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The map label for this gene is bam [H]

Identifier: 73541309

GI number: 73541309

Start: 1740642

End: 1742099

Strand: Direct

Name: bam [H]

Synonym: Reut_A1619

Alternate gene names: 73541309

Gene position: 1740642-1742099 (Clockwise)

Preceding gene: 73541308

Following gene: 73541310

Centisome position: 45.73

GC content: 62.55

Gene sequence:

>1458_bases
ATGTCAGCGAATCCAATGCAAAGTAGCCACTGTACGATCGACGCACCCGAGAACGATCTCTGGCGCTGGTCCGGCGCCGA
AATTGCGACTGCGGTTTGTCGAGGGGATGTTTCGGCCGTCGAAGTGGTGGAAAGCGTCTACGCGCGTATCGCCGCAGTCA
ATCCCATGATCAACGGCATTGTCCATATGGACAGGGAGCACGCGTTGACGGATGCCGGTGCGCTCGACCGGCGGCGCCAG
GCCGGCGATCCACTGGGGCCGCTTGCCGGCGTGCCGGTCTCGATCAAGCTTAACGTTGACGTCGCCGGTGAGGCGACAAG
CAATGGAAATCCGCTGTGGCTTGATCGCATTGCTGAAGCGGACAGTGGCGTGGTTTCCAACCTGCGCCGTGCGGGGGCCG
TGGTCATAGGACGGACAAATGCTCCGCCGCTTTCTTTCCGCTGGTTCACGGAAAACCCCATTTATGGACGAACTCATAAC
CCATGGTCGCCGGCAGTCACCTCGGGCGGCTCAAGCGGGGGCGCCGGTGCTGCGGTAGCGAGCGGGATGGGGCCGATCGC
GCATGGCACTGACATTGCGGGATCCATCCGCTATCCGGCGTTTGTCAACGGTGTCGTCGGCTTGCGAGCAACTACGGGAC
GCATCCCCGGATTCCAGGCCACTGCGCCACAGCGCTTCTTTGGACTTCAGGCGATGTCTGCGCAGGGCCCAATCGCACGC
ACGGTTTCGGATGCGCGGCTCGGACTGGAGGCGATGGCCAGCGACGGCGGCATTGACCCGGTCTGGGTCGATGCCCGCCT
GAATTACGATGACGATGAGACGCCAACACGCGTTGCGCTGGTAGACGAGATCCGTGGCATCGATATCAGCCCGGAGATTC
AACAAGCGCTCTCATCGGCCGCCGCGGCGCTGATCGATGCAGGTTACACGGTCGAACGCGTCGCGTTGCCAGACCTCAAG
CGCGGGATGGAGTTGTGGGAAAGCATCGTCATGTCCGAATGCAACCTGGGCATGCTTGCCGCCGTGGATGCAATTGGCGA
CGCGACCATTAGTACGCCGGTCCGCAACATGGCCGCCTGCGCGCCTGAGCCAAGCCTGGCTGGGTATGCCGCAGCAATCG
CTTCACGCGATGAAATGCGCCGGAGCTGGAATATCTTGTTCCAGCGCCATCCGCTGGTGCTCATGCCTACCTCGTGTCGC
CAGCCACTGCCGTGGGGCGCAGATCAGGGCTCAGTCGATGAAATGCGTCGCTTGCTGGAAATACAGAGCCCGCTTATGGC
GGTTGCCGTGTGTGGGTTGCCAGGGCTGCACGTCCCGACAGGGCTGTCACAGGGATTGCCTGTCGGCGTCCAGCTCGTTG
CACCGGCATTCCGCGAAATGCGGCTGTTGCACGCGGGCGCCATGATCGAGGCGGCCCACGGAACACTGACTCAGCAGCTT
TGGGAAAGGCCTTGGTAG

Upstream 100 bases:

>100_bases
TGCAGACTTGATCCGCGAGCACGATGCGGGTTGCTCGCTCTGGTGTCCATCAATGTCCGCAACGATTCTCACGTTACCAG
CGGACACGAGATAACAAATA

Downstream 100 bases:

>100_bases
CGCACCCACTTCCCAATCCACGTAAACGTCATTCCAGTAAAGGTCATTCAAATGGATCGTCGACTACTGATGCTGGCCCT
CGGCATGTTTGCCATGGGAA

Product: amidase

Products: NA

Alternate protein names: IAH; Indole-3-acetamide hydrolase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSANPMQSSHCTIDAPENDLWRWSGAEIATAVCRGDVSAVEVVESVYARIAAVNPMINGIVHMDREHALTDAGALDRRRQ
AGDPLGPLAGVPVSIKLNVDVAGEATSNGNPLWLDRIAEADSGVVSNLRRAGAVVIGRTNAPPLSFRWFTENPIYGRTHN
PWSPAVTSGGSSGGAGAAVASGMGPIAHGTDIAGSIRYPAFVNGVVGLRATTGRIPGFQATAPQRFFGLQAMSAQGPIAR
TVSDARLGLEAMASDGGIDPVWVDARLNYDDDETPTRVALVDEIRGIDISPEIQQALSSAAAALIDAGYTVERVALPDLK
RGMELWESIVMSECNLGMLAAVDAIGDATISTPVRNMAACAPEPSLAGYAAAIASRDEMRRSWNILFQRHPLVLMPTSCR
QPLPWGADQGSVDEMRRLLEIQSPLMAVAVCGLPGLHVPTGLSQGLPVGVQLVAPAFREMRLLHAGAMIEAAHGTLTQQL
WERPW

Sequences:

>Translated_485_residues
MSANPMQSSHCTIDAPENDLWRWSGAEIATAVCRGDVSAVEVVESVYARIAAVNPMINGIVHMDREHALTDAGALDRRRQ
AGDPLGPLAGVPVSIKLNVDVAGEATSNGNPLWLDRIAEADSGVVSNLRRAGAVVIGRTNAPPLSFRWFTENPIYGRTHN
PWSPAVTSGGSSGGAGAAVASGMGPIAHGTDIAGSIRYPAFVNGVVGLRATTGRIPGFQATAPQRFFGLQAMSAQGPIAR
TVSDARLGLEAMASDGGIDPVWVDARLNYDDDETPTRVALVDEIRGIDISPEIQQALSSAAAALIDAGYTVERVALPDLK
RGMELWESIVMSECNLGMLAAVDAIGDATISTPVRNMAACAPEPSLAGYAAAIASRDEMRRSWNILFQRHPLVLMPTSCR
QPLPWGADQGSVDEMRRLLEIQSPLMAVAVCGLPGLHVPTGLSQGLPVGVQLVAPAFREMRLLHAGAMIEAAHGTLTQQL
WERPW
>Mature_484_residues
SANPMQSSHCTIDAPENDLWRWSGAEIATAVCRGDVSAVEVVESVYARIAAVNPMINGIVHMDREHALTDAGALDRRRQA
GDPLGPLAGVPVSIKLNVDVAGEATSNGNPLWLDRIAEADSGVVSNLRRAGAVVIGRTNAPPLSFRWFTENPIYGRTHNP
WSPAVTSGGSSGGAGAAVASGMGPIAHGTDIAGSIRYPAFVNGVVGLRATTGRIPGFQATAPQRFFGLQAMSAQGPIART
VSDARLGLEAMASDGGIDPVWVDARLNYDDDETPTRVALVDEIRGIDISPEIQQALSSAAAALIDAGYTVERVALPDLKR
GMELWESIVMSECNLGMLAAVDAIGDATISTPVRNMAACAPEPSLAGYAAAIASRDEMRRSWNILFQRHPLVLMPTSCRQ
PLPWGADQGSVDEMRRLLEIQSPLMAVAVCGLPGLHVPTGLSQGLPVGVQLVAPAFREMRLLHAGAMIEAAHGTLTQQLW
ERPW

Specific function: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid (IAA) [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=487, Percent_Identity=27.9260780287474, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=23.7424547283702, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI166795287, Length=233, Percent_Identity=34.7639484978541, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=510, Percent_Identity=28.6274509803922, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=298, Percent_Identity=30.8724832214765, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17538252, Length=314, Percent_Identity=28.343949044586, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=301, Percent_Identity=28.9036544850498, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=203, Percent_Identity=33.0049261083744, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=203, Percent_Identity=33.0049261083744, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=297, Percent_Identity=27.6094276094276, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=241, Percent_Identity=30.2904564315353, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319685, Length=161, Percent_Identity=37.2670807453416, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652985, Length=506, Percent_Identity=25.4940711462451, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922090, Length=506, Percent_Identity=25.4940711462451, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24652981, Length=506, Percent_Identity=25.4940711462451, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24652983, Length=506, Percent_Identity=25.4940711462451, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24644968, Length=500, Percent_Identity=27.2, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24648435, Length=461, Percent_Identity=26.6811279826464, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24648437, Length=461, Percent_Identity=26.6811279826464, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI45550774, Length=468, Percent_Identity=25.8547008547009, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24648441, Length=429, Percent_Identity=25.4079254079254, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648439, Length=429, Percent_Identity=25.4079254079254, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648113, Length=495, Percent_Identity=24.8484848484848, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356731, Length=232, Percent_Identity=29.3103448275862, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI161078093, Length=518, Percent_Identity=24.1312741312741, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 51288; Mature: 51157

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANPMQSSHCTIDAPENDLWRWSGAEIATAVCRGDVSAVEVVESVYARIAAVNPMINGI
CCCCCCCCCCEEEECCCCCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
VHMDREHALTDAGALDRRRQAGDPLGPLAGVPVSIKLNVDVAGEATSNGNPLWLDRIAEA
HHCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEHHHHHCC
DSGVVSNLRRAGAVVIGRTNAPPLSFRWFTENPIYGRTHNPWSPAVTSGGSSGGAGAAVA
CCHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
SGMGPIAHGTDIAGSIRYPAFVNGVVGLRATTGRIPGFQATAPQRFFGLQAMSAQGPIAR
CCCCCCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCHHH
TVSDARLGLEAMASDGGIDPVWVDARLNYDDDETPTRVALVDEIRGIDISPEIQQALSSA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHHH
AAALIDAGYTVERVALPDLKRGMELWESIVMSECNLGMLAAVDAIGDATISTPVRNMAAC
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHC
APEPSLAGYAAAIASRDEMRRSWNILFQRHPLVLMPTSCRQPLPWGADQGSVDEMRRLLE
CCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
IQSPLMAVAVCGLPGLHVPTGLSQGLPVGVQLVAPAFREMRLLHAGAMIEAAHGTLTQQL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
WERPW
HCCCC
>Mature Secondary Structure 
SANPMQSSHCTIDAPENDLWRWSGAEIATAVCRGDVSAVEVVESVYARIAAVNPMINGI
CCCCCCCCCEEEECCCCCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
VHMDREHALTDAGALDRRRQAGDPLGPLAGVPVSIKLNVDVAGEATSNGNPLWLDRIAEA
HHCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEHHHHHCC
DSGVVSNLRRAGAVVIGRTNAPPLSFRWFTENPIYGRTHNPWSPAVTSGGSSGGAGAAVA
CCHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
SGMGPIAHGTDIAGSIRYPAFVNGVVGLRATTGRIPGFQATAPQRFFGLQAMSAQGPIAR
CCCCCCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCHHH
TVSDARLGLEAMASDGGIDPVWVDARLNYDDDETPTRVALVDEIRGIDISPEIQQALSSA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHHH
AAALIDAGYTVERVALPDLKRGMELWESIVMSECNLGMLAAVDAIGDATISTPVRNMAAC
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHC
APEPSLAGYAAAIASRDEMRRSWNILFQRHPLVLMPTSCRQPLPWGADQGSVDEMRRLLE
CCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
IQSPLMAVAVCGLPGLHVPTGLSQGLPVGVQLVAPAFREMRLLHAGAMIEAAHGTLTQQL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
WERPW
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]