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The map label for this gene is lipB

Identifier: 71905930

GI number: 71905930

Start: 330474

End: 331091

Strand: Reverse

Name: lipB

Synonym: Daro_0288

Alternate gene names: 71905930

Gene position: 331091-330474 (Counterclockwise)

Preceding gene: 71905931

Following gene: 71905929

Centisome position: 7.36

GC content: 61.17

Gene sequence:

>618_bases
GTGCCTCCTGTCGTCAAACGCCTGGGCCGGGTCGATTACCAACCCACGTTCCAGGCCATGCAGGACTTCACGGCCAGCCG
TACGGCCGAAACCCCGGACGAAATCTGGATCGTCGAGCATCCGCCGGTCTACACCCTCGGCCAGGCCGGCAAGCCGGAAC
ACATCCTGCGCGATGTCGGCATTCCGCTGGTTAAGATCGACCGCGGCGGCCAGGTGACCTACCACGGCCCCGGCCAGGTC
GTGATCTACCTCTTGCTCGACCTTTCACGCCTGAAGATCAAGGTCCGTGAGCTGGTCACCGCCATCGAACAGTCGGTGAT
CGACCTGCTGGCCGAGTACGGCATCACTGCAGAGCGGCGTGACGGTGCTCCCGGCGTCTATGTCGGCGAAGCCAAGATCG
CGGCCCTCGGCCTGCGCATTCGCAATGGTTGCAGCTACCACGGGGTCTCACTCAATGTCGATATGGATCTTTCCCCCTTT
GCGGCGATCAATCCCTGCGGCTATGCTGGCCTGAAGGTCATCCAGACCAAAGACTTCAATATTCCGCTCACCGCTCACGA
GGCTGGCGAGCAACTCTCGCAACACTTGCTACAGCAACTGGACCAGAAAAATGGCTGA

Upstream 100 bases:

>100_bases
CCTGAGCCTGACGTGCACCGTGATTGCCCGCTCCAAGCCACAACTCGACGCGCTCTACACCGACCTCAGTGGTCATCCGA
TGATCAAGGTCGTCCTCTAA

Downstream 100 bases:

>100_bases
AAAAGGCGTCAAACAAAAAGGCGAACTGAAAACCGCGCGCATCCCCATCAAGATCGTGCCGGTCGATACGCCGCTACGCA
AACCGGAGTGGATTCGCGTC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MPPVVKRLGRVDYQPTFQAMQDFTASRTAETPDEIWIVEHPPVYTLGQAGKPEHILRDVGIPLVKIDRGGQVTYHGPGQV
VIYLLLDLSRLKIKVRELVTAIEQSVIDLLAEYGITAERRDGAPGVYVGEAKIAALGLRIRNGCSYHGVSLNVDMDLSPF
AAINPCGYAGLKVIQTKDFNIPLTAHEAGEQLSQHLLQQLDQKNG

Sequences:

>Translated_205_residues
MPPVVKRLGRVDYQPTFQAMQDFTASRTAETPDEIWIVEHPPVYTLGQAGKPEHILRDVGIPLVKIDRGGQVTYHGPGQV
VIYLLLDLSRLKIKVRELVTAIEQSVIDLLAEYGITAERRDGAPGVYVGEAKIAALGLRIRNGCSYHGVSLNVDMDLSPF
AAINPCGYAGLKVIQTKDFNIPLTAHEAGEQLSQHLLQQLDQKNG
>Mature_204_residues
PPVVKRLGRVDYQPTFQAMQDFTASRTAETPDEIWIVEHPPVYTLGQAGKPEHILRDVGIPLVKIDRGGQVTYHGPGQVV
IYLLLDLSRLKIKVRELVTAIEQSVIDLLAEYGITAERRDGAPGVYVGEAKIAALGLRIRNGCSYHGVSLNVDMDLSPFA
AINPCGYAGLKVIQTKDFNIPLTAHEAGEQLSQHLLQQLDQKNG

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=209, Percent_Identity=33.0143540669856, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI87081767, Length=199, Percent_Identity=55.7788944723618, Blast_Score=228, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6323268, Length=168, Percent_Identity=30.3571428571429, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355483, Length=200, Percent_Identity=32.5, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_DECAR (Q47JD4)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_283517.1
- ProteinModelPortal:   Q47JD4
- SMR:   Q47JD4
- STRING:   Q47JD4
- GeneID:   3570174
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_0288
- NMPDR:   fig|159087.4.peg.491
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   GDEIWVV
- BioCyc:   DARO159087:DARO_0288-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 22506; Mature: 22375

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPVVKRLGRVDYQPTFQAMQDFTASRTAETPDEIWIVEHPPVYTLGQAGKPEHILRDVG
CCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCHHHHHHHCC
IPLVKIDRGGQVTYHGPGQVVIYLLLDLSRLKIKVRELVTAIEQSVIDLLAEYGITAERR
CCEEEECCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DGAPGVYVGEAKIAALGLRIRNGCSYHGVSLNVDMDLSPFAAINPCGYAGLKVIQTKDFN
CCCCCEEECCCEEEEEHEEECCCCCCCCEEEEECCCCCCCCEECCCCCCCEEEEEECCCC
IPLTAHEAGEQLSQHLLQQLDQKNG
CEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PPVVKRLGRVDYQPTFQAMQDFTASRTAETPDEIWIVEHPPVYTLGQAGKPEHILRDVG
CHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCHHHHHHHCC
IPLVKIDRGGQVTYHGPGQVVIYLLLDLSRLKIKVRELVTAIEQSVIDLLAEYGITAERR
CCEEEECCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DGAPGVYVGEAKIAALGLRIRNGCSYHGVSLNVDMDLSPFAAINPCGYAGLKVIQTKDFN
CCCCCEEECCCEEEEEHEEECCCCCCCCEEEEECCCCCCCCEECCCCCCCEEEEEECCCC
IPLTAHEAGEQLSQHLLQQLDQKNG
CEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA