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The map label for this gene is dksA [H]

Identifier: 71891937

GI number: 71891937

Start: 191027

End: 191491

Strand: Direct

Name: dksA [H]

Synonym: BPEN_154

Alternate gene names: 71891937

Gene position: 191027-191491 (Clockwise)

Preceding gene: 71891935

Following gene: 71891938

Centisome position: 24.13

GC content: 30.97

Gene sequence:

>465_bases
ATGTCAACAAAATCACCGAGCAGTCGTAGACGTTCTTCTTTGAATGTATTAATAATTGCTGGAGTTACACCATATCAACG
AAAACCTAATGAAGAATATATGAGTTCTGATCAACTTTTACATTTTAAACGTATCCTTAAAACATGGAAAAATCAACTCA
AAAAAGATTTAGGATATTTTGCGTTATGTGCGCAAGATCAGGTATCTATCAATTTTCCAGATCCAGTAGATAGAGCGGTG
CAAGAAGAAGAATTCAATATTAAATTACGTAATCGAGATAGAGAATATAAACTTGTTAAAAAAATTGAAAAAACCTTAAA
AAAAATAGATACAAATGAATTTGGATTTTGTGCTTCATGCGATGTAGAAATCGGCATCAGAAGATTAGAGGCTCGTCCAA
CAGCTGATTTATGTATTGATTGTAAAACTTTAGAAGAAATTAAAGAAAAACAAATGACCGGATAA

Upstream 100 bases:

>100_bases
TCATGTGTTTAAAAATATTGGAGAAATTACATAAAAATAATGAAATTTTAAAGAAAATATTGATATGAATTGCTTTTAAT
ATATATACAGGAGCAAATAT

Downstream 100 bases:

>100_bases
ATTGTTAATAAAACTAAATTTATATGTGCGTTATATGATATAATTCTGGAAAATTAGTTTTTGAATACCGTAGATATAAT
TTTATTATGACTATTGATTT

Product: dosage-dependent dnaK suppressor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSTKSPSSRRRSSLNVLIIAGVTPYQRKPNEEYMSSDQLLHFKRILKTWKNQLKKDLGYFALCAQDQVSINFPDPVDRAV
QEEEFNIKLRNRDREYKLVKKIEKTLKKIDTNEFGFCASCDVEIGIRRLEARPTADLCIDCKTLEEIKEKQMTG

Sequences:

>Translated_154_residues
MSTKSPSSRRRSSLNVLIIAGVTPYQRKPNEEYMSSDQLLHFKRILKTWKNQLKKDLGYFALCAQDQVSINFPDPVDRAV
QEEEFNIKLRNRDREYKLVKKIEKTLKKIDTNEFGFCASCDVEIGIRRLEARPTADLCIDCKTLEEIKEKQMTG
>Mature_153_residues
STKSPSSRRRSSLNVLIIAGVTPYQRKPNEEYMSSDQLLHFKRILKTWKNQLKKDLGYFALCAQDQVSINFPDPVDRAVQ
EEEFNIKLRNRDREYKLVKKIEKTLKKIDTNEFGFCASCDVEIGIRRLEARPTADLCIDCKTLEEIKEKQMTG

Specific function: Dosage-dependent suppressor of a dnaK deletion mutation. It suppressed not only the temperature-sensitive growth but also the filamentous phenotype of the dnaK deletion strain, while the defect of lambda growth is not suppressed [H]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786338, Length=147, Percent_Identity=66.6666666666667, Blast_Score=210, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012784
- InterPro:   IPR020460
- InterPro:   IPR000962
- InterPro:   IPR020458 [H]

Pfam domain/function: PF01258 zf-dskA_traR [H]

EC number: NA

Molecular weight: Translated: 17933; Mature: 17802

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKSPSSRRRSSLNVLIIAGVTPYQRKPNEEYMSSDQLLHFKRILKTWKNQLKKDLGYF
CCCCCCCCHHHCCCCEEEEECCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCE
ALCAQDQVSINFPDPVDRAVQEEEFNIKLRNRDREYKLVKKIEKTLKKIDTNEFGFCASC
EEECCCCEEECCCCHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEC
DVEIGIRRLEARPTADLCIDCKTLEEIKEKQMTG
CHHHHHHHHCCCCCHHHEECHHHHHHHHHHHCCC
>Mature Secondary Structure 
STKSPSSRRRSSLNVLIIAGVTPYQRKPNEEYMSSDQLLHFKRILKTWKNQLKKDLGYF
CCCCCCCHHHCCCCEEEEECCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCE
ALCAQDQVSINFPDPVDRAVQEEEFNIKLRNRDREYKLVKKIEKTLKKIDTNEFGFCASC
EEECCCCEEECCCCHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEC
DVEIGIRRLEARPTADLCIDCKTLEEIKEKQMTG
CHHHHHHHHCCCCCHHHEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]