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The map label for this gene is dppA [H]

Identifier: 66047462

GI number: 66047462

Start: 5044772

End: 5046400

Strand: Direct

Name: dppA [H]

Synonym: Psyr_4235

Alternate gene names: 66047462

Gene position: 5044772-5046400 (Clockwise)

Preceding gene: 66047461

Following gene: 66047463

Centisome position: 82.79

GC content: 57.15

Gene sequence:

>1629_bases
ATGCTCAAACACGCGGTCATTCCGTTTCTGGTCGGCGCCAGCTTGCTCGCCAGCGCACCTTTCGCTCACGCGGCATCGAA
TCTGGTGTTCTGTTCCGAGGGCAGCCCAGCGGGCTTCGATCCGGGTCAATACACCACTGGTACTGACTTCGACGCCTCGG
CAGAGACCATCTTCAACCGCCTGAGTCAGTTTGAGCGCGGCGGCACCGCCGTAGTGCCGGGCCTGGCGACCAAATGGGAC
ATCTCCGAAGATGGTCTGACGTACACCTTCCACCTTCGCGAAGGCGTCAAATTCCACACCACGCCGTACTTCAAGCCGAC
CCGTGAATTCAATGCCGATGACGTGCTGTTCACGTTCAATCGCATGATCGACAAGGACATGCCTTACCGCAAAGCGTACC
CGACCGAATTCCCGTACTTCACCGATATGGCGATGGACACCAACATCAAGAAGATCGAGAAGATCGACGATCACACCGTC
AAGTTCGTGCTGGGCATGGTCGACGCCGCGTTCATCCAGAACATGGCGATGAGCTTCGCGTCCATCCAGTCCGCAGAGTA
CGCCGCCAAGCTGCTCAAGGAAGGCAAGCCGGAGCTGATCAATCAGCAGCCGATCGGCACTGGCCCGTTCGTGTTCAAGA
GCTACCAGAAAGACTCCAACATCCGTTACACCGGCAACAAGGATTACTGGAAGCCTGAGGACGTCAAGGTCGACAATCTG
ATCTTCGCCATCACCACCGATGCTTCGGTGCGCATGCAGAAGCTCAAGAAAAACGAATGCCAGATCACCGCCTACCCGCG
TCCTGCCGACCTCGAACCGCTCAAGGCTGACAAGAACCTGAAGATGCCTGATCAGGCGGGCTTCAACCTGGGTTACATCG
CCTACAACGTGATGCCTCAGATCAAGGGCGAAGACCATCCAAACCCGCTGGCTCAGTTGGAAGTGCGTCAGGCGCTGGAC
ATGGCGGTCAACAAGCAGCAGATCATCGACTCCGTTTACCAGGGTGCTGGTCAATTGGCGGTCAACGCCATGCCGCCGAC
CCAGTGGTCCTATGACACCACCATCAAGGACGCCAAGTACGATCCCGAGAAAGCTCGCCAGCTGCTCAAGGAAGCCGGCA
TCAAGGAAGGCACCGAAATCACCCTGTGGGCGATGCCGGTTCAGCGTCCTTACAACCCCAACGCCAAACTGATGGCCGAA
ATGCTCCAGTCCGACTGGAAGAAGATCGGTATCAACGCCAAGATCGTCAGCTATGAATGGGGCGAGTACATCAAGCGCGC
CAAGAATGGCGAGAACGGCGCCATGCTGATTGGTTGGAGCGGTGACAATGGTGACCCGGACAACTGGCTCGGCACCCTGT
TCGGCTGCGACGCCGTCAATGGCAACAACTTTGCCAAATGGTGCGACAAGCCGTTCGACACGCTGATCCATCAGGCCAAG
CAAACGTCCGATCAGGCCGAGCGCACCAAACTGTACAAGCAGGCGCAACACCTCCTGAAAGACGCCGTGCCAATGACGCC
TATTGCGCACTCGACGGTCTATCAACCCATGAGCTCCAAAGTACAGGACTTCAAGATCAGCCCGTTTGGCTTGAACTCCT
TTTACGGTGTGAGCGTGAGCGGCAAGTAA

Upstream 100 bases:

>100_bases
CTCACTGTCTTGCTTTGGGTATGGCCCCTGCATAAGTATCGGACGGATCGGCTCACGAGGTCGTTCCCTCTAATCAAAAA
TGACAACAACTGAGGCAACC

Downstream 100 bases:

>100_bases
AAGCGACTGTTTGCTGCGCCGCTCACCCGGCGCAGCAAACCGCTCTACTTGAACATAAGCCCCGAGCGATGGGTTGAAAC
GTGCCGATAACGGCATTCTT

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MLKHAVIPFLVGASLLASAPFAHAASNLVFCSEGSPAGFDPGQYTTGTDFDASAETIFNRLSQFERGGTAVVPGLATKWD
ISEDGLTYTFHLREGVKFHTTPYFKPTREFNADDVLFTFNRMIDKDMPYRKAYPTEFPYFTDMAMDTNIKKIEKIDDHTV
KFVLGMVDAAFIQNMAMSFASIQSAEYAAKLLKEGKPELINQQPIGTGPFVFKSYQKDSNIRYTGNKDYWKPEDVKVDNL
IFAITTDASVRMQKLKKNECQITAYPRPADLEPLKADKNLKMPDQAGFNLGYIAYNVMPQIKGEDHPNPLAQLEVRQALD
MAVNKQQIIDSVYQGAGQLAVNAMPPTQWSYDTTIKDAKYDPEKARQLLKEAGIKEGTEITLWAMPVQRPYNPNAKLMAE
MLQSDWKKIGINAKIVSYEWGEYIKRAKNGENGAMLIGWSGDNGDPDNWLGTLFGCDAVNGNNFAKWCDKPFDTLIHQAK
QTSDQAERTKLYKQAQHLLKDAVPMTPIAHSTVYQPMSSKVQDFKISPFGLNSFYGVSVSGK

Sequences:

>Translated_542_residues
MLKHAVIPFLVGASLLASAPFAHAASNLVFCSEGSPAGFDPGQYTTGTDFDASAETIFNRLSQFERGGTAVVPGLATKWD
ISEDGLTYTFHLREGVKFHTTPYFKPTREFNADDVLFTFNRMIDKDMPYRKAYPTEFPYFTDMAMDTNIKKIEKIDDHTV
KFVLGMVDAAFIQNMAMSFASIQSAEYAAKLLKEGKPELINQQPIGTGPFVFKSYQKDSNIRYTGNKDYWKPEDVKVDNL
IFAITTDASVRMQKLKKNECQITAYPRPADLEPLKADKNLKMPDQAGFNLGYIAYNVMPQIKGEDHPNPLAQLEVRQALD
MAVNKQQIIDSVYQGAGQLAVNAMPPTQWSYDTTIKDAKYDPEKARQLLKEAGIKEGTEITLWAMPVQRPYNPNAKLMAE
MLQSDWKKIGINAKIVSYEWGEYIKRAKNGENGAMLIGWSGDNGDPDNWLGTLFGCDAVNGNNFAKWCDKPFDTLIHQAK
QTSDQAERTKLYKQAQHLLKDAVPMTPIAHSTVYQPMSSKVQDFKISPFGLNSFYGVSVSGK
>Mature_542_residues
MLKHAVIPFLVGASLLASAPFAHAASNLVFCSEGSPAGFDPGQYTTGTDFDASAETIFNRLSQFERGGTAVVPGLATKWD
ISEDGLTYTFHLREGVKFHTTPYFKPTREFNADDVLFTFNRMIDKDMPYRKAYPTEFPYFTDMAMDTNIKKIEKIDDHTV
KFVLGMVDAAFIQNMAMSFASIQSAEYAAKLLKEGKPELINQQPIGTGPFVFKSYQKDSNIRYTGNKDYWKPEDVKVDNL
IFAITTDASVRMQKLKKNECQITAYPRPADLEPLKADKNLKMPDQAGFNLGYIAYNVMPQIKGEDHPNPLAQLEVRQALD
MAVNKQQIIDSVYQGAGQLAVNAMPPTQWSYDTTIKDAKYDPEKARQLLKEAGIKEGTEITLWAMPVQRPYNPNAKLMAE
MLQSDWKKIGINAKIVSYEWGEYIKRAKNGENGAMLIGWSGDNGDPDNWLGTLFGCDAVNGNNFAKWCDKPFDTLIHQAK
QTSDQAERTKLYKQAQHLLKDAVPMTPIAHSTVYQPMSSKVQDFKISPFGLNSFYGVSVSGK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=533, Percent_Identity=51.9699812382739, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1787551, Length=513, Percent_Identity=33.5282651072125, Blast_Score=331, Evalue=7e-92,
Organism=Escherichia coli, GI1787052, Length=542, Percent_Identity=27.3062730627306, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1787762, Length=552, Percent_Identity=25.7246376811594, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789887, Length=550, Percent_Identity=23.2727272727273, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI87081878, Length=556, Percent_Identity=23.5611510791367, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787495, Length=459, Percent_Identity=22.4400871459695, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789397, Length=521, Percent_Identity=21.8809980806142, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60683; Mature: 60683

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKHAVIPFLVGASLLASAPFAHAASNLVFCSEGSPAGFDPGQYTTGTDFDASAETIFNR
CCCHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LSQFERGGTAVVPGLATKWDISEDGLTYTFHLREGVKFHTTPYFKPTREFNADDVLFTFN
HHHHHCCCCEEECCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCCCCCCCCCHHHHHHH
RMIDKDMPYRKAYPTEFPYFTDMAMDTNIKKIEKIDDHTVKFVLGMVDAAFIQNMAMSFA
HHHCCCCCCCCCCCCCCCCEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIQSAEYAAKLLKEGKPELINQQPIGTGPFVFKSYQKDSNIRYTGNKDYWKPEDVKVDNL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCEECCE
IFAITTDASVRMQKLKKNECQITAYPRPADLEPLKADKNLKMPDQAGFNLGYIAYNVMPQ
EEEEECCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEEECCC
IKGEDHPNPLAQLEVRQALDMAVNKQQIIDSVYQGAGQLAVNAMPPTQWSYDTTIKDAKY
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC
DPEKARQLLKEAGIKEGTEITLWAMPVQRPYNPNAKLMAEMLQSDWKKIGINAKIVSYEW
CHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHH
GEYIKRAKNGENGAMLIGWSGDNGDPDNWLGTLFGCDAVNGNNFAKWCDKPFDTLIHQAK
HHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHH
QTSDQAERTKLYKQAQHLLKDAVPMTPIAHSTVYQPMSSKVQDFKISPFGLNSFYGVSVS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHEECCCCCCCCEEEEEEC
GK
CC
>Mature Secondary Structure
MLKHAVIPFLVGASLLASAPFAHAASNLVFCSEGSPAGFDPGQYTTGTDFDASAETIFNR
CCCHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LSQFERGGTAVVPGLATKWDISEDGLTYTFHLREGVKFHTTPYFKPTREFNADDVLFTFN
HHHHHCCCCEEECCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCCCCCCCCCHHHHHHH
RMIDKDMPYRKAYPTEFPYFTDMAMDTNIKKIEKIDDHTVKFVLGMVDAAFIQNMAMSFA
HHHCCCCCCCCCCCCCCCCEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIQSAEYAAKLLKEGKPELINQQPIGTGPFVFKSYQKDSNIRYTGNKDYWKPEDVKVDNL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCEECCE
IFAITTDASVRMQKLKKNECQITAYPRPADLEPLKADKNLKMPDQAGFNLGYIAYNVMPQ
EEEEECCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEEECCC
IKGEDHPNPLAQLEVRQALDMAVNKQQIIDSVYQGAGQLAVNAMPPTQWSYDTTIKDAKY
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC
DPEKARQLLKEAGIKEGTEITLWAMPVQRPYNPNAKLMAEMLQSDWKKIGINAKIVSYEW
CHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHH
GEYIKRAKNGENGAMLIGWSGDNGDPDNWLGTLFGCDAVNGNNFAKWCDKPFDTLIHQAK
HHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHH
QTSDQAERTKLYKQAQHLLKDAVPMTPIAHSTVYQPMSSKVQDFKISPFGLNSFYGVSVS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHEECCCCCCCCEEEEEEC
GK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]