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The map label for this gene is dppA [H]

Identifier: 66047463

GI number: 66047463

Start: 5046719

End: 5048311

Strand: Direct

Name: dppA [H]

Synonym: Psyr_4236

Alternate gene names: 66047463

Gene position: 5046719-5048311 (Clockwise)

Preceding gene: 66047462

Following gene: 66047464

Centisome position: 82.82

GC content: 58.57

Gene sequence:

>1593_bases
ATGCGTAAAACTCTTGCGATGACGTCGTGCCTGGGACTGTTGGCACTGGCCCCGTTTGCCCAGGCGGCAAGCAATCTGGT
GTTTTGCTCCGAAGCGAGCCCGGCTGGCTTCGACGGTGCTCAATACACTTCCTCCACCGATAACGATGCATCCGAGCCGA
TCTACAATCGACTCACCGAATTCAAGCAAGGCGATACCACCGTGGTTCCAGCGCTGGCGACTTCCTGGGACACCTCGGAA
GACGGCCTGGTCTACACGTTTCATCTGCGCGAGGGCGTGAAGTTCCACAGCAACAAGAACTTCAAGCCCAGCCGTGACTT
CAACGCCGACGACGTGCTGTTCACCTTCAATCGCATGCTCAAGCCCGATCATCCGTTCCGTGTCGCTTACCCGACCGAGT
TCCCCTACTTCACCGGCATGGGCCTGAACAAGAAGATCAAGTCCGTCGAGAAGACCGACCCGCTGACCGTCGTCTTCACC
CTCAATACGGTCGATGCGGCATTCATCCAGAACATCGCGATGAACTTCGCCGGCATTCTCTCCGCCGAGTACGCCGAACA
GCTGATGGCCAAGGGCGACGTGCGCGACATCAATCAGCAGCCTATCGGTACAGGGCCGTTCGTGTTTCAGCGCTATCAGA
AGGATTCGCAGATTCGCTACAAGGGCAACAAGGAATTCTGGGACCCGAGCCGGGTGAAAATCGACCAGTTGATCTTCGCC
ATCGTGACCGACGCCTCCGCGCGCATGCAAAAGCTCAAGGCCAACGAATGCCAGGTCTCGGTCAACCCGCGTCCGGCGGA
CCTCGCCAGCCTCAAGGCAGACAAAAGCCTGCAGGTCATCGAGAAGCCAGGCTTCAACCTCGGCTACATCTCTTACAACG
TGCGCCACGAACCGCTGGGCAAGCTGCAAGTCCGTCAGGCGCTGGACATGGCCGTCAACAAAAAGGCGATCATCAACGCG
GTTTATCAGGGCGCGGGGCAACTGGCAGTCAACGCCATGCCACCGACCCAGTGGTCCTATGACGACAGCATCAAGGACGC
CCCCTACGACCCGGAAAAAGCCAGGACCCTGTTGCGTGCCGCTGGCGTCAAGGAAGGCACGGAACTAACCCTGTGGGCCA
TGCCGGTTCAGCGTCCGTACAACCCCAACGCCAAACTGATGGCTGAAATGCTTCAGGCCGACTGGGCCAAGGTCGGTATC
AAGGTCAAGATCGTCAGTTACGAATGGGGTGAATACCTCAAACGCACCAAAAACGGCGAGCACGATATCTCGCTGATCGG
CTGGACCGGCGACAACGGTGACCCGGACAACTGGCTGGGCACGCTTTACAGCTGCGACGCGATTGGCGGCAACAACTATT
CGATGTGGTGCGACAAGAAATACGACGACCTGATCAAGGACGCGGTTGCGACCACTGACCGCGTGAAGCGTACCGAACTG
TACCAACGGGCGCAGCGCTACCTGAAGGAGCAGGTGCCGATCACACCGATTGCCCACTCGACCGTCAATCAGCCATTGCG
CGCCGAGGTCAGGGACTTCCACGTCAGCCCGTTCGGTCGCAACAACTTTTCAGGCGTCAGCATCGTCGATTGA

Upstream 100 bases:

>100_bases
CCAGGTGTACCGGGCATGTCGTGCAAAATTGCGTGGGGCGAGGCCTTTGGGCCAGTTAGATATTAGAGCCTCGGCTCATC
CTTCGAGGATAGGGATCATC

Downstream 100 bases:

>100_bases
TCGAAAACTCCGTACGCCCTCGCCGTCTTGTGTCACGGCGAGAGCGGGTACTTGCGCGTCGATACCTATAACAACGCAGG
ACCCAAAGTCCTGCACCACC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MRKTLAMTSCLGLLALAPFAQAASNLVFCSEASPAGFDGAQYTSSTDNDASEPIYNRLTEFKQGDTTVVPALATSWDTSE
DGLVYTFHLREGVKFHSNKNFKPSRDFNADDVLFTFNRMLKPDHPFRVAYPTEFPYFTGMGLNKKIKSVEKTDPLTVVFT
LNTVDAAFIQNIAMNFAGILSAEYAEQLMAKGDVRDINQQPIGTGPFVFQRYQKDSQIRYKGNKEFWDPSRVKIDQLIFA
IVTDASARMQKLKANECQVSVNPRPADLASLKADKSLQVIEKPGFNLGYISYNVRHEPLGKLQVRQALDMAVNKKAIINA
VYQGAGQLAVNAMPPTQWSYDDSIKDAPYDPEKARTLLRAAGVKEGTELTLWAMPVQRPYNPNAKLMAEMLQADWAKVGI
KVKIVSYEWGEYLKRTKNGEHDISLIGWTGDNGDPDNWLGTLYSCDAIGGNNYSMWCDKKYDDLIKDAVATTDRVKRTEL
YQRAQRYLKEQVPITPIAHSTVNQPLRAEVRDFHVSPFGRNNFSGVSIVD

Sequences:

>Translated_530_residues
MRKTLAMTSCLGLLALAPFAQAASNLVFCSEASPAGFDGAQYTSSTDNDASEPIYNRLTEFKQGDTTVVPALATSWDTSE
DGLVYTFHLREGVKFHSNKNFKPSRDFNADDVLFTFNRMLKPDHPFRVAYPTEFPYFTGMGLNKKIKSVEKTDPLTVVFT
LNTVDAAFIQNIAMNFAGILSAEYAEQLMAKGDVRDINQQPIGTGPFVFQRYQKDSQIRYKGNKEFWDPSRVKIDQLIFA
IVTDASARMQKLKANECQVSVNPRPADLASLKADKSLQVIEKPGFNLGYISYNVRHEPLGKLQVRQALDMAVNKKAIINA
VYQGAGQLAVNAMPPTQWSYDDSIKDAPYDPEKARTLLRAAGVKEGTELTLWAMPVQRPYNPNAKLMAEMLQADWAKVGI
KVKIVSYEWGEYLKRTKNGEHDISLIGWTGDNGDPDNWLGTLYSCDAIGGNNYSMWCDKKYDDLIKDAVATTDRVKRTEL
YQRAQRYLKEQVPITPIAHSTVNQPLRAEVRDFHVSPFGRNNFSGVSIVD
>Mature_530_residues
MRKTLAMTSCLGLLALAPFAQAASNLVFCSEASPAGFDGAQYTSSTDNDASEPIYNRLTEFKQGDTTVVPALATSWDTSE
DGLVYTFHLREGVKFHSNKNFKPSRDFNADDVLFTFNRMLKPDHPFRVAYPTEFPYFTGMGLNKKIKSVEKTDPLTVVFT
LNTVDAAFIQNIAMNFAGILSAEYAEQLMAKGDVRDINQQPIGTGPFVFQRYQKDSQIRYKGNKEFWDPSRVKIDQLIFA
IVTDASARMQKLKANECQVSVNPRPADLASLKADKSLQVIEKPGFNLGYISYNVRHEPLGKLQVRQALDMAVNKKAIINA
VYQGAGQLAVNAMPPTQWSYDDSIKDAPYDPEKARTLLRAAGVKEGTELTLWAMPVQRPYNPNAKLMAEMLQADWAKVGI
KVKIVSYEWGEYLKRTKNGEHDISLIGWTGDNGDPDNWLGTLYSCDAIGGNNYSMWCDKKYDDLIKDAVATTDRVKRTEL
YQRAQRYLKEQVPITPIAHSTVNQPLRAEVRDFHVSPFGRNNFSGVSIVD

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=535, Percent_Identity=54.0186915887851, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1787551, Length=505, Percent_Identity=33.6633663366337, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1787052, Length=442, Percent_Identity=28.7330316742081, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1787762, Length=542, Percent_Identity=25.6457564575646, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789887, Length=538, Percent_Identity=24.5353159851301, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1787495, Length=527, Percent_Identity=22.011385199241, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789397, Length=472, Percent_Identity=23.728813559322, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081878, Length=558, Percent_Identity=21.8637992831541, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59374; Mature: 59374

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTLAMTSCLGLLALAPFAQAASNLVFCSEASPAGFDGAQYTSSTDNDASEPIYNRLTE
CCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
FKQGDTTVVPALATSWDTSEDGLVYTFHLREGVKFHSNKNFKPSRDFNADDVLFTFNRML
HCCCCCEEEEHEECCCCCCCCCEEEEEEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHC
KPDHPFRVAYPTEFPYFTGMGLNKKIKSVEKTDPLTVVFTLNTVDAAFIQNIAMNFAGIL
CCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHH
SAEYAEQLMAKGDVRDINQQPIGTGPFVFQRYQKDSQIRYKGNKEFWDPSRVKIDQLIFA
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHH
IVTDASARMQKLKANECQVSVNPRPADLASLKADKSLQVIEKPGFNLGYISYNVRHEPLG
HHHCCHHHHHHHCCCCCEEEECCCCCHHHHHCCCCCHHHEECCCCEEEEEEEECCCCCCH
KLQVRQALDMAVNKKAIINAVYQGAGQLAVNAMPPTQWSYDDSIKDAPYDPEKARTLLRA
HHHHHHHHHHHHCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AGVKEGTELTLWAMPVQRPYNPNAKLMAEMLQADWAKVGIKVKIVSYEWGEYLKRTKNGE
CCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECHHHHHHHCCCCC
HDISLIGWTGDNGDPDNWLGTLYSCDAIGGNNYSMWCDKKYDDLIKDAVATTDRVKRTEL
CCEEEEEEECCCCCCHHHHHHHEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
YQRAQRYLKEQVPITPIAHSTVNQPLRAEVRDFHVSPFGRNNFSGVSIVD
HHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHEECCCCCCCCCCCCEECC
>Mature Secondary Structure
MRKTLAMTSCLGLLALAPFAQAASNLVFCSEASPAGFDGAQYTSSTDNDASEPIYNRLTE
CCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
FKQGDTTVVPALATSWDTSEDGLVYTFHLREGVKFHSNKNFKPSRDFNADDVLFTFNRML
HCCCCCEEEEHEECCCCCCCCCEEEEEEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHC
KPDHPFRVAYPTEFPYFTGMGLNKKIKSVEKTDPLTVVFTLNTVDAAFIQNIAMNFAGIL
CCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHH
SAEYAEQLMAKGDVRDINQQPIGTGPFVFQRYQKDSQIRYKGNKEFWDPSRVKIDQLIFA
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHH
IVTDASARMQKLKANECQVSVNPRPADLASLKADKSLQVIEKPGFNLGYISYNVRHEPLG
HHHCCHHHHHHHCCCCCEEEECCCCCHHHHHCCCCCHHHEECCCCEEEEEEEECCCCCCH
KLQVRQALDMAVNKKAIINAVYQGAGQLAVNAMPPTQWSYDDSIKDAPYDPEKARTLLRA
HHHHHHHHHHHHCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AGVKEGTELTLWAMPVQRPYNPNAKLMAEMLQADWAKVGIKVKIVSYEWGEYLKRTKNGE
CCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECHHHHHHHCCCCC
HDISLIGWTGDNGDPDNWLGTLYSCDAIGGNNYSMWCDKKYDDLIKDAVATTDRVKRTEL
CCEEEEEEECCCCCCHHHHHHHEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
YQRAQRYLKEQVPITPIAHSTVNQPLRAEVRDFHVSPFGRNNFSGVSIVD
HHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHEECCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]