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The map label for this gene is murE [H]

Identifier: 66047334

GI number: 66047334

Start: 4896998

End: 4898461

Strand: Reverse

Name: murE [H]

Synonym: Psyr_4107

Alternate gene names: 66047334

Gene position: 4898461-4896998 (Counterclockwise)

Preceding gene: 66047335

Following gene: 66047333

Centisome position: 80.39

GC content: 62.36

Gene sequence:

>1464_bases
ATGGCCTTTAATCTGAGCAAGATTTTCGCTCACACCGACCGCGATGCGCTGATTCGCGAGTTGACCCTCGACAGCCGCAA
TGTGCGCCCGGGCGACCTGTTTCTGGCAGTGCCGGGCATCAAGGTCGATGGCCGCGCGCATATCGCCGACGCGCTCAAGC
GTGGCGCAGCTGCTGTAGCCTATGAAGTCGCAGGGTCGACCGTGCTGCCGATCACCGACGTGCCGCTGATCCCGGTCAAG
GGGCTCGCCGGGCAGTTGTCCGCCATCGCCGGTCGCTTCTATGGCGATCCGAGCCGCAGTCTGAATCTGGTCGGTGTTAC
CGGCACCAACGGCAAGACCAGCGTGACCCAACTGGTGGCCCAGGCGCTTGATGTCCTCGGCCAACGTTGCGGGATCGTCG
GTACCTTGGGCACCGGTTTCTACGGCTCGCTGCAAAGCGGTCGCCACACCACGCCCGACCCGATTGCCGTGCAGGCGACG
CTGACCGACCTGAGAAAGGCCGGTGCGCGCGCAGTGGCGATGGAAGTCTCGTCCCACGGTCTTGATCAGGGACGCGCTAC
GGCGCTGGCCTTTGATGTGGCGGTGCTGACTAATCTGTCCCGTGACCATCTGGATTATCACGGCACCATGGAGGCCTACG
CTGCCGCCAAGGCGAAGCTGTTCGCATGGTCCGATCTGAAATGCCGGGTGATCAACCTGGATGACGACTTCGGTCGTGAA
CTGGCCGCGGTCAAACAAGAGTCGCGGTTGATCACCTACAGCCAGCTGGACAGTTCGGCCTACCTGTATTGCCGCGATGC
GAAGTTCGATGACGATGGCGTGCGTGCCACGCTGGTCACGCCGCAGGGCGAGCATTTCCTGCGCAGCAGCCTGCTGGGTC
GTTTCAATCTGAGCAATGTGCTGGCTGCGGTCGGCGCGCTGCTGGGCCTGGATTACGCGCTGGACGAAATTCTCAAGGCG
CTGCCGAAACTCGAAGGCCCGGTCGGACGCATGCAGCGTCTGGGTGGTGCCGACAAGCCTCTGGTGGTCGTGGATTACGC
GCACACCCCGGACGCGCTGGAAAAAGTACTCGAAGCACTGCGCCCGCACGCCAAGGGCCGCTTGTTGTGCCTGTTCGGCT
GCGGCGGTGATCGTGACCGTGGCAAGCGTCCGTTGATGGCTGGAGTGGTCGAGCGTCTGGCCGACGGCGTATGGGTCACT
GATGACAATCCGCGCTCCGAATCGCCTGTCAGTATTTTTGACGATATACGCCCTGGCTTCGTTGCTGCGGACAAGGTGCG
TTTCATCGAAGGTCGCGGTCAGGCCATTGCCGAGCTGATTGCCAGCGCCAATGCTGATGACGTGGTGGTGCTTGCTGGCA
AGGGCCACGAGGACTACCAGGAAATCAACGGTCAGCGGCAGCCGTTCTCCGATTTGCAAGAAGCCGCCAGTGCGCTTGCC
GTATGGGAGCCTGCGAATGCTTAA

Upstream 100 bases:

>100_bases
TCATGTCCGGCACGTTGCGTCTGATGAACGTGACGCCGGACAACCTTGCGCCACCTGAACAAGTCAACGCCGCACCGGCA
GCCAAAGGAGGGCGTGGTTG

Downstream 100 bases:

>100_bases
GCCAATGACCTTCCGCGAACTGGTTGTACCACTCGACGCCCGTCTGGTCGGCGGCGACTGCAGCTTTGCCGGCGTGAGCA
CCGACAGTCGTGGCATCGAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MAFNLSKIFAHTDRDALIRELTLDSRNVRPGDLFLAVPGIKVDGRAHIADALKRGAAAVAYEVAGSTVLPITDVPLIPVK
GLAGQLSAIAGRFYGDPSRSLNLVGVTGTNGKTSVTQLVAQALDVLGQRCGIVGTLGTGFYGSLQSGRHTTPDPIAVQAT
LTDLRKAGARAVAMEVSSHGLDQGRATALAFDVAVLTNLSRDHLDYHGTMEAYAAAKAKLFAWSDLKCRVINLDDDFGRE
LAAVKQESRLITYSQLDSSAYLYCRDAKFDDDGVRATLVTPQGEHFLRSSLLGRFNLSNVLAAVGALLGLDYALDEILKA
LPKLEGPVGRMQRLGGADKPLVVVDYAHTPDALEKVLEALRPHAKGRLLCLFGCGGDRDRGKRPLMAGVVERLADGVWVT
DDNPRSESPVSIFDDIRPGFVAADKVRFIEGRGQAIAELIASANADDVVVLAGKGHEDYQEINGQRQPFSDLQEAASALA
VWEPANA

Sequences:

>Translated_487_residues
MAFNLSKIFAHTDRDALIRELTLDSRNVRPGDLFLAVPGIKVDGRAHIADALKRGAAAVAYEVAGSTVLPITDVPLIPVK
GLAGQLSAIAGRFYGDPSRSLNLVGVTGTNGKTSVTQLVAQALDVLGQRCGIVGTLGTGFYGSLQSGRHTTPDPIAVQAT
LTDLRKAGARAVAMEVSSHGLDQGRATALAFDVAVLTNLSRDHLDYHGTMEAYAAAKAKLFAWSDLKCRVINLDDDFGRE
LAAVKQESRLITYSQLDSSAYLYCRDAKFDDDGVRATLVTPQGEHFLRSSLLGRFNLSNVLAAVGALLGLDYALDEILKA
LPKLEGPVGRMQRLGGADKPLVVVDYAHTPDALEKVLEALRPHAKGRLLCLFGCGGDRDRGKRPLMAGVVERLADGVWVT
DDNPRSESPVSIFDDIRPGFVAADKVRFIEGRGQAIAELIASANADDVVVLAGKGHEDYQEINGQRQPFSDLQEAASALA
VWEPANA
>Mature_486_residues
AFNLSKIFAHTDRDALIRELTLDSRNVRPGDLFLAVPGIKVDGRAHIADALKRGAAAVAYEVAGSTVLPITDVPLIPVKG
LAGQLSAIAGRFYGDPSRSLNLVGVTGTNGKTSVTQLVAQALDVLGQRCGIVGTLGTGFYGSLQSGRHTTPDPIAVQATL
TDLRKAGARAVAMEVSSHGLDQGRATALAFDVAVLTNLSRDHLDYHGTMEAYAAAKAKLFAWSDLKCRVINLDDDFGREL
AAVKQESRLITYSQLDSSAYLYCRDAKFDDDGVRATLVTPQGEHFLRSSLLGRFNLSNVLAAVGALLGLDYALDEILKAL
PKLEGPVGRMQRLGGADKPLVVVDYAHTPDALEKVLEALRPHAKGRLLCLFGCGGDRDRGKRPLMAGVVERLADGVWVTD
DNPRSESPVSIFDDIRPGFVAADKVRFIEGRGQAIAELIASANADDVVVLAGKGHEDYQEINGQRQPFSDLQEAASALAV
WEPANA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=472, Percent_Identity=45.1271186440678, Blast_Score=348, Evalue=4e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51989; Mature: 51857

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNLSKIFAHTDRDALIRELTLDSRNVRPGDLFLAVPGIKVDGRAHIADALKRGAAAVA
CCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEECCEEECCCHHHHHHHHCCCCEEE
YEVAGSTVLPITDVPLIPVKGLAGQLSAIAGRFYGDPSRSLNLVGVTGTNGKTSVTQLVA
EEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHH
QALDVLGQRCGIVGTLGTGFYGSLQSGRHTTPDPIAVQATLTDLRKAGARAVAMEVSSHG
HHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEHHHCC
LDQGRATALAFDVAVLTNLSRDHLDYHGTMEAYAAAKAKLFAWSDLKCRVINLDDDFGRE
CCCCCHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHEEEEECCCEEEEEECCHHHHHH
LAAVKQESRLITYSQLDSSAYLYCRDAKFDDDGVRATLVTPQGEHFLRSSLLGRFNLSNV
HHHHHHHCCEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHH
LAAVGALLGLDYALDEILKALPKLEGPVGRMQRLGGADKPLVVVDYAHTPDALEKVLEAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
RPHAKGRLLCLFGCGGDRDRGKRPLMAGVVERLADGVWVTDDNPRSESPVSIFDDIRPGF
CCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHCCCCCC
VAADKVRFIEGRGQAIAELIASANADDVVVLAGKGHEDYQEINGQRQPFSDLQEAASALA
EECCCEEEECCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHEE
VWEPANA
ECCCCCC
>Mature Secondary Structure 
AFNLSKIFAHTDRDALIRELTLDSRNVRPGDLFLAVPGIKVDGRAHIADALKRGAAAVA
CCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEECCEEECCCHHHHHHHHCCCCEEE
YEVAGSTVLPITDVPLIPVKGLAGQLSAIAGRFYGDPSRSLNLVGVTGTNGKTSVTQLVA
EEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHH
QALDVLGQRCGIVGTLGTGFYGSLQSGRHTTPDPIAVQATLTDLRKAGARAVAMEVSSHG
HHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEHHHCC
LDQGRATALAFDVAVLTNLSRDHLDYHGTMEAYAAAKAKLFAWSDLKCRVINLDDDFGRE
CCCCCHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHEEEEECCCEEEEEECCHHHHHH
LAAVKQESRLITYSQLDSSAYLYCRDAKFDDDGVRATLVTPQGEHFLRSSLLGRFNLSNV
HHHHHHHCCEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHH
LAAVGALLGLDYALDEILKALPKLEGPVGRMQRLGGADKPLVVVDYAHTPDALEKVLEAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
RPHAKGRLLCLFGCGGDRDRGKRPLMAGVVERLADGVWVTDDNPRSESPVSIFDDIRPGF
CCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHCCCCCC
VAADKVRFIEGRGQAIAELIASANADDVVVLAGKGHEDYQEINGQRQPFSDLQEAASALA
EECCCEEEECCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHEE
VWEPANA
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]