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The map label for this gene is purB [H]

Identifier: 66046421

GI number: 66046421

Start: 3821398

End: 3822816

Strand: Direct

Name: purB [H]

Synonym: Psyr_3192

Alternate gene names: 66046421

Gene position: 3821398-3822816 (Clockwise)

Preceding gene: 66046420

Following gene: 66046422

Centisome position: 62.71

GC content: 60.04

Gene sequence:

>1419_bases
ATGTATGATATGCGCCCTTTTCGTCGCCCGACTGTCCGAGAGCATCCCATGCAGCTTTCTTCGCTCACTGCGGTTTCCCC
TGTTGATGGCCGCTACGCCGGCAAAACCCAGGCCTTGCGCCCTATTTTCAGCGAATACGGCCTGATCCGTTTCCGCGTGA
TGGTTGAAGTTCGCTGGCTCCAGCGTCTTGCGGCTCACCCGCAGATCACCGAAGTCCCGGCCTTCTCCGCGCAAGCCAAC
GCCATCCTGGATGCCCTGGCTGAAGACTTCTCCGTGGAGCATGCCGAGCGCGTCAAGGAAATCGAGCGCACCACCAATCA
TGACGTGAAGGCTGTGGAGTATCTGCTCAAGGAGCAGGCTGCCAAATTGCCAGAGCTGGAAAAGGTCAGTGAGTTCATCC
ACTTCGCCTGCACCAGCGAAGACATCAACAACCTGTCTCACGCATTGATGCTGCGCGAGGGCCGCGACACTGTCGTGCTG
CCGCTGATGCGCCAGATCGCCGACGCCATCCGCGAGCTGGCGATTCGCTTCGCAGACGTGCCGATGCTGTCCCGCACCCA
CGGCCAGCCCGCCTCCCCGACCACACTGGGCAAGGAACTGGCCAACGTCGTCTACCGCCTCGAGCGCCAGATCAGCCAGA
TCGCGGCGGTCCCGTTGCTCGGCAAGATCAACGGCGCAGTCGGCAACTACAATGCCCACCTCTCTGCCTACCCAGACATC
GACTGGGAAGCCAACGCGCGCGCCTTCATCGAAGACGAACTGGGCCTGGGCTTCAACCCCTACACCACGCAGATCGAACC
GCACGACTACATTGCCGAGCTGTTCGATGCCATTGCGCGCTTCAACACCATCCTGATCGACTTCGACCGTGACATCTGGG
GCTACATCTCGCTGGGCTATTTCAAACAGCGCACCATCGCTGGCGAAATCGGCTCCTCGACCATGCCGCACAAGGTCAAC
CCGATCGACTTCGAAAACTCCGAAGGCAACCTGGGCATCGCCAACGCACTGTTCCAGCATCTGGCGAGCAAGCTGCCTGT
CTCTCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTCGGCTTTGCCCACAGCGTCATTGCCT
ACGAAGCCAGCCTCAAGGGCATCAGCAAGCTGGAACTGAACGAACAGCGCATTGCCGCCGATCTGGACGCATGCTGGGAA
GTTCTGGCAGAGCCGATTCAGACTGTCATGCGTCGGTACAACATCGAAAACCCGTACGAAAAACTGAAAGAACTGACCCG
CGGCAAGGGCATCGGTCCTGAAGCGTTGCAGACTTTCATCGACGGTCTGGACATGCCCTCCGAAGCCAAGGCCGAGCTCA
AGAAGCTCACGCCAGCCAATTACATCGGTAACGCAGCCGCTCAGGCCAAGCGCATCTGA

Upstream 100 bases:

>100_bases
CATTGCTGCACGGCTGACCCGCCGCGCGCCAATGACCTTCACCACCCCAGGCACGACGCTGTCTACGCTGGTCAGCGGGC
TCCGGTCCGACTGACTTTTC

Downstream 100 bases:

>100_bases
CACGCAAAAAAATTGCACACGCCCGGCTGTGCCGGGCGTTTTCATTTCAAGGGCTTACACATGAATCCTGATATTCCTCT
ACAACTTCTGGGCGGCATCA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MYDMRPFRRPTVREHPMQLSSLTAVSPVDGRYAGKTQALRPIFSEYGLIRFRVMVEVRWLQRLAAHPQITEVPAFSAQAN
AILDALAEDFSVEHAERVKEIERTTNHDVKAVEYLLKEQAAKLPELEKVSEFIHFACTSEDINNLSHALMLREGRDTVVL
PLMRQIADAIRELAIRFADVPMLSRTHGQPASPTTLGKELANVVYRLERQISQIAAVPLLGKINGAVGNYNAHLSAYPDI
DWEANARAFIEDELGLGFNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDIWGYISLGYFKQRTIAGEIGSSTMPHKVN
PIDFENSEGNLGIANALFQHLASKLPVSRWQRDLTDSTVLRNLGVGFAHSVIAYEASLKGISKLELNEQRIAADLDACWE
VLAEPIQTVMRRYNIENPYEKLKELTRGKGIGPEALQTFIDGLDMPSEAKAELKKLTPANYIGNAAAQAKRI

Sequences:

>Translated_472_residues
MYDMRPFRRPTVREHPMQLSSLTAVSPVDGRYAGKTQALRPIFSEYGLIRFRVMVEVRWLQRLAAHPQITEVPAFSAQAN
AILDALAEDFSVEHAERVKEIERTTNHDVKAVEYLLKEQAAKLPELEKVSEFIHFACTSEDINNLSHALMLREGRDTVVL
PLMRQIADAIRELAIRFADVPMLSRTHGQPASPTTLGKELANVVYRLERQISQIAAVPLLGKINGAVGNYNAHLSAYPDI
DWEANARAFIEDELGLGFNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDIWGYISLGYFKQRTIAGEIGSSTMPHKVN
PIDFENSEGNLGIANALFQHLASKLPVSRWQRDLTDSTVLRNLGVGFAHSVIAYEASLKGISKLELNEQRIAADLDACWE
VLAEPIQTVMRRYNIENPYEKLKELTRGKGIGPEALQTFIDGLDMPSEAKAELKKLTPANYIGNAAAQAKRI
>Mature_472_residues
MYDMRPFRRPTVREHPMQLSSLTAVSPVDGRYAGKTQALRPIFSEYGLIRFRVMVEVRWLQRLAAHPQITEVPAFSAQAN
AILDALAEDFSVEHAERVKEIERTTNHDVKAVEYLLKEQAAKLPELEKVSEFIHFACTSEDINNLSHALMLREGRDTVVL
PLMRQIADAIRELAIRFADVPMLSRTHGQPASPTTLGKELANVVYRLERQISQIAAVPLLGKINGAVGNYNAHLSAYPDI
DWEANARAFIEDELGLGFNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDIWGYISLGYFKQRTIAGEIGSSTMPHKVN
PIDFENSEGNLGIANALFQHLASKLPVSRWQRDLTDSTVLRNLGVGFAHSVIAYEASLKGISKLELNEQRIAADLDACWE
VLAEPIQTVMRRYNIENPYEKLKELTRGKGIGPEALQTFIDGLDMPSEAKAELKKLTPANYIGNAAAQAKRI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=449, Percent_Identity=71.0467706013363, Blast_Score=686, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 52951; Mature: 52951

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDMRPFRRPTVREHPMQLSSLTAVSPVDGRYAGKTQALRPIFSEYGLIRFRVMVEVRWL
CCCCCCCCCCCCHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
QRLAAHPQITEVPAFSAQANAILDALAEDFSVEHAERVKEIERTTNHDVKAVEYLLKEQA
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AKLPELEKVSEFIHFACTSEDINNLSHALMLREGRDTVVLPLMRQIADAIRELAIRFADV
HCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PMLSRTHGQPASPTTLGKELANVVYRLERQISQIAAVPLLGKINGAVGNYNAHLSAYPDI
CHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
DWEANARAFIEDELGLGFNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDIWGYISLGY
CCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHH
FKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGIANALFQHLASKLPVSRWQRDLTDSTVL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RNLGVGFAHSVIAYEASLKGISKLELNEQRIAADLDACWEVLAEPIQTVMRRYNIENPYE
HHHCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KLKELTRGKGIGPEALQTFIDGLDMPSEAKAELKKLTPANYIGNAAAQAKRI
HHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MYDMRPFRRPTVREHPMQLSSLTAVSPVDGRYAGKTQALRPIFSEYGLIRFRVMVEVRWL
CCCCCCCCCCCCHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
QRLAAHPQITEVPAFSAQANAILDALAEDFSVEHAERVKEIERTTNHDVKAVEYLLKEQA
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AKLPELEKVSEFIHFACTSEDINNLSHALMLREGRDTVVLPLMRQIADAIRELAIRFADV
HCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PMLSRTHGQPASPTTLGKELANVVYRLERQISQIAAVPLLGKINGAVGNYNAHLSAYPDI
CHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
DWEANARAFIEDELGLGFNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDIWGYISLGY
CCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHH
FKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGIANALFQHLASKLPVSRWQRDLTDSTVL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RNLGVGFAHSVIAYEASLKGISKLELNEQRIAADLDACWEVLAEPIQTVMRRYNIENPYE
HHHCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KLKELTRGKGIGPEALQTFIDGLDMPSEAKAELKKLTPANYIGNAAAQAKRI
HHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]