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The map label for this gene is gcvA [H]

Identifier: 66048239

GI number: 66048239

Start: 5956273

End: 5957259

Strand: Direct

Name: gcvA [H]

Synonym: Psyr_5015

Alternate gene names: 66048239

Gene position: 5956273-5957259 (Clockwise)

Preceding gene: 66048236

Following gene: 66048240

Centisome position: 97.74

GC content: 60.69

Gene sequence:

>987_bases
ATGTGTGCTAATTTCTCACTCCGTGATACCAAAATTCGCTCTAAAACAAAAATATCAGTGAGAAGAAAGTACATGAGACG
CAAGATACCCAGCACCACTGCACTGGTCAGTTTCGAGGCAGCCGCCCGCCACGAGAGCTTCACCAAGGCCGCCAATGAGC
TGTCCCTCACGCAAGGCGCTGTGTGTCGGCAAATCGGCGGACTGGAGGAGTTTCTCGGCGTTGAGCTGTTCCGGCGTTCG
CGCAGGGGCGTAAAGCTCACGGAAGCGGGGCTGTCCTATAGTCGTCGTGTGTCCCAGCAACTGGACGCGGTCGAGCGTGA
CACACTGTCGGTCATGGGCCAGCAAGGTGCCAACGCCATCGAGCTCGCCGTGGTGCCGACCTTCGGAACTCAGTGGCTGC
TGCCGCGCCTGAAGGACTTCCAGCACAAGCACCCCGACGTCACGGTCCACCTGACCAACCGCACCCGCCCGTTCCTGTTT
GCCGACACCGACTTCGATGCCGCGATCTACTTTGGCGATGCCGATTGGTCCGGCACCCAGTCGCATCGGCTGATGGGCGA
AAACTCCATGCCGGTCTGCAGCCCCTCCTTTCTTGAAGGTCGCGACAGCCTGGGAGCACGCGAACTGGCCGAATTGCCAC
TGCTGCAACAGACCACCCGGCCCTACGCCTGGCGGCAATGGTTCCACGCACAGGATCTGGATGTAGCGCGCGACATGACC
GGCCCGCGCTACGAGCTGTTCTCCATGCTGGCGCAGGCGGCCATGCACGACATGGGCATCGCCCTGATTCCGCCCTTCCT
CATTCAGCGTGAACTGCAGGAAAGGCGCCTGGTGATCGCAAACACCCGCGCACTGCCCGGCAGTAGCAGTAAGGCCTATT
ACCTGATGATTCCCGAGCGCAAAGTGGAATCGGCCTCGCTGACTGCATTTCGTGACTGGCTGATCGAACAGGCCCAGCGT
TACCGGCTGCCCCAGGAACAAGCCTGA

Upstream 100 bases:

>100_bases
AGCTTGCCTTACCGCTCATGCCAGTTCCTCGCGTTCAGATGAAATCCTGCAGTGATCCTAGTCCTGGCCCATGCCAGCGA
CAAACGAGGTTTTATCAAGC

Downstream 100 bases:

>100_bases
AGCCGTTCAAAGGTAGTCCATAGCGAAAGTCGCCTACAGATATAACCTTATGTCGCAATAAAGAAACTTCCCACTGATCA
AAAAAATCTGGCATACCCCT

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MCANFSLRDTKIRSKTKISVRRKYMRRKIPSTTALVSFEAAARHESFTKAANELSLTQGAVCRQIGGLEEFLGVELFRRS
RRGVKLTEAGLSYSRRVSQQLDAVERDTLSVMGQQGANAIELAVVPTFGTQWLLPRLKDFQHKHPDVTVHLTNRTRPFLF
ADTDFDAAIYFGDADWSGTQSHRLMGENSMPVCSPSFLEGRDSLGARELAELPLLQQTTRPYAWRQWFHAQDLDVARDMT
GPRYELFSMLAQAAMHDMGIALIPPFLIQRELQERRLVIANTRALPGSSSKAYYLMIPERKVESASLTAFRDWLIEQAQR
YRLPQEQA

Sequences:

>Translated_328_residues
MCANFSLRDTKIRSKTKISVRRKYMRRKIPSTTALVSFEAAARHESFTKAANELSLTQGAVCRQIGGLEEFLGVELFRRS
RRGVKLTEAGLSYSRRVSQQLDAVERDTLSVMGQQGANAIELAVVPTFGTQWLLPRLKDFQHKHPDVTVHLTNRTRPFLF
ADTDFDAAIYFGDADWSGTQSHRLMGENSMPVCSPSFLEGRDSLGARELAELPLLQQTTRPYAWRQWFHAQDLDVARDMT
GPRYELFSMLAQAAMHDMGIALIPPFLIQRELQERRLVIANTRALPGSSSKAYYLMIPERKVESASLTAFRDWLIEQAQR
YRLPQEQA
>Mature_328_residues
MCANFSLRDTKIRSKTKISVRRKYMRRKIPSTTALVSFEAAARHESFTKAANELSLTQGAVCRQIGGLEEFLGVELFRRS
RRGVKLTEAGLSYSRRVSQQLDAVERDTLSVMGQQGANAIELAVVPTFGTQWLLPRLKDFQHKHPDVTVHLTNRTRPFLF
ADTDFDAAIYFGDADWSGTQSHRLMGENSMPVCSPSFLEGRDSLGARELAELPLLQQTTRPYAWRQWFHAQDLDVARDMT
GPRYELFSMLAQAAMHDMGIALIPPFLIQRELQERRLVIANTRALPGSSSKAYYLMIPERKVESASLTAFRDWLIEQAQR
YRLPQEQA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=310, Percent_Identity=35.4838709677419, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1786448, Length=283, Percent_Identity=29.3286219081272, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=30.952380952381, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI145693193, Length=309, Percent_Identity=29.126213592233, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI157672245, Length=141, Percent_Identity=35.4609929078014, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=26.3513513513513, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37328; Mature: 37328

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50931 HTH_LYSR ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCANFSLRDTKIRSKTKISVRRKYMRRKIPSTTALVSFEAAARHESFTKAANELSLTQGA
CCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHH
VCRQIGGLEEFLGVELFRRSRRGVKLTEAGLSYSRRVSQQLDAVERDTLSVMGQQGANAI
HHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ELAVVPTFGTQWLLPRLKDFQHKHPDVTVHLTNRTRPFLFADTDFDAAIYFGDADWSGTQ
EEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCEEEEEECCCCCCCC
SHRLMGENSMPVCSPSFLEGRDSLGARELAELPLLQQTTRPYAWRQWFHAQDLDVARDMT
CHHEECCCCCCCCCCHHHCCCCCCCHHHHHHCCHHHHHCCCHHHHHHHCCCCCHHHHHCC
GPRYELFSMLAQAAMHDMGIALIPPFLIQRELQERRLVIANTRALPGSSSKAYYLMIPER
CCHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCC
KVESASLTAFRDWLIEQAQRYRLPQEQA
CCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCANFSLRDTKIRSKTKISVRRKYMRRKIPSTTALVSFEAAARHESFTKAANELSLTQGA
CCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHH
VCRQIGGLEEFLGVELFRRSRRGVKLTEAGLSYSRRVSQQLDAVERDTLSVMGQQGANAI
HHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ELAVVPTFGTQWLLPRLKDFQHKHPDVTVHLTNRTRPFLFADTDFDAAIYFGDADWSGTQ
EEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCEEEEEECCCCCCCC
SHRLMGENSMPVCSPSFLEGRDSLGARELAELPLLQQTTRPYAWRQWFHAQDLDVARDMT
CHHEECCCCCCCCCCHHHCCCCCCCHHHHHHCCHHHHHCCCHHHHHHHCCCCCHHHHHCC
GPRYELFSMLAQAAMHDMGIALIPPFLIQRELQERRLVIANTRALPGSSSKAYYLMIPER
CCHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCC
KVESASLTAFRDWLIEQAQRYRLPQEQA
CCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]