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The map label for this gene is gspE [H]

Identifier: 71906605

GI number: 71906605

Start: 1049883

End: 1052123

Strand: Reverse

Name: gspE [H]

Synonym: Daro_0966

Alternate gene names: 71906605

Gene position: 1052123-1049883 (Counterclockwise)

Preceding gene: 71906606

Following gene: 71906604

Centisome position: 23.37

GC content: 60.64

Gene sequence:

>2241_bases
ATGACTGACTATTCGGCACTCTTCACCGGCCAGACCAGCACGCCGCAAGCGCCGGCCAGTCACCTGACGCCGCCGAAATA
CCGCCTGCTGATCGTCGATGACGAAGTCGGCATCGTCAAGGCGCTGACGCGCGTATTCCGTCAGGAAAACTATGAAGTCG
TGACTGCCGGCAGCGGCAACGAAGGTCTGCAGCGCATGGCAGAGGGCAATTTCCAGCTGATCATCAGCGACTTCATGATG
CCTGGCATGAACGGCGCCCAGTTCCTGCGCGAGGTCAAGCAACGTTCACCGGAAACGATCCGCATCATGCTGACCGGCCA
TGCCAATACCGATGCCGTGATGGGCGCCATCAACGAAGGTGCGGTATACAAATTCATCCTCAAGCCGTGGAACGATGACG
ATCTGCGTGTCACCGTCGCACTGGCTTTGGAGCAGTTCGACCTGATCACCCGCAACCGGGTTCTGAAGACCGAAAACGAG
AAGAAATCCAAGGAAATCTCGGCGCTGAATCGCCTGGCGGTGACCCACCGCAGTCGACTGGGCATCATGCTGCACAAGAA
AAACCTGCTCAACGCGGCCCAGTTGCAGGAACTGACCTTGCTGCAGGAGCGACGCAAGGAGCCGCTGATCGCCCTGCTCC
TCGAAAAGGACTGGATCAGCGAGCGGCAGATTCGCACCCTGCTCAAGTCGGAAATGCTGATCGACGAGGTCCAGCTACCA
GAATTCCGCATCGATGCAGTGCTGGCCGAGCTGATCCCGCACAGTTTCTGCCAGCGCCAACTGGTCGTCCCGCTCAAGCT
GGAAGGCCGGCGCCTGCTGCTGGCGATGGCAGACCCGCTCGACGAGGGCCTGATCGACGAAGTTCGTTTCACGGCCGGTC
TCGACCTGAAAATTGTCACGGCCGACACCGAGGCAATCCGCAACAAAATCAATGAGCTGTACGGCGAAGGCGGCGTCGAT
TTCAAGGAACTGGAGACCCTGGTCAGCAGCCCGGACCCTTACGAGGGGATCGAGATCGTCATCGAGGAGGAGGACACCGC
CCAGCTCGAAGACCTGCTCCGCGAAACCGAGGCCCCGCCAGCCATCCGTCTGGCCAACGCAATCATTCTCGAAGCCATCC
GGCTCGGCGCTTCCGACATCCACATCCAACCCCGGATCAAGTCCGTTGTCGTCCGCTACCGGATCGACGGCGTGCTGACC
GACAAGATTCAGATCCCGCACCACCTGCACCAGTCGCTGGTGTCGCGCCTGAAGATCATGTCCGAACTGGACATTTCCGA
ACGCCGGCGACCACAGGATGGCCGGATCACCGTCAAGACGCCGATGCGCATGGTGGATCTGCGCATTTCCACCCTGCCGA
CGATCAACGGCGAGAAGGTGGTCATGCGCATTCTCGACCGCAATTCAACGGTGCATACGCTGGACCGCCTTGGCTTCTCG
CCCGTCGACCTGCCGCGCGTCGCCGACATGGTGGCCCGGCCGCAGGGCATCATCCTGGCGACCGGCCCGACCGGCAGCGG
CAAGACAACCACCTTGTATTCGCTGCTCCAACACGATGCAACACCTGACAAGAACTACGTCACCATCGAAGATCCGGTCG
AGTACTACCTCGACATGGCCGGGCAGGTCCTGGTCAAGGAAAAGATCGGTCTGACTTTCCCATCCATTTTGCGTGCCTTG
TTGCGCCAGGATCCGGATGTTGTGCTGCTCGGCGAAATCCGTGACTTCGAAACCGCCGAGGTGGCTTTTCATGCCGCCCT
GACCGGCCACCTCGTTTATTCGACCCTGCACACCAATTCGGCGATCGCGACGATTGCTCGCCTGTTCGACCTTGGCCTCA
AACCCTATATTGTCGCCACGGCGCTGGAAGGCATCATCGCTCAGCGACTGGTCCGCCAGATCTGCCCGCATTGCCGTACC
GAGGTCGATGCCGAGCCGGCACTGCGGGCAAGACTGGGCGGTCACTTTGCCCTGCTTGAACGAAGCTATCGCGGCGCCGG
CTGCAACCACTGTCATGGCTCGGGTTACAAAGGGCGGGTCGGTCTGTATGAAGTACTGGCGCTTGATGACGAATTACGCG
ACCGGATCGGCAGCGGTGCCAGCGTGCTGGAAATTCGTCGCCTGGTCCGCCAGAAAGGCTTGCGGGGCATTGGTTGCCAC
GCCTTGGAGCGCGTTCAGTCCGGCGCTACCAGCCTGGAGGAAATTCTGCGTGTTCTCGGCCCACAGGCCGGAGAGGACTA
A

Upstream 100 bases:

>100_bases
GCACTGCTCGCAACATCGTCCTCGCCCATAGCGGCCATCTCACGATTGCCAGCGAACCGGGGCAAGGCACCACGATCAAC
CTCAGTTTTCCGACACCACC

Downstream 100 bases:

>100_bases
AAATGGACGCATTTGTCTGGAACGAGCGCTACATGACAGGAGAAGCGCAGGTTGATGAGGAGCATCAGGAACTGGTGCGT
ATCATCAACTGGGTCATCGA

Product: type II secretion system protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 746; Mature: 745

Protein sequence:

>746_residues
MTDYSALFTGQTSTPQAPASHLTPPKYRLLIVDDEVGIVKALTRVFRQENYEVVTAGSGNEGLQRMAEGNFQLIISDFMM
PGMNGAQFLREVKQRSPETIRIMLTGHANTDAVMGAINEGAVYKFILKPWNDDDLRVTVALALEQFDLITRNRVLKTENE
KKSKEISALNRLAVTHRSRLGIMLHKKNLLNAAQLQELTLLQERRKEPLIALLLEKDWISERQIRTLLKSEMLIDEVQLP
EFRIDAVLAELIPHSFCQRQLVVPLKLEGRRLLLAMADPLDEGLIDEVRFTAGLDLKIVTADTEAIRNKINELYGEGGVD
FKELETLVSSPDPYEGIEIVIEEEDTAQLEDLLRETEAPPAIRLANAIILEAIRLGASDIHIQPRIKSVVVRYRIDGVLT
DKIQIPHHLHQSLVSRLKIMSELDISERRRPQDGRITVKTPMRMVDLRISTLPTINGEKVVMRILDRNSTVHTLDRLGFS
PVDLPRVADMVARPQGIILATGPTGSGKTTTLYSLLQHDATPDKNYVTIEDPVEYYLDMAGQVLVKEKIGLTFPSILRAL
LRQDPDVVLLGEIRDFETAEVAFHAALTGHLVYSTLHTNSAIATIARLFDLGLKPYIVATALEGIIAQRLVRQICPHCRT
EVDAEPALRARLGGHFALLERSYRGAGCNHCHGSGYKGRVGLYEVLALDDELRDRIGSGASVLEIRRLVRQKGLRGIGCH
ALERVQSGATSLEEILRVLGPQAGED

Sequences:

>Translated_746_residues
MTDYSALFTGQTSTPQAPASHLTPPKYRLLIVDDEVGIVKALTRVFRQENYEVVTAGSGNEGLQRMAEGNFQLIISDFMM
PGMNGAQFLREVKQRSPETIRIMLTGHANTDAVMGAINEGAVYKFILKPWNDDDLRVTVALALEQFDLITRNRVLKTENE
KKSKEISALNRLAVTHRSRLGIMLHKKNLLNAAQLQELTLLQERRKEPLIALLLEKDWISERQIRTLLKSEMLIDEVQLP
EFRIDAVLAELIPHSFCQRQLVVPLKLEGRRLLLAMADPLDEGLIDEVRFTAGLDLKIVTADTEAIRNKINELYGEGGVD
FKELETLVSSPDPYEGIEIVIEEEDTAQLEDLLRETEAPPAIRLANAIILEAIRLGASDIHIQPRIKSVVVRYRIDGVLT
DKIQIPHHLHQSLVSRLKIMSELDISERRRPQDGRITVKTPMRMVDLRISTLPTINGEKVVMRILDRNSTVHTLDRLGFS
PVDLPRVADMVARPQGIILATGPTGSGKTTTLYSLLQHDATPDKNYVTIEDPVEYYLDMAGQVLVKEKIGLTFPSILRAL
LRQDPDVVLLGEIRDFETAEVAFHAALTGHLVYSTLHTNSAIATIARLFDLGLKPYIVATALEGIIAQRLVRQICPHCRT
EVDAEPALRARLGGHFALLERSYRGAGCNHCHGSGYKGRVGLYEVLALDDELRDRIGSGASVLEIRRLVRQKGLRGIGCH
ALERVQSGATSLEEILRVLGPQAGED
>Mature_745_residues
TDYSALFTGQTSTPQAPASHLTPPKYRLLIVDDEVGIVKALTRVFRQENYEVVTAGSGNEGLQRMAEGNFQLIISDFMMP
GMNGAQFLREVKQRSPETIRIMLTGHANTDAVMGAINEGAVYKFILKPWNDDDLRVTVALALEQFDLITRNRVLKTENEK
KSKEISALNRLAVTHRSRLGIMLHKKNLLNAAQLQELTLLQERRKEPLIALLLEKDWISERQIRTLLKSEMLIDEVQLPE
FRIDAVLAELIPHSFCQRQLVVPLKLEGRRLLLAMADPLDEGLIDEVRFTAGLDLKIVTADTEAIRNKINELYGEGGVDF
KELETLVSSPDPYEGIEIVIEEEDTAQLEDLLRETEAPPAIRLANAIILEAIRLGASDIHIQPRIKSVVVRYRIDGVLTD
KIQIPHHLHQSLVSRLKIMSELDISERRRPQDGRITVKTPMRMVDLRISTLPTINGEKVVMRILDRNSTVHTLDRLGFSP
VDLPRVADMVARPQGIILATGPTGSGKTTTLYSLLQHDATPDKNYVTIEDPVEYYLDMAGQVLVKEKIGLTFPSILRALL
RQDPDVVLLGEIRDFETAEVAFHAALTGHLVYSTLHTNSAIATIARLFDLGLKPYIVATALEGIIAQRLVRQICPHCRTE
VDAEPALRARLGGHFALLERSYRGAGCNHCHGSGYKGRVGLYEVLALDDELRDRIGSGASVLEIRRLVRQKGLRGIGCHA
LERVQSGATSLEEILRVLGPQAGED

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=407, Percent_Identity=45.945945945946, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1786296, Length=389, Percent_Identity=43.7017994858612, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI87082188, Length=168, Percent_Identity=43.452380952381, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788905, Length=117, Percent_Identity=38.4615384615385, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790299, Length=118, Percent_Identity=30.5084745762712, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1788550, Length=117, Percent_Identity=29.9145299145299, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1790437, Length=122, Percent_Identity=28.6885245901639, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 83250; Mature: 83118

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYSALFTGQTSTPQAPASHLTPPKYRLLIVDDEVGIVKALTRVFRQENYEVVTAGSGN
CCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCC
EGLQRMAEGNFQLIISDFMMPGMNGAQFLREVKQRSPETIRIMLTGHANTDAVMGAINEG
HHHHHHHCCCCEEEEEHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCC
AVYKFILKPWNDDDLRVTVALALEQFDLITRNRVLKTENEKKSKEISALNRLAVTHRSRL
CEEEEEECCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GIMLHKKNLLNAAQLQELTLLQERRKEPLIALLLEKDWISERQIRTLLKSEMLIDEVQLP
EEEEECCHHCCHHHHHHHHHHHHHCCCCEEEEEECHHCCCHHHHHHHHHHHHHHHHHCCC
EFRIDAVLAELIPHSFCQRQLVVPLKLEGRRLLLAMADPLDEGLIDEVRFTAGLDLKIVT
CHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEE
ADTEAIRNKINELYGEGGVDFKELETLVSSPDPYEGIEIVIEEEDTAQLEDLLRETEAPP
CCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCC
AIRLANAIILEAIRLGASDIHIQPRIKSVVVRYRIDGVLTDKIQIPHHLHQSLVSRLKIM
HHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHEECCEEECCCCCCHHHHHHHHHHHHHH
SELDISERRRPQDGRITVKTPMRMVDLRISTLPTINGEKVVMRILDRNSTVHTLDRLGFS
HHCCHHHHCCCCCCEEEEECCCEEEEEEHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCC
PVDLPRVADMVARPQGIILATGPTGSGKTTTLYSLLQHDATPDKNYVTIEDPVEYYLDMA
CCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHC
GQVLVKEKIGLTFPSILRALLRQDPDVVLLGEIRDFETAEVAFHAALTGHLVYSTLHTNS
CCEEEHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCH
AIATIARLFDLGLKPYIVATALEGIIAQRLVRQICPHCRTEVDAEPALRARLGGHFALLE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHEEHH
RSYRGAGCNHCHGSGYKGRVGLYEVLALDDELRDRIGSGASVLEIRRLVRQKGLRGIGCH
HHCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHH
ALERVQSGATSLEEILRVLGPQAGED
HHHHHHHCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDYSALFTGQTSTPQAPASHLTPPKYRLLIVDDEVGIVKALTRVFRQENYEVVTAGSGN
CCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCC
EGLQRMAEGNFQLIISDFMMPGMNGAQFLREVKQRSPETIRIMLTGHANTDAVMGAINEG
HHHHHHHCCCCEEEEEHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCC
AVYKFILKPWNDDDLRVTVALALEQFDLITRNRVLKTENEKKSKEISALNRLAVTHRSRL
CEEEEEECCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GIMLHKKNLLNAAQLQELTLLQERRKEPLIALLLEKDWISERQIRTLLKSEMLIDEVQLP
EEEEECCHHCCHHHHHHHHHHHHHCCCCEEEEEECHHCCCHHHHHHHHHHHHHHHHHCCC
EFRIDAVLAELIPHSFCQRQLVVPLKLEGRRLLLAMADPLDEGLIDEVRFTAGLDLKIVT
CHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEE
ADTEAIRNKINELYGEGGVDFKELETLVSSPDPYEGIEIVIEEEDTAQLEDLLRETEAPP
CCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCC
AIRLANAIILEAIRLGASDIHIQPRIKSVVVRYRIDGVLTDKIQIPHHLHQSLVSRLKIM
HHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHEECCEEECCCCCCHHHHHHHHHHHHHH
SELDISERRRPQDGRITVKTPMRMVDLRISTLPTINGEKVVMRILDRNSTVHTLDRLGFS
HHCCHHHHCCCCCCEEEEECCCEEEEEEHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCC
PVDLPRVADMVARPQGIILATGPTGSGKTTTLYSLLQHDATPDKNYVTIEDPVEYYLDMA
CCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHC
GQVLVKEKIGLTFPSILRALLRQDPDVVLLGEIRDFETAEVAFHAALTGHLVYSTLHTNS
CCEEEHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCH
AIATIARLFDLGLKPYIVATALEGIIAQRLVRQICPHCRTEVDAEPALRARLGGHFALLE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHEEHH
RSYRGAGCNHCHGSGYKGRVGLYEVLALDDELRDRIGSGASVLEIRRLVRQKGLRGIGCH
HHCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHH
ALERVQSGATSLEEILRVLGPQAGED
HHHHHHHCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]