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The map label for this gene is rumA [H]

Identifier: 66046925

GI number: 66046925

Start: 4383042

End: 4384397

Strand: Reverse

Name: rumA [H]

Synonym: Psyr_3696

Alternate gene names: 66046925

Gene position: 4384397-4383042 (Counterclockwise)

Preceding gene: 66046926

Following gene: 66046924

Centisome position: 71.95

GC content: 61.58

Gene sequence:

>1356_bases
ATGGCCAAGCATGAAAGAGGTTTGCGCTTTCAGCCTGCGGGCGGCGTTAAAACCGTACAGATTCCTGCTGGCAAAAAGCA
GCGGCTGACCATTGAGCGGCTGTCCGACGACGGTCGCGGTATCGCCTTTCTCGAGGGTAAAACCTGGTTTGTCGCCGGTA
GTCTGGCCGGCGAAGAGGTCGAGGCGCGCGTGCTCAATGCGCGCGGCAAGGTCGTCGAGGCGCGCACCGAGCGGGTGTTC
ACTGCCAGCGCCATGCGTCGCGCTCCGGCCTGTGAGTATTTCGGTCGCTGCGGCGGCTGCAGTGTTCAGCATGTGCCCCA
TGAGGAACAGCTTGCCCTGAAACAGCGCATGCTCGCCGAGCAGTTGCTGCGCGTGGCGAACGTGGTGCCGGATGAATGGG
CGGCACCTTTGAGTGGTGCCGAGCTGGCCTATCGCCGACGCGCGCGGGTCGCGGTTCGCTGGGATGCCAAGGCCAAAAGG
CTGGATGTGGGGTTCCGCGCAGCCGCCAGCCAGGACATCGTCAGCATCGAACACTGTCCAGTGCTGGTACAAGCCTTGCA
ACCGATCATGAATGAGTTGCCTGGCATGCTGAAAAGTTTCAGCAAGCCGCAGGTGCTGGGTCACGTCGAGCTGTTCAGTG
GGGTGGCGACTGCCGTGTTGTTGCGCCACACCGCGTCGCTTGTCGAGGCCGACCTGGCGCTGTTGCAGGCGTTTTGCTCC
AGGCATGGCGCGCAGCTGTGGCTGCACGGCGAAGGCGAGCCGCAACCGGCCAGCCCCGGCGATACGCTGGGCTACCGGCT
GGAGCCGTGGAACCTGCAACTGGCCTGGCGACCGGGAGATTTCATTCAGGTCAACGCGTCGGTCAATACCGCGATGATCG
AGCAGGCCCTGCAATGGCTGGCGCCTGCAAAAGATGAGCGTGTCATGGATCTGTTCTGTGGTCTGGGCAATTTCGCCTTG
CCACTGGCGGGGCTGGCCAGGGACGTGGTGGCGGTCGAAGGCGTTGCGACCATGGTCGAGCGTGCAGCCGTCAACGCAAT
GAGTAATGATCTGCACAATGTGCGGTTTTTTCAGGCGGATCTTTCCCAGCCGCTGACACAGGCCGACTGGGCGGCAGAGG
GGTTTTCTGCGGTACTCTTGGACCCACCGCGTGACGGAGCCTTTGAAGTGGTCCGCCAGATCAGAAAGACGGGGGCCCGG
CGACTGCTTTATGTTTCATGCAACCCGGCAACTTTGGCTCGTGACGCGGTCGAACTGATCCGTCAGGGCTACCGATTAAA
ACGTGCCGGAATCCTCGATATGTTTCCTCAGACGGCGCATGTCGAGGCGATGGCTTTATTTGAAATGAGTAAGTGA

Upstream 100 bases:

>100_bases
TGCGTATTTCCCGGGAAGTCGAAAACGCCGTGATTGTGGCGATCATCTGTGACCGAGGCGACCGTTACCTGTCGACCGGC
GTCTATGATTCGCCCAACTG

Downstream 100 bases:

>100_bases
GCGGCGTTCACTGGAACGAGTTCGTTGCTGACAAGGAATCTCATTTAATCCGACTGGCCCGCGAATGCCGGTCCTTGCTT
GCCCGGCAAGTGGCATTCGC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MAKHERGLRFQPAGGVKTVQIPAGKKQRLTIERLSDDGRGIAFLEGKTWFVAGSLAGEEVEARVLNARGKVVEARTERVF
TASAMRRAPACEYFGRCGGCSVQHVPHEEQLALKQRMLAEQLLRVANVVPDEWAAPLSGAELAYRRRARVAVRWDAKAKR
LDVGFRAAASQDIVSIEHCPVLVQALQPIMNELPGMLKSFSKPQVLGHVELFSGVATAVLLRHTASLVEADLALLQAFCS
RHGAQLWLHGEGEPQPASPGDTLGYRLEPWNLQLAWRPGDFIQVNASVNTAMIEQALQWLAPAKDERVMDLFCGLGNFAL
PLAGLARDVVAVEGVATMVERAAVNAMSNDLHNVRFFQADLSQPLTQADWAAEGFSAVLLDPPRDGAFEVVRQIRKTGAR
RLLYVSCNPATLARDAVELIRQGYRLKRAGILDMFPQTAHVEAMALFEMSK

Sequences:

>Translated_451_residues
MAKHERGLRFQPAGGVKTVQIPAGKKQRLTIERLSDDGRGIAFLEGKTWFVAGSLAGEEVEARVLNARGKVVEARTERVF
TASAMRRAPACEYFGRCGGCSVQHVPHEEQLALKQRMLAEQLLRVANVVPDEWAAPLSGAELAYRRRARVAVRWDAKAKR
LDVGFRAAASQDIVSIEHCPVLVQALQPIMNELPGMLKSFSKPQVLGHVELFSGVATAVLLRHTASLVEADLALLQAFCS
RHGAQLWLHGEGEPQPASPGDTLGYRLEPWNLQLAWRPGDFIQVNASVNTAMIEQALQWLAPAKDERVMDLFCGLGNFAL
PLAGLARDVVAVEGVATMVERAAVNAMSNDLHNVRFFQADLSQPLTQADWAAEGFSAVLLDPPRDGAFEVVRQIRKTGAR
RLLYVSCNPATLARDAVELIRQGYRLKRAGILDMFPQTAHVEAMALFEMSK
>Mature_450_residues
AKHERGLRFQPAGGVKTVQIPAGKKQRLTIERLSDDGRGIAFLEGKTWFVAGSLAGEEVEARVLNARGKVVEARTERVFT
ASAMRRAPACEYFGRCGGCSVQHVPHEEQLALKQRMLAEQLLRVANVVPDEWAAPLSGAELAYRRRARVAVRWDAKAKRL
DVGFRAAASQDIVSIEHCPVLVQALQPIMNELPGMLKSFSKPQVLGHVELFSGVATAVLLRHTASLVEADLALLQAFCSR
HGAQLWLHGEGEPQPASPGDTLGYRLEPWNLQLAWRPGDFIQVNASVNTAMIEQALQWLAPAKDERVMDLFCGLGNFALP
LAGLARDVVAVEGVATMVERAAVNAMSNDLHNVRFFQADLSQPLTQADWAAEGFSAVLLDPPRDGAFEVVRQIRKTGARR
LLYVSCNPATLARDAVELIRQGYRLKRAGILDMFPQTAHVEAMALFEMSK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=191, Percent_Identity=36.1256544502618, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI51173878, Length=191, Percent_Identity=36.1256544502618, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1789148, Length=428, Percent_Identity=40.1869158878505, Blast_Score=308, Evalue=5e-85,
Organism=Escherichia coli, GI1787083, Length=383, Percent_Identity=27.9373368146214, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790403, Length=373, Percent_Identity=24.9329758713137, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24666579, Length=195, Percent_Identity=31.7948717948718, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 49509; Mature: 49377

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHERGLRFQPAGGVKTVQIPAGKKQRLTIERLSDDGRGIAFLEGKTWFVAGSLAGEEV
CCCCCCCCEECCCCCEEEEEECCCCCCEEEHEECCCCCCEEEEEECCEEEEEECCCCCHH
EARVLNARGKVVEARTERVFTASAMRRAPACEYFGRCGGCSVQHVPHEEQLALKQRMLAE
HHHHHHCCCCEEEHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
QLLRVANVVPDEWAAPLSGAELAYRRRARVAVRWDAKAKRLDVGFRAAASQDIVSIEHCP
HHHHHHHCCCCHHCCCCCCHHHHHHHHCCEEEEECCCCHHCCCCHHHCCCCCCEEHHHHH
VLVQALQPIMNELPGMLKSFSKPQVLGHVELFSGVATAVLLRHTASLVEADLALLQAFCS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHGAQLWLHGEGEPQPASPGDTLGYRLEPWNLQLAWRPGDFIQVNASVNTAMIEQALQWL
CCCCEEEEECCCCCCCCCCCCCCCEEECCCEEEEEECCCCEEEEECCCCHHHHHHHHHHH
APAKDERVMDLFCGLGNFALPLAGLARDVVAVEGVATMVERAAVNAMSNDLHNVRFFQAD
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHH
LSQPLTQADWAAEGFSAVLLDPPRDGAFEVVRQIRKTGARRLLYVSCNPATLARDAVELI
HCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
RQGYRLKRAGILDMFPQTAHVEAMALFEMSK
HCCCHHHHCCHHHHCCCCHHHHHHHHEECCC
>Mature Secondary Structure 
AKHERGLRFQPAGGVKTVQIPAGKKQRLTIERLSDDGRGIAFLEGKTWFVAGSLAGEEV
CCCCCCCEECCCCCEEEEEECCCCCCEEEHEECCCCCCEEEEEECCEEEEEECCCCCHH
EARVLNARGKVVEARTERVFTASAMRRAPACEYFGRCGGCSVQHVPHEEQLALKQRMLAE
HHHHHHCCCCEEEHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
QLLRVANVVPDEWAAPLSGAELAYRRRARVAVRWDAKAKRLDVGFRAAASQDIVSIEHCP
HHHHHHHCCCCHHCCCCCCHHHHHHHHCCEEEEECCCCHHCCCCHHHCCCCCCEEHHHHH
VLVQALQPIMNELPGMLKSFSKPQVLGHVELFSGVATAVLLRHTASLVEADLALLQAFCS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHGAQLWLHGEGEPQPASPGDTLGYRLEPWNLQLAWRPGDFIQVNASVNTAMIEQALQWL
CCCCEEEEECCCCCCCCCCCCCCCEEECCCEEEEEECCCCEEEEECCCCHHHHHHHHHHH
APAKDERVMDLFCGLGNFALPLAGLARDVVAVEGVATMVERAAVNAMSNDLHNVRFFQAD
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHH
LSQPLTQADWAAEGFSAVLLDPPRDGAFEVVRQIRKTGARRLLYVSCNPATLARDAVELI
HCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
RQGYRLKRAGILDMFPQTAHVEAMALFEMSK
HCCCHHHHCCHHHHCCCCHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA