Click here to switch to the map view.

The map label for this gene is pcoS [H]

Identifier: 71907878

GI number: 71907878

Start: 2445131

End: 2446522

Strand: Reverse

Name: pcoS [H]

Synonym: Daro_2258

Alternate gene names: 71907878

Gene position: 2446522-2445131 (Counterclockwise)

Preceding gene: 71907879

Following gene: 71907876

Centisome position: 54.35

GC content: 59.05

Gene sequence:

>1392_bases
GTGAATCTGATCGGGAAAAAATCCGTCACCTTTCGGCTGACCATGCTTTTTGCATCGGTCTCGACCACGGTGCTTCTTAT
GCTGGGACTGCTGATCGGCTCGCTCGTCGAGGGGCATTTCGAAGAACTGGACATGGAACTGCTCGGCGGAAAAATGGAAT
TGCTCCGCCACGCTTTGGAGAAAGTGCGCTCCCCCCAGGAACTGGAAGCGCTGCCGGGACAACTGGAAGATTCGCTGGTC
GGACACCACGGTTTGGCGGTGCGCATCTTGAAAGTGGACGGTCAGCTCCTGTATGCGACGGAAGGATCGACGTTCCCACC
TGGACTGCTTGCCGATGCCGGGAAGCTGCCTCTTCGTCCGACACTCTGGAGCGATTCGGAAAACCATCTCTATCGTGGCA
TCGCCGCCCTGGCACCCACCGGTATCCAGGGCATGGCGCCAGTTGTAGTAGCGGCGTCGACCGACATGACCCACCACGAG
CATTTCATGCACTCATTCAAGGCGGCGCTTTGGTCAGTCGTGGGATTTGCCGCACTTCTCACTGGATTTCTTGGCTGGGT
GGCGGCTCGCCGTGGTCTGGCGCCCCTTCGTGAGATTAGCCGCTCAGCCGCTGGCATTACCGCTAACCGCCTTAACCGGC
GGTTAGCGGTGGAGTCCATTCCCGTCGAACTGGCAGAAGTCGCCCAAACCCTCAATGAGATGCTGGCCCGCCTTGAAGAG
TCCTTCCGCAGGCTGTCTGATTTCTCCTCCGATCTCGCCCACGAACTGCGCACGCCCGTGAGCAATCTGCTGACACAGAC
CCAGGTAACGCTGTCCAAGACGCGGACGCTTGACGAATACCAAGACGTGCTCGCCTCCAACGCCGAAGAGTTCGAGCGCC
TGTCGCGGACCATTGCCGACATGCTATTTTTAGCCAAGTCAGACAACGATCTCATTGTCCCCAATCGAGAAACGGTTGAT
CTGGCGGTCGAGATCGCCAGTCTTTCTGAGTTTTACGAGGCCTGGGTCGAGGAGAAGGGCATTCACCTGACCTGTTCGGG
CGGCGGCCTCGTGCTTGGGGATCGGCTGATGCTGCGTCGGGCCGTCGGCAATCTGCTTTCCAACGCAGTGCGCCACACAC
CGGAAAAAGGAATGATCACGGTCTGCGTGGCCGATGCTGACAACACGTGCATCGTTGTGTCGGTTGAAAATACGGGCGAG
ACCATCGCCCCGGAACATCTGCCCCGTCTGTTTGACCGCTTCTATCGGGTTGATCCGTCCCGGCAACGATTTGGCGAAGG
CGCGGGTCTGGGGCTGGCCATCACACGTTCCATCGCTAGTGCCCATGGCGGTGATGTTCGTGTCCGTTCGGAGAACGGCA
TCACGGCGTTCGAGATTTGGTTGCCGGCCTGA

Upstream 100 bases:

>100_bases
AGGTCGCCATCCGGCGGCTGCGGGCCAAGGTGGACGACGGTTTCGAGCCGAAACTAATCCGGACGGTTCGAGGCATGGGC
TATGTGCTGGAGACAGCCGA

Downstream 100 bases:

>100_bases
CTGTTAAGCAATAAGGATCTGTTCGGGCAGTCGACAGGGAATTACTCAGCTTGGCACAGCAGTGTCAGTCTGAGCTATAG
CTCAATCTGACACACATAGA

Product: heavy metal sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNLIGKKSVTFRLTMLFASVSTTVLLMLGLLIGSLVEGHFEELDMELLGGKMELLRHALEKVRSPQELEALPGQLEDSLV
GHHGLAVRILKVDGQLLYATEGSTFPPGLLADAGKLPLRPTLWSDSENHLYRGIAALAPTGIQGMAPVVVAASTDMTHHE
HFMHSFKAALWSVVGFAALLTGFLGWVAARRGLAPLREISRSAAGITANRLNRRLAVESIPVELAEVAQTLNEMLARLEE
SFRRLSDFSSDLAHELRTPVSNLLTQTQVTLSKTRTLDEYQDVLASNAEEFERLSRTIADMLFLAKSDNDLIVPNRETVD
LAVEIASLSEFYEAWVEEKGIHLTCSGGGLVLGDRLMLRRAVGNLLSNAVRHTPEKGMITVCVADADNTCIVVSVENTGE
TIAPEHLPRLFDRFYRVDPSRQRFGEGAGLGLAITRSIASAHGGDVRVRSENGITAFEIWLPA

Sequences:

>Translated_463_residues
MNLIGKKSVTFRLTMLFASVSTTVLLMLGLLIGSLVEGHFEELDMELLGGKMELLRHALEKVRSPQELEALPGQLEDSLV
GHHGLAVRILKVDGQLLYATEGSTFPPGLLADAGKLPLRPTLWSDSENHLYRGIAALAPTGIQGMAPVVVAASTDMTHHE
HFMHSFKAALWSVVGFAALLTGFLGWVAARRGLAPLREISRSAAGITANRLNRRLAVESIPVELAEVAQTLNEMLARLEE
SFRRLSDFSSDLAHELRTPVSNLLTQTQVTLSKTRTLDEYQDVLASNAEEFERLSRTIADMLFLAKSDNDLIVPNRETVD
LAVEIASLSEFYEAWVEEKGIHLTCSGGGLVLGDRLMLRRAVGNLLSNAVRHTPEKGMITVCVADADNTCIVVSVENTGE
TIAPEHLPRLFDRFYRVDPSRQRFGEGAGLGLAITRSIASAHGGDVRVRSENGITAFEIWLPA
>Mature_463_residues
MNLIGKKSVTFRLTMLFASVSTTVLLMLGLLIGSLVEGHFEELDMELLGGKMELLRHALEKVRSPQELEALPGQLEDSLV
GHHGLAVRILKVDGQLLYATEGSTFPPGLLADAGKLPLRPTLWSDSENHLYRGIAALAPTGIQGMAPVVVAASTDMTHHE
HFMHSFKAALWSVVGFAALLTGFLGWVAARRGLAPLREISRSAAGITANRLNRRLAVESIPVELAEVAQTLNEMLARLEE
SFRRLSDFSSDLAHELRTPVSNLLTQTQVTLSKTRTLDEYQDVLASNAEEFERLSRTIADMLFLAKSDNDLIVPNRETVD
LAVEIASLSEFYEAWVEEKGIHLTCSGGGLVLGDRLMLRRAVGNLLSNAVRHTPEKGMITVCVADADNTCIVVSVENTGE
TIAPEHLPRLFDRFYRVDPSRQRFGEGAGLGLAITRSIASAHGGDVRVRSENGITAFEIWLPA

Specific function: Probable member of a two-component regulatory system pcoS/pcoR. May activate pcoR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=475, Percent_Identity=36.8421052631579, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1788279, Length=324, Percent_Identity=33.3333333333333, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1788393, Length=256, Percent_Identity=28.515625, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786600, Length=255, Percent_Identity=31.3725490196078, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790551, Length=308, Percent_Identity=26.9480519480519, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790346, Length=274, Percent_Identity=27.7372262773723, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789403, Length=464, Percent_Identity=25.4310344827586, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787894, Length=310, Percent_Identity=27.0967741935484, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=30.7377049180328, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI48994928, Length=221, Percent_Identity=28.5067873303167, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=31.0043668122271, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790861, Length=307, Percent_Identity=28.0130293159609, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=25.2100840336134, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787374, Length=286, Percent_Identity=24.4755244755245, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081816, Length=268, Percent_Identity=27.6119402985075, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789808, Length=248, Percent_Identity=28.6290322580645, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788549, Length=232, Percent_Identity=25, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87082128, Length=251, Percent_Identity=26.2948207171315, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50560; Mature: 50560

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIGKKSVTFRLTMLFASVSTTVLLMLGLLIGSLVEGHFEELDMELLGGKMELLRHALE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KVRSPQELEALPGQLEDSLVGHHGLAVRILKVDGQLLYATEGSTFPPGLLADAGKLPLRP
HHCCCHHHHHCCCHHHHHHHCCCCEEEEEEEECCEEEEEECCCCCCCCHHCCCCCCCCCC
TLWSDSENHLYRGIAALAPTGIQGMAPVVVAASTDMTHHEHFMHSFKAALWSVVGFAALL
CCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
TGFLGWVAARRGLAPLREISRSAAGITANRLNRRLAVESIPVELAEVAQTLNEMLARLEE
HHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
SFRRLSDFSSDLAHELRTPVSNLLTQTQVTLSKTRTLDEYQDVLASNAEEFERLSRTIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MLFLAKSDNDLIVPNRETVDLAVEIASLSEFYEAWVEEKGIHLTCSGGGLVLGDRLMLRR
HHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEHHHHHHHHH
AVGNLLSNAVRHTPEKGMITVCVADADNTCIVVSVENTGETIAPEHLPRLFDRFYRVDPS
HHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
RQRFGEGAGLGLAITRSIASAHGGDVRVRSENGITAFEIWLPA
HHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECC
>Mature Secondary Structure
MNLIGKKSVTFRLTMLFASVSTTVLLMLGLLIGSLVEGHFEELDMELLGGKMELLRHALE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KVRSPQELEALPGQLEDSLVGHHGLAVRILKVDGQLLYATEGSTFPPGLLADAGKLPLRP
HHCCCHHHHHCCCHHHHHHHCCCCEEEEEEEECCEEEEEECCCCCCCCHHCCCCCCCCCC
TLWSDSENHLYRGIAALAPTGIQGMAPVVVAASTDMTHHEHFMHSFKAALWSVVGFAALL
CCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
TGFLGWVAARRGLAPLREISRSAAGITANRLNRRLAVESIPVELAEVAQTLNEMLARLEE
HHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
SFRRLSDFSSDLAHELRTPVSNLLTQTQVTLSKTRTLDEYQDVLASNAEEFERLSRTIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MLFLAKSDNDLIVPNRETVDLAVEIASLSEFYEAWVEEKGIHLTCSGGGLVLGDRLMLRR
HHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEHHHHHHHHH
AVGNLLSNAVRHTPEKGMITVCVADADNTCIVVSVENTGETIAPEHLPRLFDRFYRVDPS
HHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
RQRFGEGAGLGLAITRSIASAHGGDVRVRSENGITAFEIWLPA
HHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8594334 [H]