEVFRILEKPLSVGDVRN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCCCHHHHHHHCCCCHHHHHH
VLRLAVERSRERTLRQQRLMAIDETLAFLAHELNTPLAAIANFARGIEGRVAAEYSAQRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHC
GEIGQAATAMHDNAQYCLAVLSSFLQSVRSSAGSAPTRQEPAADVSAGALVASLLDTYPF
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
AGDQRNWVQVEMHGDFPVQTLPNCVALVLSSVMSNALRALGDTSVPNLRFVVVAQPRQEI
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHE
RISDNGPGIPPEVMDRLLVDPVTTHAGAGGSGLGMIFCNRVMQSFGGGIRIASASGAGTT
EECCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCE
VTLDFPNFKNRMHRSDR
EEEECCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]