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The map label for this gene is fliN [H]

Identifier: 71906404

GI number: 71906404

Start: 833234

End: 833677

Strand: Reverse

Name: fliN [H]

Synonym: Daro_0765

Alternate gene names: 71906404

Gene position: 833677-833234 (Counterclockwise)

Preceding gene: 71906405

Following gene: 71906403

Centisome position: 18.52

GC content: 56.08

Gene sequence:

>444_bases
ATGGCTGACGAAATGGAAAACATCGAGAGCACCCCCGTGGAAGATGACGGCCAGATCAGCGAGGACGATTGGGCTGCAGC
GATGTCCGAACAGGCCATTAGCGATGCCCCTGCCGCGATGGCTGCACAACCGGCCGACATCTTTCCGTCGTTTGGCGGCA
ACGCAGGTAATGCCGGCATGATGAACGAGCTCGACATGATTCTGGACATTCCAGTCCAAATTACCGTCGAACTCGGCCGG
ACCAAGATCACCATCAAGAATCTGCTCCAGCTTGCCCACGGCTCAGTTGTTGAACTGGATGCAATGGCCGGCGAGCCGAT
GGATGTCATGGTCAATGGCACGCTGATCGCCCAGGGCGAAGTCGTGGTGGTTAACGACAAATTCGGCATCCGCCTGACCG
ATATCATTACCCCTTCCGAACGGATGCGAAAACTCAATCGCTAA

Upstream 100 bases:

>100_bases
ACCTACGGGCAGCAAAGCGGCCAATATGCGCTGAAGGTCGATCGATTCATTGCCTCCTCGCCCGAAACACCGCATGCCGA
ACCGGCAACAGGAGAAAAAA

Downstream 100 bases:

>100_bases
ACGCCCAGCCTAGTTTCGATTAAGATAGCGGACTAATATCTCCGCATACGGGCTTGGCTTTGTTACGTTTTCTTCTCTTG
TTCACTCCTTTCGCTGCGCA

Product: Type III secretion system outer membrane O protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MADEMENIESTPVEDDGQISEDDWAAAMSEQAISDAPAAMAAQPADIFPSFGGNAGNAGMMNELDMILDIPVQITVELGR
TKITIKNLLQLAHGSVVELDAMAGEPMDVMVNGTLIAQGEVVVVNDKFGIRLTDIITPSERMRKLNR

Sequences:

>Translated_147_residues
MADEMENIESTPVEDDGQISEDDWAAAMSEQAISDAPAAMAAQPADIFPSFGGNAGNAGMMNELDMILDIPVQITVELGR
TKITIKNLLQLAHGSVVELDAMAGEPMDVMVNGTLIAQGEVVVVNDKFGIRLTDIITPSERMRKLNR
>Mature_146_residues
ADEMENIESTPVEDDGQISEDDWAAAMSEQAISDAPAAMAAQPADIFPSFGGNAGNAGMMNELDMILDIPVQITVELGRT
KITIKNLLQLAHGSVVELDAMAGEPMDVMVNGTLIAQGEVVVVNDKFGIRLTDIITPSERMRKLNR

Specific function: FliN is one of three proteins (FliG, FliN, FliM) that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=136, Percent_Identity=54.4117647058823, Blast_Score=153, Evalue=4e-39,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 15773; Mature: 15642

Theoretical pI: Translated: 3.84; Mature: 3.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
7.5 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEMENIESTPVEDDGQISEDDWAAAMSEQAISDAPAAMAAQPADIFPSFGGNAGNAGM
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCC
MNELDMILDIPVQITVELGRTKITIKNLLQLAHGSVVELDAMAGEPMDVMVNGTLIAQGE
CCCCCEEEECCEEEEEEECCEEEEHHHHHHHHCCCEEEEECCCCCCEEEEEECEEEECCC
VVVVNDKFGIRLTDIITPSERMRKLNR
EEEEECCCCEEEEEEECCHHHHHHCCC
>Mature Secondary Structure 
ADEMENIESTPVEDDGQISEDDWAAAMSEQAISDAPAAMAAQPADIFPSFGGNAGNAGM
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCC
MNELDMILDIPVQITVELGRTKITIKNLLQLAHGSVVELDAMAGEPMDVMVNGTLIAQGE
CCCCCEEEECCEEEEEEECCEEEEHHHHHHHHCCCEEEEECCCCCCEEEEEECEEEECCC
VVVVNDKFGIRLTDIITPSERMRKLNR
EEEEECCCCEEEEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2651416; 9097040; 9278503 [H]