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The map label for this gene is thiG [H]

Identifier: 66767347

GI number: 66767347

Start: 1227726

End: 1228520

Strand: Reverse

Name: thiG [H]

Synonym: XC_1015

Alternate gene names: 66767347

Gene position: 1228520-1227726 (Counterclockwise)

Preceding gene: 66767348

Following gene: 66767346

Centisome position: 23.86

GC content: 66.79

Gene sequence:

>795_bases
ATGACACTTTCCGCCCCCTCCGATGCGCTGGTGATCGCCGGCAAGCCCTACCGTTCGCGCCTGCTGACCGGCACCGGCAA
GTTCAAGGACCTGGACGAAACCCGCCTGGCCACCGAGGCCGCTGCCGCGCAGATCGTCACCGTGGCCATTCGCCGGGTGA
ACATTGGCCAGGACCCCAACGCGCCCAGCCTGCTCGATGTGCTGCCGCCGGACCGCTACACCCTGCTCCCCAATACCGCC
GGCTGCTACACCGCCGAAGACGCGGTGCGCACCTGCCGGCTGGCCCGTGAGCTGCTGGATGGCCACAACCTCACCAAGCT
GGAAGTGCTGGGCGACGAGCGCACGCTCTATCCGGACGTGGTGCAGACGCTCAAGGCCGCCGAACAACTGGTCGCCGACG
GTTTCGAGGTCATGGTGTACACCTCCGATGACCCGATCCTGGCCAAGCGGCTGGAAGAAATCGGCTGCGTGGCGGTGATG
CCGCTGGCCGCGCCGATCGGCTCGGGCCTGGGCATCCAGAACAAGTACAACCTGCTGGAAATCATCGAAAACGCCAAGGT
GCCGATCATCGTCGACGCCGGCGTGGGCACCGCCTCCGATGCCGCCATTGCGATGGAACTGGGCTGCGACGGCGTGCTGA
TGAACACCGCCATTGCCGGTGCACGCGACCCGATCCTGATGGCCAGCGCGATGCGCAAGGCGATCGAAGCCGGCCGCGAG
GCGTTCCTGGCTGGCCGGATTCCACGCAAGCGCTACGCCTCGGCATCGAGCCCGGTCGATGGCGTGATCGGATGA

Upstream 100 bases:

>100_bases
GCTGCGCGAGGGCGACGTGGTGGAAATCGTGCATGCACTGGGCGGCGGTTGATCGCGGTGATGGCCGGCGCATCCACGCC
CGATCGGTGATAATCGCCGC

Downstream 100 bases:

>100_bases
GCAGGCGCGCCGAGATGGCCTTGCATGCGCGGCCTGCGCCTGGCGCGGGCCACGGCGCGCGACGCCTGCTTGGCTTCCAT
TTTTCCTTTACTCACGCCCC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTLSAPSDALVIAGKPYRSRLLTGTGKFKDLDETRLATEAAAAQIVTVAIRRVNIGQDPNAPSLLDVLPPDRYTLLPNTA
GCYTAEDAVRTCRLARELLDGHNLTKLEVLGDERTLYPDVVQTLKAAEQLVADGFEVMVYTSDDPILAKRLEEIGCVAVM
PLAAPIGSGLGIQNKYNLLEIIENAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAIAGARDPILMASAMRKAIEAGRE
AFLAGRIPRKRYASASSPVDGVIG

Sequences:

>Translated_264_residues
MTLSAPSDALVIAGKPYRSRLLTGTGKFKDLDETRLATEAAAAQIVTVAIRRVNIGQDPNAPSLLDVLPPDRYTLLPNTA
GCYTAEDAVRTCRLARELLDGHNLTKLEVLGDERTLYPDVVQTLKAAEQLVADGFEVMVYTSDDPILAKRLEEIGCVAVM
PLAAPIGSGLGIQNKYNLLEIIENAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAIAGARDPILMASAMRKAIEAGRE
AFLAGRIPRKRYASASSPVDGVIG
>Mature_263_residues
TLSAPSDALVIAGKPYRSRLLTGTGKFKDLDETRLATEAAAAQIVTVAIRRVNIGQDPNAPSLLDVLPPDRYTLLPNTAG
CYTAEDAVRTCRLARELLDGHNLTKLEVLGDERTLYPDVVQTLKAAEQLVADGFEVMVYTSDDPILAKRLEEIGCVAVMP
LAAPIGSGLGIQNKYNLLEIIENAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAIAGARDPILMASAMRKAIEAGREA
FLAGRIPRKRYASASSPVDGVIG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=254, Percent_Identity=50, Blast_Score=222, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 27915; Mature: 27783

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSAPSDALVIAGKPYRSRLLTGTGKFKDLDETRLATEAAAAQIVTVAIRRVNIGQDPN
CCCCCCCCEEEEECCCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
APSLLDVLPPDRYTLLPNTAGCYTAEDAVRTCRLARELLDGHNLTKLEVLGDERTLYPDV
CCCEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
VQTLKAAEQLVADGFEVMVYTSDDPILAKRLEEIGCVAVMPLAAPIGSGLGIQNKYNLLE
HHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCEEEEHHHHHCCCCCCCCCCHHHHH
IIENAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAIAGARDPILMASAMRKAIEAGRE
HHHCCCCCEEEECCCCCCCCCEEEEEECCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHH
AFLAGRIPRKRYASASSPVDGVIG
HHHHCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TLSAPSDALVIAGKPYRSRLLTGTGKFKDLDETRLATEAAAAQIVTVAIRRVNIGQDPN
CCCCCCCEEEEECCCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
APSLLDVLPPDRYTLLPNTAGCYTAEDAVRTCRLARELLDGHNLTKLEVLGDERTLYPDV
CCCEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
VQTLKAAEQLVADGFEVMVYTSDDPILAKRLEEIGCVAVMPLAAPIGSGLGIQNKYNLLE
HHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCEEEEHHHHHCCCCCCCCCCHHHHH
IIENAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAIAGARDPILMASAMRKAIEAGRE
HHHCCCCCEEEECCCCCCCCCEEEEEECCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHH
AFLAGRIPRKRYASASSPVDGVIG
HHHHCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]