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The map label for this gene is thlA [H]

Identifier: 66046262

GI number: 66046262

Start: 3629478

End: 3630686

Strand: Reverse

Name: thlA [H]

Synonym: Psyr_3031

Alternate gene names: 66046262

Gene position: 3630686-3629478 (Counterclockwise)

Preceding gene: 66046267

Following gene: 66046261

Centisome position: 59.58

GC content: 63.19

Gene sequence:

>1209_bases
ATGTCCATCGAAAGGACACCCCCTCATCAAGACCCCGTGGTCATTGTCAGTGCCGTGCGCACGCCCATGGGTGGTTTTCA
GGGCGATCTGCAGAGCCTCAGTGCCACCGCGCTGGGATCGATCGCCATCCGTGCCGCTGTCGAGCGGGCAGGCATCGAGC
CTGCAGACGTGGAGCATGTGCTGTTTGGTTGTGTGCTGCCGGCAGGTCTGGGCCAGGCACCCGCTCGGCAGGCGGCGCTG
GGTGCCGGGCTCAGCCATGCGACGCTGTGCAGCACGGTCAACAAGATGTGCGGTTCCGGCATGCAGAGCGCAATCATGGC
CCATGACCTGTTGCTGGCAGACAGTACTGCGGTGGCGGTGGCTGGCGGCATGGAGAGCATGTCCAATGCGCCTTACCTGC
TGGACCGCGCGCGCAGCGGTTATCGCATGGGGCACGGCAAGGTGCTCGATCACATGTTTCTCGACGGCCTCGAAGATGCC
TATGAGCCAGGACGGCTGATGGGCACCTTCGCCGAGGACTGCGCCGGGCTCAACGGTTTCACTCGCGAGGCGCAGGATGC
GTTTGCCCTGGCCTCACTGGCTCGCGCGCAAAAGGCCATTGCCGAAGGCCATTTCGACGCCGAGATCGTGCCGGTGCAAG
TCACCGTGGGCAAGGAGTCGAGGCAAATCACCCACGATGAGCAGCCACCCAAGGCACGTCCCGACAAGATACCCACGCTC
AAACCCGCTTTTCGCGAGGGCGGCACGGTCACCGCGGCCAACTCCAGTTCTATCTCCGACGGCGCAGCAGCGCTGCTGTT
GATGCGTCAGTCCGAGGCTCAAAGGCGTGGCCTGCGGCCTCTGGCGGTGTTTCATGCGCATGCGGCATTTGCCGACGAGC
CGGGCCTGTTTCCAACAGCGCCGATAGGCGCCATTCAGCGCCTTTTGGGCAAGACAGGCTGGAGCCTGGATGATGTGGAT
CTGTTCGAGATCAACGAAGCCTTTGCGGTGGTGCCACTGGTGGCAATGCGCGAGCTGGGCATCCCGCACGATAAAGTCAA
TGTCAATGGAGGTGCCTGCGCGCTTGGTCATCCGATCGGCGCCTCGGGTGCGCGGATCATCGTGACGCTGCTGGCGGCCT
TGCGGCATCGACAACTCAAGCGTGGCCTGGCGGCGATCTGCATCGGCGGCGGTGAAGCTACTGCGATAGCTATCGAGTTT
TTGAATTAG

Upstream 100 bases:

>100_bases
AATCTGCCTGCCTCAACCATTGCGTCTGCAAGGCAGCAGGTTGATGATTCCCCCGTACCTTTAGAAAACCGTACCGATAA
AAACAAGACTGGAGATAGCC

Downstream 100 bases:

>100_bases
CCTTATTAATCAGTAGCTTGATCTAATAATTGAATGTTGAATCTATAAAAATCTGCTGATTGCATAATTAAATAAAAAGG
ATATTTATCAATGCTTTACG

Product: thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSIERTPPHQDPVVIVSAVRTPMGGFQGDLQSLSATALGSIAIRAAVERAGIEPADVEHVLFGCVLPAGLGQAPARQAAL
GAGLSHATLCSTVNKMCGSGMQSAIMAHDLLLADSTAVAVAGGMESMSNAPYLLDRARSGYRMGHGKVLDHMFLDGLEDA
YEPGRLMGTFAEDCAGLNGFTREAQDAFALASLARAQKAIAEGHFDAEIVPVQVTVGKESRQITHDEQPPKARPDKIPTL
KPAFREGGTVTAANSSSISDGAAALLLMRQSEAQRRGLRPLAVFHAHAAFADEPGLFPTAPIGAIQRLLGKTGWSLDDVD
LFEINEAFAVVPLVAMRELGIPHDKVNVNGGACALGHPIGASGARIIVTLLAALRHRQLKRGLAAICIGGGEATAIAIEF
LN

Sequences:

>Translated_402_residues
MSIERTPPHQDPVVIVSAVRTPMGGFQGDLQSLSATALGSIAIRAAVERAGIEPADVEHVLFGCVLPAGLGQAPARQAAL
GAGLSHATLCSTVNKMCGSGMQSAIMAHDLLLADSTAVAVAGGMESMSNAPYLLDRARSGYRMGHGKVLDHMFLDGLEDA
YEPGRLMGTFAEDCAGLNGFTREAQDAFALASLARAQKAIAEGHFDAEIVPVQVTVGKESRQITHDEQPPKARPDKIPTL
KPAFREGGTVTAANSSSISDGAAALLLMRQSEAQRRGLRPLAVFHAHAAFADEPGLFPTAPIGAIQRLLGKTGWSLDDVD
LFEINEAFAVVPLVAMRELGIPHDKVNVNGGACALGHPIGASGARIIVTLLAALRHRQLKRGLAAICIGGGEATAIAIEF
LN
>Mature_401_residues
SIERTPPHQDPVVIVSAVRTPMGGFQGDLQSLSATALGSIAIRAAVERAGIEPADVEHVLFGCVLPAGLGQAPARQAALG
AGLSHATLCSTVNKMCGSGMQSAIMAHDLLLADSTAVAVAGGMESMSNAPYLLDRARSGYRMGHGKVLDHMFLDGLEDAY
EPGRLMGTFAEDCAGLNGFTREAQDAFALASLARAQKAIAEGHFDAEIVPVQVTVGKESRQITHDEQPPKARPDKIPTLK
PAFREGGTVTAANSSSISDGAAALLLMRQSEAQRRGLRPLAVFHAHAAFADEPGLFPTAPIGAIQRLLGKTGWSLDDVDL
FEINEAFAVVPLVAMRELGIPHDKVNVNGGACALGHPIGASGARIIVTLLAALRHRQLKRGLAAICIGGGEATAIAIEFL
N

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=404, Percent_Identity=46.7821782178218, Blast_Score=321, Evalue=6e-88,
Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=45.1530612244898, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=35.4755784061697, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=36.8686868686869, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI4504327, Length=426, Percent_Identity=31.2206572769953, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=28.7179487179487, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1788554, Length=391, Percent_Identity=50.8951406649616, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI87082165, Length=388, Percent_Identity=42.7835051546392, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI1787663, Length=395, Percent_Identity=36.9620253164557, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=37.3762376237624, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1788683, Length=403, Percent_Identity=30.272952853598, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25147385, Length=387, Percent_Identity=40.8268733850129, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17535921, Length=391, Percent_Identity=42.7109974424552, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI133906874, Length=388, Percent_Identity=38.9175257731959, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17535917, Length=391, Percent_Identity=34.0153452685422, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=30.166270783848, Blast_Score=158, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=393, Percent_Identity=46.3104325699746, Blast_Score=320, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6322031, Length=400, Percent_Identity=33.25, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=44.5012787723785, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24640423, Length=391, Percent_Identity=45.5242966751918, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI17648125, Length=391, Percent_Identity=35.5498721227622, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI17137578, Length=422, Percent_Identity=29.1469194312796, Blast_Score=148, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41933; Mature: 41801

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIERTPPHQDPVVIVSAVRTPMGGFQGDLQSLSATALGSIAIRAAVERAGIEPADVEHV
CCCCCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LFGCVLPAGLGQAPARQAALGAGLSHATLCSTVNKMCGSGMQSAIMAHDLLLADSTAVAV
HHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEE
AGGMESMSNAPYLLDRARSGYRMGHGKVLDHMFLDGLEDAYEPGRLMGTFAEDCAGLNGF
ECCHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
TREAQDAFALASLARAQKAIAEGHFDAEIVPVQVTVGKESRQITHDEQPPKARPDKIPTL
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
KPAFREGGTVTAANSSSISDGAAALLLMRQSEAQRRGLRPLAVFHAHAAFADEPGLFPTA
CHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC
PIGAIQRLLGKTGWSLDDVDLFEINEAFAVVPLVAMRELGIPHDKVNVNGGACALGHPIG
CHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCC
ASGARIIVTLLAALRHRQLKRGLAAICIGGGEATAIAIEFLN
CCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEECC
>Mature Secondary Structure 
SIERTPPHQDPVVIVSAVRTPMGGFQGDLQSLSATALGSIAIRAAVERAGIEPADVEHV
CCCCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LFGCVLPAGLGQAPARQAALGAGLSHATLCSTVNKMCGSGMQSAIMAHDLLLADSTAVAV
HHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEE
AGGMESMSNAPYLLDRARSGYRMGHGKVLDHMFLDGLEDAYEPGRLMGTFAEDCAGLNGF
ECCHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
TREAQDAFALASLARAQKAIAEGHFDAEIVPVQVTVGKESRQITHDEQPPKARPDKIPTL
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
KPAFREGGTVTAANSSSISDGAAALLLMRQSEAQRRGLRPLAVFHAHAAFADEPGLFPTA
CHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC
PIGAIQRLLGKTGWSLDDVDLFEINEAFAVVPLVAMRELGIPHDKVNVNGGACALGHPIG
CHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCC
ASGARIIVTLLAALRHRQLKRGLAAICIGGGEATAIAIEFLN
CCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]