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The map label for this gene is paaX [H]

Identifier: 71906028

GI number: 71906028

Start: 417912

End: 418970

Strand: Reverse

Name: paaX [H]

Synonym: Daro_0386

Alternate gene names: 71906028

Gene position: 418970-417912 (Counterclockwise)

Preceding gene: 71906029

Following gene: 71906026

Centisome position: 9.31

GC content: 64.68

Gene sequence:

>1059_bases
ATGCTCAACACTGGCATACAAAACGATACTCGGCATCAGGTACAATCGAAGTCTTCAACGGGTCGCCATCGGTCCGAGCC
ATTTCCTCAACGCCCTTCGCCAGCCTATCTCGTGAGCACCGCCATCCAATCCCGCCTGAATGAATTCCGGCAACAGCGCC
GTGTCCAGGCTGGCTCGCTGATCATCACCGTCTTTGGCGACGCGATCCTGCCGCGCGGCGGACGCATCTGGCTAGGCAGC
CTGATCCGCCTGCTCGAACCACTCGAACTCAACGAACGGCTGATCCGCACCTCCGTCTTCCGTCTGGTCAAGGAGGAATG
GCTGCGCACCGAAACCATCGGCCGGCGTGCCGACTACGTGCTGACGCCATCGGGCCGTCGGCGTTTCGAGGAAGCTTCAC
GCCACATCTACGCCTCGGATGCGCCACTCTGGGATCGCCGCTGGCGCCTGATCCTGGTCGTCGGCGATCTGGACCCCAAG
CTGCGTGAGCAGGTCCGGCGCGCCTTGTTCTGGCAGGGGTTCGGCGCCTTGGGGGCCGATTGCTTCGTGCACCCTAGCGC
CGAGTTGTCCAGCGTGCTCGACACGCTGATTACCGAAGGCCTGTCATCGGCCATCGGCGCGCTGATGCCCTTGTTCGCGG
CCGATTCGCGTTCGGCCCAGTCGGCCAGCGACGCCGACCTCGTGCACCGCGCCTGGGATCTCGGGCATCTGGCCGAGGCC
TACAGCGCCTTCGTCGCCACCTATCAGCCCATTCTCGACGAACTCCGGCGCGACCATCTGGCCGGGGTCAGCGAGCAGGA
TGCCTTCCTGCTGCGCATCCTGCTCATCCACGATTACCGGCGCCTGCTGCTGCGCGATCCGGAATTGCCGGAAGTCCTGC
TGCCGGCCAACTGGCCAGGTCAGCAGTCGCGACTGTTGTGCAAGGAACTGTACAAGCGGCTGGAACCCCTCGCCAGCCGC
CACCTCGACCAGCAGTTGTGCCTGGCCGATGGACGCGTGCCGGAAGAGGACCTGTCGCTCCCCGAGCGCTTCCCGCAGAA
CGATCCGCTATCGGCCTGA

Upstream 100 bases:

>100_bases
TCCGACAGAGATAAACAGGTACAAAAATACAAAAATATTTGTATTTTCCGTATCAGTAAGTCCTGAAAAAATTCAACTTG
CCTGATGATTCGCGGGTTTG

Downstream 100 bases:

>100_bases
TCAGGCCGAGTCGTCAGTCCCGCCGATTCTGCCCTGTTCGGCCCACCCCGCATCCCCATTCGCTTTATCGCGCGACCGGG
GCGCGGAGCCTGCCTCGGCA

Product: phenylacetic acid degradation operon negative regulatory protein PaaX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MLNTGIQNDTRHQVQSKSSTGRHRSEPFPQRPSPAYLVSTAIQSRLNEFRQQRRVQAGSLIITVFGDAILPRGGRIWLGS
LIRLLEPLELNERLIRTSVFRLVKEEWLRTETIGRRADYVLTPSGRRRFEEASRHIYASDAPLWDRRWRLILVVGDLDPK
LREQVRRALFWQGFGALGADCFVHPSAELSSVLDTLITEGLSSAIGALMPLFAADSRSAQSASDADLVHRAWDLGHLAEA
YSAFVATYQPILDELRRDHLAGVSEQDAFLLRILLIHDYRRLLLRDPELPEVLLPANWPGQQSRLLCKELYKRLEPLASR
HLDQQLCLADGRVPEEDLSLPERFPQNDPLSA

Sequences:

>Translated_352_residues
MLNTGIQNDTRHQVQSKSSTGRHRSEPFPQRPSPAYLVSTAIQSRLNEFRQQRRVQAGSLIITVFGDAILPRGGRIWLGS
LIRLLEPLELNERLIRTSVFRLVKEEWLRTETIGRRADYVLTPSGRRRFEEASRHIYASDAPLWDRRWRLILVVGDLDPK
LREQVRRALFWQGFGALGADCFVHPSAELSSVLDTLITEGLSSAIGALMPLFAADSRSAQSASDADLVHRAWDLGHLAEA
YSAFVATYQPILDELRRDHLAGVSEQDAFLLRILLIHDYRRLLLRDPELPEVLLPANWPGQQSRLLCKELYKRLEPLASR
HLDQQLCLADGRVPEEDLSLPERFPQNDPLSA
>Mature_352_residues
MLNTGIQNDTRHQVQSKSSTGRHRSEPFPQRPSPAYLVSTAIQSRLNEFRQQRRVQAGSLIITVFGDAILPRGGRIWLGS
LIRLLEPLELNERLIRTSVFRLVKEEWLRTETIGRRADYVLTPSGRRRFEEASRHIYASDAPLWDRRWRLILVVGDLDPK
LREQVRRALFWQGFGALGADCFVHPSAELSSVLDTLITEGLSSAIGALMPLFAADSRSAQSASDADLVHRAWDLGHLAEA
YSAFVATYQPILDELRRDHLAGVSEQDAFLLRILLIHDYRRLLLRDPELPEVLLPANWPGQQSRLLCKELYKRLEPLASR
HLDQQLCLADGRVPEEDLSLPERFPQNDPLSA

Specific function: Negative regulator of the paaZ and paaABCDEFGHIJK catabolic operons. Binds the consensus sequence 5'- WWTRTGATTCGYGWT-3'. Binding of paaX is specifically inhibited by phenylacetyl-coenzyme A [H]

COG id: COG3327

COG function: function code K; Phenylacetic acid-responsive transcriptional repressor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787666, Length=308, Percent_Identity=37.6623376623377, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012906
- InterPro:   IPR013225
- InterPro:   IPR011965 [H]

Pfam domain/function: PF07848 PaaX; PF08223 PaaX_C [H]

EC number: NA

Molecular weight: Translated: 40044; Mature: 40044

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTGIQNDTRHQVQSKSSTGRHRSEPFPQRPSPAYLVSTAIQSRLNEFRQQRRVQAGSL
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
IITVFGDAILPRGGRIWLGSLIRLLEPLELNERLIRTSVFRLVKEEWLRTETIGRRADYV
EEEEECCHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LTPSGRRRFEEASRHIYASDAPLWDRRWRLILVVGDLDPKLREQVRRALFWQGFGALGAD
ECCCHHHHHHHHHCCEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
CFVHPSAELSSVLDTLITEGLSSAIGALMPLFAADSRSAQSASDADLVHRAWDLGHLAEA
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
YSAFVATYQPILDELRRDHLAGVSEQDAFLLRILLIHDYRRLLLRDPELPEVLLPANWPG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
QQSRLLCKELYKRLEPLASRHLDQQLCLADGRVPEEDLSLPERFPQNDPLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure
MLNTGIQNDTRHQVQSKSSTGRHRSEPFPQRPSPAYLVSTAIQSRLNEFRQQRRVQAGSL
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
IITVFGDAILPRGGRIWLGSLIRLLEPLELNERLIRTSVFRLVKEEWLRTETIGRRADYV
EEEEECCHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LTPSGRRRFEEASRHIYASDAPLWDRRWRLILVVGDLDPKLREQVRRALFWQGFGALGAD
ECCCHHHHHHHHHCCEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
CFVHPSAELSSVLDTLITEGLSSAIGALMPLFAADSRSAQSASDADLVHRAWDLGHLAEA
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
YSAFVATYQPILDELRRDHLAGVSEQDAFLLRILLIHDYRRLLLRDPELPEVLLPANWPG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
QQSRLLCKELYKRLEPLASRHLDQQLCLADGRVPEEDLSLPERFPQNDPLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]