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The map label for this gene is zraS [H]

Identifier: 71906604

GI number: 71906604

Start: 1047835

End: 1049880

Strand: Reverse

Name: zraS [H]

Synonym: Daro_0965

Alternate gene names: 71906604

Gene position: 1049880-1047835 (Counterclockwise)

Preceding gene: 71906605

Following gene: 71906603

Centisome position: 23.32

GC content: 57.67

Gene sequence:

>2046_bases
ATGGACGCATTTGTCTGGAACGAGCGCTACATGACAGGAGAAGCGCAGGTTGATGAGGAGCATCAGGAACTGGTGCGTAT
CATCAACTGGGTCATCGAACACCAATCCGTGCCGGCGCAGGATGACGATATCCAGCGGGTATTGGCCAGCTTGGCCAACT
ATGCCGTGATGCACTTCGGACACGAGGAGGAGCTGATGGAGCAGATGCATTGTGACCCCCGCTTCATTCAGGCGCACGTC
GCGGTGCATCAGGAATTTGCCCGCCAAGTCGGAAAAATGCTGGAGTTCCAGGGCGGCAAAGCCGATGTTGAATATCTGCT
GCGTTTCCTCAGTAGCTGGCTGGCTCATCACATCCTCGGTATTGATCAGTCGATGGCCCGCCAGATCGCAAAGATCCGTG
CCGGGGCCGCGCCGGAAGCGGCTTTCGAGGCCGAGAAAAGAATCCCTCATGACCCGTCGACCGCCAGCCTGCTCGATGCG
ATGAACGCCTTGTTCCGCATGGTCGCCAGTCGCAACGAGGCATTGGAGAAGCTCAATGCCAGCCTGGGGGCGCAAGTCGC
AGCACAAACAGCTGATCTGATGCAATCCAATCAACAGTTGCTGGTCGAGCAAGGGCGACTGCAATTGGCCATGAAGCAGA
TTGAAATCACCCAGAAAAAGCTGCTCGAGTCCGAACACCGGCGGTCCGAGGCCGCCAAGCGCGGCATGGAGCAATTGCTT
GCCCAGATCATCGATGGCGATCCGGTGCCGACCTTCGTGATCGATGCCCAGCATCGGATCACCCATTGGAACAAGGCTTG
CGCCGTGGTCAGCGGCCTGTCCAGCGCAGAGATGGTGGGGACCCAGTTACACTGGAAGGCCTTCTACAAGGAATACCGCC
CGGTGATGGCGGACCTGATCGTCAATGGCAGCCTGGAAGCGCAGTACGAGGAATATTACAAGGATCGCTTCAGGCGCTCT
TCATTGATTCAGGATGCGTTTGAAGGCGAGGCCTTTTTTGAAGGCCTGGGGCCGAATGGCTTGTGGCTGGCTTTCACGGC
AGCCCCGCTACGCAACGCCAATGGTGAGTTGATCGGTGCAATCGAGACCTTGCAGGACGTCACCGAACGGCACCGCGCCC
AGGAAAGCTTGCTGCAACACCAGAACCAGCTGGAAAGCCTGGTCGTTCAACGCACGACCCAGTTGGCCACCGCCAATACC
GAACTGGAAAACGAGCGGCGCGAGCTCGAAGTCCTGCTTTCAAAAATTGAAGAAGCCCAGCAGCAATTGCTGCAGTCGGA
AAAAATGGCGGCAATTGGCCAACTCGCGGCTGGCGTCGCCCACGAAATCAACAACCCTGTCGGCTTCGTCAACTCCAACC
TGGGAACGCTGAAGACCTATATCAACAACCTGCTCGGCGTCGTCGACGCCTATGAAGCGGTCGCGGCAGGCGGCGACCCT
GCCCAAATTGAGGCTGCCCGGCGAGCGGCCGACATCGACTTTCTGCGTGAAGACCTGCCTTCCCTGCTCATCGAATCCCA
GGAGGGCTTGGGACGCGTGACCAAGATTGTCCAGGACCTCAAGGACTTTTCCCGTGTCGATCAGGCCGAATATCAACTGG
CCAATCTCAACGCTGCCCTGGAAAGCACCCTGAACGTTGTCTGGAACGAGTTGAAATACAAAGCCGACGTGGTCCGCGAT
CTGGGCGATATCCCTGAAGTCGAGTGTGTACCGGCCCAGATCAACCAGGTTTTCATGAACTTGCTGGTCAATGCCGCACA
GGCCATCGAGACACACGGCAGGATCACGGTCCGTTCCGGCCAGGAGAACGCCCATGTCTGGTTCGAGATTTCCGACACCG
GTAAAGGCATGACGGCGGAAGTATGCCACCGCGTATTCGACCCCTTCTTCACCACCAAGCCGGTCGGCCAGGGTACCGGC
CTCGGGCTGTCGATCTCCTACGACATCATTGTCAAAAAACATCGAGGACGTTTCGATGTCGCCAGCACCCCGGGCCAAGG
AACAACTTTCAAGTTCTGGCTACCGATTCAGGGCGTGGAAAAATGA

Upstream 100 bases:

>100_bases
GGGGCATTGGTTGCCACGCCTTGGAGCGCGTTCAGTCCGGCGCTACCAGCCTGGAGGAAATTCTGCGTGTTCTCGGCCCA
CAGGCCGGAGAGGACTAAAA

Downstream 100 bases:

>100_bases
CCTCCCCTTAGCGGGCAGGGGCAGCACCAATGTGCTCATTGCCCCCGCAAACGGGACCAACGACAAGACACAAGACGTCG
CTCAATAACGGCCTCCGAAC

Product: PAS:hemerythrin HHE cation binding region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MDAFVWNERYMTGEAQVDEEHQELVRIINWVIEHQSVPAQDDDIQRVLASLANYAVMHFGHEEELMEQMHCDPRFIQAHV
AVHQEFARQVGKMLEFQGGKADVEYLLRFLSSWLAHHILGIDQSMARQIAKIRAGAAPEAAFEAEKRIPHDPSTASLLDA
MNALFRMVASRNEALEKLNASLGAQVAAQTADLMQSNQQLLVEQGRLQLAMKQIEITQKKLLESEHRRSEAAKRGMEQLL
AQIIDGDPVPTFVIDAQHRITHWNKACAVVSGLSSAEMVGTQLHWKAFYKEYRPVMADLIVNGSLEAQYEEYYKDRFRRS
SLIQDAFEGEAFFEGLGPNGLWLAFTAAPLRNANGELIGAIETLQDVTERHRAQESLLQHQNQLESLVVQRTTQLATANT
ELENERRELEVLLSKIEEAQQQLLQSEKMAAIGQLAAGVAHEINNPVGFVNSNLGTLKTYINNLLGVVDAYEAVAAGGDP
AQIEAARRAADIDFLREDLPSLLIESQEGLGRVTKIVQDLKDFSRVDQAEYQLANLNAALESTLNVVWNELKYKADVVRD
LGDIPEVECVPAQINQVFMNLLVNAAQAIETHGRITVRSGQENAHVWFEISDTGKGMTAEVCHRVFDPFFTTKPVGQGTG
LGLSISYDIIVKKHRGRFDVASTPGQGTTFKFWLPIQGVEK

Sequences:

>Translated_681_residues
MDAFVWNERYMTGEAQVDEEHQELVRIINWVIEHQSVPAQDDDIQRVLASLANYAVMHFGHEEELMEQMHCDPRFIQAHV
AVHQEFARQVGKMLEFQGGKADVEYLLRFLSSWLAHHILGIDQSMARQIAKIRAGAAPEAAFEAEKRIPHDPSTASLLDA
MNALFRMVASRNEALEKLNASLGAQVAAQTADLMQSNQQLLVEQGRLQLAMKQIEITQKKLLESEHRRSEAAKRGMEQLL
AQIIDGDPVPTFVIDAQHRITHWNKACAVVSGLSSAEMVGTQLHWKAFYKEYRPVMADLIVNGSLEAQYEEYYKDRFRRS
SLIQDAFEGEAFFEGLGPNGLWLAFTAAPLRNANGELIGAIETLQDVTERHRAQESLLQHQNQLESLVVQRTTQLATANT
ELENERRELEVLLSKIEEAQQQLLQSEKMAAIGQLAAGVAHEINNPVGFVNSNLGTLKTYINNLLGVVDAYEAVAAGGDP
AQIEAARRAADIDFLREDLPSLLIESQEGLGRVTKIVQDLKDFSRVDQAEYQLANLNAALESTLNVVWNELKYKADVVRD
LGDIPEVECVPAQINQVFMNLLVNAAQAIETHGRITVRSGQENAHVWFEISDTGKGMTAEVCHRVFDPFFTTKPVGQGTG
LGLSISYDIIVKKHRGRFDVASTPGQGTTFKFWLPIQGVEK
>Mature_681_residues
MDAFVWNERYMTGEAQVDEEHQELVRIINWVIEHQSVPAQDDDIQRVLASLANYAVMHFGHEEELMEQMHCDPRFIQAHV
AVHQEFARQVGKMLEFQGGKADVEYLLRFLSSWLAHHILGIDQSMARQIAKIRAGAAPEAAFEAEKRIPHDPSTASLLDA
MNALFRMVASRNEALEKLNASLGAQVAAQTADLMQSNQQLLVEQGRLQLAMKQIEITQKKLLESEHRRSEAAKRGMEQLL
AQIIDGDPVPTFVIDAQHRITHWNKACAVVSGLSSAEMVGTQLHWKAFYKEYRPVMADLIVNGSLEAQYEEYYKDRFRRS
SLIQDAFEGEAFFEGLGPNGLWLAFTAAPLRNANGELIGAIETLQDVTERHRAQESLLQHQNQLESLVVQRTTQLATANT
ELENERRELEVLLSKIEEAQQQLLQSEKMAAIGQLAAGVAHEINNPVGFVNSNLGTLKTYINNLLGVVDAYEAVAAGGDP
AQIEAARRAADIDFLREDLPSLLIESQEGLGRVTKIVQDLKDFSRVDQAEYQLANLNAALESTLNVVWNELKYKADVVRD
LGDIPEVECVPAQINQVFMNLLVNAAQAIETHGRITVRSGQENAHVWFEISDTGKGMTAEVCHRVFDPFFTTKPVGQGTG
LGLSISYDIIVKKHRGRFDVASTPGQGTTFKFWLPIQGVEK

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=265, Percent_Identity=32.0754716981132, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788549, Length=430, Percent_Identity=26.9767441860465, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI145693157, Length=369, Percent_Identity=25.2032520325203, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789808, Length=166, Percent_Identity=32.5301204819277, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87081816, Length=316, Percent_Identity=26.2658227848101, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 76078; Mature: 76078

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAFVWNERYMTGEAQVDEEHQELVRIINWVIEHQSVPAQDDDIQRVLASLANYAVMHFG
CCCEEECCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
HEEELMEQMHCDPRFIQAHVAVHQEFARQVGKMLEFQGGKADVEYLLRFLSSWLAHHILG
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
IDQSMARQIAKIRAGAAPEAAFEAEKRIPHDPSTASLLDAMNALFRMVASRNEALEKLNA
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGAQVAAQTADLMQSNQQLLVEQGRLQLAMKQIEITQKKLLESEHRRSEAAKRGMEQLL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQIIDGDPVPTFVIDAQHRITHWNKACAVVSGLSSAEMVGTQLHWKAFYKEYRPVMADLI
HHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VNGSLEAQYEEYYKDRFRRSSLIQDAFEGEAFFEGLGPNGLWLAFTAAPLRNANGELIGA
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCCCEEHH
IETLQDVTERHRAQESLLQHQNQLESLVVQRTTQLATANTELENERRELEVLLSKIEEAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QQLLQSEKMAAIGQLAAGVAHEINNPVGFVNSNLGTLKTYINNLLGVVDAYEAVAAGGDP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
AQIEAARRAADIDFLREDLPSLLIESQEGLGRVTKIVQDLKDFSRVDQAEYQLANLNAAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESTLNVVWNELKYKADVVRDLGDIPEVECVPAQINQVFMNLLVNAAQAIETHGRITVRSG
HHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
QENAHVWFEISDTGKGMTAEVCHRVFDPFFTTKPVGQGTGLGLSISYDIIVKKHRGRFDV
CCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCC
ASTPGQGTTFKFWLPIQGVEK
CCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MDAFVWNERYMTGEAQVDEEHQELVRIINWVIEHQSVPAQDDDIQRVLASLANYAVMHFG
CCCEEECCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
HEEELMEQMHCDPRFIQAHVAVHQEFARQVGKMLEFQGGKADVEYLLRFLSSWLAHHILG
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
IDQSMARQIAKIRAGAAPEAAFEAEKRIPHDPSTASLLDAMNALFRMVASRNEALEKLNA
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGAQVAAQTADLMQSNQQLLVEQGRLQLAMKQIEITQKKLLESEHRRSEAAKRGMEQLL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQIIDGDPVPTFVIDAQHRITHWNKACAVVSGLSSAEMVGTQLHWKAFYKEYRPVMADLI
HHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VNGSLEAQYEEYYKDRFRRSSLIQDAFEGEAFFEGLGPNGLWLAFTAAPLRNANGELIGA
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCCCEEHH
IETLQDVTERHRAQESLLQHQNQLESLVVQRTTQLATANTELENERRELEVLLSKIEEAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QQLLQSEKMAAIGQLAAGVAHEINNPVGFVNSNLGTLKTYINNLLGVVDAYEAVAAGGDP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
AQIEAARRAADIDFLREDLPSLLIESQEGLGRVTKIVQDLKDFSRVDQAEYQLANLNAAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESTLNVVWNELKYKADVVRDLGDIPEVECVPAQINQVFMNLLVNAAQAIETHGRITVRSG
HHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
QENAHVWFEISDTGKGMTAEVCHRVFDPFFTTKPVGQGTGLGLSISYDIIVKKHRGRFDV
CCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCC
ASTPGQGTTFKFWLPIQGVEK
CCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]