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The map label for this gene is pntAA [H]

Identifier: 66048240

GI number: 66048240

Start: 5957679

End: 5958800

Strand: Direct

Name: pntAA [H]

Synonym: Psyr_5016

Alternate gene names: 66048240

Gene position: 5957679-5958800 (Clockwise)

Preceding gene: 66048239

Following gene: 66048241

Centisome position: 97.77

GC content: 62.92

Gene sequence:

>1122_bases
GTGCACATTGGTGTTCCTCTCGAAACACAAGCGGGCGAAACCCGGGTATCTGCTACCCCGGAAACCATCAAAAAGCTGAT
CAGCCAGGGTCACAGGGTTACGGTGCAAAGCGGGGCGGGCATTCATGCCAGCGTTCCGGACAGTGCCTACGAGGCTGCTG
GCGCAGTGATCGGTGACAGCAACGAGACCTATGCGGCCGAACTGATTCTCAAGGTCGTCGCACCTGACGATGAGGCGCTG
AGCCTGATCAAAAGTGGCTCGGTGGTCGTCGGCATGCTCAACCCCTTCAACGATGAACTGATCGGCAAAATGGCCGCACG
CGGCATTACCGCGTTCGCCCTGGAAGCCGCGCCGCGCACATCGCGCGCGCAAAGCCTTGACGTGCTGTCTTCGCAAGCCA
ACATCGCGGGCTACAAGGCAGTGCTGCTCGCCGCACATCATTACCCGCGCTTCATGCCCATGCTGATGACCGCTGCCGGC
ACCGTCAAAGCTGCCCGGGTGCTGATCCTGGGCGCAGGCGTTGCTGGTCTGCAGGCCATTGCCACCGCCAAAAGGCTGGG
CGCCGTGGTAGAAGCCTCGGATGTGCGTCCGGCAGTAAAGGAACAGATCGAGTCGCTGGGCGCAAAATTCATCGATGTGC
CCTACGAGACCGACGAAGAGCGCGAATGCGCCGAAGGCGTCGGCGGCTACGCGCGGCCCATGCCGGCCAGCTGGATGCAG
CGCCAGGCCGCCGCTGTGCATGAGCGCGCGAAACTGTCGGACATCGTCATCACGACCGCACTCATTCCCGGCCGCAAGGC
ACCGGTGCTGCTCGGCGCAGAAACCGTGGCGCAGATGAAACCAGGCTCGGTGGTGATCGACCTGGCCGCTGCACAGGGCG
GCAACTGCCCGCTGACCGTTGCCGATCAAGTGGTTGTCGAGCACGGCGTGACCATCGTCGGCCACACCAACCTGCCCGCT
CTGGTCGCAGCCGATGCATCGGCGCTCTACGCCCGCAACCTGCTGGACTTCATGAAGCTGCTGTTCGACAAGGACGGCAC
ATTCTCGATCAACCTCGAAGACGACATCGTCGCCGCATGCCTGATGTGCCGCGACGGGCAAGTTGTCCGCAAGAACGGCT
GA

Upstream 100 bases:

>100_bases
CCCTGCTGCACAGGGGTGTGCTGATTGGCCAAGCCAGACGCACCAGCCGTAGTGCACTGGCCTACATACGAACGATAAAA
AATCGCGCAGGAGATTTGTC

Downstream 100 bases:

>100_bases
GGACACACCAATGGAAGAGCTGATTTCCCCCGGCGTCTACAACCTGATCATTTTCGTACTGGCGATTTATGTCGGCTACC
ACGTGGTCTGGAATGTCACG

Product: alanine dehydrogenase/PNT

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MHIGVPLETQAGETRVSATPETIKKLISQGHRVTVQSGAGIHASVPDSAYEAAGAVIGDSNETYAAELILKVVAPDDEAL
SLIKSGSVVVGMLNPFNDELIGKMAARGITAFALEAAPRTSRAQSLDVLSSQANIAGYKAVLLAAHHYPRFMPMLMTAAG
TVKAARVLILGAGVAGLQAIATAKRLGAVVEASDVRPAVKEQIESLGAKFIDVPYETDEERECAEGVGGYARPMPASWMQ
RQAAAVHERAKLSDIVITTALIPGRKAPVLLGAETVAQMKPGSVVIDLAAAQGGNCPLTVADQVVVEHGVTIVGHTNLPA
LVAADASALYARNLLDFMKLLFDKDGTFSINLEDDIVAACLMCRDGQVVRKNG

Sequences:

>Translated_373_residues
MHIGVPLETQAGETRVSATPETIKKLISQGHRVTVQSGAGIHASVPDSAYEAAGAVIGDSNETYAAELILKVVAPDDEAL
SLIKSGSVVVGMLNPFNDELIGKMAARGITAFALEAAPRTSRAQSLDVLSSQANIAGYKAVLLAAHHYPRFMPMLMTAAG
TVKAARVLILGAGVAGLQAIATAKRLGAVVEASDVRPAVKEQIESLGAKFIDVPYETDEERECAEGVGGYARPMPASWMQ
RQAAAVHERAKLSDIVITTALIPGRKAPVLLGAETVAQMKPGSVVIDLAAAQGGNCPLTVADQVVVEHGVTIVGHTNLPA
LVAADASALYARNLLDFMKLLFDKDGTFSINLEDDIVAACLMCRDGQVVRKNG
>Mature_373_residues
MHIGVPLETQAGETRVSATPETIKKLISQGHRVTVQSGAGIHASVPDSAYEAAGAVIGDSNETYAAELILKVVAPDDEAL
SLIKSGSVVVGMLNPFNDELIGKMAARGITAFALEAAPRTSRAQSLDVLSSQANIAGYKAVLLAAHHYPRFMPMLMTAAG
TVKAARVLILGAGVAGLQAIATAKRLGAVVEASDVRPAVKEQIESLGAKFIDVPYETDEERECAEGVGGYARPMPASWMQ
RQAAAVHERAKLSDIVITTALIPGRKAPVLLGAETVAQMKPGSVVIDLAAAQGGNCPLTVADQVVVEHGVTIVGHTNLPA
LVAADASALYARNLLDFMKLLFDKDGTFSINLEDDIVAACLMCRDGQVVRKNG

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=384, Percent_Identity=39.84375, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI122939153, Length=384, Percent_Identity=39.84375, Blast_Score=266, Evalue=3e-71,
Organism=Escherichia coli, GI1787887, Length=371, Percent_Identity=45.0134770889488, Blast_Score=312, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17550456, Length=369, Percent_Identity=43.0894308943089, Blast_Score=295, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 38964; Mature: 38964

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIGVPLETQAGETRVSATPETIKKLISQGHRVTVQSGAGIHASVPDSAYEAAGAVIGDS
CCCCCCCCCCCCCCEECCCHHHHHHHHHCCCEEEEECCCCEEECCCCHHHHHCCCEECCC
NETYAAELILKVVAPDDEALSLIKSGSVVVGMLNPFNDELIGKMAARGITAFALEAAPRT
CCHHHHHEEEHEECCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCC
SRAQSLDVLSSQANIAGYKAVLLAAHHYPRFMPMLMTAAGTVKAARVLILGAGVAGLQAI
CHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCHHHHEEEEEEECCHHHHHHH
ATAKRLGAVVEASDVRPAVKEQIESLGAKFIDVPYETDEERECAEGVGGYARPMPASWMQ
HHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHH
RQAAAVHERAKLSDIVITTALIPGRKAPVLLGAETVAQMKPGSVVIDLAAAQGGNCPLTV
HHHHHHHHHHHHHHEEEEEEECCCCCCCEEECHHHHHHCCCCCEEEEEEECCCCCCCEEH
ADQVVVEHGVTIVGHTNLPALVAADASALYARNLLDFMKLLFDKDGTFSINLEDDIVAAC
HHHHHHHCCCEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
LMCRDGQVVRKNG
HHCCCCEEEECCC
>Mature Secondary Structure
MHIGVPLETQAGETRVSATPETIKKLISQGHRVTVQSGAGIHASVPDSAYEAAGAVIGDS
CCCCCCCCCCCCCCEECCCHHHHHHHHHCCCEEEEECCCCEEECCCCHHHHHCCCEECCC
NETYAAELILKVVAPDDEALSLIKSGSVVVGMLNPFNDELIGKMAARGITAFALEAAPRT
CCHHHHHEEEHEECCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCC
SRAQSLDVLSSQANIAGYKAVLLAAHHYPRFMPMLMTAAGTVKAARVLILGAGVAGLQAI
CHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCHHHHEEEEEEECCHHHHHHH
ATAKRLGAVVEASDVRPAVKEQIESLGAKFIDVPYETDEERECAEGVGGYARPMPASWMQ
HHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHH
RQAAAVHERAKLSDIVITTALIPGRKAPVLLGAETVAQMKPGSVVIDLAAAQGGNCPLTV
HHHHHHHHHHHHHHEEEEEEECCCCCCCEEECHHHHHHCCCCCEEEEEEECCCCCCCEEH
ADQVVVEHGVTIVGHTNLPALVAADASALYARNLLDFMKLLFDKDGTFSINLEDDIVAAC
HHHHHHHCCCEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
LMCRDGQVVRKNG
HHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]