Click here to switch to the map view.

The map label for this gene is lipA

Identifier: 71905929

GI number: 71905929

Start: 329537

End: 330481

Strand: Reverse

Name: lipA

Synonym: Daro_0287

Alternate gene names: 71905929

Gene position: 330481-329537 (Counterclockwise)

Preceding gene: 71905930

Following gene: 71905921

Centisome position: 7.34

GC content: 62.75

Gene sequence:

>945_bases
ATGGCTGAAAAAGGCGTCAAACAAAAAGGCGAACTGAAAACCGCGCGCATCCCCATCAAGATCGTGCCGGTCGATACGCC
GCTACGCAAACCGGAGTGGATTCGCGTCAAGGCCGGCAACAGCGCCGGGCGATTCGGCGAGATCAAGTCCATGCTGCGCG
AGAAAAAGCTGCACACCGTCTGCGAAGAAGCGGCCTGCCCCAACATCGGCGAATGCTTCGGCCGCGGCACGGCGACCTTC
ATGATCCTTGGCGACATCTGCACCCGCCGCTGCCCTTTCTGCGACGTTGGCCACGGCCAGCCGCTGCCGCCCAACCCGAA
CGAACCGGCCGAGCTGGCTGATTCGGTGTCGTCGCTAAAGCTGCAGTACGTGGTAATCACCAGCGTCGACCGCGACGACC
TGCGCGATGGCGGCGCCCAGCATTTCGTCGATGTCGTCCGTGCCGTACGCGAGGCCTCGCCCAAGACGACCATCGAAACC
CTGGTCCCCGACTTCCGTGGCCGCATGGACATCGCCATCGACATTCTCGGCAATGGCCTGCCCGATGTGCTGAACCACAA
CATGGAAACCGTGCCCCGCCTGTACAAGCAAGCCCGTCCCGGTGCCGACTACGCCCATTCGCTGGCCCTGATGAAGCAGT
TCAAGGCACGCTACCCCGACGTCAAGACCAAGTCCGGCCTGATGGTCGGCCTCGGCGAGACCGACGAGGAAATCCTCGAA
GTCATGCGTGACCTGCGGGCCAACGATGTCGAAATGCTGACTATCGGCCAATACCTGGCCCCGTCAGGCCACCACCTGCC
GGTCAGCCGCTACGTCCATCCCGACACTTTCAAAATGTTCGAGGAAGAAGCCAAAAAGATGGGCTTCTCCGGTGCGGCCT
GCGCCCCGATGGTGCGCTCGAGCTACTGGGCTGACCAGCAGGCCCATAGCGCTGGCGTCGCCTGA

Upstream 100 bases:

>100_bases
CTGAAGGTCATCCAGACCAAAGACTTCAATATTCCGCTCACCGCTCACGAGGCTGGCGAGCAACTCTCGCAACACTTGCT
ACAGCAACTGGACCAGAAAA

Downstream 100 bases:

>100_bases
TCACAGCGCAACCCCCAAAAGCAAAGGGCAGCACCGAAAGGTGCTGCCCTTTTTGTATTAGCCGACCTTACCTGACATTG
GCTGGCGGGGGCGGTGGAGA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MAEKGVKQKGELKTARIPIKIVPVDTPLRKPEWIRVKAGNSAGRFGEIKSMLREKKLHTVCEEAACPNIGECFGRGTATF
MILGDICTRRCPFCDVGHGQPLPPNPNEPAELADSVSSLKLQYVVITSVDRDDLRDGGAQHFVDVVRAVREASPKTTIET
LVPDFRGRMDIAIDILGNGLPDVLNHNMETVPRLYKQARPGADYAHSLALMKQFKARYPDVKTKSGLMVGLGETDEEILE
VMRDLRANDVEMLTIGQYLAPSGHHLPVSRYVHPDTFKMFEEEAKKMGFSGAACAPMVRSSYWADQQAHSAGVA

Sequences:

>Translated_314_residues
MAEKGVKQKGELKTARIPIKIVPVDTPLRKPEWIRVKAGNSAGRFGEIKSMLREKKLHTVCEEAACPNIGECFGRGTATF
MILGDICTRRCPFCDVGHGQPLPPNPNEPAELADSVSSLKLQYVVITSVDRDDLRDGGAQHFVDVVRAVREASPKTTIET
LVPDFRGRMDIAIDILGNGLPDVLNHNMETVPRLYKQARPGADYAHSLALMKQFKARYPDVKTKSGLMVGLGETDEEILE
VMRDLRANDVEMLTIGQYLAPSGHHLPVSRYVHPDTFKMFEEEAKKMGFSGAACAPMVRSSYWADQQAHSAGVA
>Mature_313_residues
AEKGVKQKGELKTARIPIKIVPVDTPLRKPEWIRVKAGNSAGRFGEIKSMLREKKLHTVCEEAACPNIGECFGRGTATFM
ILGDICTRRCPFCDVGHGQPLPPNPNEPAELADSVSSLKLQYVVITSVDRDDLRDGGAQHFVDVVRAVREASPKTTIETL
VPDFRGRMDIAIDILGNGLPDVLNHNMETVPRLYKQARPGADYAHSLALMKQFKARYPDVKTKSGLMVGLGETDEEILEV
MRDLRANDVEMLTIGQYLAPSGHHLPVSRYVHPDTFKMFEEEAKKMGFSGAACAPMVRSSYWADQQAHSAGVA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=286, Percent_Identity=44.4055944055944, Blast_Score=247, Evalue=9e-66,
Organism=Homo sapiens, GI37577164, Length=249, Percent_Identity=44.9799196787149, Blast_Score=215, Evalue=4e-56,
Organism=Escherichia coli, GI1786846, Length=308, Percent_Identity=62.3376623376623, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32564533, Length=251, Percent_Identity=44.2231075697211, Blast_Score=217, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=43.7062937062937, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=45.1388888888889, Blast_Score=248, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_DECAR (Q47JD5)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_283516.1
- ProteinModelPortal:   Q47JD5
- STRING:   Q47JD5
- GeneID:   3570173
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_0287
- NMPDR:   fig|159087.4.peg.490
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   DARO159087:DARO_0287-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 34628; Mature: 34497

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKGVKQKGELKTARIPIKIVPVDTPLRKPEWIRVKAGNSAGRFGEIKSMLREKKLHTV
CCCCCCCCCCCCEEEECCEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
CEEAACPNIGECFGRGTATFMILGDICTRRCPFCDVGHGQPLPPNPNEPAELADSVSSLK
HHHHCCCCHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHE
LQYVVITSVDRDDLRDGGAQHFVDVVRAVREASPKTTIETLVPDFRGRMDIAIDILGNGL
EEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCC
PDVLNHNMETVPRLYKQARPGADYAHSLALMKQFKARYPDVKTKSGLMVGLGETDEEILE
HHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHH
VMRDLRANDVEMLTIGQYLAPSGHHLPVSRYVHPDTFKMFEEEAKKMGFSGAACAPMVRS
HHHHHCCCCEEEEEECHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
SYWADQQAHSAGVA
HHHCCHHHHHCCCC
>Mature Secondary Structure 
AEKGVKQKGELKTARIPIKIVPVDTPLRKPEWIRVKAGNSAGRFGEIKSMLREKKLHTV
CCCCCCCCCCCEEEECCEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
CEEAACPNIGECFGRGTATFMILGDICTRRCPFCDVGHGQPLPPNPNEPAELADSVSSLK
HHHHCCCCHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHE
LQYVVITSVDRDDLRDGGAQHFVDVVRAVREASPKTTIETLVPDFRGRMDIAIDILGNGL
EEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCC
PDVLNHNMETVPRLYKQARPGADYAHSLALMKQFKARYPDVKTKSGLMVGLGETDEEILE
HHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHH
VMRDLRANDVEMLTIGQYLAPSGHHLPVSRYVHPDTFKMFEEEAKKMGFSGAACAPMVRS
HHHHHCCCCEEEEEECHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
SYWADQQAHSAGVA
HHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA