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The map label for this gene is rihB [H]

Identifier: 66766647

GI number: 66766647

Start: 359514

End: 360677

Strand: Reverse

Name: rihB [H]

Synonym: XC_0303

Alternate gene names: 66766647

Gene position: 360677-359514 (Counterclockwise)

Preceding gene: 77761081

Following gene: 66766646

Centisome position: 7.01

GC content: 65.64

Gene sequence:

>1164_bases
ATGACCCACTCGCACGCCGCCCCATCCAAACGCCGCGTCATCCTCGAAGACGACATCGACGGCTTCACGCCCGCGCAGTT
GCTGTTGCTGCAGTCGCCGGAGGTCGAGGTGCTCGGCATCAGCGCGGTCAGCGGCAACATCTGGCGCGATGAGGTGATCG
CACACAGCTGCCGCCTGCTGGAACTGGCCGGGCAGCCGCAGATTCTGGTGCATCCCGGCCCGGTGCACCCGCTGCTCAAC
AGCGAGCTGGCCACCGAACGGTGGGAAGCGCTGTACGGCAAGCTGGTGTGGAAAGGTGCATGGACCAAACACTGGGTGGA
TGGCGACACCGTGCAGAGTGCGCCGCGTTATCACGCGCACGACGTGGTGCCCGATCTGCCACTGGGCAACCCCAGCGTGG
TGCGCGCATCCGACGAACCGGCGGCGCTGTTCATGCTGCGCATGGTGCGCCAGTACCCCGGCGAGGTGAGCATCATTGCC
ACTGGCCCATTGACCAACCTGGCCCTCGCGCAGGCCCTGGACCCGGCCTTCGCCACGCTGGCGCGCGAACTGGTCTACAT
GGGCGGCAGCCTCAATCCACGCCAGCAGCGCGACAGCGTGAGCGCGCGCCAATTCGCGCGCGAGTTCGTCAATTCGCCAC
GCCGCGAATTCAACATCCGCTGGGACCCGGAAGCCGCCAGCATCGTCATGCGCGCACCGTGGCGGAAGATCACCATGGTG
CCGGTGGATCCCTCCACCGCCACCGAACTCACCCCGGAGCTATTGGCGCGCATGAGCGCGGCCGACACCCCCATCGGCCA
CGCATTGCGTCGCCGCGAAACCGGCTTTCCGATGTGGGACGAGCTGGCCACTGCGGTGTGGCTGCGCCCCGAGTTGGCGA
CGCACACCGAAACGCTCTACATCGACACCAACACCGCGTTCGACGCAGGCTATGGCGACATTTTGTCGTGGGCGCCGGGC
TATCAGCCTGGACTGGGCGAACAGGCACAGACGGTGGTGCATGCTGTGAATGTGCAGGCGTTTGAGCAGCTATTGGTCGA
TCACCTCACTGCACCGCAATCACCAGCAGTCGGACTGCCGCTCTCCCTTCTCCCATCGGGAGAAGTTGCCCGAAGGGCGG
ATGAGGGTACGTCACCCGATAAAGACGCCGCGCCAGTTGAGTAG

Upstream 100 bases:

>100_bases
GGAGGCTTCACCGATAACGCCTCATCGCGCGCCACGTCCTACAACGCGACTTCCGCTGCATCGCCGCTGCAATACATTTC
CCCTCCATGACGACAGCGCA

Downstream 100 bases:

>100_bases
GTGGCAAGCAAGGTAAACGCATACCTGATACATCTCCAACTAGCGGGGCACCCTCACCCCAACCCCTCTCCCGGCGGGAG
AGGGGCTCGAGCGACACCAC

Product: nucleoside hydrolase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTHSHAAPSKRRVILEDDIDGFTPAQLLLLQSPEVEVLGISAVSGNIWRDEVIAHSCRLLELAGQPQILVHPGPVHPLLN
SELATERWEALYGKLVWKGAWTKHWVDGDTVQSAPRYHAHDVVPDLPLGNPSVVRASDEPAALFMLRMVRQYPGEVSIIA
TGPLTNLALAQALDPAFATLARELVYMGGSLNPRQQRDSVSARQFAREFVNSPRREFNIRWDPEAASIVMRAPWRKITMV
PVDPSTATELTPELLARMSAADTPIGHALRRRETGFPMWDELATAVWLRPELATHTETLYIDTNTAFDAGYGDILSWAPG
YQPGLGEQAQTVVHAVNVQAFEQLLVDHLTAPQSPAVGLPLSLLPSGEVARRADEGTSPDKDAAPVE

Sequences:

>Translated_387_residues
MTHSHAAPSKRRVILEDDIDGFTPAQLLLLQSPEVEVLGISAVSGNIWRDEVIAHSCRLLELAGQPQILVHPGPVHPLLN
SELATERWEALYGKLVWKGAWTKHWVDGDTVQSAPRYHAHDVVPDLPLGNPSVVRASDEPAALFMLRMVRQYPGEVSIIA
TGPLTNLALAQALDPAFATLARELVYMGGSLNPRQQRDSVSARQFAREFVNSPRREFNIRWDPEAASIVMRAPWRKITMV
PVDPSTATELTPELLARMSAADTPIGHALRRRETGFPMWDELATAVWLRPELATHTETLYIDTNTAFDAGYGDILSWAPG
YQPGLGEQAQTVVHAVNVQAFEQLLVDHLTAPQSPAVGLPLSLLPSGEVARRADEGTSPDKDAAPVE
>Mature_386_residues
THSHAAPSKRRVILEDDIDGFTPAQLLLLQSPEVEVLGISAVSGNIWRDEVIAHSCRLLELAGQPQILVHPGPVHPLLNS
ELATERWEALYGKLVWKGAWTKHWVDGDTVQSAPRYHAHDVVPDLPLGNPSVVRASDEPAALFMLRMVRQYPGEVSIIAT
GPLTNLALAQALDPAFATLARELVYMGGSLNPRQQRDSVSARQFAREFVNSPRREFNIRWDPEAASIVMRAPWRKITMVP
VDPSTATELTPELLARMSAADTPIGHALRRRETGFPMWDELATAVWLRPELATHTETLYIDTNTAFDAGYGDILSWAPGY
QPGLGEQAQTVVHAVNVQAFEQLLVDHLTAPQSPAVGLPLSLLPSGEVARRADEGTSPDKDAAPVE

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=342, Percent_Identity=23.0994152046784, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 42451; Mature: 42319

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSHAAPSKRRVILEDDIDGFTPAQLLLLQSPEVEVLGISAVSGNIWRDEVIAHSCRLL
CCCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHE
ELAGQPQILVHPGPVHPLLNSELATERWEALYGKLVWKGAWTKHWVDGDTVQSAPRYHAH
EECCCCEEEEECCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCHH
DVVPDLPLGNPSVVRASDEPAALFMLRMVRQYPGEVSIIATGPLTNLALAQALDPAFATL
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHH
ARELVYMGGSLNPRQQRDSVSARQFAREFVNSPRREFNIRWDPEAASIVMRAPWRKITMV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCEEECCHHHHEEEECCCCEEEEE
PVDPSTATELTPELLARMSAADTPIGHALRRRETGFPMWDELATAVWLRPELATHTETLY
ECCCCCHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHCCCEEEE
IDTNTAFDAGYGDILSWAPGYQPGLGEQAQTVVHAVNVQAFEQLLVDHLTAPQSPAVGLP
EECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LSLLPSGEVARRADEGTSPDKDAAPVE
CEECCCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
THSHAAPSKRRVILEDDIDGFTPAQLLLLQSPEVEVLGISAVSGNIWRDEVIAHSCRLL
CCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHE
ELAGQPQILVHPGPVHPLLNSELATERWEALYGKLVWKGAWTKHWVDGDTVQSAPRYHAH
EECCCCEEEEECCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCHH
DVVPDLPLGNPSVVRASDEPAALFMLRMVRQYPGEVSIIATGPLTNLALAQALDPAFATL
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHH
ARELVYMGGSLNPRQQRDSVSARQFAREFVNSPRREFNIRWDPEAASIVMRAPWRKITMV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCEEECCHHHHEEEECCCCEEEEE
PVDPSTATELTPELLARMSAADTPIGHALRRRETGFPMWDELATAVWLRPELATHTETLY
ECCCCCHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHCCCEEEE
IDTNTAFDAGYGDILSWAPGYQPGLGEQAQTVVHAVNVQAFEQLLVDHLTAPQSPAVGLP
EECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LSLLPSGEVARRADEGTSPDKDAAPVE
CEECCCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA