Click here to switch to the map view.

The map label for this gene is cysI

Identifier: 71891947

GI number: 71891947

Start: 210564

End: 212291

Strand: Direct

Name: cysI

Synonym: BPEN_164

Alternate gene names: 71891947

Gene position: 210564-212291 (Clockwise)

Preceding gene: 71891946

Following gene: 71891948

Centisome position: 26.6

GC content: 33.62

Gene sequence:

>1728_bases
ATGAACAGAAAATATGATAATAACAATGATAAAAATATACCCTTATTATCCGATAATGAGCGAATCAAAAAAGAAAGCAA
CTTCCTAAGAGGTACTATTGCTCAAAATTTAGACAATAACTTAACCGGTGGATTTAATACAGAAGATGCTCAATTAATCA
GATTTCATGGCATGTATCAACAGGATGATCGCGATGTACGTGTAGAACGAGCAAATCAAAAATTAGAACCACTAATTAAT
ATGATGTTACGTTGTCGTTTACCGGGTGGCGTGATTATGCCACAACAATGGTTGGCTATCGATGATTTTTCAGAAAAATA
TACTTTATATGGCACAATACGTCTTACTACTCGACAAACCTTTCAACTGCATGGTTTATTAAAACCTAACTTGAAAAATG
TGCATCGTTTATTGAATAAATTGGGATTAGATTCTATAGCTACTGCTGGAGATGTAAATCGTAATGTGATATGCACAGCT
AATCCCATGGAATCAACATTACATTATCAAGTATGGGAATTGGCAAAAAGTATTTCTTCATATTTACTGCCAAAATCTAA
TGCATATGCAGAAATTTGGTTAGATTCAAAAAAAACAGAATCTACAGATTCTGAGCCAATTTTAAGCGCTACTTATTTAC
CTCGTAAATTTAAAATAGCAATTGCGATACCACCTATAAATGATGTAGATGTTCATGCTAACGATCTTAGTTTTATTGCT
ATCAAAAGCGAGAACACTGACCAAATAATTGGTTTCAATGTATTAGTAGGCGGAGGATTAGCAATGACTTACGGAGATAT
TACCACATATCCTCGTAAAGCTAGCGCATTCGGATATATTTCTACAAAAGATGTTTTAAAAATTGCAGAAACAGTAGTTA
CAGTACAAAGAGATTGGGGAAATAGATCTGACCGTAGACATGCTAAAACAAAGTATACTTTGACGCGCGTTGGAGTGACA
ACATTTAAATCAGAAGTTGAACGTCGTTCTGGAGTACAATTTCATCCAATTCGTCCTTATGTATTTACGGATAGAGGAGA
TCGATTTGGATGGGTGCAGGGTATTGATGATTATTGGCATCTTACTTTATTTATAGAAAACGGAAGAATATCAAATAATG
ATCCCCATAAGCTCTTAAAACGTGGGATTGCAGAAGTTGCGCAAATACATTCAGGTTCTTTTAGATTGACTGCAAATCAA
AATTTAATTATTTCTGGAGTATCTAAGGACAATAAATTAATGATTGAAAGTACATTGAGAAAATATGGTGTAATCAATGA
TGATATTACCCCGCAACGAAAAGCATCTATGGCATGTGTAGCGTTTCCTACTTGTCCATTAGCGATGGCAGAAGCAGAAC
GATTTTTACCAAAATTTGTTACTAAAATAGAGCATATCATGTCTAAATATAGATTGGAAAAAGATGCTATTATTTTACGA
GTAACAGGTTGCCCTAACAGTTGTGCTCGCGCGATGTTATCAGAAATTGGATTAACAGGAAGATCAATTGGGCGTTATAA
TCTTTATTTAGGAGGTAATAATATCGGTACGCGTATTCCTCGATTGTACAAAGAAAATATTACGGAAAATGATATTTTAA
ATATTCTTGATACGACTATTAGTCGTTGGGCACAAGAGAGAAATAGTCAAGAATCTTATGGAGATTATGTAGTAAGATCT
GGGATAGTTAATGCTGTTATAAATTCTGAAAAAGATTTTTATGAATAA

Upstream 100 bases:

>100_bases
TTATGTTAGCATCTAAATACGGGAGTATGGGTTTAGATAAAGCGAATGAATTTTGGCATAACATGCGGATACAACATCGT
TATCAGAGAGATATTTACTA

Downstream 100 bases:

>100_bases
ACTATTAAATGTTATTAATCATTGCTGGACTGTTACAGAATTCAATTCATTAGACATTAATGAACAAAAATTGGTTTTAG
TTAAAATAAATCGATATTTG

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MNRKYDNNNDKNIPLLSDNERIKKESNFLRGTIAQNLDNNLTGGFNTEDAQLIRFHGMYQQDDRDVRVERANQKLEPLIN
MMLRCRLPGGVIMPQQWLAIDDFSEKYTLYGTIRLTTRQTFQLHGLLKPNLKNVHRLLNKLGLDSIATAGDVNRNVICTA
NPMESTLHYQVWELAKSISSYLLPKSNAYAEIWLDSKKTESTDSEPILSATYLPRKFKIAIAIPPINDVDVHANDLSFIA
IKSENTDQIIGFNVLVGGGLAMTYGDITTYPRKASAFGYISTKDVLKIAETVVTVQRDWGNRSDRRHAKTKYTLTRVGVT
TFKSEVERRSGVQFHPIRPYVFTDRGDRFGWVQGIDDYWHLTLFIENGRISNNDPHKLLKRGIAEVAQIHSGSFRLTANQ
NLIISGVSKDNKLMIESTLRKYGVINDDITPQRKASMACVAFPTCPLAMAEAERFLPKFVTKIEHIMSKYRLEKDAIILR
VTGCPNSCARAMLSEIGLTGRSIGRYNLYLGGNNIGTRIPRLYKENITENDILNILDTTISRWAQERNSQESYGDYVVRS
GIVNAVINSEKDFYE

Sequences:

>Translated_575_residues
MNRKYDNNNDKNIPLLSDNERIKKESNFLRGTIAQNLDNNLTGGFNTEDAQLIRFHGMYQQDDRDVRVERANQKLEPLIN
MMLRCRLPGGVIMPQQWLAIDDFSEKYTLYGTIRLTTRQTFQLHGLLKPNLKNVHRLLNKLGLDSIATAGDVNRNVICTA
NPMESTLHYQVWELAKSISSYLLPKSNAYAEIWLDSKKTESTDSEPILSATYLPRKFKIAIAIPPINDVDVHANDLSFIA
IKSENTDQIIGFNVLVGGGLAMTYGDITTYPRKASAFGYISTKDVLKIAETVVTVQRDWGNRSDRRHAKTKYTLTRVGVT
TFKSEVERRSGVQFHPIRPYVFTDRGDRFGWVQGIDDYWHLTLFIENGRISNNDPHKLLKRGIAEVAQIHSGSFRLTANQ
NLIISGVSKDNKLMIESTLRKYGVINDDITPQRKASMACVAFPTCPLAMAEAERFLPKFVTKIEHIMSKYRLEKDAIILR
VTGCPNSCARAMLSEIGLTGRSIGRYNLYLGGNNIGTRIPRLYKENITENDILNILDTTISRWAQERNSQESYGDYVVRS
GIVNAVINSEKDFYE
>Mature_575_residues
MNRKYDNNNDKNIPLLSDNERIKKESNFLRGTIAQNLDNNLTGGFNTEDAQLIRFHGMYQQDDRDVRVERANQKLEPLIN
MMLRCRLPGGVIMPQQWLAIDDFSEKYTLYGTIRLTTRQTFQLHGLLKPNLKNVHRLLNKLGLDSIATAGDVNRNVICTA
NPMESTLHYQVWELAKSISSYLLPKSNAYAEIWLDSKKTESTDSEPILSATYLPRKFKIAIAIPPINDVDVHANDLSFIA
IKSENTDQIIGFNVLVGGGLAMTYGDITTYPRKASAFGYISTKDVLKIAETVVTVQRDWGNRSDRRHAKTKYTLTRVGVT
TFKSEVERRSGVQFHPIRPYVFTDRGDRFGWVQGIDDYWHLTLFIENGRISNNDPHKLLKRGIAEVAQIHSGSFRLTANQ
NLIISGVSKDNKLMIESTLRKYGVINDDITPQRKASMACVAFPTCPLAMAEAERFLPKFVTKIEHIMSKYRLEKDAIILR
VTGCPNSCARAMLSEIGLTGRSIGRYNLYLGGNNIGTRIPRLYKENITENDILNILDTTISRWAQERNSQESYGDYVVRS
GIVNAVINSEKDFYE

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=560, Percent_Identity=61.4285714285714, Blast_Score=750, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=588, Percent_Identity=43.3673469387755, Blast_Score=512, Evalue=1e-146,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_BLOPB (Q493N3)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277677.1
- ProteinModelPortal:   Q493N3
- SMR:   Q493N3
- STRING:   Q493N3
- GeneID:   3563110
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_164
- eggNOG:   COG0155
- HOGENOM:   HBG626671
- OMA:   ESERVFP
- ProtClustDB:   PRK13504
- BioCyc:   CBLO291272:BPEN_164-MONOMER
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 65261; Mature: 65261

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKYDNNNDKNIPLLSDNERIKKESNFLRGTIAQNLDNNLTGGFNTEDAQLIRFHGMYQ
CCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
QDDRDVRVERANQKLEPLINMMLRCRLPGGVIMPQQWLAIDDFSEKYTLYGTIRLTTRQT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEECCCCCEEEEEEEEEEEECE
FQLHGLLKPNLKNVHRLLNKLGLDSIATAGDVNRNVICTANPMESTLHYQVWELAKSISS
EEEECCCCCCHHHHHHHHHHHCCCHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
YLLPKSNAYAEIWLDSKKTESTDSEPILSATYLPRKFKIAIAIPPINDVDVHANDLSFIA
HCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCEEECCCEEEEE
IKSENTDQIIGFNVLVGGGLAMTYGDITTYPRKASAFGYISTKDVLKIAETVVTVQRDWG
EECCCCCEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
NRSDRRHAKTKYTLTRVGVTTFKSEVERRSGVQFHPIRPYVFTDRGDRFGWVQGIDDYWH
CCCCCHHHHHEEEEEEHHHHHHHHHHHHHCCCEEECCCCEEECCCCCCCCCCCCCCCEEE
LTLFIENGRISNNDPHKLLKRGIAEVAQIHSGSFRLTANQNLIISGVSKDNKLMIESTLR
EEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCEEEHHHHHH
KYGVINDDITPQRKASMACVAFPTCPLAMAEAERFLPKFVTKIEHIMSKYRLEKDAIILR
HCCCCCCCCCCCHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VTGCPNSCARAMLSEIGLTGRSIGRYNLYLGGNNIGTRIPRLYKENITENDILNILDTTI
EECCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
SRWAQERNSQESYGDYVVRSGIVNAVINSEKDFYE
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MNRKYDNNNDKNIPLLSDNERIKKESNFLRGTIAQNLDNNLTGGFNTEDAQLIRFHGMYQ
CCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
QDDRDVRVERANQKLEPLINMMLRCRLPGGVIMPQQWLAIDDFSEKYTLYGTIRLTTRQT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEECCCCCEEEEEEEEEEEECE
FQLHGLLKPNLKNVHRLLNKLGLDSIATAGDVNRNVICTANPMESTLHYQVWELAKSISS
EEEECCCCCCHHHHHHHHHHHCCCHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
YLLPKSNAYAEIWLDSKKTESTDSEPILSATYLPRKFKIAIAIPPINDVDVHANDLSFIA
HCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCEEECCCEEEEE
IKSENTDQIIGFNVLVGGGLAMTYGDITTYPRKASAFGYISTKDVLKIAETVVTVQRDWG
EECCCCCEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
NRSDRRHAKTKYTLTRVGVTTFKSEVERRSGVQFHPIRPYVFTDRGDRFGWVQGIDDYWH
CCCCCHHHHHEEEEEEHHHHHHHHHHHHHCCCEEECCCCEEECCCCCCCCCCCCCCCEEE
LTLFIENGRISNNDPHKLLKRGIAEVAQIHSGSFRLTANQNLIISGVSKDNKLMIESTLR
EEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCEEEHHHHHH
KYGVINDDITPQRKASMACVAFPTCPLAMAEAERFLPKFVTKIEHIMSKYRLEKDAIILR
HCCCCCCCCCCCHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VTGCPNSCARAMLSEIGLTGRSIGRYNLYLGGNNIGTRIPRLYKENITENDILNILDTTI
EECCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
SRWAQERNSQESYGDYVVRSGIVNAVINSEKDFYE
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA