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The map label for this gene is nusB [H]

Identifier: 66047685

GI number: 66047685

Start: 5321845

End: 5322342

Strand: Reverse

Name: nusB [H]

Synonym: Psyr_4458

Alternate gene names: 66047685

Gene position: 5322342-5321845 (Counterclockwise)

Preceding gene: 66047686

Following gene: 66047684

Centisome position: 87.34

GC content: 58.23

Gene sequence:

>498_bases
GTGATTTCCGACGATACCGACCAGTTCAACCCGCGCGACGCCAAATCGCCGGAAATCGCCAAGGGCAAGAGCGCCAAACG
TCGCGAAGCGCGTCAGATGGCAACTCAGGCCCTGTACCAACGGCACATGGCTGGCCACGCGCTGAACGAAATCGAAGCCC
AGTTCCGTGTCGATAATGACTTCAGTAACGTTGACGGCACCTACTTCCGTGAGTTGTTACACGGCGTGGCAACCAACCAG
ACCGAGATCGACACCGCTCTGAAGCCTTGCCTTGACCTGACCATCGAAGAACTCGATCCGATTGAGCTGGCGATATTGCG
CCTGTCCACCTTCGAACTGCTCAAGCGCATCGACGTGCCTTATCGCGTGGTGATCAACGAAGGCATCGAGCTGGCGAAAG
TCTACGGCTCCACTGACGGCCACAAGTTCGTCAATGGCGTGCTGGACAAGCTGGCGCCGCGCCTGCGTGAAGTCGAAGTG
AAGGCCCACAAGCGCTGA

Upstream 100 bases:

>100_bases
AGGCCATCGAGCGCTCCGGCACCAAGGCCGGCAACAAGGGCGCTGAAGCTGCCCTGTCCGCCATCGAAATGGTCAGCCTG
CTGTCGCAGCTGGAGGCCAA

Downstream 100 bases:

>100_bases
TTCGCGCCTGACCTTCATGGGAGAGTTCGAGCTGATCCGCAATTACTTCGCCGCCGCGCCCTGTGCGCAGGCGGGCGAGG
AAGTTGCCCTGGGGATCGGC

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MISDDTDQFNPRDAKSPEIAKGKSAKRREARQMATQALYQRHMAGHALNEIEAQFRVDNDFSNVDGTYFRELLHGVATNQ
TEIDTALKPCLDLTIEELDPIELAILRLSTFELLKRIDVPYRVVINEGIELAKVYGSTDGHKFVNGVLDKLAPRLREVEV
KAHKR

Sequences:

>Translated_165_residues
MISDDTDQFNPRDAKSPEIAKGKSAKRREARQMATQALYQRHMAGHALNEIEAQFRVDNDFSNVDGTYFRELLHGVATNQ
TEIDTALKPCLDLTIEELDPIELAILRLSTFELLKRIDVPYRVVINEGIELAKVYGSTDGHKFVNGVLDKLAPRLREVEV
KAHKR
>Mature_165_residues
MISDDTDQFNPRDAKSPEIAKGKSAKRREARQMATQALYQRHMAGHALNEIEAQFRVDNDFSNVDGTYFRELLHGVATNQ
TEIDTALKPCLDLTIEELDPIELAILRLSTFELLKRIDVPYRVVINEGIELAKVYGSTDGHKFVNGVLDKLAPRLREVEV
KAHKR

Specific function: Involved in the transcription termination process [H]

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family [H]

Homologues:

Organism=Escherichia coli, GI1786618, Length=125, Percent_Identity=52, Blast_Score=134, Evalue=2e-33,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011605
- InterPro:   IPR006027 [H]

Pfam domain/function: PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 18732; Mature: 18732

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISDDTDQFNPRDAKSPEIAKGKSAKRREARQMATQALYQRHMAGHALNEIEAQFRVDND
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
FSNVDGTYFRELLHGVATNQTEIDTALKPCLDLTIEELDPIELAILRLSTFELLKRIDVP
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
YRVVINEGIELAKVYGSTDGHKFVNGVLDKLAPRLREVEVKAHKR
EEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISDDTDQFNPRDAKSPEIAKGKSAKRREARQMATQALYQRHMAGHALNEIEAQFRVDND
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
FSNVDGTYFRELLHGVATNQTEIDTALKPCLDLTIEELDPIELAILRLSTFELLKRIDVP
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
YRVVINEGIELAKVYGSTDGHKFVNGVLDKLAPRLREVEVKAHKR
EEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA