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The map label for this gene is pcaF [H]

Identifier: 66047238

GI number: 66047238

Start: 4778415

End: 4779614

Strand: Reverse

Name: pcaF [H]

Synonym: Psyr_4011

Alternate gene names: 66047238

Gene position: 4779614-4778415 (Counterclockwise)

Preceding gene: 66047239

Following gene: 66047237

Centisome position: 78.44

GC content: 64.33

Gene sequence:

>1200_bases
ATGCGTGAAGTGTTTATTTGCGATGCGATTCGTACGCCCATCGGTCGCTTTGGTGGCGGGCTGTCTTCGGTGCGTGCAGA
CGACCTGGCGGCGGTGCCGATCAAGGCGCTGATGGAGCGCAACCCGTCGGTCAACTGGGAGGAAATCGACGAAGTGTTCT
TCGGCTGTGCCAATCAGGCCGGTGAGGACAACCGCAACGTTGCGCGCATGGCCGCACTGTTGGCCGGGCTGCCACAAAGC
GTACCGGGGGTCACCCTCAATCGCCTGTGTGCGTCCGGGATGGACGCCATCGGCACCGCTTTCCGGGCCATCGCTTCAGG
TGAAATCGAGCTGGCGATCGCCGGAGGCGTCGAGTCGATGTCCCGCGCGCCCTTCGTGATGGGCAAGGCCGATGCCGCGT
TTTCGCGCAACATGAAGCTGGAAGACACCACCATCGGCTGGCGCTTTATCAATCCGGCGATGAAAGCGCAGTACGGCGTG
GACTCGATGCCCGAGACGGGCGACAACGTTGCAACCGACTTCAGGATTTCTCGAGCCGATCAGGACGCCTTCGCCCTGCG
CAGCCAGCAACGCACTGCCGTTGCCCAGGCTGCGGGCTTTTTCGAGGAAGAGATCGTGCCGGTGCGAGTCGCGCACAAAA
AGGGCGAGACCCTTGTCGACAAGGATGAGCATCCGCGTGGCGATACCAGTCTGGAAACCCTGAGCAAACTCAAGCCGGTC
AACGGCCCGGACCGCACGGTGACTGCCGGTAACGCGTCCGGGGTCAACGACGGTGCTGCTGCGCTGATTCTGGCGTCGGC
CGAAGCGGTGAAAAAGCATGGCCTGACGCCGCGTGCCCGTGTGCTCGGCATGGCCAGTGCGGCGGTTGCACCTCGGGTGA
TGGGCATTGGTCCGGTGCCAGCGGTGCGTAAGCTGGTCGAGCGGCTGGGCCTGGCGGTGACCGATTTCGATGTCATCGAG
CTCAATGAAGCATTTGCCAGCCAGGGGCTTGCGGTCTTGCGCGAACTGGGTCTGGCCGACGATGCACCGCAGGTCAATCC
CAACGGAGGGGCGATTGCCCTCGGCCACCCGCTGGGCATGAGCGGCGCGCGGCTGGTGCTGACGGCGCTGCATCATCTGG
AGAAAACCGGCGGGCGCAGAGGCCTGGCAACCATGTGCGTCGGCGTCGGGCAGGGGCTCGCACTGGCCATCGAGCGGTAA

Upstream 100 bases:

>100_bases
TTGTTGTGTCCGAAGAGCCTACCGAGGTCGAGCTGACGGCGCTGCGTGATCTGGAAGCACGGACTGCCGCGGCTCACGGT
CAGGTTGCAGGAGAAGCGTG

Downstream 100 bases:

>100_bases
ACCCTCCTGCTTCAACCTGTTGTCCAGCAACCCGGATCGTTTGTCTGCCTCCACGAAAAGGCGGGCCGATGATCCGGGTT
GTTGTTCGTCCGGGCGCGGA

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MREVFICDAIRTPIGRFGGGLSSVRADDLAAVPIKALMERNPSVNWEEIDEVFFGCANQAGEDNRNVARMAALLAGLPQS
VPGVTLNRLCASGMDAIGTAFRAIASGEIELAIAGGVESMSRAPFVMGKADAAFSRNMKLEDTTIGWRFINPAMKAQYGV
DSMPETGDNVATDFRISRADQDAFALRSQQRTAVAQAAGFFEEEIVPVRVAHKKGETLVDKDEHPRGDTSLETLSKLKPV
NGPDRTVTAGNASGVNDGAAALILASAEAVKKHGLTPRARVLGMASAAVAPRVMGIGPVPAVRKLVERLGLAVTDFDVIE
LNEAFASQGLAVLRELGLADDAPQVNPNGGAIALGHPLGMSGARLVLTALHHLEKTGGRRGLATMCVGVGQGLALAIER

Sequences:

>Translated_399_residues
MREVFICDAIRTPIGRFGGGLSSVRADDLAAVPIKALMERNPSVNWEEIDEVFFGCANQAGEDNRNVARMAALLAGLPQS
VPGVTLNRLCASGMDAIGTAFRAIASGEIELAIAGGVESMSRAPFVMGKADAAFSRNMKLEDTTIGWRFINPAMKAQYGV
DSMPETGDNVATDFRISRADQDAFALRSQQRTAVAQAAGFFEEEIVPVRVAHKKGETLVDKDEHPRGDTSLETLSKLKPV
NGPDRTVTAGNASGVNDGAAALILASAEAVKKHGLTPRARVLGMASAAVAPRVMGIGPVPAVRKLVERLGLAVTDFDVIE
LNEAFASQGLAVLRELGLADDAPQVNPNGGAIALGHPLGMSGARLVLTALHHLEKTGGRRGLATMCVGVGQGLALAIER
>Mature_399_residues
MREVFICDAIRTPIGRFGGGLSSVRADDLAAVPIKALMERNPSVNWEEIDEVFFGCANQAGEDNRNVARMAALLAGLPQS
VPGVTLNRLCASGMDAIGTAFRAIASGEIELAIAGGVESMSRAPFVMGKADAAFSRNMKLEDTTIGWRFINPAMKAQYGV
DSMPETGDNVATDFRISRADQDAFALRSQQRTAVAQAAGFFEEEIVPVRVAHKKGETLVDKDEHPRGDTSLETLSKLKPV
NGPDRTVTAGNASGVNDGAAALILASAEAVKKHGLTPRARVLGMASAAVAPRVMGIGPVPAVRKLVERLGLAVTDFDVIE
LNEAFASQGLAVLRELGLADDAPQVNPNGGAIALGHPLGMSGARLVLTALHHLEKTGGRRGLATMCVGVGQGLALAIER

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=400, Percent_Identity=43, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=41.9597989949749, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI4557237, Length=401, Percent_Identity=39.1521197007481, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI4501853, Length=403, Percent_Identity=38.9578163771712, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=33.0210772833724, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI194353979, Length=399, Percent_Identity=29.5739348370927, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=67, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1788554, Length=401, Percent_Identity=44.3890274314214, Blast_Score=294, Evalue=8e-81,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=44.3890274314214, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=41.7283950617284, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1788683, Length=419, Percent_Identity=31.5035799522673, Blast_Score=155, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=40.7035175879397, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=39.8514851485149, Blast_Score=254, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=35.4679802955665, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17535917, Length=412, Percent_Identity=34.4660194174757, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17551802, Length=427, Percent_Identity=32.3185011709602, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6322031, Length=399, Percent_Identity=37.0927318295739, Blast_Score=222, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6325229, Length=405, Percent_Identity=37.2839506172839, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24655093, Length=399, Percent_Identity=45.1127819548872, Blast_Score=334, Evalue=6e-92,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=37.468982630273, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24640423, Length=398, Percent_Identity=35.427135678392, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=32.5526932084309, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41836; Mature: 41836

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREVFICDAIRTPIGRFGGGLSSVRADDLAAVPIKALMERNPSVNWEEIDEVFFGCANQA
CCCEEEEHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMAALLAGLPQSVPGVTLNRLCASGMDAIGTAFRAIASGEIELAIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCHHHH
SRAPFVMGKADAAFSRNMKLEDTTIGWRFINPAMKAQYGVDSMPETGDNVATDFRISRAD
HCCCEEEECCCHHHHCCCCCCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCEEEECCC
QDAFALRSQQRTAVAQAAGFFEEEIVPVRVAHKKGETLVDKDEHPRGDTSLETLSKLKPV
CHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCC
NGPDRTVTAGNASGVNDGAAALILASAEAVKKHGLTPRARVLGMASAAVAPRVMGIGPVP
CCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCHHCCCCCCH
AVRKLVERLGLAVTDFDVIELNEAFASQGLAVLRELGLADDAPQVNPNGGAIALGHPLGM
HHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
SGARLVLTALHHLEKTGGRRGLATMCVGVGQGLALAIER
CHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MREVFICDAIRTPIGRFGGGLSSVRADDLAAVPIKALMERNPSVNWEEIDEVFFGCANQA
CCCEEEEHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMAALLAGLPQSVPGVTLNRLCASGMDAIGTAFRAIASGEIELAIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCHHHH
SRAPFVMGKADAAFSRNMKLEDTTIGWRFINPAMKAQYGVDSMPETGDNVATDFRISRAD
HCCCEEEECCCHHHHCCCCCCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCEEEECCC
QDAFALRSQQRTAVAQAAGFFEEEIVPVRVAHKKGETLVDKDEHPRGDTSLETLSKLKPV
CHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCC
NGPDRTVTAGNASGVNDGAAALILASAEAVKKHGLTPRARVLGMASAAVAPRVMGIGPVP
CCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCHHCCCCCCH
AVRKLVERLGLAVTDFDVIELNEAFASQGLAVLRELGLADDAPQVNPNGGAIALGHPLGM
HHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
SGARLVLTALHHLEKTGGRRGLATMCVGVGQGLALAIER
CHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]