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The map label for this gene is metG [H]

Identifier: 66047113

GI number: 66047113

Start: 4626531

End: 4628579

Strand: Direct

Name: metG [H]

Synonym: Psyr_3886

Alternate gene names: 66047113

Gene position: 4626531-4628579 (Clockwise)

Preceding gene: 66047111

Following gene: 66047114

Centisome position: 75.92

GC content: 60.42

Gene sequence:

>2049_bases
ATGTCCGAGCCACGCAAGATTCTCGTTACCAGCGCCCTGCCCTATGCCAATGGTTCCATTCACCTTGGCCACATGCTCGA
GTACATCCAGACCGACATGTGGGTGCGTTTTCAGAAGCACCGCGGCAATCAATGCATTTATGTCTGCGCGGACGACGCCC
ATGGCTCGGCAATCATGCTGCGCGCCGAAAAGGAAGGCATTACGCCAGAGCAACTGATCGCCAACGTCAAGGCCGAGCAC
AGCGCCGACTTTGCCGACTTCCTGGTGGAATTCGATAATTTCCACTCGACGCACTCGGACGAGAACCGCGAGCTGTCGAG
CATGATCTACAAGCGTCTGCGCGATGCCGGTCATATCGCGACGCGCTCGGTCACCCAGTACTTCGACCCGGAAAAGAAAA
TGTTCCTGGCCGACCGTTTCATCAAGGGCACCTGCCCGAAATGCGCGGCCGAGGACCAGTACGGCGACAACTGTGAAAAG
TGCGGCGCCACCTATGCCCCGACGGACCTCAAAGACCCCAAGTCGGCGATTTCCGGGGCCACGCCGGTGCTCAAGGACTC
GAAGCACTTCTTCTTCGACCTTCCTGCATTCGACGCCATGCTCAAGAGCTGGACACGCAGTGGCACCCTGCAGGACGCCG
TTGCCAACAAGATCGCCGAATGGCTGGACAGCGGCCTGCAGCAGTGGGACATCTCCCGCGATGCGCCCTATTTCGGCTTT
GAAATTCCCGACGAGCCCGGCAAATACTTTTACGTGTGGCTCGATGCGCCGATCGGCTACATGGCCAGCTTCAAGAACCT
GTGCGCGCGTCGTCCGGACCTGGACTTCGATGCTTACTGGGGAAAGGGCGCGACCACCGAGCTCTATCACTTCATCGGCA
AGGACATCGTCAACTTCCATGCGCTGTTCTGGCCAGCCATGCTGGAAGGTGCTGAGTTGCGCACACCGACCGGCATCAAC
GTCCACGGCTACCTGACCGTCAATGGTCAGAAGATGTCGAAGTCGCGTGGCACCTTTATCAAGGCGCGCACCTACCTCGA
CCACCTGCCGCCCGAGTACCTGCGCTATTACTACGCGTCGAAGCTGGGCCGTGGCGTCGACGACCTGGACCTGAACCTCG
AAGACTTCGTGCAAAAGGTCAACTCCGACCTGATCGGCAAGGTCGTCAACATCGCCAGCCGTTGCGCCGGGTTCATTCAC
AAGGGCAACGCCGGTGTGATGGTCGAAGCCAATGCGGCGCCCGAACTGACCGACGCGTTTCTGACGGCTGCACCCAGCAT
CGCCGACGCCTACGAAGCCCGTGACTTCGCCCGCGCCATGCGCGAGACCATGGCCCTGGCGGACCGTGCCAATGCCTATA
TCGCGGAAAAGGCGCCATGGGCGCTGGCCAAACAGGAAGGCAGGCAGGATGAAGTCCAGGCCGTCTGTGCGCTGGGCATC
AACCTGTTCCGCCAACTGGTCATTTTCCTCAAGCCGGTCCTGCCGAATCTGGCCGCCGATGCAGAAAAATTCCTCAACGT
CGAACCACTGACCTGGGAAGACCACAAGACCCTGCTGGCCAATCATCAGCTCAACCCTTTCTCGGCACTGATGACCCGTA
TTGATCCGGTCAAGGTCGAGGCCATGGCCACTGCCTCCAAGGAAGACCTGACCGCTACCGACTCGTCGGCGGACACCGCG
CCTGCCGGTAATGGCGAACTCGCCAAGGACCCGCTGTCGGCGGAAATCGACTTTGACGCCTTTGCCGCCATCGACCTGCG
CGTCGCGCTGATTCTCAAGGCCGAACATGTAGAGGGTGCCGACAAACTGCTGCGCCTGACCCTGGACATCGGCGACGAGC
AGCGCAATGTCTTTTCCGGCATCAAGAGCGCCTACCCGAACCCGTCCGAACTCGAAGGCCGTCTGACCATGATGATCGCC
AACCTCAAGCCGCGAAAAATGCGTTTCGGCATTTCGCAGGGCATGGTGATGGCCGCAGGCCCCGGCGGTGAGGAGATCTA
TCTGCTCAGCCCGGACAGCGGCGCCAGGCCAGGCCAGCGCATCAAGTAA

Upstream 100 bases:

>100_bases
CCCAGCCTTTCAGGAGCATTCCCACAACCGCACGGGTGAAAAAAATCGTTCAGACCTTTATAGTGGCCGGTCTCCGTTTC
ACTTCAAGTAGCCGAGCCCC

Downstream 100 bases:

>100_bases
ACGACGCGAGGCCCGGTATCCTGCCATCAGGCGGATACCGGGCCTTTGCGCATTCGCAAGCTCCCTGATCTGTCCGATAA
TGCTGCTCTCTCGATAGACA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 682; Mature: 681

Protein sequence:

>682_residues
MSEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIMLRAEKEGITPEQLIANVKAEH
SADFADFLVEFDNFHSTHSDENRELSSMIYKRLRDAGHIATRSVTQYFDPEKKMFLADRFIKGTCPKCAAEDQYGDNCEK
CGATYAPTDLKDPKSAISGATPVLKDSKHFFFDLPAFDAMLKSWTRSGTLQDAVANKIAEWLDSGLQQWDISRDAPYFGF
EIPDEPGKYFYVWLDAPIGYMASFKNLCARRPDLDFDAYWGKGATTELYHFIGKDIVNFHALFWPAMLEGAELRTPTGIN
VHGYLTVNGQKMSKSRGTFIKARTYLDHLPPEYLRYYYASKLGRGVDDLDLNLEDFVQKVNSDLIGKVVNIASRCAGFIH
KGNAGVMVEANAAPELTDAFLTAAPSIADAYEARDFARAMRETMALADRANAYIAEKAPWALAKQEGRQDEVQAVCALGI
NLFRQLVIFLKPVLPNLAADAEKFLNVEPLTWEDHKTLLANHQLNPFSALMTRIDPVKVEAMATASKEDLTATDSSADTA
PAGNGELAKDPLSAEIDFDAFAAIDLRVALILKAEHVEGADKLLRLTLDIGDEQRNVFSGIKSAYPNPSELEGRLTMMIA
NLKPRKMRFGISQGMVMAAGPGGEEIYLLSPDSGARPGQRIK

Sequences:

>Translated_682_residues
MSEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIMLRAEKEGITPEQLIANVKAEH
SADFADFLVEFDNFHSTHSDENRELSSMIYKRLRDAGHIATRSVTQYFDPEKKMFLADRFIKGTCPKCAAEDQYGDNCEK
CGATYAPTDLKDPKSAISGATPVLKDSKHFFFDLPAFDAMLKSWTRSGTLQDAVANKIAEWLDSGLQQWDISRDAPYFGF
EIPDEPGKYFYVWLDAPIGYMASFKNLCARRPDLDFDAYWGKGATTELYHFIGKDIVNFHALFWPAMLEGAELRTPTGIN
VHGYLTVNGQKMSKSRGTFIKARTYLDHLPPEYLRYYYASKLGRGVDDLDLNLEDFVQKVNSDLIGKVVNIASRCAGFIH
KGNAGVMVEANAAPELTDAFLTAAPSIADAYEARDFARAMRETMALADRANAYIAEKAPWALAKQEGRQDEVQAVCALGI
NLFRQLVIFLKPVLPNLAADAEKFLNVEPLTWEDHKTLLANHQLNPFSALMTRIDPVKVEAMATASKEDLTATDSSADTA
PAGNGELAKDPLSAEIDFDAFAAIDLRVALILKAEHVEGADKLLRLTLDIGDEQRNVFSGIKSAYPNPSELEGRLTMMIA
NLKPRKMRFGISQGMVMAAGPGGEEIYLLSPDSGARPGQRIK
>Mature_681_residues
SEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIMLRAEKEGITPEQLIANVKAEHS
ADFADFLVEFDNFHSTHSDENRELSSMIYKRLRDAGHIATRSVTQYFDPEKKMFLADRFIKGTCPKCAAEDQYGDNCEKC
GATYAPTDLKDPKSAISGATPVLKDSKHFFFDLPAFDAMLKSWTRSGTLQDAVANKIAEWLDSGLQQWDISRDAPYFGFE
IPDEPGKYFYVWLDAPIGYMASFKNLCARRPDLDFDAYWGKGATTELYHFIGKDIVNFHALFWPAMLEGAELRTPTGINV
HGYLTVNGQKMSKSRGTFIKARTYLDHLPPEYLRYYYASKLGRGVDDLDLNLEDFVQKVNSDLIGKVVNIASRCAGFIHK
GNAGVMVEANAAPELTDAFLTAAPSIADAYEARDFARAMRETMALADRANAYIAEKAPWALAKQEGRQDEVQAVCALGIN
LFRQLVIFLKPVLPNLAADAEKFLNVEPLTWEDHKTLLANHQLNPFSALMTRIDPVKVEAMATASKEDLTATDSSADTAP
AGNGELAKDPLSAEIDFDAFAAIDLRVALILKAEHVEGADKLLRLTLDIGDEQRNVFSGIKSAYPNPSELEGRLTMMIAN
LKPRKMRFGISQGMVMAAGPGGEEIYLLSPDSGARPGQRIK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=561, Percent_Identity=28.698752228164, Blast_Score=241, Evalue=2e-63,
Organism=Homo sapiens, GI24308436, Length=519, Percent_Identity=25.8188824662813, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4507947, Length=157, Percent_Identity=33.1210191082803, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1788432, Length=679, Percent_Identity=63.9175257731959, Blast_Score=935, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=558, Percent_Identity=29.2114695340502, Blast_Score=241, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI212645096, Length=516, Percent_Identity=24.4186046511628, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71992781, Length=343, Percent_Identity=24.4897959183673, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321704, Length=554, Percent_Identity=30.14440433213, Blast_Score=254, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6321610, Length=543, Percent_Identity=22.6519337016575, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24655311, Length=558, Percent_Identity=29.7491039426523, Blast_Score=233, Evalue=4e-61,
Organism=Drosophila melanogaster, GI21358483, Length=511, Percent_Identity=25.6360078277886, Blast_Score=152, Evalue=5e-37,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75688; Mature: 75557

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIML
CCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHEEEHCCCCCEEEEEEECCCCCCEEEE
RAEKEGITPEQLIANVKAEHSADFADFLVEFDNFHSTHSDENRELSSMIYKRLRDAGHIA
EECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHH
TRSVTQYFDPEKKMFLADRFIKGTCPKCAAEDQYGDNCEKCGATYAPTDLKDPKSAISGA
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCC
TPVLKDSKHFFFDLPAFDAMLKSWTRSGTLQDAVANKIAEWLDSGLQQWDISRDAPYFGF
CCCEECCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
EIPDEPGKYFYVWLDAPIGYMASFKNLCARRPDLDFDAYWGKGATTELYHFIGKDIVNFH
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALFWPAMLEGAELRTPTGINVHGYLTVNGQKMSKSRGTFIKARTYLDHLPPEYLRYYYAS
HHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHCCCCCCEEEEHHHHHHCCHHHHHHHHHH
KLGRGVDDLDLNLEDFVQKVNSDLIGKVVNIASRCAGFIHKGNAGVMVEANAAPELTDAF
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCHHHHHH
LTAAPSIADAYEARDFARAMRETMALADRANAYIAEKAPWALAKQEGRQDEVQAVCALGI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCHHHHHHHHHHHH
NLFRQLVIFLKPVLPNLAADAEKFLNVEPLTWEDHKTLLANHQLNPFSALMTRIDPVKVE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCEEEE
AMATASKEDLTATDSSADTAPAGNGELAKDPLSAEIDFDAFAAIDLRVALILKAEHVEGA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEEEEEHHCCCH
DKLLRLTLDIGDEQRNVFSGIKSAYPNPSELEGRLTMMIANLKPRKMRFGISQGMVMAAG
HHEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCEEEEEEECCCCHHHHCCCCCCEEEEEC
PGGEEIYLLSPDSGARPGQRIK
CCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIML
CCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHEEEHCCCCCEEEEEEECCCCCCEEEE
RAEKEGITPEQLIANVKAEHSADFADFLVEFDNFHSTHSDENRELSSMIYKRLRDAGHIA
EECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHH
TRSVTQYFDPEKKMFLADRFIKGTCPKCAAEDQYGDNCEKCGATYAPTDLKDPKSAISGA
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCC
TPVLKDSKHFFFDLPAFDAMLKSWTRSGTLQDAVANKIAEWLDSGLQQWDISRDAPYFGF
CCCEECCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
EIPDEPGKYFYVWLDAPIGYMASFKNLCARRPDLDFDAYWGKGATTELYHFIGKDIVNFH
CCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALFWPAMLEGAELRTPTGINVHGYLTVNGQKMSKSRGTFIKARTYLDHLPPEYLRYYYAS
HHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHCCCCCCEEEEHHHHHHCCHHHHHHHHHH
KLGRGVDDLDLNLEDFVQKVNSDLIGKVVNIASRCAGFIHKGNAGVMVEANAAPELTDAF
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCHHHHHH
LTAAPSIADAYEARDFARAMRETMALADRANAYIAEKAPWALAKQEGRQDEVQAVCALGI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCHHHHHHHHHHHH
NLFRQLVIFLKPVLPNLAADAEKFLNVEPLTWEDHKTLLANHQLNPFSALMTRIDPVKVE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCEEEE
AMATASKEDLTATDSSADTAPAGNGELAKDPLSAEIDFDAFAAIDLRVALILKAEHVEGA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEEEEEHHCCCH
DKLLRLTLDIGDEQRNVFSGIKSAYPNPSELEGRLTMMIANLKPRKMRFGISQGMVMAAG
HHEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCEEEEEEECCCCHHHHCCCCCCEEEEEC
PGGEEIYLLSPDSGARPGQRIK
CCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA