BacMap

Definition	Pseudomonas syringae pv. syringae B728a, complete genome.
Accession	NC_007005
Length	6,093,698

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The map label for this gene is fadB1 [H]

Identifier: 66046261

GI number: 66046261

Start: 3628614

End: 3629387

Strand: Reverse

Name: fadB1 [H]

Synonym: Psyr_3030

Alternate gene names: 66046261

Gene position: 3629387-3628614 (Counterclockwise)

Preceding gene: 66046262

Following gene: 66046259

Centisome position: 59.56

GC content: 61.11

Gene sequence:

>774_bases
ATGCTTTACGAGACTATTCTCATTGAGATTATAGGTAGTGTGGGCCTGATCAGGCTTAACCGGCCTAACGCGTTGAACGC
CTTGAATGCGCGTCTGATCAACGAATTGAACCATGCGCTCGATCAGTTCGAGGCGGACAGTGCGATCGGCTGCATCGTGC
TGACCGGCTCGGAAAAAGCCTTCGCCGCCGGTGCGGACATCAAGGAAATGGTCGACCTGTCCTACCCGCAGATCTACCTC
GATGACCTGTTTCGTGACAGCGACCGGGTTGCGGCCCGACGCAAACCCCTGGTGGCTGCGGTGGCCGGTTTTGCCTTGGG
TGGCGGCTGTGAACTGGCATTGATGTGCGATTTCATTCTGGCTGCCGAGAACGCCAGGTTTGGACAGCCCGAGATCAATC
TCGGCGTGTTGCCGGGGATGGGCGGAACGCAGCGTCTGACCCGCGCGGTGGGCAAGGCCAAGGCCATGGAGATGTGCCTG
ACCGGCCGGTTGATCGATGCTCACGAGGCCGAGCGCGCCGGTCTGGTGGCCCGCGTGCTGCCGCTCGATCAGTTGCTGCC
GGAAGCGCTTGCAGTGGCAGCGGTCATCGCCAGTAAATCCCTGCCAGTAGCGATGATGGTCAAAGAGAGCGTCAACCGCG
CGTTCGAGGTCAGCCTGGCCGAAGGCATCCGCTTCGAGCGCCGGGTTTTTCACGCCGCCTTCGCCAGCCAGGATCAGAAG
GAGGGCATGCGCGCCTTCATCGACAAGCGCCAGCCCGATTTCAAGGACTGCTGA

Upstream 100 bases:

>100_bases
TTTGAATTAGCCTTATTAATCAGTAGCTTGATCTAATAATTGAATGTTGAATCTATAAAAATCTGCTGATTGCATAATTA
AATAAAAAGGATATTTATCA

Downstream 100 bases:

>100_bases
TCTGCATGGGCAACGATCAGAATGCGTTGCGCGTCGACCCGCAACTCGGCGGTCCGCCCGCGCACTGTCAATCCGTCATG
CGCGTTGATGTGCAGGCGCT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLYETILIEIIGSVGLIRLNRPNALNALNARLINELNHALDQFEADSAIGCIVLTGSEKAFAAGADIKEMVDLSYPQIYL
DDLFRDSDRVAARRKPLVAAVAGFALGGGCELALMCDFILAAENARFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCL
TGRLIDAHEAERAGLVARVLPLDQLLPEALAVAAVIASKSLPVAMMVKESVNRAFEVSLAEGIRFERRVFHAAFASQDQK
EGMRAFIDKRQPDFKDC

Sequences:

>Translated_257_residues
MLYETILIEIIGSVGLIRLNRPNALNALNARLINELNHALDQFEADSAIGCIVLTGSEKAFAAGADIKEMVDLSYPQIYL
DDLFRDSDRVAARRKPLVAAVAGFALGGGCELALMCDFILAAENARFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCL
TGRLIDAHEAERAGLVARVLPLDQLLPEALAVAAVIASKSLPVAMMVKESVNRAFEVSLAEGIRFERRVFHAAFASQDQK
EGMRAFIDKRQPDFKDC
>Mature_257_residues
MLYETILIEIIGSVGLIRLNRPNALNALNARLINELNHALDQFEADSAIGCIVLTGSEKAFAAGADIKEMVDLSYPQIYL
DDLFRDSDRVAARRKPLVAAVAGFALGGGCELALMCDFILAAENARFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCL
TGRLIDAHEAERAGLVARVLPLDQLLPEALAVAAVIASKSLPVAMMVKESVNRAFEVSLAEGIRFERRVFHAAFASQDQK
EGMRAFIDKRQPDFKDC

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=55.6420233463035, Blast_Score=286, Evalue=1e-77,
Organism=Homo sapiens, GI4502327, Length=244, Percent_Identity=36.0655737704918, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI68989263, Length=189, Percent_Identity=37.037037037037, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=37.3626373626374, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI31542718, Length=242, Percent_Identity=28.099173553719, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=28.4, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI150378541, Length=243, Percent_Identity=31.6872427983539, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI37594471, Length=188, Percent_Identity=31.3829787234043, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI37594469, Length=188, Percent_Identity=31.3829787234043, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=27.0742358078603, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=27.0742358078603, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI45643119, Length=244, Percent_Identity=27.4590163934426, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=244, Percent_Identity=27.4590163934426, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757968, Length=249, Percent_Identity=25.3012048192771, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI49355787, Length=249, Percent_Identity=25.3012048192771, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260274832, Length=244, Percent_Identity=27.4590163934426, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI25453481, Length=249, Percent_Identity=24.8995983935743, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI51538573, Length=249, Percent_Identity=24.8995983935743, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI62530384, Length=210, Percent_Identity=29.0476190476191, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136756, Length=240, Percent_Identity=25.8333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=240, Percent_Identity=25.8333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=166, Percent_Identity=30.1204819277108, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI4757966, Length=175, Percent_Identity=28, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI51538581, Length=175, Percent_Identity=28, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI157694520, Length=191, Percent_Identity=28.2722513089005, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI261878539, Length=97, Percent_Identity=40.2061855670103, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=243, Percent_Identity=46.9135802469136, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=34.8659003831418, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082183, Length=261, Percent_Identity=32.183908045977, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=33.8709677419355, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=33.206106870229, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1790281, Length=200, Percent_Identity=33, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=52.34375, Blast_Score=280, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=51.2396694214876, Blast_Score=252, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17558304, Length=190, Percent_Identity=34.7368421052632, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=243, Percent_Identity=32.0987654320988, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=29.5964125560538, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=198, Percent_Identity=36.3636363636364, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=208, Percent_Identity=35.0961538461538, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=198, Percent_Identity=36.3636363636364, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17540306, Length=238, Percent_Identity=31.5126050420168, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=261, Percent_Identity=28.735632183908, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=191, Percent_Identity=31.413612565445, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=191, Percent_Identity=31.413612565445, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=238, Percent_Identity=29.4117647058824, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=227, Percent_Identity=25.9911894273128, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=207, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=26.9430051813472, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20129971, Length=257, Percent_Identity=56.420233463035, Blast_Score=297, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24653477, Length=257, Percent_Identity=56.420233463035, Blast_Score=297, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24653139, Length=249, Percent_Identity=32.9317269076305, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24650670, Length=256, Percent_Identity=34.765625, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=28.515625, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19920382, Length=224, Percent_Identity=34.375, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21357171, Length=264, Percent_Identity=31.4393939393939, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19921000, Length=182, Percent_Identity=34.6153846153846, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24583077, Length=182, Percent_Identity=34.6153846153846, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583079, Length=182, Percent_Identity=34.6153846153846, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571730, Length=227, Percent_Identity=27.7533039647577, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571729, Length=227, Percent_Identity=27.7533039647577, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654903, Length=191, Percent_Identity=25.6544502617801, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583165, Length=165, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27844; Mature: 27844

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYETILIEIIGSVGLIRLNRPNALNALNARLINELNHALDQFEADSAIGCIVLTGSEKA
CHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
FAAGADIKEMVDLSYPQIYLDDLFRDSDRVAARRKPLVAAVAGFALGGGCELALMCDFIL
EECCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHH
AAENARFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCLTGRLIDAHEAERAGLVARVL
HHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHH
PLDQLLPEALAVAAVIASKSLPVAMMVKESVNRAFEVSLAEGIRFERRVFHAAFASQDQK
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEHHHHHCCHHHHHHHHHHHHCCHHH
EGMRAFIDKRQPDFKDC
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLYETILIEIIGSVGLIRLNRPNALNALNARLINELNHALDQFEADSAIGCIVLTGSEKA
CHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
FAAGADIKEMVDLSYPQIYLDDLFRDSDRVAARRKPLVAAVAGFALGGGCELALMCDFIL
EECCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHH
AAENARFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCLTGRLIDAHEAERAGLVARVL
HHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHH
PLDQLLPEALAVAAVIASKSLPVAMMVKESVNRAFEVSLAEGIRFERRVFHAAFASQDQK
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEHHHHHCCHHHHHHHHHHHHCCHHH
EGMRAFIDKRQPDFKDC
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]