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The map label for this gene is ribH

Identifier: 66047686

GI number: 66047686

Start: 5322339

End: 5322815

Strand: Reverse

Name: ribH

Synonym: Psyr_4459

Alternate gene names: 66047686

Gene position: 5322815-5322339 (Counterclockwise)

Preceding gene: 66047687

Following gene: 66047685

Centisome position: 87.35

GC content: 60.59

Gene sequence:

>477_bases
ATGACCTTGAAGACCATCGAAGGTACCTTCATCGCCCCCCAAGGCCGCTATGCCTTGGTAGTTGGCCGTTTCAACAGCTT
CGTCGTGGAAAGCCTGGTTAGCGGTGCCGTTGATGCGCTGGTACGCCATGGTGTGAGCGAAAGCGACATCACCATCATCC
GTGCGCCGGGTGCGTTCGAGATTCCACTGGTTGTGCAGAAAGTTGCCCAACGCAGCGAGTTCGCGGCCATCGTCGCACTG
GGTGCAGTGATCCGTGGCGGTACTCCGCACTTCGAATACGTGGCAGGCGAGTGCGTCAAGGGCTTGTCCCAGGTGTCCAT
GGAGTTCGGCGTGCCGGTCGCTTTCGGCGTATTGACTGTCGATTCCATCGAGCAGGCCATCGAGCGCTCCGGCACCAAGG
CCGGCAACAAGGGCGCTGAAGCTGCCCTGTCCGCCATCGAAATGGTCAGCCTGCTGTCGCAGCTGGAGGCCAAGTGA

Upstream 100 bases:

>100_bases
GGTTTTTCGCAGGTTTTGGCCTGACAGGCATGATTCGTGGCCTAATATCCCTCAAAGTCCGCCCCAAGGCGGGCTTGCTC
TTTAATACGAGACTCACCGA

Downstream 100 bases:

>100_bases
TTTCCGACGATACCGACCAGTTCAACCCGCGCGACGCCAAATCGCCGGAAATCGCCAAGGGCAAGAGCGCCAAACGTCGC
GAAGCGCGTCAGATGGCAAC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase 1; Lumazine synthase 1; Riboflavin synthase 1 beta chain

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MTLKTIEGTFIAPQGRYALVVGRFNSFVVESLVSGAVDALVRHGVSESDITIIRAPGAFEIPLVVQKVAQRSEFAAIVAL
GAVIRGGTPHFEYVAGECVKGLSQVSMEFGVPVAFGVLTVDSIEQAIERSGTKAGNKGAEAALSAIEMVSLLSQLEAK

Sequences:

>Translated_158_residues
MTLKTIEGTFIAPQGRYALVVGRFNSFVVESLVSGAVDALVRHGVSESDITIIRAPGAFEIPLVVQKVAQRSEFAAIVAL
GAVIRGGTPHFEYVAGECVKGLSQVSMEFGVPVAFGVLTVDSIEQAIERSGTKAGNKGAEAALSAIEMVSLLSQLEAK
>Mature_157_residues
TLKTIEGTFIAPQGRYALVVGRFNSFVVESLVSGAVDALVRHGVSESDITIIRAPGAFEIPLVVQKVAQRSEFAAIVALG
AVIRGGTPHFEYVAGECVKGLSQVSMEFGVPVAFGVLTVDSIEQAIERSGTKAGNKGAEAALSAIEMVSLLSQLEAK

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=156, Percent_Identity=52.5641025641026, Blast_Score=173, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6324429, Length=142, Percent_Identity=29.5774647887324, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB1_PSESM (Q889Q6)

Other databases:

- EMBL:   AE016853
- RefSeq:   NP_790540.1
- ProteinModelPortal:   Q889Q6
- SMR:   Q889Q6
- GeneID:   1182313
- GenomeReviews:   AE016853_GR
- KEGG:   pst:PSPTO_0693
- TIGR:   PSPTO_0693
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- BioCyc:   PSYR223283:PSPTO_0693-MONOMER
- BRENDA:   2.5.1.9
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16520; Mature: 16389

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKTIEGTFIAPQGRYALVVGRFNSFVVESLVSGAVDALVRHGVSESDITIIRAPGAFE
CCCCCCCCCEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
IPLVVQKVAQRSEFAAIVALGAVIRGGTPHFEYVAGECVKGLSQVSMEFGVPVAFGVLTV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
DSIEQAIERSGTKAGNKGAEAALSAIEMVSLLSQLEAK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLKTIEGTFIAPQGRYALVVGRFNSFVVESLVSGAVDALVRHGVSESDITIIRAPGAFE
CCCCCCCCEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
IPLVVQKVAQRSEFAAIVALGAVIRGGTPHFEYVAGECVKGLSQVSMEFGVPVAFGVLTV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
DSIEQAIERSGTKAGNKGAEAALSAIEMVSLLSQLEAK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499