Click here to switch to the map view.

The map label for this gene is dppA [H]

Identifier: 66047461

GI number: 66047461

Start: 5042907

End: 5044514

Strand: Direct

Name: dppA [H]

Synonym: Psyr_4234

Alternate gene names: 66047461

Gene position: 5042907-5044514 (Clockwise)

Preceding gene: 66047460

Following gene: 66047462

Centisome position: 82.76

GC content: 58.89

Gene sequence:

>1608_bases
ATGGGACACACACTTTTCAAACCGCTGCTGCTGACCACCGCGCTGCTGGCCTGCGCGCCTTTGGCCCACGCGGCAAGCAC
GCTGGTGTATTGCTCTGAAGCCAGCCCGGCCGGTTTCGACCCCAGCCAGTACACCAGCGGCACCGACTTCGATGCCTCGG
CCGAAACCGTTTTCAACCGTCTGACCCAGTTCAAGCGTGGCGGTACGGAAGTGGAGCCGGGTCTGGCCACACGCTGGGAC
GTTTCGCCGGATGGTCTGACCTACACCTTTCACCTGCGCGACGGCGTGAAATTCCATACCACGGATTATTTCACCCCGAC
GCGCACGTTCAACGCCGATGACGTGCTGTTCACCTTCAACCGCCTGCTCGATGCCAACCACCCATTCCGCAAGGCGTATC
CGTCCGAGTCGCCCTACTTCACCGACATGGGCCTGAATACGACGATCAAGAGCGTCGAGAAAGTCGACCCGCTGACCGTC
AAGTTCACCCTGAACAACATCGATGCGGCCTTCGTGCAGAACCTGGCCATGAGCTTCGCATCCATTCAGTCCGCTGAATA
CGCCGACCAGTTGCTCAAGGAAGGCAAGGCCGAGGACCTGAACCAGAAGCCCATCGGCACTGGTCCGTTCGTCTTCAAGC
GCTACCAGAAGGATTCGCAGATCCGTTACGTCGGCAATACCCAGTACTGGAAGCCGGAGGATGTGAAGATCGACAACCTG
ATCTTCTCGATCAGCAGTGATGCCGCCGTGCGCCTGCAGAAGCTCAAGGCCAACGAATGCCAGGTCAGCGGCTATCCGCG
TCCACAGGACATCGAGGAGATGAAAAAGGACCCGAAACTCAAGGTCCTCAGCCAGCCAGGCTTCAACCTGGGCTTCGTGG
CCTACAACGTTACGCATCCGCCGCTGGATCAGCTCAAGGTCCGCCAGGCGCTGGACATGGCCATCGACAAGCCGGCGATC
ATCAAAGCGGTTTATCAGAGCGCGGGCCAGTTGGCTGAAAATGCGCTGCCGCCGGGTCAGTGGAGCTTCGATCCGACCAT
CAAGGACGCCCCGCACGACCTGACCAAAGCCAGGCAGCTGCTCAAGGAAGCAGGTGTAGCGCCGGGTACCAAAATCGACC
TGTGGGCCATGACTGTGCAGCGCGCGTCGAACCCCAATGCACGCATGTCGGCACAGATGATCCAGTCCGACTGGGAGAAA
ATCGGTATCAAGGCCAACATCGTCAGCTATGAATGGGGCGAATACATCAAGCGCGCCAAGGCCGGCGAGCATGACGCGAT
GATTTACGGCTGGACCGGTGATAACGGCGACCCCGACAACTGGCTCGGCGTGCTTTACAGCTGCGCAGCCGTCAAAGGCA
GCAACTACGCCAAATGGTGCGACCCGGCCTACGACAAACTGGTGCAGCAGGCCAAGCTGACCAGCAACCGCGATGAACGC
ATCAAGTTCTATCAACAGGCTCAACACATTCTCAAGCAGCAGGTGCCGATCACCCCGATTGCCAACTCCAAGGTGTTTCA
GCCGATGCGCCAGGAAGTGCAGGACTTCAAGATCAGTCCCTTCGGTCTGACACCCTTTTATGGTGTCAGCCTGGGAACAG
TGAAGTAA

Upstream 100 bases:

>100_bases
GTAAGCGTATTGTCCGACCCCGACCAAGTACGGCACTATCCGTTGTCTAGCTGATTGTCGACTTTCAAGCACGGTTACTG
CCGTACCACGCGAGGGATCC

Downstream 100 bases:

>100_bases
CAACACAGCGCCAACCGGGTGCATTTTTCTCACCCGGTTGCGCTTTATTGGTGCGCGAAATAATCGAAAAAGCGTCGCGC
AAACGATCAACTACCAAGAA

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MGHTLFKPLLLTTALLACAPLAHAASTLVYCSEASPAGFDPSQYTSGTDFDASAETVFNRLTQFKRGGTEVEPGLATRWD
VSPDGLTYTFHLRDGVKFHTTDYFTPTRTFNADDVLFTFNRLLDANHPFRKAYPSESPYFTDMGLNTTIKSVEKVDPLTV
KFTLNNIDAAFVQNLAMSFASIQSAEYADQLLKEGKAEDLNQKPIGTGPFVFKRYQKDSQIRYVGNTQYWKPEDVKIDNL
IFSISSDAAVRLQKLKANECQVSGYPRPQDIEEMKKDPKLKVLSQPGFNLGFVAYNVTHPPLDQLKVRQALDMAIDKPAI
IKAVYQSAGQLAENALPPGQWSFDPTIKDAPHDLTKARQLLKEAGVAPGTKIDLWAMTVQRASNPNARMSAQMIQSDWEK
IGIKANIVSYEWGEYIKRAKAGEHDAMIYGWTGDNGDPDNWLGVLYSCAAVKGSNYAKWCDPAYDKLVQQAKLTSNRDER
IKFYQQAQHILKQQVPITPIANSKVFQPMRQEVQDFKISPFGLTPFYGVSLGTVK

Sequences:

>Translated_535_residues
MGHTLFKPLLLTTALLACAPLAHAASTLVYCSEASPAGFDPSQYTSGTDFDASAETVFNRLTQFKRGGTEVEPGLATRWD
VSPDGLTYTFHLRDGVKFHTTDYFTPTRTFNADDVLFTFNRLLDANHPFRKAYPSESPYFTDMGLNTTIKSVEKVDPLTV
KFTLNNIDAAFVQNLAMSFASIQSAEYADQLLKEGKAEDLNQKPIGTGPFVFKRYQKDSQIRYVGNTQYWKPEDVKIDNL
IFSISSDAAVRLQKLKANECQVSGYPRPQDIEEMKKDPKLKVLSQPGFNLGFVAYNVTHPPLDQLKVRQALDMAIDKPAI
IKAVYQSAGQLAENALPPGQWSFDPTIKDAPHDLTKARQLLKEAGVAPGTKIDLWAMTVQRASNPNARMSAQMIQSDWEK
IGIKANIVSYEWGEYIKRAKAGEHDAMIYGWTGDNGDPDNWLGVLYSCAAVKGSNYAKWCDPAYDKLVQQAKLTSNRDER
IKFYQQAQHILKQQVPITPIANSKVFQPMRQEVQDFKISPFGLTPFYGVSLGTVK
>Mature_534_residues
GHTLFKPLLLTTALLACAPLAHAASTLVYCSEASPAGFDPSQYTSGTDFDASAETVFNRLTQFKRGGTEVEPGLATRWDV
SPDGLTYTFHLRDGVKFHTTDYFTPTRTFNADDVLFTFNRLLDANHPFRKAYPSESPYFTDMGLNTTIKSVEKVDPLTVK
FTLNNIDAAFVQNLAMSFASIQSAEYADQLLKEGKAEDLNQKPIGTGPFVFKRYQKDSQIRYVGNTQYWKPEDVKIDNLI
FSISSDAAVRLQKLKANECQVSGYPRPQDIEEMKKDPKLKVLSQPGFNLGFVAYNVTHPPLDQLKVRQALDMAIDKPAII
KAVYQSAGQLAENALPPGQWSFDPTIKDAPHDLTKARQLLKEAGVAPGTKIDLWAMTVQRASNPNARMSAQMIQSDWEKI
GIKANIVSYEWGEYIKRAKAGEHDAMIYGWTGDNGDPDNWLGVLYSCAAVKGSNYAKWCDPAYDKLVQQAKLTSNRDERI
KFYQQAQHILKQQVPITPIANSKVFQPMRQEVQDFKISPFGLTPFYGVSLGTVK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=527, Percent_Identity=52.1821631878558, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI1787551, Length=505, Percent_Identity=36.2376237623762, Blast_Score=337, Evalue=9e-94,
Organism=Escherichia coli, GI1787052, Length=522, Percent_Identity=28.1609195402299, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1787762, Length=535, Percent_Identity=27.8504672897196, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87081878, Length=501, Percent_Identity=26.1477045908184, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789397, Length=536, Percent_Identity=21.8283582089552, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789887, Length=530, Percent_Identity=23.5849056603774, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1787495, Length=462, Percent_Identity=21.2121212121212, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59760; Mature: 59629

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHTLFKPLLLTTALLACAPLAHAASTLVYCSEASPAGFDPSQYTSGTDFDASAETVFNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHH
LTQFKRGGTEVEPGLATRWDVSPDGLTYTFHLRDGVKFHTTDYFTPTRTFNADDVLFTFN
HHHHHCCCCCCCCCCCEECCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHH
RLLDANHPFRKAYPSESPYFTDMGLNTTIKSVEKVDPLTVKFTLNNIDAAFVQNLAMSFA
HHHCCCCCHHHCCCCCCCEEEECCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHH
SIQSAEYADQLLKEGKAEDLNQKPIGTGPFVFKRYQKDSQIRYVGNTQYWKPEDVKIDNL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCEEEEE
IFSISSDAAVRLQKLKANECQVSGYPRPQDIEEMKKDPKLKVLSQPGFNLGFVAYNVTHP
EEEECCCHHHHHHHHCCCCCEECCCCCCCCHHHHHCCCCEEEECCCCCCEEEEEEECCCC
PLDQLKVRQALDMAIDKPAIIKAVYQSAGQLAENALPPGQWSFDPTIKDAPHDLTKARQL
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
LKEAGVAPGTKIDLWAMTVQRASNPNARMSAQMIQSDWEKIGIKANIVSYEWGEYIKRAK
HHHCCCCCCCEEEHEEHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHC
AGEHDAMIYGWTGDNGDPDNWLGVLYSCAAVKGSNYAKWCDPAYDKLVQQAKLTSNRDER
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
IKFYQQAQHILKQQVPITPIANSKVFQPMRQEVQDFKISPFGLTPFYGVSLGTVK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GHTLFKPLLLTTALLACAPLAHAASTLVYCSEASPAGFDPSQYTSGTDFDASAETVFNR
CCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHH
LTQFKRGGTEVEPGLATRWDVSPDGLTYTFHLRDGVKFHTTDYFTPTRTFNADDVLFTFN
HHHHHCCCCCCCCCCCEECCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHH
RLLDANHPFRKAYPSESPYFTDMGLNTTIKSVEKVDPLTVKFTLNNIDAAFVQNLAMSFA
HHHCCCCCHHHCCCCCCCEEEECCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHH
SIQSAEYADQLLKEGKAEDLNQKPIGTGPFVFKRYQKDSQIRYVGNTQYWKPEDVKIDNL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCEEEEE
IFSISSDAAVRLQKLKANECQVSGYPRPQDIEEMKKDPKLKVLSQPGFNLGFVAYNVTHP
EEEECCCHHHHHHHHCCCCCEECCCCCCCCHHHHHCCCCEEEECCCCCCEEEEEEECCCC
PLDQLKVRQALDMAIDKPAIIKAVYQSAGQLAENALPPGQWSFDPTIKDAPHDLTKARQL
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
LKEAGVAPGTKIDLWAMTVQRASNPNARMSAQMIQSDWEKIGIKANIVSYEWGEYIKRAK
HHHCCCCCCCEEEHEEHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHC
AGEHDAMIYGWTGDNGDPDNWLGVLYSCAAVKGSNYAKWCDPAYDKLVQQAKLTSNRDER
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
IKFYQQAQHILKQQVPITPIANSKVFQPMRQEVQDFKISPFGLTPFYGVSLGTVK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]