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The map label for this gene is murF [H]

Identifier: 66047333

GI number: 66047333

Start: 4895635

End: 4897005

Strand: Reverse

Name: murF [H]

Synonym: Psyr_4106

Alternate gene names: 66047333

Gene position: 4897005-4895635 (Counterclockwise)

Preceding gene: 66047334

Following gene: 66047332

Centisome position: 80.36

GC content: 63.53

Gene sequence:

>1371_bases
ATGCTTAAGCCAATGACCTTCCGCGAACTGGTTGTACCACTCGACGCCCGTCTGGTCGGCGGCGACTGCAGCTTTGCCGG
CGTGAGCACCGACAGTCGTGGCATCGAGCCTGGGCAACTGTTCGTGGCCCTGACCGGCCCGCGTTTCGACGGTCATGAAT
ACCTCGACCAAGTGGCTGCCAAAGGTGCGGTCGGTGCGCTGGTCGAGCGCGAAGTGCAGGGCGCCGCCTTGCCGCAACTG
GTCGTGCTGGATACCCGCAAGGCACTTGCCCAGCTGGGTGCCATGAATCGCAATGCGTTCGTCGACCGGCCGGTTGCTGC
CGTCACCGGGTCGAGTGGCAAAACCACGGTCAAGGAGATGCTCGCGAGCATCCTGCGCACGCGCGGGCCTGTGCTGGCGA
CCCGAGGCAACCTGAACAACGAATTGGGCGTTCCACTGACGCTGCTGGAACTGGCTCCCGAATACACGTCCGCTGTGATC
GAACTGGGTGCATCGCGGGTTGGCGAGATTGCCTACACCGTGAGCCTTGCCAAGCCGCACGTCGCCATGATTACCAACGC
CGGCACCGCCCATGTTGGCGAGTTTGGCGGCCCGGACAGGATTGTCGAAGCCAAGGGCGAGATTCTGGAAGGTCTCGACG
CGGGTGGCACTGCAGTCTTGAACCTGGAAGACAAGGCGTTTCCGATCTGGCGCAAGCGCGCCGGCAATCGCAATATCCTG
ACCTTCGCACTGACCGACTCTGCAGCGGATGTGTACGCCAGCGAGCTCAGCCGTGATGCTCGGGGCTGCCCGGCTTTCAA
CCTCAACAGCCCGCTCGGCACCGCCCGGGTTCAGCTGAACCTGCTCGGCACGCACAATGTGTCCAACGCCCTGGCCGCTG
CGGCTGCCGGCTGCGCAATGGGTGTGCCGCTGGAAGGGATCGTTACCGGGCTGAACAACGTGCAGCCGGTCAAGGGCCGT
GCCGTGGCGCAAATCGCCAGCAATGGCACGCGCGTCATTGATGATACTTACAACGCCAACCCTGCCTCGATCAACGCGGC
CGTCGACATCCTCACCGGCTTCACCGGTCGTACCGTACTGGTGCTGGGCGATATCGGCGAGCTGGGCGACTGGGCCGAGC
AGGGCCATCGCGAAGTCGGTGCCTATGCCGCTGGCAAGGTGTCGGCGCTGTATGCCGTCGGCCCGTTGATGACTCACGCC
GTCAGCGCCTTTGGCGAGCACGCTTACCACTTTGCCAGTCAGGCTGAACTGATTGCGGCGCTGGGCGCCGAGCAGGACCC
CGATACCACTTTACTGATCAAGGGCTCGCGTAGCGCTGCGATGGAACACGTCGTGGCTGCCTTGTGCGGTTCTAGCCTGG
AGAAACATTAA

Upstream 100 bases:

>100_bases
GGCAAGGGCCACGAGGACTACCAGGAAATCAACGGTCAGCGGCAGCCGTTCTCCGATTTGCAAGAAGCCGCCAGTGCGCT
TGCCGTATGGGAGCCTGCGA

Downstream 100 bases:

>100_bases
TGCTGCTGCTGTTGGCCGAGTTTTTACAACAGTTCTACAAAGGCTTCGCGGTCTTTCAGTACCTGTCATTGCGCGGGATT
CTTGGCGTGCTCACTGCACT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLKPMTFRELVVPLDARLVGGDCSFAGVSTDSRGIEPGQLFVALTGPRFDGHEYLDQVAAKGAVGALVEREVQGAALPQL
VVLDTRKALAQLGAMNRNAFVDRPVAAVTGSSGKTTVKEMLASILRTRGPVLATRGNLNNELGVPLTLLELAPEYTSAVI
ELGASRVGEIAYTVSLAKPHVAMITNAGTAHVGEFGGPDRIVEAKGEILEGLDAGGTAVLNLEDKAFPIWRKRAGNRNIL
TFALTDSAADVYASELSRDARGCPAFNLNSPLGTARVQLNLLGTHNVSNALAAAAAGCAMGVPLEGIVTGLNNVQPVKGR
AVAQIASNGTRVIDDTYNANPASINAAVDILTGFTGRTVLVLGDIGELGDWAEQGHREVGAYAAGKVSALYAVGPLMTHA
VSAFGEHAYHFASQAELIAALGAEQDPDTTLLIKGSRSAAMEHVVAALCGSSLEKH

Sequences:

>Translated_456_residues
MLKPMTFRELVVPLDARLVGGDCSFAGVSTDSRGIEPGQLFVALTGPRFDGHEYLDQVAAKGAVGALVEREVQGAALPQL
VVLDTRKALAQLGAMNRNAFVDRPVAAVTGSSGKTTVKEMLASILRTRGPVLATRGNLNNELGVPLTLLELAPEYTSAVI
ELGASRVGEIAYTVSLAKPHVAMITNAGTAHVGEFGGPDRIVEAKGEILEGLDAGGTAVLNLEDKAFPIWRKRAGNRNIL
TFALTDSAADVYASELSRDARGCPAFNLNSPLGTARVQLNLLGTHNVSNALAAAAAGCAMGVPLEGIVTGLNNVQPVKGR
AVAQIASNGTRVIDDTYNANPASINAAVDILTGFTGRTVLVLGDIGELGDWAEQGHREVGAYAAGKVSALYAVGPLMTHA
VSAFGEHAYHFASQAELIAALGAEQDPDTTLLIKGSRSAAMEHVVAALCGSSLEKH
>Mature_456_residues
MLKPMTFRELVVPLDARLVGGDCSFAGVSTDSRGIEPGQLFVALTGPRFDGHEYLDQVAAKGAVGALVEREVQGAALPQL
VVLDTRKALAQLGAMNRNAFVDRPVAAVTGSSGKTTVKEMLASILRTRGPVLATRGNLNNELGVPLTLLELAPEYTSAVI
ELGASRVGEIAYTVSLAKPHVAMITNAGTAHVGEFGGPDRIVEAKGEILEGLDAGGTAVLNLEDKAFPIWRKRAGNRNIL
TFALTDSAADVYASELSRDARGCPAFNLNSPLGTARVQLNLLGTHNVSNALAAAAAGCAMGVPLEGIVTGLNNVQPVKGR
AVAQIASNGTRVIDDTYNANPASINAAVDILTGFTGRTVLVLGDIGELGDWAEQGHREVGAYAAGKVSALYAVGPLMTHA
VSAFGEHAYHFASQAELIAALGAEQDPDTTLLIKGSRSAAMEHVVAALCGSSLEKH

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=450, Percent_Identity=44.8888888888889, Blast_Score=297, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 47298; Mature: 47298

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPMTFRELVVPLDARLVGGDCSFAGVSTDSRGIEPGQLFVALTGPRFDGHEYLDQVAA
CCCCCCHHHHHCCCCCEEECCCCCEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
KGAVGALVEREVQGAALPQLVVLDTRKALAQLGAMNRNAFVDRPVAAVTGSSGKTTVKEM
CCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHH
LASILRTRGPVLATRGNLNNELGVPLTLLELAPEYTSAVIELGASRVGEIAYTVSLAKPH
HHHHHHCCCCEEEECCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCCC
VAMITNAGTAHVGEFGGPDRIVEAKGEILEGLDAGGTAVLNLEDKAFPIWRKRAGNRNIL
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCEE
TFALTDSAADVYASELSRDARGCPAFNLNSPLGTARVQLNLLGTHNVSNALAAAAAGCAM
EEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHC
GVPLEGIVTGLNNVQPVKGRAVAQIASNGTRVIDDTYNANPASINAAVDILTGFTGRTVL
CCCHHHHHHCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCEEE
VLGDIGELGDWAEQGHREVGAYAAGKVSALYAVGPLMTHAVSAFGEHAYHFASQAELIAA
EEECCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAEQDPDTTLLIKGSRSAAMEHVVAALCGSSLEKH
HCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKPMTFRELVVPLDARLVGGDCSFAGVSTDSRGIEPGQLFVALTGPRFDGHEYLDQVAA
CCCCCCHHHHHCCCCCEEECCCCCEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
KGAVGALVEREVQGAALPQLVVLDTRKALAQLGAMNRNAFVDRPVAAVTGSSGKTTVKEM
CCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHH
LASILRTRGPVLATRGNLNNELGVPLTLLELAPEYTSAVIELGASRVGEIAYTVSLAKPH
HHHHHHCCCCEEEECCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCCC
VAMITNAGTAHVGEFGGPDRIVEAKGEILEGLDAGGTAVLNLEDKAFPIWRKRAGNRNIL
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCEE
TFALTDSAADVYASELSRDARGCPAFNLNSPLGTARVQLNLLGTHNVSNALAAAAAGCAM
EEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHC
GVPLEGIVTGLNNVQPVKGRAVAQIASNGTRVIDDTYNANPASINAAVDILTGFTGRTVL
CCCHHHHHHCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCEEE
VLGDIGELGDWAEQGHREVGAYAAGKVSALYAVGPLMTHAVSAFGEHAYHFASQAELIAA
EEECCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAEQDPDTTLLIKGSRSAAMEHVVAALCGSSLEKH
HCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]