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The map label for this gene is gatA [H]

Identifier: 66766646

GI number: 66766646

Start: 357917

End: 359377

Strand: Reverse

Name: gatA [H]

Synonym: XC_0302

Alternate gene names: 66766646

Gene position: 359377-357917 (Counterclockwise)

Preceding gene: 66766647

Following gene: 66766645

Centisome position: 6.98

GC content: 71.05

Gene sequence:

>1461_bases
ATGCAACTCCCCGCGCAGTGGCAGGCCGGCATCGATCGCAACACCGAGGTCCTGCGTGGTCACGCGCAGCTGCTCGCCAG
CTTCAACGCACCGCGCCCCGCATCACCGGACACAAGCACTTCGCGCGGGCAGATCGCCTCCATCGTCGGCAGCACGCGCA
GCCAGCCCGCACAGGCGGCGCGCCTGGCCACCCAGACACTGGGCCGCATCAATCAGGCCAACGATGGCCTGCATGCCATC
ACCCGCTTGTTGCCGGCGCGCGCCGCGGCCGATGCCGCGCGCGTGCAGGCCGCGCTGGCCGGCGGCAGCGATGGCGGCGC
GCTGGCCGGCGTGCCGTTCGTGGTCAAGGATCTGTTCGATGTGGCCGGGCAGGTCACCACCGCCGGTGCTGCGCTGCGCG
CACAAAGCGCGCCGGCCACGCGCGATGCGGCCGTGGTGCAGCGCCTGAGCGATGCCGGTGCAGTGCTGGTGGGCACCGCC
AACATGGACGAATTTGCCTACGGCTTTGCCACCGTCAACGCGCATTACGGCACCACCGCCAATCCGCACGACCATCGCCA
TCTGGCCGGCGGCTCGTCCGGTGGCTCGGCCGCCGCAGTGGCGGCTGGATGGGTGCCGTTTGCACTGGGCTCGGACACCA
ATGGCTCGATCCGCGTGCCGGCTGCCCTGTGCGGCGTGTACGGGTTGCGACCGACCCACGGCACGCTGCCGTTGCAGGGC
GTGTTCCCGTTCGTGGATGCGCTGGATGTGGTGGGCCCGTTCGCGACCTCGGTTGCCGACCTGCGCCGCGTCTACGAAGT
GATGCACGGGCACCCGGTACCCGCTTGCAGCGTGGAGACGCTGCGCATCGCGCAATTGGGCGGCTGGTTCCAACGCAACC
TCGATCCCGAGCTGGAGGCCGGCCTCGGCACGCTGCTCACTGCGATCGGCAGCACCACCATCATGGAGTTACCGGACGCC
GAACGCGCGCGCGCCGCCGCGTTCGTGCTCACCGCCGCCGAGGGCGGGCACCGCCATCGCAACGCCCTCAGCACACAGGC
CGCCCAATTCGACCCAGCCACCCGCGACCGCCTGTTGGCCGGCTTGCAATTGCCGGCCAGCGCGGTGGCCGATGCACAGC
AGTTCGCGCACTGGTTCGCGCGGGCCATGCGCACGCTGTGGGACCGCGTGGATGTGTTGATCGCGCCGGCCACACCTGGC
GTGGCGCCGCGCATCGACCAAGAGAGCATCATCATCGATGGCCTGCCGGTCTCGGCGCGCGCCAACCTGGGCATCTTCAC
CCAGCCGCTTGGGCTGGCCGCCTGCCCGGTGCTGGCCGCGCCGCTACCGCGCCCGGGTCGCTTGCCGCTGGGCGTGCAGC
TGATTGCCGCGCCAGGCCGCGAAGACCGCCTGTTCGCTCTGGCCGCGCAACTCGAACGCGATGGGCTGCTCGCGTTCAGC
GCACCGGCGGAGACACGCTGA

Upstream 100 bases:

>100_bases
CAACTAGCGGGGCACCCTCACCCCAACCCCTCTCCCGGCGGGAGAGGGGCTCGAGCGACACCACGCACCCGCACCACTCC
CAGCAACCCAAGGTGACCCC

Downstream 100 bases:

>100_bases
TGCTGCACACCTTGCGCGCACGCCGCGGCATGGTGGTCGCGCCTCACCATCTGGCCGCGCAGGCCGGGCGCGACATCCTG
CGCGCCGGCGGCAACGCCAT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MQLPAQWQAGIDRNTEVLRGHAQLLASFNAPRPASPDTSTSRGQIASIVGSTRSQPAQAARLATQTLGRINQANDGLHAI
TRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFDVAGQVTTAGAALRAQSAPATRDAAVVQRLSDAGAVLVGTA
NMDEFAYGFATVNAHYGTTANPHDHRHLAGGSSGGSAAAVAAGWVPFALGSDTNGSIRVPAALCGVYGLRPTHGTLPLQG
VFPFVDALDVVGPFATSVADLRRVYEVMHGHPVPACSVETLRIAQLGGWFQRNLDPELEAGLGTLLTAIGSTTIMELPDA
ERARAAAFVLTAAEGGHRHRNALSTQAAQFDPATRDRLLAGLQLPASAVADAQQFAHWFARAMRTLWDRVDVLIAPATPG
VAPRIDQESIIIDGLPVSARANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFS
APAETR

Sequences:

>Translated_486_residues
MQLPAQWQAGIDRNTEVLRGHAQLLASFNAPRPASPDTSTSRGQIASIVGSTRSQPAQAARLATQTLGRINQANDGLHAI
TRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFDVAGQVTTAGAALRAQSAPATRDAAVVQRLSDAGAVLVGTA
NMDEFAYGFATVNAHYGTTANPHDHRHLAGGSSGGSAAAVAAGWVPFALGSDTNGSIRVPAALCGVYGLRPTHGTLPLQG
VFPFVDALDVVGPFATSVADLRRVYEVMHGHPVPACSVETLRIAQLGGWFQRNLDPELEAGLGTLLTAIGSTTIMELPDA
ERARAAAFVLTAAEGGHRHRNALSTQAAQFDPATRDRLLAGLQLPASAVADAQQFAHWFARAMRTLWDRVDVLIAPATPG
VAPRIDQESIIIDGLPVSARANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFS
APAETR
>Mature_486_residues
MQLPAQWQAGIDRNTEVLRGHAQLLASFNAPRPASPDTSTSRGQIASIVGSTRSQPAQAARLATQTLGRINQANDGLHAI
TRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFDVAGQVTTAGAALRAQSAPATRDAAVVQRLSDAGAVLVGTA
NMDEFAYGFATVNAHYGTTANPHDHRHLAGGSSGGSAAAVAAGWVPFALGSDTNGSIRVPAALCGVYGLRPTHGTLPLQG
VFPFVDALDVVGPFATSVADLRRVYEVMHGHPVPACSVETLRIAQLGGWFQRNLDPELEAGLGTLLTAIGSTTIMELPDA
ERARAAAFVLTAAEGGHRHRNALSTQAAQFDPATRDRLLAGLQLPASAVADAQQFAHWFARAMRTLWDRVDVLIAPATPG
VAPRIDQESIIIDGLPVSARANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFS
APAETR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=499, Percent_Identity=25.8517034068136, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI222831590, Length=487, Percent_Identity=25.6673511293634, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI166795287, Length=196, Percent_Identity=33.6734693877551, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=419, Percent_Identity=28.6396181384248, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17537465, Length=467, Percent_Identity=26.5524625267666, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=185, Percent_Identity=35.6756756756757, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=447, Percent_Identity=25.2796420581655, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=165, Percent_Identity=38.7878787878788, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=165, Percent_Identity=38.7878787878788, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=155, Percent_Identity=34.1935483870968, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=382, Percent_Identity=31.9371727748691, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323950, Length=414, Percent_Identity=24.8792270531401, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648113, Length=475, Percent_Identity=31.5789473684211, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24652985, Length=479, Percent_Identity=24.6346555323591, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922090, Length=479, Percent_Identity=24.6346555323591, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652981, Length=479, Percent_Identity=24.6346555323591, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652983, Length=479, Percent_Identity=24.6346555323591, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356731, Length=163, Percent_Identity=38.6503067484663, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24644968, Length=245, Percent_Identity=30.6122448979592, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI45550774, Length=201, Percent_Identity=29.3532338308458, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648435, Length=201, Percent_Identity=29.3532338308458, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648437, Length=201, Percent_Identity=29.3532338308458, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161078093, Length=484, Percent_Identity=23.9669421487603, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648441, Length=400, Percent_Identity=23.25, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648439, Length=400, Percent_Identity=23.25, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 50335; Mature: 50335

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLPAQWQAGIDRNTEVLRGHAQLLASFNAPRPASPDTSTSRGQIASIVGSTRSQPAQAA
CCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHH
RLATQTLGRINQANDGLHAITRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
VAGQVTTAGAALRAQSAPATRDAAVVQRLSDAGAVLVGTANMDEFAYGFATVNAHYGTTA
HHCCHHHCCHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHEEEEEEECCCCCC
NPHDHRHLAGGSSGGSAAAVAAGWVPFALGSDTNGSIRVPAALCGVYGLRPTHGTLPLQG
CCCCCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCEECCHHHHHHHCCCCCCCCCCHHH
VFPFVDALDVVGPFATSVADLRRVYEVMHGHPVPACSVETLRIAQLGGWFQRNLDPELEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHCCCHHHHH
GLGTLLTAIGSTTIMELPDAERARAAAFVLTAAEGGHRHRNALSTQAAQFDPATRDRLLA
HHHHHHHHHCCCEEEECCCHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHH
GLQLPASAVADAQQFAHWFARAMRTLWDRVDVLIAPATPGVAPRIDQESIIIDGLPVSAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCEEEECCCCCCC
ANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFS
CCCCEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEC
APAETR
CCCCCC
>Mature Secondary Structure
MQLPAQWQAGIDRNTEVLRGHAQLLASFNAPRPASPDTSTSRGQIASIVGSTRSQPAQAA
CCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHH
RLATQTLGRINQANDGLHAITRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
VAGQVTTAGAALRAQSAPATRDAAVVQRLSDAGAVLVGTANMDEFAYGFATVNAHYGTTA
HHCCHHHCCHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHEEEEEEECCCCCC
NPHDHRHLAGGSSGGSAAAVAAGWVPFALGSDTNGSIRVPAALCGVYGLRPTHGTLPLQG
CCCCCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCEECCHHHHHHHCCCCCCCCCCHHH
VFPFVDALDVVGPFATSVADLRRVYEVMHGHPVPACSVETLRIAQLGGWFQRNLDPELEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHCCCHHHHH
GLGTLLTAIGSTTIMELPDAERARAAAFVLTAAEGGHRHRNALSTQAAQFDPATRDRLLA
HHHHHHHHHCCCEEEECCCHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHH
GLQLPASAVADAQQFAHWFARAMRTLWDRVDVLIAPATPGVAPRIDQESIIIDGLPVSAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCEEEECCCCCCC
ANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFS
CCCCEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEC
APAETR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA