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The map label for this gene is glcB1 [H]

Identifier: 66047924

GI number: 66047924

Start: 5569094

End: 5571271

Strand: Direct

Name: glcB1 [H]

Synonym: Psyr_4700

Alternate gene names: 66047924

Gene position: 5569094-5571271 (Clockwise)

Preceding gene: 66047922

Following gene: 66047926

Centisome position: 91.39

GC content: 60.97

Gene sequence:

>2178_bases
ATGACTGAGTACGTTCAAGTCGGTGACCTGCAAGTCGCCAGAGTCCTGTTCGACTTCGTGCAGAACGAAGCCACCCCCGG
AACCGGCGTCGATGCCACTGCGTTCTGGGCCGGTGCGGACAAACTGATCCACGACCTTGCGCCAAAGAACAAGGCACTGC
TCGCCAAACGTGATGAGTTACAGGCGAAAATCGACGCCTGGCACCAGTCGCATGCCGGGCAGGCTCACGACGCCTCGGCC
TACAAAGCCTTCCTTCAGGAGATCGGCTACCTGCTGCCAGAAGCGGCGGATTTCCAGATCACCACGCAAAACGTCGATGA
AGAAATCGCCACCATGGCTGGCCCGCAACTGGTCGTGCCGGTGATGAACGCCCGGTTTGCGCTCAATGCGTCGAATGCCC
GTTGGGGTTCGCTGTATGACGCGCTCTACGGCACCGACGCCATCAGCGAAGAAGGCGGCGCGCAAAAGGGCAAGGGCTAT
AACAAGGTGCGTGGCGACAAGGTGATTGCTTTCGCTCGTGCCTTTCTCGATCAGGCAGCGCCTCTGTCAGCAGGTTCACA
TGCCGACTCGACAGCTTATAAGCTGATCGACGGCAAGCTGGTCGTCAGTCTCAAGGGTGGCAGCAATACCGGCCTGCACG
ATGACGCCCAACTGATCGGTTTTCAAGGCGATGCCCTGGCGCCGTTTGCCTTGCTGCTCAAGCACAACGGTCTGCACTTC
GAACTGCAGATCGACGCCGCCAGCCCGGTCGGGCAGACCGATCCGGCGGGCGTGAAAGACATCCTCATGGAAGCCGCGCT
GACCACCATCATGGACTGCGAAGATTCCATTGCCGCTGTGGACGCCGACGACAAAGTGGTCGTCTACCGCAACTGGCTGG
GCCTGATGAAGGGCGACCTGGCCGAGTCGGTGTCCAAGGGCGGTGAAACCTTTACCCGCACCATGAACCCGGACCGCGTC
TACACCACGCCAGAGGGCGGCGAAGTCACCCTGCACGGTCGCTCGCTGCTGTTCATTCGCAACGTGGGCCACTTGATGAC
CATCGATGCGATACTCGACAAGCATGGCAACGAAGTGCCAGAAGGCATTCTCGACGGCCTGCTGACCAGTCTCGCCGCCC
TCCACAACCTCAAGGGCAACACCTCGCGCAGCAACAGCCGCAGCGGCTCGATGTACATCGTCAAACCGAAGATGCACGGT
CCGCAGGAAGCTGCATTCACCAACGAACTGTTCGAGCGTATCGAGCAGGTACTGGGCTTGCCGCGTAACACCTTGAAGGT
CGGGATCATGGACGAGGAGCGCCGTACCACGGTCAACCTCAAGGCCTGCATTCAGGCCGCCAGCGAGCGTGTGGTGTTCA
TCAACACCGGCTTCCTCGACCGCACCGGCGACGAAATCCATACCTCCATGGAAGCGGGACCGGTGGTACGCAAGGCACAG
ATGAAAGCCGAGAAGTGGATCACTGCCTACGAGAACTCCAACGTCGACATCGGTCTGAAATGCGGCCTGCAAGGTCGCGC
ACAGATCGGCAAAGGCATGTGGGCCATGCCGGACCTGATGGCCGCCATGCTGGAGCAGAAAATCGCTCATCCACTGGCTG
GCGCCAACACCGCCTGGGTCCCGTCACCTACGGCGGCTGCGCTGCACGCGCTGCATTACCACAAGGTCGACGTGTTCGCC
CGGCAGGCCGAACTGGCGCAGCGCGAGCAAGCCTCGGTGGATGACATTCTGACCATTGCGCTGGCACCGAACACGGACTG
GACGCCGGAAGAAATCCAGAACGAACTGGACAACAACGCGCAGGGCATTCTTGGCTACGTAGTGCGCTGGATCGATCAGG
GCGTGGGGTGTTCGAAAGTGCCGGACATCAATGACATCGGCCTGATGGAAGACCGCGCTACGCTGCGCATCTCCAGCCAG
CACATGGCCAACTGGCTGCGTCATGGTGTCGTCAGCGAAACTCAGGTTCTGGAAAGCCTCAAGCGCATGGCCCCGGTGGT
GGATCGCCAGAACGCCAACGACCCGCTGTATCGCCCGCTGGCACCCGACTTCGACAGCAACATCGCCTTCCAGGCGGCGG
TCGAACTGGTCATCGAAGGTACCCGGCAGCCCAACGGCTACACCGAGCCGGTATTGCACCGCCGCCGTCGGGAGTTCAAG
GCCATCAACGGCCTGTAA

Upstream 100 bases:

>100_bases
TCATGTAGTATCCCGAATTTCGCACTACATGACACCCGGATCAATAACAGAGCGCTCCCCGAGTGTGCTCGACCCTGCTG
AGCAGAGTGAGGCAAGTGCA

Downstream 100 bases:

>100_bases
TGCGCCGCACACCCTCCTTGCGGGTTCTGGTCCTTGCCGGACCAGAACCCGCAAGGGCTCACCGCCTCAACTTATCCCCA
ATTCCCGCTTGACCAGCTTC

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 725; Mature: 724

Protein sequence:

>725_residues
MTEYVQVGDLQVARVLFDFVQNEATPGTGVDATAFWAGADKLIHDLAPKNKALLAKRDELQAKIDAWHQSHAGQAHDASA
YKAFLQEIGYLLPEAADFQITTQNVDEEIATMAGPQLVVPVMNARFALNASNARWGSLYDALYGTDAISEEGGAQKGKGY
NKVRGDKVIAFARAFLDQAAPLSAGSHADSTAYKLIDGKLVVSLKGGSNTGLHDDAQLIGFQGDALAPFALLLKHNGLHF
ELQIDAASPVGQTDPAGVKDILMEAALTTIMDCEDSIAAVDADDKVVVYRNWLGLMKGDLAESVSKGGETFTRTMNPDRV
YTTPEGGEVTLHGRSLLFIRNVGHLMTIDAILDKHGNEVPEGILDGLLTSLAALHNLKGNTSRSNSRSGSMYIVKPKMHG
PQEAAFTNELFERIEQVLGLPRNTLKVGIMDEERRTTVNLKACIQAASERVVFINTGFLDRTGDEIHTSMEAGPVVRKAQ
MKAEKWITAYENSNVDIGLKCGLQGRAQIGKGMWAMPDLMAAMLEQKIAHPLAGANTAWVPSPTAAALHALHYHKVDVFA
RQAELAQREQASVDDILTIALAPNTDWTPEEIQNELDNNAQGILGYVVRWIDQGVGCSKVPDINDIGLMEDRATLRISSQ
HMANWLRHGVVSETQVLESLKRMAPVVDRQNANDPLYRPLAPDFDSNIAFQAAVELVIEGTRQPNGYTEPVLHRRRREFK
AINGL

Sequences:

>Translated_725_residues
MTEYVQVGDLQVARVLFDFVQNEATPGTGVDATAFWAGADKLIHDLAPKNKALLAKRDELQAKIDAWHQSHAGQAHDASA
YKAFLQEIGYLLPEAADFQITTQNVDEEIATMAGPQLVVPVMNARFALNASNARWGSLYDALYGTDAISEEGGAQKGKGY
NKVRGDKVIAFARAFLDQAAPLSAGSHADSTAYKLIDGKLVVSLKGGSNTGLHDDAQLIGFQGDALAPFALLLKHNGLHF
ELQIDAASPVGQTDPAGVKDILMEAALTTIMDCEDSIAAVDADDKVVVYRNWLGLMKGDLAESVSKGGETFTRTMNPDRV
YTTPEGGEVTLHGRSLLFIRNVGHLMTIDAILDKHGNEVPEGILDGLLTSLAALHNLKGNTSRSNSRSGSMYIVKPKMHG
PQEAAFTNELFERIEQVLGLPRNTLKVGIMDEERRTTVNLKACIQAASERVVFINTGFLDRTGDEIHTSMEAGPVVRKAQ
MKAEKWITAYENSNVDIGLKCGLQGRAQIGKGMWAMPDLMAAMLEQKIAHPLAGANTAWVPSPTAAALHALHYHKVDVFA
RQAELAQREQASVDDILTIALAPNTDWTPEEIQNELDNNAQGILGYVVRWIDQGVGCSKVPDINDIGLMEDRATLRISSQ
HMANWLRHGVVSETQVLESLKRMAPVVDRQNANDPLYRPLAPDFDSNIAFQAAVELVIEGTRQPNGYTEPVLHRRRREFK
AINGL
>Mature_724_residues
TEYVQVGDLQVARVLFDFVQNEATPGTGVDATAFWAGADKLIHDLAPKNKALLAKRDELQAKIDAWHQSHAGQAHDASAY
KAFLQEIGYLLPEAADFQITTQNVDEEIATMAGPQLVVPVMNARFALNASNARWGSLYDALYGTDAISEEGGAQKGKGYN
KVRGDKVIAFARAFLDQAAPLSAGSHADSTAYKLIDGKLVVSLKGGSNTGLHDDAQLIGFQGDALAPFALLLKHNGLHFE
LQIDAASPVGQTDPAGVKDILMEAALTTIMDCEDSIAAVDADDKVVVYRNWLGLMKGDLAESVSKGGETFTRTMNPDRVY
TTPEGGEVTLHGRSLLFIRNVGHLMTIDAILDKHGNEVPEGILDGLLTSLAALHNLKGNTSRSNSRSGSMYIVKPKMHGP
QEAAFTNELFERIEQVLGLPRNTLKVGIMDEERRTTVNLKACIQAASERVVFINTGFLDRTGDEIHTSMEAGPVVRKAQM
KAEKWITAYENSNVDIGLKCGLQGRAQIGKGMWAMPDLMAAMLEQKIAHPLAGANTAWVPSPTAAALHALHYHKVDVFAR
QAELAQREQASVDDILTIALAPNTDWTPEEIQNELDNNAQGILGYVVRWIDQGVGCSKVPDINDIGLMEDRATLRISSQH
MANWLRHGVVSETQVLESLKRMAPVVDRQNANDPLYRPLAPDFDSNIAFQAAVELVIEGTRQPNGYTEPVLHRRRREFKA
INGL

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789348, Length=721, Percent_Identity=59.0846047156727, Blast_Score=904, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 78974; Mature: 78843

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYVQVGDLQVARVLFDFVQNEATPGTGVDATAFWAGADKLIHDLAPKNKALLAKRDEL
CCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QAKIDAWHQSHAGQAHDASAYKAFLQEIGYLLPEAADFQITTQNVDEEIATMAGPQLVVP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCEEEEE
VMNARFALNASNARWGSLYDALYGTDAISEEGGAQKGKGYNKVRGDKVIAFARAFLDQAA
ECCCEEEEECCCCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PLSAGSHADSTAYKLIDGKLVVSLKGGSNTGLHDDAQLIGFQGDALAPFALLLKHNGLHF
CCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCEEE
ELQIDAASPVGQTDPAGVKDILMEAALTTIMDCEDSIAAVDADDKVVVYRNWLGLMKGDL
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECHHHHHHHHH
AESVSKGGETFTRTMNPDRVYTTPEGGEVTLHGRSLLFIRNVGHLMTIDAILDKHGNEVP
HHHHHCCCCEEEECCCCCEEEECCCCCEEEEECCEEEEEECCCHHHHHHHHHHHCCCCCH
EGILDGLLTSLAALHNLKGNTSRSNSRSGSMYIVKPKMHGPQEAAFTNELFERIEQVLGL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
PRNTLKVGIMDEERRTTVNLKACIQAASERVVFINTGFLDRTGDEIHTSMEAGPVVRKAQ
CCCCEEEEEECCCCHHEEHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHH
MKAEKWITAYENSNVDIGLKCGLQGRAQIGKGMWAMPDLMAAMLEQKIAHPLAGANTAWV
HHHHHHEEEECCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
PSPTAAALHALHYHKVDVFARQAELAQREQASVDDILTIALAPNTDWTPEEIQNELDNNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCH
QGILGYVVRWIDQGVGCSKVPDINDIGLMEDRATLRISSQHMANWLRHGVVSETQVLESL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCHHHHHHHHH
KRMAPVVDRQNANDPLYRPLAPDFDSNIAFQAAVELVIEGTRQPNGYTEPVLHRRRREFK
HHHCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AINGL
HHCCC
>Mature Secondary Structure 
TEYVQVGDLQVARVLFDFVQNEATPGTGVDATAFWAGADKLIHDLAPKNKALLAKRDEL
CCCEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QAKIDAWHQSHAGQAHDASAYKAFLQEIGYLLPEAADFQITTQNVDEEIATMAGPQLVVP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCEEEEE
VMNARFALNASNARWGSLYDALYGTDAISEEGGAQKGKGYNKVRGDKVIAFARAFLDQAA
ECCCEEEEECCCCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PLSAGSHADSTAYKLIDGKLVVSLKGGSNTGLHDDAQLIGFQGDALAPFALLLKHNGLHF
CCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCEEE
ELQIDAASPVGQTDPAGVKDILMEAALTTIMDCEDSIAAVDADDKVVVYRNWLGLMKGDL
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECHHHHHHHHH
AESVSKGGETFTRTMNPDRVYTTPEGGEVTLHGRSLLFIRNVGHLMTIDAILDKHGNEVP
HHHHHCCCCEEEECCCCCEEEECCCCCEEEEECCEEEEEECCCHHHHHHHHHHHCCCCCH
EGILDGLLTSLAALHNLKGNTSRSNSRSGSMYIVKPKMHGPQEAAFTNELFERIEQVLGL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
PRNTLKVGIMDEERRTTVNLKACIQAASERVVFINTGFLDRTGDEIHTSMEAGPVVRKAQ
CCCCEEEEEECCCCHHEEHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHH
MKAEKWITAYENSNVDIGLKCGLQGRAQIGKGMWAMPDLMAAMLEQKIAHPLAGANTAWV
HHHHHHEEEECCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
PSPTAAALHALHYHKVDVFARQAELAQREQASVDDILTIALAPNTDWTPEEIQNELDNNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCH
QGILGYVVRWIDQGVGCSKVPDINDIGLMEDRATLRISSQHMANWLRHGVVSETQVLESL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCHHHHHHHHH
KRMAPVVDRQNANDPLYRPLAPDFDSNIAFQAAVELVIEGTRQPNGYTEPVLHRRRREFK
HHHCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AINGL
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]