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The map label for this gene is trmB [H]

Identifier: 66767346

GI number: 66767346

Start: 1226796

End: 1227581

Strand: Reverse

Name: trmB [H]

Synonym: XC_1014

Alternate gene names: 66767346

Gene position: 1227581-1226796 (Counterclockwise)

Preceding gene: 66767347

Following gene: 66767345

Centisome position: 23.84

GC content: 67.56

Gene sequence:

>786_bases
ATGACTGATCCTTTCACCAGCGACGGCGCCAAGATGCCGCCCAAGCCCTTCACCATCGAAGAAGGTCGCCGCCAGGTGCG
CAGCTTCGTGTTGCGCCAGGGCCGCTTTACGCCGGCGCAGCAGCGCGCGTTCGACGAGCTGTGGCCGCGCTTCGGTCTGG
ACTACACCGGCGCCCCGCGTGACCTGGACGCCGTGTTCGGCCGCCCCGCACCCAAGGTGCTGGAGATCGGTTTCGGCAAT
GGTGCGGCCTTGCGCTTCGCAGCCCAGCACGACCCTGCGCGCGACTACATCGGCATCGAGGTGCATGCCCCCGGTGTCGG
GCGGCTGCTCAATGCGCTGGACGATGACGGCGCCACGCATGTGCGCCTGTATCACCACGATGCAGTGGAAGTGCTCGAGC
GCGAGATCGCCGACGGCGCGCTGGATGAAGTGCGCATCTATTTCCCGGACCCGTGGCACAAGAAGCGCCACAACAAGCGC
CGCCTGGTGCAGCCGGCGTTCGCGCAGTTGCTGGTGCGCAAGCTGCGCGATGGCGGCCGCTTGCACGCGGCCACCGACTG
GGCCGACTACGCCGAACAGATGTGGGACGTGCTCGACGCCACAGAAGGCCTGGTGAATCGCGCCGGCCCACGCGGCCACG
TCGCACGCCCCGCCTGGCGCCCACAAACCCATTTCGAGACCCGTGGCCAGAAGCTGGGCCATGGCGTGTGGGATTTGCTG
TATGACCGGGAATCGGGAATGGAGAATCGGGAATCGCCAGGGCAGGCGCCAGCGGCGCCCGGATAA

Upstream 100 bases:

>100_bases
GCGGGCCACGGCGCGCGACGCCTGCTTGGCTTCCATTTTTCCTTTACTCACGCCCCTCTCCCCGCGGGACAGTGGCGTTG
TTTGCCGATGGCTGATTTTC

Downstream 100 bases:

>100_bases
CCTGCACGCGTCAGCGCCATCCGTTCTTGCCATTCCCCATTCCCGACTCCCCATTCCCGGCACCTCATGGACACCGCGCT
AACGCTGACCAACGACATGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTDPFTSDGAKMPPKPFTIEEGRRQVRSFVLRQGRFTPAQQRAFDELWPRFGLDYTGAPRDLDAVFGRPAPKVLEIGFGN
GAALRFAAQHDPARDYIGIEVHAPGVGRLLNALDDDGATHVRLYHHDAVEVLEREIADGALDEVRIYFPDPWHKKRHNKR
RLVQPAFAQLLVRKLRDGGRLHAATDWADYAEQMWDVLDATEGLVNRAGPRGHVARPAWRPQTHFETRGQKLGHGVWDLL
YDRESGMENRESPGQAPAAPG

Sequences:

>Translated_261_residues
MTDPFTSDGAKMPPKPFTIEEGRRQVRSFVLRQGRFTPAQQRAFDELWPRFGLDYTGAPRDLDAVFGRPAPKVLEIGFGN
GAALRFAAQHDPARDYIGIEVHAPGVGRLLNALDDDGATHVRLYHHDAVEVLEREIADGALDEVRIYFPDPWHKKRHNKR
RLVQPAFAQLLVRKLRDGGRLHAATDWADYAEQMWDVLDATEGLVNRAGPRGHVARPAWRPQTHFETRGQKLGHGVWDLL
YDRESGMENRESPGQAPAAPG
>Mature_260_residues
TDPFTSDGAKMPPKPFTIEEGRRQVRSFVLRQGRFTPAQQRAFDELWPRFGLDYTGAPRDLDAVFGRPAPKVLEIGFGNG
AALRFAAQHDPARDYIGIEVHAPGVGRLLNALDDDGATHVRLYHHDAVEVLEREIADGALDEVRIYFPDPWHKKRHNKRR
LVQPAFAQLLVRKLRDGGRLHAATDWADYAEQMWDVLDATEGLVNRAGPRGHVARPAWRPQTHFETRGQKLGHGVWDLLY
DRESGMENRESPGQAPAAPG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=226, Percent_Identity=50.4424778761062, Blast_Score=235, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 29376; Mature: 29245

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPFTSDGAKMPPKPFTIEEGRRQVRSFVLRQGRFTPAQQRAFDELWPRFGLDYTGAPR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC
DLDAVFGRPAPKVLEIGFGNGAALRFAAQHDPARDYIGIEVHAPGVGRLLNALDDDGATH
CHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCE
VRLYHHDAVEVLEREIADGALDEVRIYFPDPWHKKRHNKRRLVQPAFAQLLVRKLRDGGR
EEEEEHHHHHHHHHHHHCCCHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LHAATDWADYAEQMWDVLDATEGLVNRAGPRGHVARPAWRPQTHFETRGQKLGHGVWDLL
EEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YDRESGMENRESPGQAPAAPG
HHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDPFTSDGAKMPPKPFTIEEGRRQVRSFVLRQGRFTPAQQRAFDELWPRFGLDYTGAPR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC
DLDAVFGRPAPKVLEIGFGNGAALRFAAQHDPARDYIGIEVHAPGVGRLLNALDDDGATH
CHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCE
VRLYHHDAVEVLEREIADGALDEVRIYFPDPWHKKRHNKRRLVQPAFAQLLVRKLRDGGR
EEEEEHHHHHHHHHHHHCCCHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LHAATDWADYAEQMWDVLDATEGLVNRAGPRGHVARPAWRPQTHFETRGQKLGHGVWDLL
EEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YDRESGMENRESPGQAPAAPG
HHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA