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The map label for this gene is pbuE [H]

Identifier: 73541310

GI number: 73541310

Start: 1742151

End: 1743323

Strand: Direct

Name: pbuE [H]

Synonym: Reut_A1620

Alternate gene names: 73541310

Gene position: 1742151-1743323 (Clockwise)

Preceding gene: 73541309

Following gene: 73541313

Centisome position: 45.77

GC content: 64.11

Gene sequence:

>1173_bases
ATGGATCGTCGACTACTGATGCTGGCCCTCGGCATGTTTGCCATGGGAACGGACAACTTCGTTGTTGCCGGCATTCTTCC
GAACGTCGCTGCATCGCTGCACACGACCCCGAGCCTCGCGGGCCAGATGGTCACCGTCTATGCGCTGTCCTATGCAATCC
TGGCGCCGGTGATGGCGGCTGTCGCGGGGGCGTGGCCAAGAAAGCTGCTGCTGGTCACCGCGCTCGGCATCTTCGTCATT
GGCAATGCCATCACAGCCCTTGCGACGGATTTGAACACGGTCCTGTTCAGTCGCGCGCTGGCGGGCCTTGGCGCGGCGAT
GTTCTCTCCTACCGCATTGGGTGTCGCCACGTTACTGGCGGCTCCTGAGAGGAGAGGGCGCGCGCTTGCCACGGTGACCG
CCGGCCTCGCGGGCGCAACGGCGCTGGGCGCCCCGATCGGCACGTTCATCGGTGGGTTCGGCAGTTGGAGAACCACGCTC
TGGTTCGTCGCGCTCCTGGGGCTGATCGCGATGATCGGGATCGGGGCCATGCTGCGCTCGGTTCCTCAGCCTGCGCGAAT
CTCGCTGCGCGATAGGCTGACGCCCCTGCGCGACGTCCGCGTTGCCCTGACCCTGCTCACGTCCCTGTTTGCGTTCGGCG
GCTTCCTGATGGTCTACACCTATGCCGGACTCGTGCTGGATCGGGTGACCGGCGGCGACGAACGCGTGCTGGCCGGCATG
CTGCTCGTCTGGGGCTGCGCCGCGACAGTCGGGAACATGCTTGCCGGCCGCCTCGTTGACCGCTTCGACAGTCGCAAGAT
CATCAATGCAGGCCTTTGCATCGGCATTCTCAATTTCTGTGCGCTGCCATGGACTTCGGCATACATGGTCACCGCCGCAG
TCTCCTTGGTCGCCTGGGGGCTGGTCGGATGGGGATTGATTGTGCCGCAACAGCACCGGCTGGTAAAAATCGCGCCTCCC
ATGGCGCCGTTGCTGCTGGCCCTTAACAATACGGCTACCTACGGCGGGCTGGCCTGCTCAGGTGTTCTCGGGGGCATTGT
GCTGCTGTTCATCGACCGGCAATACCTCAGTATCGTGGGTGCCGGATTGATCGCGATCGCTCTGGTTTTCGCCGAGGCCG
CGCATCTGTGCTCTGCGCGCCTCTCCACGAGCATGCGACCTATGCCTTCGTGA

Upstream 100 bases:

>100_bases
GGCGGCCCACGGAACACTGACTCAGCAGCTTTGGGAAAGGCCTTGGTAGCGCACCCACTTCCCAATCCACGTAAACGTCA
TTCCAGTAAAGGTCATTCAA

Downstream 100 bases:

>100_bases
AGATCTCCAAAAAATGCTGGCTCGGATCCTCGAAGTAAATCCGGCGGCCGCCACCGTCCGTGTTGACCTCGCCGGGGCGG
CGACGGTAGGGGTCCGCCCA

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MDRRLLMLALGMFAMGTDNFVVAGILPNVAASLHTTPSLAGQMVTVYALSYAILAPVMAAVAGAWPRKLLLVTALGIFVI
GNAITALATDLNTVLFSRALAGLGAAMFSPTALGVATLLAAPERRGRALATVTAGLAGATALGAPIGTFIGGFGSWRTTL
WFVALLGLIAMIGIGAMLRSVPQPARISLRDRLTPLRDVRVALTLLTSLFAFGGFLMVYTYAGLVLDRVTGGDERVLAGM
LLVWGCAATVGNMLAGRLVDRFDSRKIINAGLCIGILNFCALPWTSAYMVTAAVSLVAWGLVGWGLIVPQQHRLVKIAPP
MAPLLLALNNTATYGGLACSGVLGGIVLLFIDRQYLSIVGAGLIAIALVFAEAAHLCSARLSTSMRPMPS

Sequences:

>Translated_390_residues
MDRRLLMLALGMFAMGTDNFVVAGILPNVAASLHTTPSLAGQMVTVYALSYAILAPVMAAVAGAWPRKLLLVTALGIFVI
GNAITALATDLNTVLFSRALAGLGAAMFSPTALGVATLLAAPERRGRALATVTAGLAGATALGAPIGTFIGGFGSWRTTL
WFVALLGLIAMIGIGAMLRSVPQPARISLRDRLTPLRDVRVALTLLTSLFAFGGFLMVYTYAGLVLDRVTGGDERVLAGM
LLVWGCAATVGNMLAGRLVDRFDSRKIINAGLCIGILNFCALPWTSAYMVTAAVSLVAWGLVGWGLIVPQQHRLVKIAPP
MAPLLLALNNTATYGGLACSGVLGGIVLLFIDRQYLSIVGAGLIAIALVFAEAAHLCSARLSTSMRPMPS
>Mature_390_residues
MDRRLLMLALGMFAMGTDNFVVAGILPNVAASLHTTPSLAGQMVTVYALSYAILAPVMAAVAGAWPRKLLLVTALGIFVI
GNAITALATDLNTVLFSRALAGLGAAMFSPTALGVATLLAAPERRGRALATVTAGLAGATALGAPIGTFIGGFGSWRTTL
WFVALLGLIAMIGIGAMLRSVPQPARISLRDRLTPLRDVRVALTLLTSLFAFGGFLMVYTYAGLVLDRVTGGDERVLAGM
LLVWGCAATVGNMLAGRLVDRFDSRKIINAGLCIGILNFCALPWTSAYMVTAAVSLVAWGLVGWGLIVPQQHRLVKIAPP
MAPLLLALNNTATYGGLACSGVLGGIVLLFIDRQYLSIVGAGLIAIALVFAEAAHLCSARLSTSMRPMPS

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=321, Percent_Identity=29.2834890965732, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786595, Length=267, Percent_Identity=25.8426966292135, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787808, Length=175, Percent_Identity=27.4285714285714, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40564; Mature: 40564

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRLLMLALGMFAMGTDNFVVAGILPNVAASLHTTPSLAGQMVTVYALSYAILAPVMAA
CCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VAGAWPRKLLLVTALGIFVIGNAITALATDLNTVLFSRALAGLGAAMFSPTALGVATLLA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
APERRGRALATVTAGLAGATALGAPIGTFIGGFGSWRTTLWFVALLGLIAMIGIGAMLRS
CCHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
VPQPARISLRDRLTPLRDVRVALTLLTSLFAFGGFLMVYTYAGLVLDRVTGGDERVLAGM
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LLVWGCAATVGNMLAGRLVDRFDSRKIINAGLCIGILNFCALPWTSAYMVTAAVSLVAWG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVGWGLIVPQQHRLVKIAPPMAPLLLALNNTATYGGLACSGVLGGIVLLFIDRQYLSIVG
HHHHHHCCCCCCCEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGLIAIALVFAEAAHLCSARLSTSMRPMPS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDRRLLMLALGMFAMGTDNFVVAGILPNVAASLHTTPSLAGQMVTVYALSYAILAPVMAA
CCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VAGAWPRKLLLVTALGIFVIGNAITALATDLNTVLFSRALAGLGAAMFSPTALGVATLLA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
APERRGRALATVTAGLAGATALGAPIGTFIGGFGSWRTTLWFVALLGLIAMIGIGAMLRS
CCHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
VPQPARISLRDRLTPLRDVRVALTLLTSLFAFGGFLMVYTYAGLVLDRVTGGDERVLAGM
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LLVWGCAATVGNMLAGRLVDRFDSRKIINAGLCIGILNFCALPWTSAYMVTAAVSLVAWG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVGWGLIVPQQHRLVKIAPPMAPLLLALNNTATYGGLACSGVLGGIVLLFIDRQYLSIVG
HHHHHHCCCCCCCEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGLIAIALVFAEAAHLCSARLSTSMRPMPS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]