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The map label for this gene is guaA

Identifier: 71907956

GI number: 71907956

Start: 2518639

End: 2520273

Strand: Reverse

Name: guaA

Synonym: Daro_2337

Alternate gene names: 71907956

Gene position: 2520273-2518639 (Counterclockwise)

Preceding gene: 71907957

Following gene: 71907955

Centisome position: 55.99

GC content: 59.63

Gene sequence:

>1635_bases
ATGTCTCACCAGAAAATCCTCATCCTCGATTTCGGTTCCCAGGTCACTCAGCTGATCGCCCGTCGCGTGCGCGAAGCGAA
GGTCTTCTGCGAAATTCATCCGTACGACGTTTCCGAAGAATTTATTCGCAATTACGGCGCCAAGGGCATCATCTTGTCGG
GTGGCCCAAGTTCGGTGACCGAGGGTGATACGCCGCGAGCGCCGGAAGTGGTTTTCCAACTCGGTGTTCCGGTTCTCGGC
ATTTGCTACGGCATGCAGACCATGGCCCAGCAACTGGGTGGCAAGGTGATGACCGCCGAGGCAGCGGGCAAATCCCGCGA
ATTTGGCTACTCGGAAGTCCGCGCCCACGGCCACACGGCACTGCTCAACGATATTGCCGACTTTTATAGTCCGGAAGGTC
ACGGCATGCTCAAGGTCTGGATGAGCCACGGCGATTCGGTCATGGAACTGCCGGCCGGCTTCGTCAAGATGGCTTCGACC
GCCTCGTGCCCGATTGCGGCGATGGCCGACGAGAGCCGCAAGTTCTACGCTGTTCAGTTCCACCCGGAAGTGACGCACAC
CCAGCAGGGCCGCGCCATCCTCGAACGTTTCGTGCATGGCATCTGCGGCTGTGGCACTGACTGGGTCATGGGCGACTACA
TCGCCGAGGCGGTCGATGCCATCCGGCGTCAGGTCGGCGACGAGGAAGTCATTCTCGGCCTGTCCGGCGGTGTCGATTCC
AGTGTTGCCGCGGCGCTGATCCACAAGGCAATCGGCGACCAGCTGACCTGCGTTTTTGTCGATCACGGCCTGCTACGCCT
GAACGAAGGCGACATGGTGATGGAGATGTTTGCCCGTAATCTTGGCGTCAAGGTCATCCGTGTAGATGCCGTTGATGACT
TCATGGGCAAGCTGGCCGGTGTCTCCGACCCCGAGCAGAAGCGCAAGATCATCGGTAAGGAATTCGTGGAAGTTTTCCAG
GTTGAATCCAAAAAGCTTGCCGCTGCCAAGTGGCTGGCCCAGGGCACGATCTACCCGGACGTGATCGAGTCGGCCGGCAA
GGGCAAGAAGGGCGCTCACACGATCAAGAGCCACCATAACGTTGGCGGCCTGCCGGAAGATATGCACCTCAAATTGCTTG
AGCCGCTGCGTGAGTTGTTCAAGGACGAAGTCCGTGAACTGGGCATTGCCCTCGGACTGCCACGCGAGATGGTGTATCGC
CACCCGTTCCCGGGGCCGGGCTTAGGGGTTCGGATTCTTGGCGAAGTCACGCTGAAGGCGGCTCATCTGTTGCAACGCGC
TGATGCCATTTTCATCGATGAACTGCGTGCCACCCACGCAACCGAACGTGATGTGGCGGCCGGTGCCTGCACTGAAGCCG
ACATCGGCAAGAGCTGGTACGACCTGACCAGCCAGGCCTTTGCCGTTTTCCTGCCAGTCAAGAGCGTTGGCGTGATGGGG
GATGGTCGGACCTACGAGAATGTCGTCGCCTTGCGCGCCGTGGTGACCAGCGACTTCATGACGGCACACTGGGCGCACCT
TCCGTATGAGCTGCTTGGCCGGGTTTCCAATCGCATCATCAACGAAGTCCGGGGTCTGAACCGCGTTGTTTATGACGTGT
CAGGCAAGCCGCCGGCTACGATCGAGTGGGAATAA

Upstream 100 bases:

>100_bases
CAGATCACCAAGGAAGCACCGAACTACCATCTCGACTGATCGTTCCTGTGTCCCAGAGGGGCGGCTCCGGTAGCCGCCCT
TTTCATTCAAGGTCTTGAAT

Downstream 100 bases:

>100_bases
TTTATCATCATTTCAAGTCGTTTCACACCGTCCAGATTTTGTAAAATATACCCGCTATAACAACCAGTTAGCGTCTCATG
GCGTCTCACGCCGTCCTAGC

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSHQKILILDFGSQVTQLIARRVREAKVFCEIHPYDVSEEFIRNYGAKGIILSGGPSSVTEGDTPRAPEVVFQLGVPVLG
ICYGMQTMAQQLGGKVMTAEAAGKSREFGYSEVRAHGHTALLNDIADFYSPEGHGMLKVWMSHGDSVMELPAGFVKMAST
ASCPIAAMADESRKFYAVQFHPEVTHTQQGRAILERFVHGICGCGTDWVMGDYIAEAVDAIRRQVGDEEVILGLSGGVDS
SVAAALIHKAIGDQLTCVFVDHGLLRLNEGDMVMEMFARNLGVKVIRVDAVDDFMGKLAGVSDPEQKRKIIGKEFVEVFQ
VESKKLAAAKWLAQGTIYPDVIESAGKGKKGAHTIKSHHNVGGLPEDMHLKLLEPLRELFKDEVRELGIALGLPREMVYR
HPFPGPGLGVRILGEVTLKAAHLLQRADAIFIDELRATHATERDVAAGACTEADIGKSWYDLTSQAFAVFLPVKSVGVMG
DGRTYENVVALRAVVTSDFMTAHWAHLPYELLGRVSNRIINEVRGLNRVVYDVSGKPPATIEWE

Sequences:

>Translated_544_residues
MSHQKILILDFGSQVTQLIARRVREAKVFCEIHPYDVSEEFIRNYGAKGIILSGGPSSVTEGDTPRAPEVVFQLGVPVLG
ICYGMQTMAQQLGGKVMTAEAAGKSREFGYSEVRAHGHTALLNDIADFYSPEGHGMLKVWMSHGDSVMELPAGFVKMAST
ASCPIAAMADESRKFYAVQFHPEVTHTQQGRAILERFVHGICGCGTDWVMGDYIAEAVDAIRRQVGDEEVILGLSGGVDS
SVAAALIHKAIGDQLTCVFVDHGLLRLNEGDMVMEMFARNLGVKVIRVDAVDDFMGKLAGVSDPEQKRKIIGKEFVEVFQ
VESKKLAAAKWLAQGTIYPDVIESAGKGKKGAHTIKSHHNVGGLPEDMHLKLLEPLRELFKDEVRELGIALGLPREMVYR
HPFPGPGLGVRILGEVTLKAAHLLQRADAIFIDELRATHATERDVAAGACTEADIGKSWYDLTSQAFAVFLPVKSVGVMG
DGRTYENVVALRAVVTSDFMTAHWAHLPYELLGRVSNRIINEVRGLNRVVYDVSGKPPATIEWE
>Mature_543_residues
SHQKILILDFGSQVTQLIARRVREAKVFCEIHPYDVSEEFIRNYGAKGIILSGGPSSVTEGDTPRAPEVVFQLGVPVLGI
CYGMQTMAQQLGGKVMTAEAAGKSREFGYSEVRAHGHTALLNDIADFYSPEGHGMLKVWMSHGDSVMELPAGFVKMASTA
SCPIAAMADESRKFYAVQFHPEVTHTQQGRAILERFVHGICGCGTDWVMGDYIAEAVDAIRRQVGDEEVILGLSGGVDSS
VAAALIHKAIGDQLTCVFVDHGLLRLNEGDMVMEMFARNLGVKVIRVDAVDDFMGKLAGVSDPEQKRKIIGKEFVEVFQV
ESKKLAAAKWLAQGTIYPDVIESAGKGKKGAHTIKSHHNVGGLPEDMHLKLLEPLRELFKDEVRELGIALGLPREMVYRH
PFPGPGLGVRILGEVTLKAAHLLQRADAIFIDELRATHATERDVAAGACTEADIGKSWYDLTSQAFAVFLPVKSVGVMGD
GRTYENVVALRAVVTSDFMTAHWAHLPYELLGRVSNRIINEVRGLNRVVYDVSGKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=570, Percent_Identity=35.0877192982456, Blast_Score=284, Evalue=1e-76,
Organism=Escherichia coli, GI1788854, Length=541, Percent_Identity=61.9223659889094, Blast_Score=681, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71992717, Length=582, Percent_Identity=35.5670103092784, Blast_Score=288, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI133901714, Length=582, Percent_Identity=35.5670103092784, Blast_Score=288, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI71992710, Length=582, Percent_Identity=35.5670103092784, Blast_Score=288, Evalue=6e-78,
Organism=Saccharomyces cerevisiae, GI6323873, Length=547, Percent_Identity=46.0694698354662, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI281365319, Length=567, Percent_Identity=35.626102292769, Blast_Score=299, Evalue=4e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_DECAR (Q47DK8)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285543.1
- ProteinModelPortal:   Q47DK8
- SMR:   Q47DK8
- STRING:   Q47DK8
- GeneID:   3566056
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_2337
- NMPDR:   fig|159087.4.peg.2314
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- ProtClustDB:   PRK00074
- BioCyc:   DARO159087:DARO_2337-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 59430; Mature: 59299

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 82-82 ACT_SITE 181-181 ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQKILILDFGSQVTQLIARRVREAKVFCEIHPYDVSEEFIRNYGAKGIILSGGPSSVT
CCCCEEEEEECCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCC
EGDTPRAPEVVFQLGVPVLGICYGMQTMAQQLGGKVMTAEAAGKSREFGYSEVRAHGHTA
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHCCHHH
LLNDIADFYSPEGHGMLKVWMSHGDSVMELPAGFVKMASTASCPIAAMADESRKFYAVQF
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHCHHHHHHHCCCCCCEEEECCCCCEEEEEEE
HPEVTHTQQGRAILERFVHGICGCGTDWVMGDYIAEAVDAIRRQVGDEEVILGLSGGVDS
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCH
SVAAALIHKAIGDQLTCVFVDHGLLRLNEGDMVMEMFARNLGVKVIRVDAVDDFMGKLAG
HHHHHHHHHHHCCCEEEEEEECCEEEECCCHHHHHHHHHHCCEEEEEEECHHHHHHHHCC
VSDPEQKRKIIGKEFVEVFQVESKKLAAAKWLAQGTIYPDVIESAGKGKKGAHTIKSHHN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHCCC
VGGLPEDMHLKLLEPLRELFKDEVRELGIALGLPREMVYRHPFPGPGLGVRILGEVTLKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEHHHHHHHH
AHLLQRADAIFIDELRATHATERDVAAGACTEADIGKSWYDLTSQAFAVFLPVKSVGVMG
HHHHHHHCHHHHHHHHHHCCCHHHHHCCCCCCHHCCCHHHHHHHHHEEEEEEHHHHCCCC
DGRTYENVVALRAVVTSDFMTAHWAHLPYELLGRVSNRIINEVRGLNRVVYDVSGKPPAT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCC
IEWE
CCCC
>Mature Secondary Structure 
SHQKILILDFGSQVTQLIARRVREAKVFCEIHPYDVSEEFIRNYGAKGIILSGGPSSVT
CCCEEEEEECCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCC
EGDTPRAPEVVFQLGVPVLGICYGMQTMAQQLGGKVMTAEAAGKSREFGYSEVRAHGHTA
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHCCHHH
LLNDIADFYSPEGHGMLKVWMSHGDSVMELPAGFVKMASTASCPIAAMADESRKFYAVQF
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHCHHHHHHHCCCCCCEEEECCCCCEEEEEEE
HPEVTHTQQGRAILERFVHGICGCGTDWVMGDYIAEAVDAIRRQVGDEEVILGLSGGVDS
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCH
SVAAALIHKAIGDQLTCVFVDHGLLRLNEGDMVMEMFARNLGVKVIRVDAVDDFMGKLAG
HHHHHHHHHHHCCCEEEEEEECCEEEECCCHHHHHHHHHHCCEEEEEEECHHHHHHHHCC
VSDPEQKRKIIGKEFVEVFQVESKKLAAAKWLAQGTIYPDVIESAGKGKKGAHTIKSHHN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHCCC
VGGLPEDMHLKLLEPLRELFKDEVRELGIALGLPREMVYRHPFPGPGLGVRILGEVTLKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEHHHHHHHH
AHLLQRADAIFIDELRATHATERDVAAGACTEADIGKSWYDLTSQAFAVFLPVKSVGVMG
HHHHHHHCHHHHHHHHHHCCCHHHHHCCCCCCHHCCCHHHHHHHHHEEEEEEHHHHCCCC
DGRTYENVVALRAVVTSDFMTAHWAHLPYELLGRVSNRIINEVRGLNRVVYDVSGKPPAT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCC
IEWE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA